##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552519_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347188
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47923891378734	34.0	31.0	34.0	31.0	34.0
2	32.63135246609906	34.0	31.0	34.0	31.0	34.0
3	32.66697869742042	34.0	31.0	34.0	31.0	34.0
4	36.083433183174535	37.0	35.0	37.0	35.0	37.0
5	36.13706694931852	37.0	35.0	37.0	35.0	37.0
6	36.17595366199293	37.0	36.0	37.0	35.0	37.0
7	36.24497102434416	37.0	37.0	37.0	35.0	37.0
8	36.241696141571715	37.0	37.0	37.0	35.0	37.0
9	37.72789382121502	39.0	38.0	39.0	35.0	39.0
10	37.80009389725451	39.0	38.0	39.0	35.0	39.0
11	37.76036038111916	39.0	37.0	39.0	35.0	39.0
12	37.594559719805986	39.0	37.0	39.0	35.0	39.0
13	37.532616334665946	39.0	37.0	39.0	35.0	39.0
14	38.7864442319435	40.0	38.0	41.0	35.0	41.0
15	38.84868428632326	40.0	38.0	41.0	35.0	41.0
16	38.90325414472851	40.0	38.0	41.0	35.0	41.0
17	38.89330852448818	40.0	38.0	41.0	35.0	41.0
18	38.889111950873875	40.0	38.0	41.0	35.0	41.0
19	38.87211827597728	40.0	38.0	41.0	35.0	41.0
20	38.82599629019436	40.0	38.0	41.0	35.0	41.0
21	38.763059207115454	40.0	38.0	41.0	35.0	41.0
22	38.76048135304215	40.0	38.0	41.0	35.0	41.0
23	38.73469705174142	40.0	38.0	41.0	35.0	41.0
24	38.700839314722856	40.0	38.0	41.0	35.0	41.0
25	38.649959099968896	40.0	38.0	41.0	35.0	41.0
26	38.62546228556287	40.0	38.0	41.0	35.0	41.0
27	38.5879494682996	40.0	38.0	41.0	35.0	41.0
28	38.54963881240135	40.0	37.0	41.0	35.0	41.0
29	38.50154959272786	40.0	37.0	41.0	35.0	41.0
30	38.40870364183094	40.0	37.0	41.0	35.0	41.0
31	38.32438909178889	40.0	37.0	41.0	34.0	41.0
32	38.21217611207761	40.0	37.0	41.0	34.0	41.0
33	38.11490316485593	40.0	37.0	41.0	34.0	41.0
34	38.035318040946116	40.0	37.0	41.0	34.0	41.0
35	37.96443137435626	40.0	37.0	41.0	34.0	41.0
36	37.96358169061143	40.0	36.0	41.0	34.0	41.0
37	37.90968293835041	40.0	36.0	41.0	34.0	41.0
38	37.838453517978735	40.0	36.0	41.0	34.0	41.0
39	37.80293385716096	40.0	36.0	41.0	34.0	41.0
40	37.704289894812035	40.0	36.0	41.0	34.0	41.0
41	37.673554385520234	40.0	35.0	41.0	34.0	41.0
42	37.66912162862772	40.0	35.0	41.0	34.0	41.0
43	37.61960378814936	40.0	35.0	41.0	34.0	41.0
44	37.54913188243833	40.0	35.0	41.0	34.0	41.0
45	37.481050612348355	40.0	35.0	41.0	33.0	41.0
46	37.394025715174486	39.0	35.0	41.0	33.0	41.0
47	37.31515778195099	39.0	35.0	41.0	33.0	41.0
48	37.298956185121604	39.0	35.0	41.0	33.0	41.0
49	37.27945090268097	39.0	35.0	41.0	33.0	41.0
50	37.1875266426259	39.0	35.0	41.0	33.0	41.0
51	36.61286680415222	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	3.0
14	5.0
15	10.0
16	13.0
17	23.0
18	41.0
19	75.0
20	136.0
21	264.0
22	368.0
23	662.0
24	1033.0
25	1406.0
26	1602.0
27	1800.0
28	2044.0
29	2461.0
30	2944.0
31	3959.0
32	5178.0
33	7609.0
34	16871.0
35	29430.0
36	20128.0
37	30801.0
38	58914.0
39	158961.0
40	446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.9527057386776	22.35705151099692	25.734760417986795	11.955482332338676
2	30.770648755141305	25.6904040462228	24.56046867979308	18.978478518842817
3	31.119739161491754	24.694401880249316	26.743147804647627	17.442711153611302
4	29.57648305817021	24.70390681705589	26.289791121812968	19.429819002960933
