FastQCFastQC Report
Sat 18 Jun 2016
SRR3552524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421896
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC23850.5653051936970249TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT21480.5091302121849934TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC14930.3538786810019531TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC12700.30102205282818517TruSeq Adapter, Index 20 (95% over 22bp)
TGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCTT9970.23631416273204772TruSeq Adapter, Index 20 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC7450.17658380264330545TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCC5340.1265714773309062No Hit
GCCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG4690.11116483683182585TruSeq Adapter, Index 20 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA4640.1099797106395889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG302.1622218E-645.0000041
CGGGTAT253.886594E-545.06
GTCGATG253.886594E-545.01
GATAACG207.028205E-445.01
GCGAATG207.028205E-445.01
AATTGCG207.028205E-445.01
GGTTGCG207.028205E-445.013
TAGGACG207.028205E-445.02
TATCGAG406.7975634E-945.01
TCGCGCG207.028205E-445.01
CTAGCGG453.8380676E-1045.02
CGTAAGG850.042.352942
GCGTTCA603.6379788E-1241.25000437
TTACACG2100.040.71428734
TATTAGG850.039.7058832
GGGACCG1250.039.67
ACAACGA1200.039.37500413
CACAACG1200.039.37500412
CGTGCGG403.4512777E-739.3752
TCGGCGT800.039.3754
Produced by FastQC (version 0.11.5)