FastQCFastQC Report
Sat 18 Jun 2016
SRR3552527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594123
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGC56650.953506260488148No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCG49760.837537008329925No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC46020.7745870804530375No Hit
GCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC35110.5909550715929193No Hit
CCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC22810.38392723392294187No Hit
CTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGCT22720.3824123960863323No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCATCAAT13520.22756230612179634No Hit
TGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGCTT12570.21157235117980622Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCT11740.1976021800199622No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCC11720.1972655493896045No Hit
TCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC11380.19154282867352382No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCATCAATCGTA11300.1901963061520931No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA10520.1770677115681433No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG9540.16057281068061663No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTC8920.15013726113952836No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8290.13953339628326122No Hit
ACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC7540.12690974764484794No Hit
GAACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCT7320.12320681071091341No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7140.12017713503769423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG351.2106102E-745.0000041
ACGCATT351.2106102E-745.00000417
CCGATCG351.2106102E-745.00000440
GGACGTA351.2106102E-745.0000048
CTCTACG351.2106102E-745.00000413
ATTGTCG207.0305326E-445.01
CGATGTC253.888522E-545.010
TGTAACG207.0305326E-445.01
TCAACGG207.0305326E-445.02
GCATACT207.0305326E-445.045
TATAGCG302.1637243E-644.9999961
CGATCGA302.1637243E-644.99999641
CGAATAT800.042.187514
AGGGCGC501.0804797E-940.56
TATTGCG403.454461E-739.3751
ATGACGG403.454461E-739.3752
ATTGACG403.454461E-739.3751
GATGAAT5150.039.3203920
TGATCCG356.2431936E-638.571434
TCGTTAG356.2431936E-638.571431
Produced by FastQC (version 0.11.5)