##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552518_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432271
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50443818808109	34.0	31.0	34.0	31.0	34.0
2	32.6446835434253	34.0	31.0	34.0	31.0	34.0
3	32.69346775518136	34.0	31.0	34.0	31.0	34.0
4	36.09062833268945	37.0	35.0	37.0	35.0	37.0
5	36.141256295240716	37.0	35.0	37.0	35.0	37.0
6	36.18978603699994	37.0	36.0	37.0	35.0	37.0
7	36.2361851708766	37.0	37.0	37.0	35.0	37.0
8	36.23013572504286	37.0	37.0	37.0	35.0	37.0
9	37.74284418802094	39.0	38.0	39.0	35.0	39.0
10	37.78172489017306	39.0	38.0	39.0	35.0	39.0
11	37.759819650173156	39.0	37.0	39.0	35.0	39.0
12	37.60457675856118	39.0	37.0	39.0	35.0	39.0
13	37.574040358941495	39.0	37.0	39.0	35.0	39.0
14	38.86090669973235	40.0	38.0	41.0	35.0	41.0
15	38.92486195002672	40.0	38.0	41.0	35.0	41.0
16	38.969264651110066	40.0	38.0	41.0	35.0	41.0
17	38.97837005026939	40.0	38.0	41.0	35.0	41.0
18	38.97075445727333	40.0	38.0	41.0	35.0	41.0
19	38.94586729158329	40.0	38.0	41.0	35.0	41.0
20	38.90524462663468	40.0	38.0	41.0	35.0	41.0
21	38.834532041242646	40.0	38.0	41.0	35.0	41.0
22	38.83126788519239	40.0	38.0	41.0	35.0	41.0
23	38.80915906919502	40.0	38.0	41.0	35.0	41.0
24	38.786624594293855	40.0	38.0	41.0	35.0	41.0
25	38.70796791827349	40.0	38.0	41.0	35.0	41.0
26	38.65973891378325	40.0	38.0	41.0	35.0	41.0
27	38.61845694020649	40.0	38.0	41.0	35.0	41.0
28	38.57599515118988	40.0	38.0	41.0	35.0	41.0
29	38.54238197797215	40.0	37.0	41.0	35.0	41.0
30	38.45784241829778	40.0	37.0	41.0	35.0	41.0
31	38.33661985189846	40.0	37.0	41.0	35.0	41.0
32	38.260251092485966	40.0	37.0	41.0	34.0	41.0
33	38.170603625966116	40.0	37.0	41.0	34.0	41.0
34	38.06714306534558	40.0	37.0	41.0	34.0	41.0
35	38.01294326938425	40.0	37.0	41.0	34.0	41.0
36	37.98313326593734	40.0	37.0	41.0	34.0	41.0
37	37.90226732767176	40.0	36.0	41.0	34.0	41.0
38	37.850471116498674	40.0	36.0	41.0	34.0	41.0
39	37.82757575687474	40.0	36.0	41.0	34.0	41.0
40	37.7171612252499	40.0	36.0	41.0	34.0	41.0
41	37.65958854514876	40.0	36.0	41.0	34.0	41.0
42	37.641481848192456	40.0	35.0	41.0	34.0	41.0
43	37.588901406756406	40.0	35.0	41.0	33.0	41.0
44	37.50258287046783	40.0	35.0	41.0	33.0	41.0
45	37.46564770710966	40.0	35.0	41.0	33.0	41.0
46	37.379648414998925	39.0	35.0	41.0	33.0	41.0
47	37.296092034857764	39.0	35.0	41.0	33.0	41.0
48	37.24495975904005	39.0	35.0	41.0	33.0	41.0
49	37.20670597842557	39.0	35.0	41.0	33.0	41.0
50	37.09716358488078	39.0	35.0	41.0	33.0	41.0
51	36.47120209313139	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	3.0
14	7.0
15	9.0
16	18.0
17	34.0
18	65.0
19	81.0
20	193.0
21	280.0
22	436.0
23	699.0
24	1162.0
25	1608.0
26	1839.0
27	2094.0
28	2519.0
29	3029.0
30	3740.0
31	4802.0
32	6543.0
33	9634.0
34	20340.0
35	35728.0
36	25402.0
37	40049.0
38	77324.0
39	194068.0
40	562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4581547223848	20.40710572765696	24.96535737997691	11.169382169981331
2	32.42456699616675	23.80913824892255	26.073921220715707	17.69237353419498
3	33.518093973456466	23.447790853422966	26.079473293373834	16.95464187974673
