##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552515_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504061
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50262964204729	34.0	31.0	34.0	31.0	34.0
2	32.645870241895324	34.0	31.0	34.0	31.0	34.0
3	32.69866147152825	34.0	31.0	34.0	31.0	34.0
4	36.097361232073105	37.0	37.0	37.0	35.0	37.0
5	36.148450286770846	37.0	35.0	37.0	35.0	37.0
6	36.18275169076759	37.0	36.0	37.0	35.0	37.0
7	36.24611108576145	37.0	37.0	37.0	35.0	37.0
8	36.2487536230734	37.0	37.0	37.0	35.0	37.0
9	37.738835180662655	39.0	38.0	39.0	35.0	39.0
10	37.80971549078385	39.0	38.0	39.0	35.0	39.0
11	37.78705355105831	39.0	37.0	39.0	35.0	39.0
12	37.66043990707474	39.0	37.0	39.0	35.0	39.0
13	37.63097323538223	39.0	37.0	39.0	35.0	39.0
14	38.9184404268531	40.0	38.0	41.0	35.0	41.0
15	38.985210123377925	40.0	38.0	41.0	35.0	41.0
16	39.03032767859445	40.0	38.0	41.0	35.0	41.0
17	39.046950666685184	40.0	38.0	41.0	35.0	41.0
18	39.053328862974915	40.0	38.0	41.0	35.0	41.0
19	39.02199932151069	40.0	38.0	41.0	35.0	41.0
20	38.98374998264099	40.0	38.0	41.0	35.0	41.0
21	38.914696832327834	40.0	38.0	41.0	35.0	41.0
22	38.91245504016379	40.0	38.0	41.0	35.0	41.0
23	38.881811923556874	40.0	38.0	41.0	35.0	41.0
24	38.85956858396107	40.0	38.0	41.0	35.0	41.0
25	38.79174941128157	40.0	38.0	41.0	35.0	41.0
26	38.74550897609615	40.0	38.0	41.0	35.0	41.0
27	38.68634946960785	40.0	38.0	41.0	35.0	41.0
28	38.643420141609845	40.0	38.0	41.0	35.0	41.0
29	38.6321992774684	40.0	38.0	41.0	35.0	41.0
30	38.51664977056348	40.0	38.0	41.0	35.0	41.0
31	38.446233689970065	40.0	37.0	41.0	35.0	41.0
32	38.37286756959971	40.0	37.0	41.0	35.0	41.0
33	38.2806088945584	40.0	37.0	41.0	35.0	41.0
34	38.20308653119365	40.0	37.0	41.0	34.0	41.0
35	38.122334400003176	40.0	37.0	41.0	34.0	41.0
36	38.09015377107136	40.0	37.0	41.0	34.0	41.0
37	38.04896431185908	40.0	37.0	41.0	34.0	41.0
38	37.98937430191981	40.0	37.0	41.0	34.0	41.0
39	37.9486153461585	40.0	36.0	41.0	34.0	41.0
40	37.84329079218587	40.0	36.0	41.0	34.0	41.0
41	37.7879720906795	40.0	36.0	41.0	34.0	41.0
42	37.76359607269755	40.0	36.0	41.0	34.0	41.0
43	37.69869718149192	40.0	36.0	41.0	34.0	41.0
44	37.61019995595771	40.0	35.0	41.0	33.0	41.0
45	37.53850030055886	40.0	35.0	41.0	33.0	41.0
46	37.40807759378329	39.0	35.0	41.0	33.0	41.0
47	37.31937801178825	39.0	35.0	41.0	33.0	41.0
48	37.266682008725134	39.0	35.0	41.0	33.0	41.0
49	37.21225010465003	39.0	35.0	41.0	33.0	41.0
50	37.1048166789337	39.0	35.0	41.0	33.0	41.0
51	36.43299521288098	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	2.0
13	3.0
14	11.0
15	20.0
16	14.0
17	36.0
18	66.0
19	99.0
20	191.0
21	285.0
22	495.0
23	745.0
24	1237.0
25	1738.0
26	2102.0
27	2548.0
28	2897.0
29	3314.0
30	4282.0
31	5600.0
32	7475.0
33	10755.0
34	22881.0
35	38626.0
36	29706.0
37	46061.0
38	90456.0
39	231610.0
40	801.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.405857624374825	20.588579556839353	25.414384370145676	10.591178448640145
2	31.676523277936596	23.86873810907807	26.252774961760583	18.201963651224755
3	33.4965410932407	22.933137060792244	26.22103277182722	17.349289074139836
4	30.07711368266936	25.75501774586806	25.08763820251914	19.08023036894344
5	25.70244474379093	29.530156072380127	23.831044258532202	20.93635492529674
6	26.741803075421426	33.93458331432108	23.133708023433673	16.189905586823816
7	78.1320118001591	6.670224437121698	9.428422353643706	5.769341409075489
8	77.9611991405802	6.000067452153608	8.95129756120787	7.087435846058314
