FastQCFastQC Report
Sat 18 Jun 2016
SRR3552518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432271
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC28500.6593086281522471No Hit
CTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGCT26300.6084146287861086No Hit
CCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC16630.38471236793585506No Hit
TCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC13340.3086027052474027No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG10630.2459105514827504No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA9510.22000087907817087No Hit
ACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC7930.18344973407885332No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCC6980.16147277980711175No Hit
TGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGCTT6430.14874927996557716Illumina Single End Adapter 1 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4870.11266080768776994No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.10872808955493198No Hit
GCCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTG4570.1057207168651147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACCG351.2095734E-745.00000423
CGAATAT600.045.00000414
CCTTGAA302.1623473E-645.00000414
CAACGTG207.0283987E-445.020
ATTCGTA207.0283987E-445.032
TTCGTAC207.0283987E-445.033
CGACAGG253.8867533E-545.02
TCGGCGT1100.045.04
GTTAACG253.8867533E-545.01
AAATTCG207.0283987E-445.030
AATTCGT207.0283987E-445.031
CGTAAGC207.0283987E-445.025
AATAGCG207.0283987E-445.01
TACCGTT207.0283987E-445.013
GCTACGA650.044.99999610
GTAGGGT1550.043.5483864
CTACGAA700.041.78571711
TACGAAT700.041.78571712
AGGGTAC950.040.263166
TACGGGT451.9235813E-840.04