##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552529_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	642838
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.597282674639644	34.0	31.0	34.0	31.0	34.0
2	32.74661578811458	34.0	31.0	34.0	31.0	34.0
3	32.78896082683351	34.0	31.0	34.0	31.0	34.0
4	36.17575034456581	37.0	37.0	37.0	35.0	37.0
5	36.212764335649105	37.0	37.0	37.0	35.0	37.0
6	36.24578665231365	37.0	37.0	37.0	35.0	37.0
7	36.2856567284448	37.0	37.0	37.0	35.0	37.0
8	36.28129948758474	37.0	37.0	37.0	35.0	37.0
9	37.78499248644293	39.0	38.0	39.0	35.0	39.0
10	37.85624216365554	39.0	38.0	39.0	35.0	39.0
11	37.811910621338505	39.0	38.0	39.0	35.0	39.0
12	37.65846760770209	39.0	37.0	39.0	35.0	39.0
13	37.61428384756346	39.0	37.0	39.0	35.0	39.0
14	38.92748250725688	40.0	38.0	41.0	35.0	41.0
15	39.00008711370516	40.0	38.0	41.0	35.0	41.0
16	39.050513504179904	40.0	38.0	41.0	35.0	41.0
17	39.04948680694047	40.0	38.0	41.0	35.0	41.0
18	39.04103833314148	40.0	38.0	41.0	35.0	41.0
19	39.01711939866654	40.0	38.0	41.0	35.0	41.0
20	38.96570208979556	40.0	38.0	41.0	35.0	41.0
21	38.894542637491874	40.0	38.0	41.0	35.0	41.0
22	38.90327578643453	40.0	38.0	41.0	35.0	41.0
23	38.86528332177003	40.0	38.0	41.0	35.0	41.0
24	38.850319987306285	40.0	38.0	41.0	35.0	41.0
25	38.77732025798102	40.0	38.0	41.0	35.0	41.0
26	38.73899645011652	40.0	38.0	41.0	35.0	41.0
27	38.7168089005317	40.0	38.0	41.0	35.0	41.0
28	38.66135947159315	40.0	38.0	41.0	35.0	41.0
29	38.61184777502263	40.0	38.0	41.0	35.0	41.0
30	38.538446700412855	40.0	37.0	41.0	35.0	41.0
31	38.47379744196827	40.0	37.0	41.0	35.0	41.0
32	38.3776861355427	40.0	37.0	41.0	35.0	41.0
33	38.29760997327476	40.0	37.0	41.0	35.0	41.0
34	38.1955298224436	40.0	37.0	41.0	34.0	41.0
35	38.123430786605645	40.0	37.0	41.0	34.0	41.0
36	38.09115360324063	40.0	37.0	41.0	34.0	41.0
37	38.040285421832564	40.0	36.0	41.0	34.0	41.0
38	37.964561833619044	40.0	36.0	41.0	34.0	41.0
39	37.91289718404948	40.0	36.0	41.0	34.0	41.0
40	37.829017575190015	40.0	36.0	41.0	34.0	41.0
41	37.7815561618946	40.0	36.0	41.0	34.0	41.0
42	37.73881133349304	40.0	35.0	41.0	34.0	41.0
43	37.686743783037095	40.0	35.0	41.0	34.0	41.0
44	37.60055099418516	40.0	35.0	41.0	34.0	41.0
45	37.54158746060438	40.0	35.0	41.0	34.0	41.0
46	37.45922767477965	39.0	35.0	41.0	33.0	41.0
47	37.39344904937169	39.0	35.0	41.0	33.0	41.0
48	37.33013449733836	39.0	35.0	41.0	33.0	41.0
49	37.29137045414241	39.0	35.0	41.0	33.0	41.0
50	37.20421630332992	39.0	35.0	41.0	33.0	41.0
51	36.59913072967062	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	8.0
13	7.0
14	7.0
15	19.0
16	16.0
17	44.0
18	70.0
19	110.0
20	209.0
21	344.0
22	553.0
23	1011.0
24	1634.0
25	2254.0
26	2639.0
27	3147.0
28	3503.0
29	4301.0
30	5179.0
31	6607.0
32	9009.0
33	13054.0
34	29624.0
35	52778.0
36	37010.0
37	57099.0
38	110418.0
39	301231.0
40	946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.55504497245029	20.913200526415675	25.672875592295412	10.85887890883862
2	31.35113356708844	25.06012401258171	26.170512633042854	17.418229787286997
