##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552520_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412386
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.566340758415656	34.0	31.0	34.0	31.0	34.0
2	32.71067883002818	34.0	31.0	34.0	31.0	34.0
3	32.75415266279651	34.0	31.0	34.0	31.0	34.0
4	36.1418476863909	37.0	37.0	37.0	35.0	37.0
5	36.194373717827474	37.0	37.0	37.0	35.0	37.0
6	36.23090017604866	37.0	37.0	37.0	35.0	37.0
7	36.2797839887872	37.0	37.0	37.0	35.0	37.0
8	36.280487213435954	37.0	37.0	37.0	35.0	37.0
9	37.805485637242775	39.0	39.0	39.0	35.0	39.0
10	37.87686051417846	39.0	38.0	39.0	35.0	39.0
11	37.8132138336413	39.0	37.0	39.0	35.0	39.0
12	37.64295829635341	39.0	37.0	39.0	35.0	39.0
13	37.596482421808695	39.0	37.0	39.0	35.0	39.0
14	38.91196112380149	40.0	38.0	41.0	35.0	41.0
15	38.96523887813844	40.0	38.0	41.0	35.0	41.0
16	38.99828316189201	40.0	38.0	41.0	35.0	41.0
17	38.97210865548297	40.0	38.0	41.0	35.0	41.0
18	38.96320922630739	40.0	38.0	41.0	35.0	41.0
19	38.95524338847584	40.0	38.0	41.0	35.0	41.0
20	38.911330646530196	40.0	38.0	41.0	35.0	41.0
21	38.84659275533117	40.0	38.0	41.0	35.0	41.0
22	38.83845717361889	40.0	38.0	41.0	35.0	41.0
23	38.810124495011955	40.0	38.0	41.0	35.0	41.0
24	38.79192552608478	40.0	38.0	41.0	35.0	41.0
25	38.751368863152486	40.0	38.0	41.0	35.0	41.0
26	38.706949314477214	40.0	38.0	41.0	35.0	41.0
27	38.68100274985087	40.0	38.0	41.0	35.0	41.0
28	38.62389848345967	40.0	38.0	41.0	35.0	41.0
29	38.58977268869457	40.0	37.0	41.0	35.0	41.0
30	38.50314996144389	40.0	37.0	41.0	35.0	41.0
31	38.4358222636074	40.0	37.0	41.0	35.0	41.0
32	38.34643028618818	40.0	37.0	41.0	35.0	41.0
33	38.27726207970203	40.0	37.0	41.0	35.0	41.0
34	38.160953572623704	40.0	37.0	41.0	34.0	41.0
35	38.113270091613195	40.0	37.0	41.0	34.0	41.0
36	38.0857764327596	40.0	37.0	41.0	34.0	41.0
37	38.02091972084406	40.0	36.0	41.0	34.0	41.0
38	37.95734821259693	40.0	36.0	41.0	34.0	41.0
39	37.95268268078936	40.0	36.0	41.0	34.0	41.0
40	37.88446504003531	40.0	36.0	41.0	34.0	41.0
41	37.83698767659426	40.0	36.0	41.0	34.0	41.0
42	37.79220681594428	40.0	35.0	41.0	34.0	41.0
43	37.732520017653364	40.0	35.0	41.0	34.0	41.0
44	37.6602309486743	40.0	35.0	41.0	34.0	41.0
45	37.62499211903411	40.0	35.0	41.0	34.0	41.0
46	37.551262651981396	40.0	35.0	41.0	34.0	41.0
47	37.48842589224658	39.0	35.0	41.0	34.0	41.0
48	37.43674373038852	39.0	35.0	41.0	34.0	41.0
49	37.40446571901083	39.0	35.0	41.0	34.0	41.0
50	37.33489497703608	39.0	35.0	41.0	34.0	41.0
51	36.75681521681143	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	2.0
14	3.0
15	7.0
16	13.0
17	28.0
18	43.0
19	74.0
20	133.0
21	247.0
22	424.0
23	655.0
24	931.0
25	1304.0
26	1706.0
27	1861.0
28	2246.0
29	2546.0
30	3176.0
31	4149.0
32	5911.0
33	8320.0
34	19394.0
35	35663.0
36	23843.0
37	37128.0
38	68520.0
39	193399.0
40	657.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.50047285795347	19.797956283675973	26.244343891402718	11.457226966967841
2	31.15018453584748	25.02825023157915	26.04477358591223	17.776791646661138
3	32.40168191936681	23.721222349934283	27.00576644211976	16.871329288579144