9	72.19562711655931	8.219044917182643	11.754529709697833	7.830798256560217
10	40.04078871406437	24.93567246821317	18.790979663175687	16.23255915454677
11	30.8428940148117	26.28590587250353	22.665312333229508	20.205887779455264
12	27.559362854892562	23.144024235162014	29.103422006463504	20.19319090348192
13	24.714667470802144	24.74819515891926	30.10488809886105	20.432249271417547
14	20.197357065910673	27.929556144990386	28.60685512269348	23.266231666405453
15	19.270286731169442	26.40434391869238	33.65981498271042	20.665554367427752
16	20.970477779475104	25.73418693372429	31.156744917777807	22.138590369022797
17	20.78200852674577	25.490764014672827	27.862699157443245	25.864528301138158
18	21.048246144811838	25.47231386677406	30.149327164767758	23.330112823646346
19	22.735740317144153	26.762038721503945	28.61776650048308	21.884454460868824
20	24.837866845481003	27.45441523942539	27.86746048593325	19.84025742916036
21	23.398556920690154	27.45679590367039	27.960901557549583	21.183745618089876
22	20.462007574480072	26.924915833599506	27.07688156790547	25.53619502401495
23	21.605321578142327	27.05704269919712	27.974590376958346	23.363045345702208
24	22.331820950242136	25.70244474379093	27.193732504597655	24.772001801369278
25	21.823945911308353	27.561743519137565	25.29693826739224	25.31737230216184
26	21.0030135241568	28.84988126437078	26.919956116422416	23.227149095050002
27	22.397884383040942	29.050849004386375	27.080254175585893	21.471012436986793
28	19.747014746231113	29.057792608434298	27.511947958679606	23.683244686654987
29	23.83044909247095	27.27249281336981	25.738551484840126	23.15850660931911
30	23.159498552754528	30.089810558642704	24.44267658081066	22.308014307792114
31	23.109306214922402	31.63644876314573	22.220326508101202	23.033918513830667
32	25.594521298017504	29.993195268033034	22.160016347227813	22.25226708672165
33	22.951388820003928	30.864518381703803	22.43736373177056	23.74672906652171
34	23.61202314799201	28.650897411226023	24.69185277178754	23.045226668994427
35	25.478067138699483	27.00923102561	23.49616415473524	24.01653768095528
36	23.308091679380073	31.680887829052434	23.078952745798624	21.932067745768865
37	23.073001085186117	31.993945177270213	24.79759394200305	20.13545979554062
38	23.419586121521007	30.960141728878053	22.58337780546402	23.036894344136922
39	24.786880952900542	27.468104058834147	24.118707854803286	23.62630713346202
40	24.63134422222707	27.37545654196615	26.403947141318213	21.589252094488565
41	22.267344626939995	27.641495771345138	24.545441920719913	25.54571768099496
42	24.046295984017807	26.289080091496864	25.085059149587053	24.579564774898277
43	23.94630808572772	25.865520244573574	26.09723823108711	24.090933438611597
44	22.627816871370726	27.24313128768145	24.859292823686022	25.2697590172618
45	23.762996938862557	25.48243168981532	25.078512322913298	25.676059048408824
46	23.62967974114244	27.489331648352085	25.70819801571635	23.172790594789124
47	21.250602605637017	27.85912816107574	28.343791723620754	22.546477509666488
48	21.911633710999265	25.40942465296859	27.048710374339613	25.630231261692533
49	23.01130220350315	24.14152255381789	28.214442299642307	24.632732943036658
50	21.784466562578736	23.94749841785022	27.48476870854916	26.78326631102188
51	21.528545156241012	23.668365535123726	25.43442162754111	29.368667681094152
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	222.0
1	367.5
2	513.0
3	806.5
4	1100.0
5	820.5
6	541.0
7	577.5
8	614.0
9	678.0
10	742.0
11	773.5
12	805.0
13	815.0
14	825.0
15	980.0
16	1135.0
17	1140.5