3	31.807547158070932	24.681801635870933	26.1051773541701	17.405473851888033
4	29.59128116259462	26.12695578046102	25.38166692074831	18.90009613619606
5	25.852547609195476	29.94875847414123	23.89777828939795	20.300915627265343
6	27.650045579135025	33.56086603467748	23.128688720953026	15.660399665234475
7	79.38220827020183	7.261082885579259	8.124908608389672	5.231800235829245
8	80.6324454994882	5.9518883451196105	7.574381103792868	5.841285051599314
9	74.53230829540257	7.841633506419969	10.13365731335111	7.492400884826349
10	41.53628130259879	24.329302250333676	17.400962606442057	16.733453840625476
11	31.867593390558742	26.040308755860732	22.389777828939796	19.702320024640734
12	27.50615240542718	23.79728640808415	28.28986463152458	20.406696554964082
13	25.095125054834966	25.125459291454455	29.84126638437678	19.9381492693338
14	20.516988728108792	27.86689648091743	28.606429613681826	23.009685177291946
15	19.749143641166203	25.970462231542008	32.58285913402755	21.697534993264245
16	21.891829667816776	25.15034892150122	30.448573357517756	22.50924805316425
17	22.119258662369056	25.019056123004553	27.7911386694626	25.07054654516379
18	22.755188710063813	24.642600468547286	29.561258046350712	23.04095277503819
19	23.511055662546397	26.69055034083237	27.29925735566348	22.499136640957754
20	25.134637342534198	26.960291706464147	27.136385839044983	20.768685111956668
21	23.922667919444713	27.32959159228297	27.12565218608732	21.622088302185
22	22.01690005880175	25.98881833370149	26.09895494665841	25.895326660838347
23	21.52999667101198	27.65782358852464	26.636259835292876	24.17591990517051
24	22.77230033072096	25.595251058587078	27.56853204073188	24.063916569960085
25	21.261499786882542	27.60213304129501	26.16973483210389	24.96663233971856
26	20.384762568485375	29.49607832766576	26.748418730691093	23.370740373157776
27	21.94798689560978	27.97781089481331	26.282204847877694	23.791997361699217
28	19.846368758536364	28.33388816466979	27.72471446927531	24.095028607518536
29	21.84547273185468	27.550331498760183	26.41303096581098	24.19116480357415
30	22.917437985931137	27.228633030405796	25.81692432619105	24.03700465747202
31	22.668697245651316	29.21249210532047	23.939935100289652	24.178875548738564
32	23.374784938040378	28.76478988485435	23.88735575681587	23.973069420289402
33	23.46314312470638	26.356562617642393	24.514263313618674	25.666030944032553
34	20.91848957280061	27.822872947772222	26.86384439003295	24.394793089394216
35	21.930408594389256	26.53841247717154	25.975595717739154	25.55558321070005
36	22.766544603772644	28.636141609550155	25.209461792862275	23.387851993814927
37	22.59325055457207	28.252063505891062	26.292938500835355	22.861747438701506
38	23.089643113817168	27.770604724674026	25.52307113145147	23.61668103005734
39	24.62362212563663	24.40739346460539	28.21332901913079	22.75565539062719
40	23.340717256913873	23.7440848238592	29.738441100246092	23.17675681898083