4	29.278392489896383	25.613099190091397	25.727610688665216	19.380897631347
5	25.871270568694175	29.43732056973519	24.013639591830124	20.67776926974051
6	26.84751001108101	34.17855928341249	23.20696970187683	15.766961003629667
7	77.90159413886197	7.643122022990208	8.869667407714141	5.585616430433686
8	77.76765038598472	6.649763689907489	8.723000154995361	6.8595857691124325
9	72.68357118566826	7.970925646180289	11.902487097214479	7.443016070936983
10	40.560898140286994	24.240349225370196	19.090107825877748	16.108644808465062
11	31.312995782738145	25.663299180375276	22.63186750903947	20.391837527847116
12	27.244251869776136	23.05451904013917	29.71723756624895	19.98399152383574
13	24.80480994561282	25.151351814024075	29.734819129666345	20.309019110696763
14	20.046221004878884	28.632270034307183	28.619546534465645	22.701962426348285
15	19.093115198567563	26.20508893726389	33.832942760444254	20.868853103724284
16	20.699052214929985	25.425485401518955	31.35880038216767	22.51666200138339
17	20.709693687524723	25.13839697782178	27.93201487030127	26.219894464352222
18	20.86815909464202	25.009542624881153	30.89751567882185	23.22478260165498
19	22.9439402596982	26.476909161151223	28.740304114779846	21.838846464370732
20	24.582264366566346	26.67215704962859	28.186253530771204	20.55932505303386
21	23.377001927031884	27.682171600685678	28.090942950140075	20.84988352214236
22	20.606980343349427	27.094114571646028	26.353144208147206	25.945760876857342
23	21.01343832919627	27.438574412810485	27.461939385246755	24.086047872746494
24	22.223558832306587	25.598987672085332	27.486229703126046	24.69122379248203
25	21.161956272801092	27.069361581045225	26.59743540510467	25.171246741049018
26	20.463551799681216	28.734520705760968	27.705073900400446	23.09685359415737
27	21.836764437123936	28.291742911275563	28.202447076024068	21.669045575576433
28	18.878897728508274	29.001251529711684	28.722722551362455	23.397128190417586
29	22.66356059046293	27.33748042316047	26.84519664747346	23.153762338903142
30	23.498453516428352	26.438275988905108	27.462864730689773	22.600405763976763
31	22.257102604616087	29.57589104982754	25.053033860703124	23.11397248485325
32	23.623837823957654	29.36722565242637	23.693238732184206	23.315697791431763
33	24.740267100962125	27.00158002734396	25.15921725028975	23.098935621404166
34	22.474327447365194	26.34088338102718	27.600509865339106	23.58427930626852
35	23.97361840141948	24.664157438273676	26.078547947930815	25.283676212376033
36	24.865188735769923	26.964103536901618	27.307638032623053	20.863069694705406
37	22.178448241959327	29.07042110157748	28.54297419905569	20.208156457407505
38	22.0923911157584	30.308995977060686	24.59961459362298	22.99899831355793
39	26.579159832605008	24.38447177812067	26.56088426010535	22.475484129168972
40	25.40373978360797	22.637419581697593	29.27284041723826	22.68600021745618
41	22.15623995132683	23.90791887496501	27.359226041071455	26.576615132636704
42	24.724073555709268	24.136941872112633	27.00782610908435	24.131158463093755
43	24.726618255677575	24.033765855215826	28.61468847088979	22.624927418216814
