##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552524_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421896
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54685514913628	34.0	31.0	34.0	31.0	34.0
2	32.69366384132582	34.0	31.0	34.0	31.0	34.0
3	32.73003062366081	34.0	31.0	34.0	31.0	34.0
4	36.13809564442422	37.0	37.0	37.0	35.0	37.0
5	36.18145230104101	37.0	35.0	37.0	35.0	37.0
6	36.227767032633636	37.0	37.0	37.0	35.0	37.0
7	36.3049732635531	37.0	37.0	37.0	35.0	37.0
8	36.30958340444091	37.0	37.0	37.0	35.0	37.0
9	37.81374319737566	39.0	39.0	39.0	35.0	39.0
10	37.84675133208184	39.0	38.0	39.0	35.0	39.0
11	37.795131975652765	39.0	37.0	39.0	35.0	39.0
12	37.69758423876974	39.0	37.0	39.0	35.0	39.0
13	37.67499573354571	39.0	37.0	39.0	35.0	39.0
14	38.998347934088024	40.0	38.0	41.0	35.0	41.0
15	39.042439368944	40.0	38.0	41.0	35.0	41.0
16	39.091505963555	40.0	38.0	41.0	35.0	41.0
17	39.081266473254075	40.0	38.0	41.0	35.0	41.0
18	39.080887232872556	40.0	38.0	41.0	35.0	41.0
19	39.06055283766616	40.0	38.0	41.0	35.0	41.0
20	39.02651127292034	40.0	38.0	41.0	35.0	41.0
21	38.95147145268028	40.0	38.0	41.0	35.0	41.0
22	38.95670971044997	40.0	38.0	41.0	35.0	41.0
23	38.91464010087794	40.0	38.0	41.0	35.0	41.0
24	38.88863369171549	40.0	38.0	41.0	35.0	41.0
25	38.841010580806646	40.0	38.0	41.0	35.0	41.0
26	38.80902402487817	40.0	38.0	41.0	35.0	41.0
27	38.786440260158905	40.0	38.0	41.0	35.0	41.0
28	38.74179181599256	40.0	38.0	41.0	35.0	41.0
29	38.72240315148757	40.0	38.0	41.0	35.0	41.0
30	38.6592264444318	40.0	38.0	41.0	35.0	41.0
31	38.592828564385535	40.0	38.0	41.0	35.0	41.0
32	38.491929290630864	40.0	38.0	41.0	35.0	41.0
33	38.411056279272614	40.0	38.0	41.0	35.0	41.0
34	38.32397083641466	40.0	38.0	41.0	35.0	41.0
35	38.282891992339344	40.0	38.0	41.0	35.0	41.0
36	38.27445863435538	40.0	37.0	41.0	35.0	41.0
37	38.245641105864955	40.0	37.0	41.0	35.0	41.0
38	38.188489580370515	40.0	37.0	41.0	34.0	41.0
39	38.14136422246241	40.0	37.0	41.0	34.0	41.0
40	38.073904469347895	40.0	37.0	41.0	34.0	41.0
41	38.01533790317993	40.0	37.0	41.0	34.0	41.0
42	37.97463355898136	40.0	37.0	41.0	34.0	41.0
43	37.91936875438497	40.0	36.0	41.0	34.0	41.0
44	37.84641712649563	40.0	36.0	41.0	34.0	41.0
45	37.79555862108197	40.0	36.0	41.0	34.0	41.0
46	37.70698228947418	40.0	36.0	41.0	34.0	41.0
47	37.64026916586078	40.0	35.0	41.0	34.0	41.0
48	37.59168136223145	40.0	35.0	41.0	34.0	41.0
49	37.55333541915543	40.0	35.0	41.0	34.0	41.0
50	37.47807042493885	40.0	35.0	41.0	34.0	41.0
51	36.840387204429526	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	4.0
14	1.0
15	9.0
16	7.0
17	20.0
18	55.0
19	84.0
20	144.0
21	267.0
22	418.0
23	691.0
24	1106.0
25	1491.0
26	1818.0
27	2000.0
28	2225.0
29	2619.0
30	3309.0
31	4229.0
32	5719.0
33	8271.0
34	17485.0
35	31352.0
36	22366.0
37	34656.0
38	69721.0
39	211201.0
40	624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.78494226065192	20.64584636972145	26.865625651819407	10.703585717807233
2	30.898373058763294	24.963972163755997	26.78717029789332	17.350484479587386
3	31.95716479890779	24.306701177541385	26.563181447560535	17.17295257599029
4	29.66133833930637	25.68215863625159	26.080835087320096	18.575667937121946
5	25.269023645637787	29.478354855224985	24.997629747615527	20.254991751521704
6	27.521000436126435	33.059569182926595	23.34627491135256	16.073155469594404
7	79.49968712668525	5.987731573657963	9.055312209644082	5.457269090012704
8	79.82583385478885	5.130885336670649	8.62060792233157	6.422672886208923
9	73.58448527599218	7.635056980867323	11.22764852001441	7.5528092231260775
10	38.31655194645126	29.44777859946527	17.91531562280752	14.320353831275954
11	27.119479682196562	26.395604603978228	27.288004626732654	19.196911087092552
12	24.532586229781746	23.133426247226804	32.26671976032008	20.06726776267137
13	23.17822401729336	25.000474050476896	32.75262149913723	19.068680433092517
14	19.86650738570643	28.434258679864232	30.065466370859166	21.63376756357017