41	20.738506435524968	24.879051953991517	27.232366474912805	27.150075135570706
42	23.014818663489088	25.181927639623048	26.233047828535337	25.570205868352524
43	23.979758508364473	24.264900332587683	26.785753175761233	24.969587983286615
44	22.43971264922111	25.490092371639513	26.820443097638908	25.249751881500472
45	23.14968934630498	23.564257246771348	27.40254932035754	25.883504086566134
46	22.30826429053665	25.155482407698365	27.601199680168254	24.93505362159673
47	20.92128965618087	25.59151761408006	29.457343840905487	24.02984888883358
48	21.9616761921355	23.990336601134345	28.401401286171634	25.646585920558522
49	23.23866977372215	23.097110002831194	29.394030844474035	24.270189378972617
50	21.16551915101472	23.132577725647828	29.130667446541743	26.571235676795705
51	21.13316263195393	22.995373640015057	26.61074796449494	29.260715763536073
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	228.0
1	450.5
2	673.0
3	1109.5
4	1546.0
5	1167.5
6	789.0
7	822.5
8	856.0
9	938.5
10	1021.0
11	1100.5
12	1180.0
13	1234.5
14	1289.0
15	1279.0
16	1269.0
17	1420.5
18	1572.0
19	1486.0
20	1400.0
21	1595.0
22	1790.0
23	1994.0
24	2198.0
25	3486.0
26	5363.5
27	5953.0
28	6855.5
29	7758.0
30	8940.5
31	10123.0
32	10970.5
33	11818.0
34	13286.0
35	14754.0
36	15808.5
37	16863.0
38	18212.0
39	19561.0
40	22200.5
41	24840.0
42	28392.0
43	31944.0
44	35700.5
45	39457.0
46	46123.5
47	52790.0
48	59903.5
49	67017.0
50	67907.0
51	68797.0
52	60561.5
53	52326.0
54	46180.0
55	40034.0
56	35767.0
57	31500.0
58	29452.5
59	27405.0
60	26518.5
61	25632.0
62	23237.5
63	20843.0
64	18573.5
65	16304.0
66	13725.0
67	11146.0
68	9487.5
69	7829.0
70	6647.0
71	5465.0
72	5111.0
73	4757.0
74	3861.0
75	2630.0
76	2295.0
77	1695.5
78	1096.0
79	844.0
80	592.0
81	407.0
82	222.0
83	167.0
84	112.0
85	75.5
86	39.0
87	22.5
88	6.0
89	3.5
90	1.0
91	3.5
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	642838.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.288972271789206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.3767652835228	20.710619477072605
2	12.111426362562597	7.3368531453495045
3	4.511434583449521	4.099401510122802
4	2.2030549001731647	2.6691307513829727
5	1.3675890705365377	2.071143371834158
6	1.0287559837449847	1.869597687965344
7	0.8019125934804383	1.7002375815829296
8	0.6922630419312626	1.6774348865472368
9	0.6260593394596731	1.7066424576049832
>10	8.110481842267484	48.82958410456818
>50	0.10956115033135276	2.1891158802419963
>100	0.054523389366576566	2.928376776379573
>500	0.0030862295867873524	0.6488981311779594
>1k	0.0030862295867873524	1.5629642381698168
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	2941	0.4575025122970328	No Hit
CTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCT	1988	0.3092536533310103	No Hit
CCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	1646	0.2560520691060578	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1208	0.1879167068530485	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1175	0.18278322065590397	No Hit
TCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	1074	0.16707164168888586	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCC	984	0.15307122478758256	No Hit
TGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCTT	802	0.12475927060939147	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	681	0.10593648788652817	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3159427414060774	0.0
2	0.0	0.0	0.0	1.304216614450297	0.0
3	0.0	0.0	0.0	1.806987141394877	0.0
4	0.0	0.0	0.0	2.376804109277921	0.0
5	0.0	0.0	0.0	3.9070496765903697	0.0
6	0.0	0.0	0.0	4.799809594330142	0.0
7	0.0	0.0	0.0	5.75214906399435	0.0
8	0.0	0.0	0.0	7.276483344170693	0.0
9	0.0	0.0	0.0	7.975104147545727	0.0
10	0.0	0.0	0.0	9.606463836923144	0.0
11	1.555601877922587E-4	0.0	0.0	11.928044079534812	0.0
12	1.555601877922587E-4	0.0	0.0	13.657095566845769	0.0
13	1.555601877922587E-4	0.0	0.0	14.361938777732492	0.0
14	1.555601877922587E-4	0.0	0.0	14.661858819795967	0.0
15	1.555601877922587E-4	0.0	0.0	15.210364041951472	0.0
16	1.555601877922587E-4	0.0	0.0	16.28590718034715	0.0
17	1.555601877922587E-4	0.0	0.0	17.50907693695768	0.0
18	1.555601877922587E-4	0.0	0.0	18.897451613003586	0.0
19	1.555601877922587E-4	0.0	0.0	19.6808527187254	0.0
20	1.555601877922587E-4	0.0	0.0	20.45352017148955	0.0
21	1.555601877922587E-4	0.0	0.0	21.36930299702258	0.0
22	1.555601877922587E-4	0.0	0.0	22.25941839156988	0.0
23	1.555601877922587E-4	0.0	0.0	23.07953170161067	0.0
24	1.555601877922587E-4	0.0	0.0	23.75092947212206	0.0
25	3.111203755845174E-4	0.0	0.0	24.3750369455446	0.0
26	3.111203755845174E-4	0.0	0.0	24.91483079718374	0.0
27	4.666805633767761E-4	0.0	0.0	25.480136519620807	0.0
28	4.666805633767761E-4	0.0	0.0	26.028330621400727	0.0
29	4.666805633767761E-4	0.0	0.0	26.58508053350922	0.0
30	4.666805633767761E-4	0.0	0.0	27.203743400359034	0.0
31	4.666805633767761E-4	0.0	0.0	27.79020530833585	0.0
32	4.666805633767761E-4	0.0	0.0	28.348355262134472	0.0
33	6.222407511690348E-4	0.0	0.0	28.890326956402703	0.0
34	7.778009389612935E-4	0.0	0.0	29.408342381750924	0.0
35	7.778009389612935E-4	0.0	0.0	29.960269928037857	0.0
36	7.778009389612935E-4	0.0	0.0	30.46739614024062	0.0
37	9.333611267535522E-4	0.0	0.0	30.980122519203906	0.0
38	9.333611267535522E-4	0.0	0.0	31.496115662110828	0.0
39	9.333611267535522E-4	0.0	0.0	32.037931796191266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCAT	30	2.1640026E-6	45.000004	16
TTCGTAA	25	3.8888804E-5	45.0	42
TGCGACA	20	7.0309633E-4	45.0	27
ACATGCG	20	7.0309633E-4	45.0	1
GTAAGCG	20	7.0309633E-4	45.0	1
TATCGCG	20	7.0309633E-4	45.0	1
TAAGACG	25	3.8888804E-5	45.0	1
GGCACGA	55	6.002665E-11	40.909092	8
CACGAGG	50	1.0804797E-9	40.5	2
TCGGCGT	190	0.0	40.263157	4
ATTAGTC	40	3.455043E-7	39.375	31
CGCAATC	40	3.455043E-7	39.375	20
TAGGACG	40	3.455043E-7	39.375	1
CGCGAGG	75	0.0	39.0	2
ACGTTAG	35	6.243994E-6	38.571426	1
GCAACGA	35	6.243994E-6	38.571426	11
TTATGCG	35	6.243994E-6	38.571426	1
CGTTGAT	35	6.243994E-6	38.571426	25
TAAACGG	35	6.243994E-6	38.571426	2
CGCTACA	35	6.243994E-6	38.571426	28
>>END_MODULE