FastQCFastQC Report
Sat 18 Jun 2016
SRR3552520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412386
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC26740.6484216244004404RNA PCR Primer, Index 3 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT24450.5928911262748978TruSeq Adapter, Index 3 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC23650.5734918256196865RNA PCR Primer, Index 24 (95% over 23bp)
TGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCTT14420.3496723943101851RNA PCR Primer, Index 24 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC13240.32105842584374833RNA PCR Primer, Index 24 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG10930.2650429452018255No Hit
ACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC8620.20902746455990262TruSeq Adapter, Index 3 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA8400.2036926568797195No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG7360.1784735660279447No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC7210.17483619715509255No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC7000.16974388073309957No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6840.1658640206020573No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC6470.15689184404902204No Hit
GCCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG5950.14428229862313463RNA PCR Primer, Index 24 (95% over 22bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC5600.13579510458647967No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG4300.10427124102176118TruSeq Adapter, Index 3 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCA207.028018E-445.00000439
CACGTAG207.028018E-445.0000041
ACGACCG207.028018E-445.0000041
CTGGACG207.028018E-445.0000041
ATACACG207.028018E-445.00000427
GCGAGTT207.028018E-445.0000041
CATGCGG253.8864397E-545.02
GCGAAAG502.1827873E-1145.01
ATCAATT351.2093915E-745.017
TACGCGG351.2093915E-745.02
CATACGG253.8864397E-545.02
GCGATAC253.8864397E-545.09
GTATCAA253.8864397E-545.015
TACAGCG253.8864397E-545.01
GTTACGG302.1621036E-644.9999962
TAATCGT302.1621036E-644.99999621
TCGGCGT1650.042.2727284
CTTGCGG603.6379788E-1241.2499962
TCTTAGA1650.040.9090920
AGTGGCG1650.040.9090933
Produced by FastQC (version 0.11.5)