5	25.59535467815708	28.033226954848672	24.79002730509119	21.58139106190306
6	26.329827067755794	32.80211297625494	23.82916460246322	17.038895353526044
7	75.80993582727513	7.179971658006614	10.551919997234927	6.458172517483324
8	75.06163807504868	6.200099081765499	10.663098955033009	8.075163888152815
9	69.20746108736478	7.946703227070059	13.462158830374321	9.383676855190847
10	40.50139981796606	23.218256391349932	19.924651773678814	16.355692017005197
11	29.885249490189754	24.82487874004862	24.128714126064267	21.16115764369736
12	26.774254870560043	21.86509902415982	30.83516711407076	20.525478991209372
13	24.102791571137253	24.950459117250595	31.74360865006855	19.203140661543603
14	19.173185709183496	28.480535041533695	29.749012062628893	22.597267186653916
15	17.84508681175617	25.792366095602382	35.682396857034234	20.680150235607222
16	19.540709932370934	26.083562795949167	31.453852091662153	22.921875180017743
17	19.24692097653145	25.407848197518344	27.754415475189237	27.590815350760973
18	19.772284756385588	25.970367639434542	31.344689332580618	22.91265827159925
19	21.859338456398262	26.94851204534719	28.582209062525205	22.609940435729346
20	23.259732479233154	27.992326923741608	28.214396810949687	20.533543786075555
21	21.714460177195065	28.3238475984193	28.54678157079162	21.414910653594017
22	19.993778586817516	26.976450798990747	26.37562358146019	26.654147032731544
23	20.564650851987974	27.130257958224362	27.410797608212267	24.8942935815754
24	21.975701925181745	24.77188151664228	28.565215387628605	24.687201170547368
25	19.534085279445144	27.154740371210988	26.641761812044194	26.669412537299674
26	18.376211159371866	27.653317510973878	29.01482770141825	24.955643628236
27	21.507367766167032	26.861815500535734	27.004965609410466	24.625851123886772
28	17.169372213325346	26.537207507171907	28.938500178577602	27.354920100925145
29	19.425498577139763	26.37245526919133	28.895583948753988	25.306462204914915
30	21.219339378089106	27.63401960897266	25.068550756362544	26.07809025657569
31	20.795649619226474	29.853566367501184	22.217933799555283	27.132850213717063
32	21.949203313478577	29.746131778748115	23.66873279030381	24.635932117469498
33	20.94168001198198	29.627752111248085	21.613074184591632	27.817493692178303
34	19.784093920296787	27.761328156503108	26.229017131928522	26.22556079127159
35	20.354390128691083	26.620735739714508	26.06368883717179	26.96118529442262
36	20.75964607071673	27.83016694125373	24.04489786513359	27.365289122895952
37	20.41862045923246	28.604387248407203	25.46862218740279	25.508370104957546
38	20.494947982073114	30.642476122446627	22.112515409518764	26.750060485961498
39	21.883244812608734	28.783828934179752	24.470603822712768	24.86232243049875
40	23.161802827286657	26.547288500754636	27.526585020219592	22.764323651739115
41	20.789889051464915	25.872726015876125	25.133069115292	28.20431581736696
42	21.6565664711914	22.59525098793737	27.470419484544394	28.277763056326833
43	22.188266875583256	22.69001232761501	26.330403124531955	28.791317672269777
44	19.867334124451304	24.198416995979123	26.309089023814185	29.625159855755385
45	21.01685542127032	24.28453748401442	25.93205986382018	28.76654723089508
