SRR3552519_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552519_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347188
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	2338	0.6734103713261979	RNA PCR Primer, Index 13 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	1997	0.5751926909916242	Illumina Single End Adapter 1 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT	1605	0.46228556286507594	RNA PCR Primer, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	1052	0.30300586425798126	Illumina Single End Adapter 1 (95% over 23bp)
TGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCTT	969	0.27909950804751316	Illumina Single End Adapter 1 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	792	0.22811848335771973	Illumina Single End Adapter 1 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	738	0.21256495040151155	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	695	0.2001797297141606	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	654	0.18837056580296554	No Hit
GGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	522	0.1503508185766789	RNA PCR Primer, Index 13 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	514	0.14804659147205548	Illumina Single End Adapter 1 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	505	0.14545433597935414	RNA PCR Primer, Index 13 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	464	0.13364517206815904	RNA PCR Primer, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	392	0.11290712812654816	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	370	0.10657050358883371	RNA PCR Primer, Index 13 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCG	366	0.10541839003652201	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC	362	0.10426627648421029	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	352	0.101385992603431	RNA PCR Primer, Index 13 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACC	35	1.2086457E-7	45.000004	27
CCAAGCG	45	3.8380676E-10	45.000004	20
TGCACGG	55	1.8189894E-12	45.000004	2
TCGCCTT	20	7.026477E-4	45.0	30
TATTTCG	20	7.026477E-4	45.0	19
GTACCCG	20	7.026477E-4	45.0	44
CGAACTC	20	7.026477E-4	45.0	34
CGTATCG	20	7.026477E-4	45.0	11
AGTCAAT	20	7.026477E-4	45.0	28
GTTTCGC	20	7.026477E-4	45.0	27
GCGAACT	20	7.026477E-4	45.0	33
GACGTCC	50	2.1827873E-11	45.0	22
TTATGCG	25	3.885162E-5	45.0	1
TGGAACG	20	7.026477E-4	45.0	1
TAGCGAG	20	7.026477E-4	45.0	1
ACTCTCG	20	7.026477E-4	45.0	16
GTGTCGC	20	7.026477E-4	45.0	41
TTGACAG	25	3.885162E-5	45.0	1
GTCGTCA	20	7.026477E-4	45.0	25
GTCGTAT	20	7.026477E-4	45.0	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content