15	18.359500919657926	26.206221438458766	34.706420539659064	20.727857102224245
16	20.709132108386903	26.836945597967272	31.17427043631606	21.279651857329767
17	20.78118778087491	25.748762728255304	28.63620418302141	24.833845307848378
18	21.218736371048788	25.28182300851395	32.123082465820964	21.376358154616305
19	22.1530898610084	27.15124106414851	29.37998938126932	21.31567969357377
20	23.664599806587404	27.600166865767868	29.12660940136906	19.60862392627567
21	21.950907332612775	28.0694768378937	29.693810796973665	20.285805032519864
22	20.65034984925195	26.350332783434784	28.053596146917727	24.945721220395548
23	20.134819955628874	27.65705292299524	28.803544001365267	23.40458312001062
24	20.90278172819842	26.110937292602916	28.933196806795987	24.053084172402677
25	19.083375997876253	28.32096061588638	28.139873333712572	24.455790052524794
26	18.893755807118342	29.59876367635626	28.540683011927108	22.96679750459829
27	20.83641466143315	27.686444052562713	28.363388133568463	23.11375315243567
28	18.832603295598915	27.520763410887994	29.09745529704003	24.549177996473066
29	21.813669719551736	26.43021028879155	27.467669757475775	24.288450234180935
30	21.821965602897397	26.572425431859987	26.422151430684337	25.18345753455828
31	22.668382729393024	28.63691525873675	25.040057265297612	23.654644746572615
32	22.55698086732275	28.829142727117585	25.56459411798168	23.04928228757798
33	21.79968523048334	26.448461232152	25.553453931774655	26.198399605590005
34	21.391764795115385	25.433756186358725	29.47077004759467	23.703708970931224
35	20.29884142063447	26.194133151297947	28.04055975880312	25.466465669264466
36	21.210203462464683	28.5871399586628	26.469556478373818	23.733100100498703
37	23.05497089330072	26.25054515804843	28.933196806795987	21.761287141854865
38	22.808464645315432	26.98958985152739	26.491125775072526	23.710819728084648
39	23.260708800273054	25.003081328099817	27.714413030699507	24.02179684092762
40	21.709852665111782	23.75680262434344	31.60565637029031	22.92768834025447
41	19.345999962075965	26.334689117697252	28.854741452869902	25.464569467356885
42	21.51312171720045	26.070405976828415	28.94647022014904	23.4700020858221
43	23.247672412158447	24.53424540645088	27.672222538255873	24.5458596431348
44	22.832167169160172	24.036255380472912	26.795940231715875	26.335637218651044
45	23.099294612890382	21.924360505906666	27.799505091302123	27.176839789900832
46	21.757968788516603	24.232275252668902	29.179703054781275	24.83005290403322
47	18.836158674175625	24.93742533704989	32.69194303809469	23.53447295067979
48	20.107562053207424	23.818192161101315	31.18161821870793	24.892627566983332
49	22.012770919847547	22.298149306938203	31.287331475055463	24.401748298158786
50	20.803942203765857	21.612435292109904	30.592610501166163	26.991012002958076
51	19.81578398467869	21.38489106320041	28.882236380529797	29.917088571591105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	140.0
1	260.5
2	381.0
3	635.0
4	889.0
5	680.5
6	472.0
7	500.5
8	529.0
9	619.0
10	709.0
11	714.0
12	719.0
13	768.0
14	817.0
15	832.0
16	847.0
17	917.5
18	988.0
19	1138.0
20	1288.0
21	1469.5
22	1651.0
23	1797.0
24	1943.0
25	2398.5
26	3434.5
27	4015.0
28	4869.5
29	5724.0
30	6014.0
31	6304.0
32	7044.0
33	7784.0
34	9092.0
35	10400.0
36	11227.5
37	12055.0
38	13427.5
39	14800.0
40	16177.0
41	17554.0
42	20062.5
43	22571.0
44	26154.5
45	29738.0
46	35457.0
47	41176.0
48	47002.5
49	52829.0
50	51517.5
51	50206.0
52	42813.0
53	35420.0
54	30508.0
55	25596.0
56	22099.5
57	18603.0
58	16425.5
59	14248.0
60	12961.0
61	11674.0
62	10514.0
63	9354.0
64	7963.0
65	6572.0
66	5173.0
67	3774.0
68	3167.5
69	2561.0
70	2121.0
71	1681.0
72	1370.5
73	1060.0
74	1000.5
75	731.0
76	521.0
77	384.0
78	247.0
79	193.0
80	139.0
81	103.0
82	67.0
83	57.5
84	48.0
85	26.5
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	421896.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.820096098047074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.98097080673547	23.671039363290618