4	29.64989112142507	25.19362926966483	26.244343891402718	18.912135717507383
5	26.62990499192504	28.354260328915142	25.070928693020615	19.9449059861392
6	26.674038400915645	33.48707279102589	22.89044729937486	16.94844150868361
7	76.89543291964324	7.309171504367268	9.90552540580912	5.889870170180365
8	77.24074047130601	6.626316121303827	9.026009612353475	7.1069337950366895
9	71.89623314079527	7.6964300436969255	11.721784929653287	8.685551885854515
10	41.59185811351501	23.301227490748957	18.98924793761185	16.117666458124187
11	31.99308414931642	25.48995358717318	22.341932073348755	20.175030190161642
12	27.281236511423764	22.97822913483969	29.795385876339157	19.94514847739739
13	24.918159200360826	25.23242787097525	30.1215366186049	19.727876310059024
14	20.08458095085672	28.437434830474363	28.158327392297505	23.31965682637141
15	20.360778493935292	25.417933683490713	32.51613779323255	21.705150029341443
16	22.124174923494007	26.079207344575227	29.363023962986134	22.43359376894463
17	22.052882493586107	26.025131793998824	26.705319773222175	25.21666593919289
18	22.442808436755854	25.130096560019012	30.295160359469044	22.131934643756093
19	23.702308031795454	26.398325840353454	27.392055016416656	22.507311111434433
20	24.492102059720747	26.964057945711055	28.203915748837254	20.33992424573094
21	23.56918033104907	28.238349507500253	27.73809004185399	20.45438011959669
22	21.954188551502718	26.110488716881758	26.90537506122904	25.02994767038648
23	21.814513586785196	26.555945158177046	27.489294010950903	24.14024724408685
24	22.867168138588603	24.99187654285063	28.332921098194408	23.808034220366356
25	21.07006542414146	26.52175389077224	27.00382651205424	25.40435417303206
26	19.73903090793577	26.889128146930304	29.669290422080287	23.702550523053645
27	23.95571139660415	25.544271629007774	27.940570242442757	22.55944673194531
28	19.358804615093625	26.37237927572711	29.753192397414075	24.51562371176519
29	23.935827113432563	24.582793790283862	28.287575232912854	23.193803863370725
30	23.98553782136154	25.949959503959878	26.635239799605227	23.429262875073352
31	24.212267147769325	26.870698811307854	25.566095842244884	23.350938198677937
32	24.778241744385117	26.497504764953224	26.574132002541305	22.15012148812035
33	25.240187591237333	25.254737066728744	24.917674217844446	24.587401124189473
34	21.406400799251188	25.662122380488185	29.723850955173063	23.207625865087564
35	23.094867430029147	23.38585693985732	29.66298564936734	23.856289980746194
36	25.138098771539287	26.107093839267094	27.731785269141046	21.023022120052573
37	24.352427095003225	26.40026577041898	29.46753769526609	19.77976943931171
38	23.273098504798902	26.410692894521155	26.61414306014268	23.702065540537266
39	25.284078508969753	24.34151498838467	26.541153191427448	23.83325331121813
40	23.324991634051592	24.12642524237001	29.150359129553376	23.398223994025017
41	21.63676749453182	24.749870267176867	28.224527505783414	25.388834732507892
42	23.806094290300837	22.494701566008544	30.18385687195976	23.515347271730853
43	25.670367083266648	21.63434258194992	28.996134689344448	23.69915564543898
