SRR3552515_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552515_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504061
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	3334	0.6614278827364148	RNA PCR Primer, Index 40 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	2917	0.5786998002225922	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	1735	0.3442043720898859	Illumina PCR Primer Index 8 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1688	0.3348801037969611	No Hit
TCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	1449	0.28746520758400274	RNA PCR Primer, Index 40 (95% over 24bp)
TGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCTT	1205	0.23905836793562682	RNA PCR Primer, Index 40 (96% over 26bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	952	0.18886603010349937	No Hit
ACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	827	0.1640674442180609	RNA PCR Primer, Index 40 (95% over 24bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	781	0.15494156461221956	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC	738	0.14641085106762872	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	596	0.11823965750177062	No Hit
GCCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTG	541	0.10732827971217769	RNA PCR Primer, Index 40 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATA	35	1.2101191E-7	45.000004	9
ACTACGG	45	3.8380676E-10	45.000004	2
CCCGAAA	35	1.2101191E-7	45.000004	9
GTTGATC	40	6.8012014E-9	45.0	16
TTAGGCG	20	7.0295134E-4	45.0	1
CGTATGG	25	3.8876788E-5	45.0	2
CGACGTT	25	3.8876788E-5	45.0	27
ATTACGA	20	7.0295134E-4	45.0	8
AGGAACG	25	3.8876788E-5	45.0	1
GCCGATT	20	7.0295134E-4	45.0	9
TACGATA	20	7.0295134E-4	45.0	38
CGCTACA	50	2.1827873E-11	45.0	28
GTCGTTG	20	7.0295134E-4	45.0	1
ATAAGCG	20	7.0295134E-4	45.0	1
GTCCGAA	20	7.0295134E-4	45.0	39
GTATACG	20	7.0295134E-4	45.0	1
TTACGAA	20	7.0295134E-4	45.0	9
TATACGG	20	7.0295134E-4	45.0	2
CGCCGAC	20	7.0295134E-4	45.0	28
TCGACGT	25	3.8876788E-5	45.0	26