FastQCFastQC Report
Sat 18 Jun 2016
SRR3552517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424012
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC36870.8695508617680632TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT28300.6674339405488524TruSeq Adapter, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC19410.4577700631114213TruSeq Adapter, Index 21 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC16330.3851306095110516TruSeq Adapter, Index 21 (95% over 21bp)
TGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCTT11900.28065243436506515TruSeq Adapter, Index 21 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA9160.21603162174655435No Hit
ACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC8700.20518287218286277TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC7920.18678716640095092No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG7680.18112694923728576No Hit
GCCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG5930.1398545324188938No Hit
ACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG4890.11532692470967802TruSeq Adapter, Index 14 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4390.1035348056187089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT351.2095006E-745.0000044
CGAATGG302.162249E-645.0000042
TTAACGG302.162249E-645.0000042
CGCACGG302.162249E-645.0000042
TATCGCG302.162249E-645.0000041
TGATCCG253.8866274E-545.01
ATTAGCC253.8866274E-545.022
TTGTGCG253.8866274E-545.01
GAATGCG207.0282444E-445.01
GCAACCG253.8866274E-545.01
CGTGACC207.0282444E-445.027
ATCAGCG253.8866274E-545.01
TAGCGAG406.7975634E-945.01
CACGCAA207.0282444E-445.041
CGCGATA207.0282444E-445.018
TCTAGCG207.0282444E-445.01
TATTGCG207.0282444E-445.01
TTGAGCG453.8380676E-1045.01
CGCTACA502.1827873E-1145.028
ACGTGAC207.0282444E-445.026
Produced by FastQC (version 0.11.5)