44	23.01866190422212	25.329480811805556	26.8604648472833	24.791392436689023
45	23.42997795364482	23.394120817727767	26.94744731892725	26.228453909700168
46	23.63471063291315	24.60886804805319	28.24431895732075	23.51210236171291
47	21.089316655523966	24.66924683821029	31.04811564967347	23.193320856592276
48	21.61167415810915	23.652523532691298	29.56802561356186	25.167776695637688
49	24.191537253250857	22.193022432686902	30.130172970196938	23.485267343865306
50	21.90362064538218	22.329279549171698	29.163418318601064	26.60368148684506
51	21.52376634102218	21.562630849629052	26.855606783707444	30.05799602564132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	400.0
2	542.0
3	822.5
4	1103.0
5	852.5
6	602.0
7	590.5
8	579.0
9	620.0
10	661.0
11	736.5
12	812.0
13	782.0
14	752.0
15	815.5
16	879.0
17	946.5
18	1014.0
19	1075.0
20	1136.0
21	1181.5
22	1227.0
23	1431.0
24	1635.0
25	2489.5
26	3803.5
27	4263.0
28	4884.0
29	5505.0
30	6349.0
31	7193.0
32	7660.5
33	8128.0
34	8734.5
35	9341.0
36	9874.5
37	10408.0
38	11610.0
39	12812.0
40	14129.0
41	15446.0
42	18266.0
43	21086.0
44	23795.0
45	26504.0
46	32288.5
47	38073.0
48	44478.0
49	50883.0
50	50501.0
51	50119.0
52	43035.0
53	35951.0
54	30971.5
55	25992.0
56	22789.0
57	19586.0
58	18459.5
59	17333.0
60	16696.5
61	16060.0
62	14485.5
63	12911.0
64	11380.0
65	9849.0
66	8053.0
67	6257.0
68	5433.0
69	4609.0
70	3944.0
71	3279.0
72	2866.0
73	2453.0
74	1952.0
75	1272.0
76	1093.0
77	865.5
78	638.0
79	460.5
80	283.0
81	198.5
82	114.0
83	94.0
84	74.0
85	50.5
86	27.0
87	15.0
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	432271.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.81013728405019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.40857335962868	22.790931194110136
2	13.089995871396393	8.851491149191244
3	5.081656370633732	5.154344985644841
4	2.5940877707514303	3.5082585462392624
5	1.5104122444580574	2.553362267031865
6	1.131691082261006	2.295757851262797
7	0.8812758156687202	2.0857239419010916
8	0.8223978946066752	2.2244308575012424
9	0.6676637556447979	2.0316422914141636
>10	6.658765680514951	40.286380006642844
>50	0.1048324269135693	2.504013466863112
>100	0.04248111417412612	2.788955326817005
>500	0.002740717043492008	0.7146712503721622
>1k	0.003425896304365009	2.210036865008242
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	2850	0.6593086281522471	No Hit
CTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGCT	2630	0.6084146287861086	No Hit
CCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	1663	0.38471236793585506	No Hit
TCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	1334	0.3086027052474027	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1063	0.2459105514827504	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	951	0.22000087907817087	No Hit
ACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	793	0.18344973407885332	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCC	698	0.16147277980711175	No Hit
TGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGCTT	643	0.14874927996557716	Illumina Single End Adapter 1 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	487	0.11266080768776994	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	470	0.10872808955493198	No Hit
GCCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTG	457	0.1057207168651147	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.313363607551744E-4	0.0	0.0	0.622063474070664	0.0
2	2.313363607551744E-4	0.0	0.0	2.2037101725537913	0.0
3	2.313363607551744E-4	0.0	0.0	2.81837088308029	0.0
4	2.313363607551744E-4	0.0	0.0	3.5810868644901	0.0
5	2.313363607551744E-4	0.0	0.0	5.375794351228743	0.0
6	2.313363607551744E-4	0.0	0.0	6.367764666146931	0.0
7	2.313363607551744E-4	0.0	0.0	7.397442807868212	0.0
8	2.313363607551744E-4	0.0	0.0	9.0778701323938	0.0
9	2.313363607551744E-4	0.0	0.0	9.732320696970188	0.0
10	2.313363607551744E-4	0.0	0.0	11.601749828232752	0.0
11	2.313363607551744E-4	0.0	0.0	14.447881074603663	0.0
12	2.313363607551744E-4	0.0	0.0	16.610876047664544	0.0
13	2.313363607551744E-4	0.0	0.0	17.449701691762805	0.0
14	2.313363607551744E-4	0.0	0.0	17.7980942510601	0.0
15	2.313363607551744E-4	0.0	0.0	18.47151439721841	0.0
16	2.313363607551744E-4	0.0	0.0	19.805168516971992	0.0
17	2.313363607551744E-4	0.0	0.0	21.30098942561495	0.0
18	2.313363607551744E-4	0.0	0.0	23.14381487539067	0.0
19	2.313363607551744E-4	0.0	0.0	24.123061690467324	0.0
20	2.313363607551744E-4	0.0	0.0	25.059742615165025	0.0
21	9.253454430206977E-4	0.0	0.0	26.134994019955073	0.0
22	0.0013880181645310465	0.0	0.0	27.182253725093748	0.0
23	0.0018506908860413953	0.0	0.0	28.217946612194666	0.0
24	0.0018506908860413953	0.0	0.0	29.024847838508713	0.0
25	0.00208202724679657	0.0	0.0	29.759803456627903	0.0
26	0.00208202724679657	0.0	0.0	30.417492730254864	0.0
27	0.0023133636075517443	0.0	0.0	31.035392149831935	0.0
28	0.0023133636075517443	0.0	0.0	31.674805850959235	0.0
29	0.0023133636075517443	0.0	0.0	32.34151724265565	0.0
30	0.0025446999683069187	0.0	0.0	33.086651660648066	0.0
31	0.002776036329062093	0.0	0.0	33.77001927031885	0.0
32	0.0030073726898172675	0.0	0.0	34.4272458712243	0.0
33	0.0030073726898172675	0.0	0.0	35.04560796352288	0.0
34	0.0037013817720827906	0.0	0.0	35.67530553749847	0.0
35	0.003932718132837965	0.0	0.0	36.34155425647337	0.0
36	0.003932718132837965	0.0	0.0	36.9823559757652	0.0
37	0.003932718132837965	0.0	0.0	37.616911613316645	0.0
38	0.00416405449359314	0.0	0.0	38.2607207052983	0.0
39	0.005552072658124186	0.0	0.0	38.88949293383086	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACCG	35	1.2095734E-7	45.000004	23
CGAATAT	60	0.0	45.000004	14
CCTTGAA	30	2.1623473E-6	45.000004	14
CAACGTG	20	7.0283987E-4	45.0	20
ATTCGTA	20	7.0283987E-4	45.0	32
TTCGTAC	20	7.0283987E-4	45.0	33
CGACAGG	25	3.8867533E-5	45.0	2
TCGGCGT	110	0.0	45.0	4
GTTAACG	25	3.8867533E-5	45.0	1
AAATTCG	20	7.0283987E-4	45.0	30
AATTCGT	20	7.0283987E-4	45.0	31
CGTAAGC	20	7.0283987E-4	45.0	25
AATAGCG	20	7.0283987E-4	45.0	1
TACCGTT	20	7.0283987E-4	45.0	13
GCTACGA	65	0.0	44.999996	10
GTAGGGT	155	0.0	43.548386	4
CTACGAA	70	0.0	41.785717	11
TACGAAT	70	0.0	41.785717	12
AGGGTAC	95	0.0	40.26316	6
TACGGGT	45	1.9235813E-8	40.0	4
>>END_MODULE