2	13.025058623889475	9.070675859330564
3	4.866475003927035	5.083533818864501
4	2.517206530067794	3.5059749310236885
5	1.451460857574633	2.5270003271651267
6	1.092941061854671	2.283378768196875
7	0.8879607097073531	2.164321407031008
8	0.7697826959898595	2.144312595918453
9	0.6948418101841887	2.1775012743198987
>10	6.580815483616721	40.17758798713466
>50	0.07580408364053456	1.7829740600409558
>100	0.051901895105230876	3.135542272032548
>500	0.002048759017311745	0.5412175194256088
>1k	0.002731678689748993	1.734939816225502
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	2385	0.5653051936970249	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	2148	0.5091302121849934	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	1493	0.3538786810019531	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	1270	0.30102205282818517	TruSeq Adapter, Index 20 (95% over 22bp)
TGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCTT	997	0.23631416273204772	TruSeq Adapter, Index 20 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	745	0.17658380264330545	TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCC	534	0.1265714773309062	No Hit
GCCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG	469	0.11116483683182585	TruSeq Adapter, Index 20 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	464	0.1099797106395889	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5980146766027646	0.0
2	0.0	0.0	0.0	2.235385023797334	0.0
3	0.0	0.0	0.0	3.0201755882966417	0.0
4	0.0	0.0	0.0	3.9324857310806456	0.0
5	0.0	0.0	0.0	6.061683448053548	0.0
6	0.0	0.0	0.0	7.117156834859776	0.0
7	0.0	0.0	0.0	8.325037449987674	0.0
8	0.0	0.0	0.0	10.20962512088287	0.0
9	0.0	0.0	0.0	10.980431196313784	0.0
10	0.0	0.0	0.0	12.865255892447427	0.0
11	0.0	0.0	0.0	15.61214138081423	0.0
12	0.0	0.0	0.0	17.726643533003394	0.0
13	0.0	0.0	0.0	18.537269848493466	0.0
14	0.0	0.0	0.0	18.87029030851205	0.0
15	0.0	0.0	0.0	19.48679295371371	0.0
16	0.0	0.0	0.0	20.722642546978403	0.0
17	0.0	0.0	0.0	22.285823994538937	0.0
18	0.0	0.0	0.0	24.080342074824127	0.0
19	0.0	0.0	0.0	25.093861994425165	0.0
20	0.0	0.0	0.0	26.055473386806227	0.0
21	2.3702523844738987E-4	0.0	0.0	27.155270493202117	0.0
22	2.3702523844738987E-4	0.0	0.0	28.263363482943664	0.0
23	0.0011851261922369493	0.0	0.0	29.305326431158388	0.0
24	0.0011851261922369493	0.0	0.0	30.123300529040332	0.0
25	0.0033183533382634585	0.0	0.0	30.847412632497107	0.0
26	0.0033183533382634585	0.0	0.0	31.506105770142405	0.0
27	0.0033183533382634585	0.0	0.0	32.15034036824241	0.0
28	0.0033183533382634585	0.0	0.0	32.81756641447181	0.0
29	0.0033183533382634585	0.0	0.0	33.49640669738514	0.0
30	0.0033183533382634585	0.0	0.0	34.25630961184747	0.0
31	0.0033183533382634585	0.0	0.0	34.972125831958586	0.0
32	0.0033183533382634585	0.0	0.0	35.65404744297173	0.0
33	0.0033183533382634585	0.0	0.0	36.33122854921592	0.0
34	0.0033183533382634585	0.0	0.0	36.93516885677987	0.0
35	0.0042664542920530174	0.0	0.0	37.607372433016664	0.0
36	0.004503479530500408	0.0	0.0	38.23738551680983	0.0
37	0.004503479530500408	0.0	0.0	38.855310313442175	0.0
38	0.004503479530500408	0.0	0.0	39.480819917704835	0.0
39	0.004503479530500408	0.0	0.0	40.106092496729055	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	30	2.1622218E-6	45.000004	1
CGGGTAT	25	3.886594E-5	45.0	6
GTCGATG	25	3.886594E-5	45.0	1
GATAACG	20	7.028205E-4	45.0	1
GCGAATG	20	7.028205E-4	45.0	1
AATTGCG	20	7.028205E-4	45.0	1
GGTTGCG	20	7.028205E-4	45.0	13
TAGGACG	20	7.028205E-4	45.0	2
TATCGAG	40	6.7975634E-9	45.0	1
TCGCGCG	20	7.028205E-4	45.0	1
CTAGCGG	45	3.8380676E-10	45.0	2
CGTAAGG	85	0.0	42.35294	2
GCGTTCA	60	3.6379788E-12	41.250004	37
TTACACG	210	0.0	40.714287	34
TATTAGG	85	0.0	39.705883	2
GGGACCG	125	0.0	39.6	7
ACAACGA	120	0.0	39.375004	13
CACAACG	120	0.0	39.375004	12
CGTGCGG	40	3.4512777E-7	39.375	2
TCGGCGT	80	0.0	39.375	4
>>END_MODULE