46	22.633846791939813	25.42426581563879	26.925469774300954	25.01641761812044
47	19.048181388757676	25.156687443114397	30.252197656601034	25.542933511526893
48	19.93329262532115	22.7945666324873	28.768851457999702	28.50328928419185
49	20.760222127492884	21.21876332131295	31.0969849188336	26.924029632360565
50	19.696245261933015	21.690841849372674	29.80978605251334	28.803126836180976
51	19.15043146652534	21.824487021440834	26.339620032950446	32.68546147908338
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	207.0
1	387.5
2	568.0
3	758.0
4	948.0
5	725.0
6	502.0
7	521.0
8	540.0
9	580.0
10	620.0
11	658.5
12	697.0
13	705.0
14	713.0
15	785.5
16	858.0
17	829.5
18	801.0
19	795.0
20	789.0
21	833.5
22	878.0
23	1002.0
24	1126.0
25	1671.5
26	2443.5
27	2670.0
28	3332.0
29	3994.0
30	4443.0
31	4892.0
32	5304.0
33	5716.0
34	5987.5
35	6259.0
36	6890.5
37	7522.0
38	8210.0
39	8898.0
40	10482.0
41	12066.0
42	14052.0
43	16038.0
44	18652.0
45	21266.0
46	28031.5
47	34797.0
48	40250.5
49	45704.0
50	44996.5
51	44289.0
52	36561.0
53	28833.0
54	24684.0
55	20535.0
56	18092.0
57	15649.0
58	14273.5
59	12898.0
60	12459.0
61	12020.0
62	11014.0
63	10008.0
64	8734.0
65	7460.0
66	6206.5
67	4953.0
68	4040.0
69	3127.0
70	2610.0
71	2093.0
72	1970.5
73	1848.0
74	1381.0
75	754.5
76	595.0
77	492.5
78	390.0
79	279.5
80	169.0
81	123.5
82	78.0
83	47.5
84	17.0
85	19.5
86	22.0
87	12.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	347188.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.991625707017334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.67711533955875	23.681322888924814
2	13.118170578056725	9.180522296563364
3	5.102884462433111	5.356746694068413
4	2.588372021650861	3.6228537988849077
5	1.661923919702761	2.907670987588907
6	1.2047716771386832	2.5294151753311396
7	0.9654688389979328	2.364832697220297
8	0.7988902503588408	2.236357489723354
9	0.7403468641990717	2.3315346328876116
>10	5.969748723139235	35.79652665241327
>50	0.0989324352711954	2.3667218781989137
>100	0.06348164596568372	4.0537756987385585
>500	0.006595495684746359	1.5546397125321727
>1k	0.0032977478423731796	2.0170793969242813
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	2338	0.6734103713261979	RNA PCR Primer, Index 13 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	1997	0.5751926909916242	Illumina Single End Adapter 1 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT	1605	0.46228556286507594	RNA PCR Primer, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	1052	0.30300586425798126	Illumina Single End Adapter 1 (95% over 23bp)
TGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCTT	969	0.27909950804751316	Illumina Single End Adapter 1 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	792	0.22811848335771973	Illumina Single End Adapter 1 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	738	0.21256495040151155	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	695	0.2001797297141606	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	654	0.18837056580296554	No Hit
GGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	522	0.1503508185766789	RNA PCR Primer, Index 13 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	514	0.14804659147205548	Illumina Single End Adapter 1 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	505	0.14545433597935414	RNA PCR Primer, Index 13 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	464	0.13364517206815904	RNA PCR Primer, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	392	0.11290712812654816	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	370	0.10657050358883371	RNA PCR Primer, Index 13 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCG	366	0.10541839003652201	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC	362	0.10426627648421029	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	352	0.101385992603431	RNA PCR Primer, Index 13 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.47841515259744	0.0
2	0.0	0.0	0.0	2.3039390762353538	0.0
3	0.0	0.0	0.0	3.259617267877922	0.0
4	0.0	0.0	0.0	4.504475961150731	0.0
5	0.0	0.0	0.0	7.422203532380151	0.0
6	0.0	0.0	0.0	8.927439888475408	0.0
7	0.0	0.0	0.0	10.542127032040279	0.0
8	0.0	0.0	0.0	12.869108379321865	0.0
9	0.0	0.0	0.0	13.83112319550215	0.0
10	0.0	0.0	0.0	16.028203739760592	0.0
11	0.0	0.0	0.0	19.201124462827057	0.0
12	0.0	0.0	0.0	21.655990414415246	0.0
13	0.0	0.0	0.0	22.627798195790177	0.0
14	0.0	0.0	0.0	23.011452008709977	0.0
15	0.0	0.0	0.0	23.69811168588776	0.0
16	0.0	0.0	0.0	25.01670564650852	0.0
17	0.0	0.0	0.0	26.56140189177045	0.0
18	0.0	0.0	0.0	28.426961761351198	0.0
19	0.0	0.0	0.0	29.477401292671406	0.0
20	0.0	0.0	0.0	30.459290067629066	0.0
21	5.760567761558579E-4	0.0	0.0	31.567911333341016	0.0
22	8.640851642337868E-4	0.0	0.0	32.6511861009021	0.0
23	8.640851642337868E-4	0.0	0.0	33.66821433920527	0.0
24	8.640851642337868E-4	0.0	0.0	34.50378469301934	0.0
25	0.002016198716545503	0.0	0.0	35.24833807620079	0.0
26	0.002016198716545503	0.0	0.0	35.91253153910849	0.0
27	0.0025922554927013605	0.0	0.0	36.54072145350646	0.0
28	0.0025922554927013605	0.0	0.0	37.210675484175724	0.0
29	0.0025922554927013605	0.0	0.0	37.84577807988755	0.0
30	0.0025922554927013605	0.0	0.0	38.53992649515536	0.0
31	0.0025922554927013605	0.0	0.0	39.2389713930205	0.0
32	0.0025922554927013605	0.0	0.0	39.93686417733332	0.0
33	0.0025922554927013605	0.0	0.0	40.54950055877507	0.0
34	0.0025922554927013605	0.0	0.0	41.184891182874985	0.0
35	0.0028802838807792898	0.0	0.0	41.82892265861723	0.0
36	0.0028802838807792898	0.0	0.0	42.46632948143369	0.0
37	0.0031683122688572186	0.0	0.0	43.092791225503184	0.0
38	0.0031683122688572186	0.0	0.0	43.7215571966773	0.0
39	0.0031683122688572186	0.0	0.0	44.35809993432953	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACC	35	1.2086457E-7	45.000004	27
CCAAGCG	45	3.8380676E-10	45.000004	20
TGCACGG	55	1.8189894E-12	45.000004	2
TCGCCTT	20	7.026477E-4	45.0	30
TATTTCG	20	7.026477E-4	45.0	19
GTACCCG	20	7.026477E-4	45.0	44
CGAACTC	20	7.026477E-4	45.0	34
CGTATCG	20	7.026477E-4	45.0	11
AGTCAAT	20	7.026477E-4	45.0	28
GTTTCGC	20	7.026477E-4	45.0	27
GCGAACT	20	7.026477E-4	45.0	33
GACGTCC	50	2.1827873E-11	45.0	22
TTATGCG	25	3.885162E-5	45.0	1
TGGAACG	20	7.026477E-4	45.0	1
TAGCGAG	20	7.026477E-4	45.0	1
ACTCTCG	20	7.026477E-4	45.0	16
GTGTCGC	20	7.026477E-4	45.0	41
TTGACAG	25	3.885162E-5	45.0	1
GTCGTCA	20	7.026477E-4	45.0	25
GTCGTAT	20	7.026477E-4	45.0	9
>>END_MODULE