44	22.938218077238314	22.55580936307246	28.726726901495205	25.77924565819402
45	23.50952748153429	22.272094590989994	27.454132778513333	26.764245148962377
46	23.378097219595233	24.227544097035302	28.38917907009452	24.00517961327494
47	20.92069080909633	24.05198042610564	31.83134248010359	23.195986284694435
48	22.11180786932631	22.783023671996624	29.923906243179932	25.18126221549713
49	23.239877202426854	21.029326892765514	31.534048197562477	24.19674770724515
50	22.052640002327916	21.65422686512151	30.12105163608852	26.172081496462052
51	21.509459583981997	21.177489051519693	28.137230652834965	29.17582071166335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	184.0
1	339.0
2	494.0
3	710.0
4	926.0
5	703.5
6	481.0
7	484.0
8	487.0
9	530.0
10	573.0
11	646.5
12	720.0
13	715.0
14	710.0
15	728.0
16	746.0
17	786.5
18	827.0
19	782.5
20	738.0
21	881.0
22	1024.0
23	1179.5
24	1335.0
25	2190.0
26	3567.0
27	4089.0
28	4935.5
29	5782.0
30	6120.0
31	6458.0
32	7556.5
33	8655.0
34	8119.5
35	7584.0
36	8658.5
37	9733.0
38	10469.5
39	11206.0
40	12291.5
41	13377.0
42	16493.0
43	19609.0
44	22227.5
45	24846.0
46	31396.0
47	37946.0
48	42949.5
49	47953.0
50	47759.0
51	47565.0
52	40582.0
53	33599.0
54	29766.5
55	25934.0
56	23210.5
57	20487.0
58	18802.5
59	17118.0
60	16338.0
61	15558.0
62	14165.5
63	12773.0
64	11406.5
65	10040.0
66	8159.5
67	6279.0
68	5385.0
69	4491.0
70	4040.5
71	3590.0
72	2973.5
73	2357.0
74	1942.5
75	1191.0
76	854.0
77	638.0
78	422.0
79	281.0
80	140.0
81	108.0
82	76.0
83	58.0
84	40.0
85	23.0
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	412386.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.48382336605673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.65987581427025	21.978514549200888
2	12.820934639970877	8.329459524651444
3	4.705673036615461	4.585747552194974
4	2.4351823082641766	3.164161278631993
5	1.524582973743675	2.4762142012993524
6	1.1461890346821295	2.233956128803522
7	0.9288976277300335	2.1121902525032072
8	0.8246921258328271	2.143132267754511
9	0.7371900604225429	2.1552076538980716
>10	7.053223463918469	40.91566890591987
>50	0.09260870047555253	2.0597045834502494
>100	0.05974754869390486	3.5548705843694357
>500	0.0067215992280642975	1.5393198565361406
>1k	0.004481066152042864	2.7518526607863585
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	2674	0.6484216244004404	RNA PCR Primer, Index 3 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	2445	0.5928911262748978	TruSeq Adapter, Index 3 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	2365	0.5734918256196865	RNA PCR Primer, Index 24 (95% over 23bp)
TGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCTT	1442	0.3496723943101851	RNA PCR Primer, Index 24 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1324	0.32105842584374833	RNA PCR Primer, Index 24 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1093	0.2650429452018255	No Hit
ACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	862	0.20902746455990262	TruSeq Adapter, Index 3 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	840	0.2036926568797195	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	736	0.1784735660279447	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	721	0.17483619715509255	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	700	0.16974388073309957	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	684	0.1658640206020573	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	647	0.15689184404902204	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	595	0.14428229862313463	RNA PCR Primer, Index 24 (95% over 22bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	560	0.13579510458647967	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	430	0.10427124102176118	TruSeq Adapter, Index 3 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6013783203115528	0.0
2	0.0	0.0	0.0	2.3742319089396826	0.0
3	0.0	0.0	0.0	3.124984844296363	0.0
4	0.0	0.0	0.0	3.9402404543316214	0.0
5	0.0	0.0	0.0	5.9272138239416465	0.0
6	0.0	0.0	0.0	6.894026470345744	0.0
7	0.0	0.0	0.0	8.023550750995426	0.0
8	0.0	0.0	0.0	9.678796079401337	0.0
9	0.0	0.0	0.0	10.353891742202693	0.0
10	0.0	0.0	0.0	12.08770423826221	0.0
11	0.0	0.0	0.0	14.408345579141871	0.0
12	0.0	0.0	0.0	16.206903241138157	0.0
13	0.0	0.0	0.0	16.900913222078344	0.0
14	0.0	0.0	0.0	17.188507854291853	0.0
15	0.0	0.0	0.0	17.770971856464573	0.0
16	0.0	0.0	0.0	18.860970062029264	0.0
17	0.0	0.0	0.0	20.194914473333235	0.0
18	0.0	0.0	0.0	21.61736819387661	0.0
19	0.0	0.0	0.0	22.592668034317363	0.0
20	0.0	0.0	0.0	23.42465554116774	0.0
21	0.0	0.0	0.0	24.420082156038276	0.0
22	0.0	0.0	0.0	25.361675711590596	0.0
23	0.0	0.0	0.0	26.25549848927946	0.0
24	0.0021824213237112804	0.0	0.0	27.05717458885607	0.0
25	0.0026674038400915645	0.0	0.0	27.77106885296785	0.0
26	0.0026674038400915645	0.0	0.0	28.43525240915065	0.0
27	0.0026674038400915645	0.0	0.0	29.060394872764835	0.0
28	0.0026674038400915645	0.0	0.0	29.706149093325184	0.0
29	0.0026674038400915645	0.0	0.0	30.336868855877746	0.0
30	0.0026674038400915645	0.0	0.0	31.036213644498115	0.0
31	0.0026674038400915645	0.0	0.0	31.681967865058464	0.0
32	0.0026674038400915645	0.0	0.0	32.314870048934736	0.0
33	0.0026674038400915645	0.0	0.0	32.960624269495085	0.0
34	0.0026674038400915645	0.0	0.0	33.58552424185108	0.0
35	0.003152386356471849	0.0	0.0	34.20678684533423	0.0
36	0.003152386356471849	0.0	0.0	34.841628959276015	0.0
37	0.0033948776146619916	0.0	0.0	35.41318085483018	0.0
38	0.0033948776146619916	0.0	0.0	36.02134893037106	0.0
39	0.0033948776146619916	0.0	0.0	36.67534785370987	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATCA	20	7.028018E-4	45.000004	39
CACGTAG	20	7.028018E-4	45.000004	1
ACGACCG	20	7.028018E-4	45.000004	1
CTGGACG	20	7.028018E-4	45.000004	1
ATACACG	20	7.028018E-4	45.000004	27
GCGAGTT	20	7.028018E-4	45.000004	1
CATGCGG	25	3.8864397E-5	45.0	2
GCGAAAG	50	2.1827873E-11	45.0	1
ATCAATT	35	1.2093915E-7	45.0	17
TACGCGG	35	1.2093915E-7	45.0	2
CATACGG	25	3.8864397E-5	45.0	2
GCGATAC	25	3.8864397E-5	45.0	9
GTATCAA	25	3.8864397E-5	45.0	15
TACAGCG	25	3.8864397E-5	45.0	1
GTTACGG	30	2.1621036E-6	44.999996	2
TAATCGT	30	2.1621036E-6	44.999996	21
TCGGCGT	165	0.0	42.272728	4
CTTGCGG	60	3.6379788E-12	41.249996	2
TCTTAGA	165	0.0	40.90909	20
AGTGGCG	165	0.0	40.90909	33
>>END_MODULE