18	1146.0
19	1154.5
20	1163.0
21	1157.5
22	1152.0
23	1331.5
24	1511.0
25	2441.0
26	3836.5
27	4302.0
28	5864.5
29	7427.0
30	7901.0
31	8375.0
32	8871.5
33	9368.0
34	9764.0
35	10160.0
36	11655.0
37	13150.0
38	13841.5
39	14533.0
40	16803.0
41	19073.0
42	21698.5
43	24324.0
44	28043.0
45	31762.0
46	37519.5
47	43277.0
48	51654.5
49	60032.0
50	59149.5
51	58267.0
52	50516.5
53	42766.0
54	36455.0
55	30144.0
56	26769.0
57	23394.0
58	21790.5
59	20187.0
60	19721.5
61	19256.0
62	17660.0
63	16064.0
64	14204.5
65	12345.0
66	9873.5
67	7402.0
68	6134.0
69	4866.0
70	4101.5
71	3337.0
72	2780.5
73	2224.0
74	1887.5
75	1242.0
76	933.0
77	702.0
78	471.0
79	315.5
80	160.0
81	98.5
82	37.0
83	24.5
84	12.0
85	16.5
86	21.0
87	11.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504061.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.00430027760697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.91607993465985	22.085183955638644
2	13.242804012492975	8.741389602916334
3	5.218935122180963	5.167419057054298
4	2.67583894541469	3.5325676819592635
5	1.6471109370830441	2.7180871979009695
6	1.111786738520088	2.201624601766697
7	0.9204806387099005	2.126587357979351
8	0.7267339913745497	1.9188277498615582
9	0.6293282688362658	1.8693485242072816
>10	6.764577989289398	41.23418991878561
>50	0.0927317156440513	2.1332398830393373
>100	0.04636585782202563	2.940110939974282
>500	0.0036129239861318672	0.8813973245089862
>1k	0.0036129239861318672	2.4500262044073917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	3334	0.6614278827364148	RNA PCR Primer, Index 40 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	2917	0.5786998002225922	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	1735	0.3442043720898859	Illumina PCR Primer Index 8 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1688	0.3348801037969611	No Hit
TCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	1449	0.28746520758400274	RNA PCR Primer, Index 40 (95% over 24bp)
TGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCTT	1205	0.23905836793562682	RNA PCR Primer, Index 40 (96% over 26bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	952	0.18886603010349937	No Hit
ACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	827	0.1640674442180609	RNA PCR Primer, Index 40 (95% over 24bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	781	0.15494156461221956	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC	738	0.14641085106762872	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	596	0.11823965750177062	No Hit
GCCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTG	541	0.10732827971217769	RNA PCR Primer, Index 40 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.600125778427611	0.0
2	0.0	0.0	0.0	2.117402457242278	0.0
3	0.0	0.0	0.0	2.754230142780338	0.0
4	0.0	0.0	0.0	3.543817117372699	0.0
5	0.0	0.0	0.0	5.3898238506847385	0.0
6	0.0	0.0	0.0	6.376212402863939	0.0
7	0.0	0.0	0.0	7.45028875473405	0.0
8	0.0	0.0	0.0	9.18658654408891	0.0
9	0.0	0.0	0.0	9.892453492732031	0.0
10	0.0	0.0	0.0	11.662477358891087	0.0
11	0.0	0.0	0.0	14.367308718587632	0.0
12	0.0	0.0	0.0	16.422218739398605	0.0
13	0.0	0.0	0.0	17.286399860334363	0.0
14	0.0	0.0	0.0	17.62485096049883	0.0
15	0.0	0.0	0.0	18.260686702601472	0.0
16	0.0	0.0	0.0	19.52065325426883	0.0
17	0.0	0.0	0.0	20.980793991203445	0.0
18	0.0	0.0	0.0	22.70776751226538	0.0
19	0.0	0.0	0.0	23.665588093504557	0.0
20	1.9838868708350776E-4	0.0	0.0	24.55139358133242	0.0
21	9.919434354175387E-4	0.0	0.0	25.59710035094959	0.0
22	9.919434354175387E-4	0.0	0.0	26.66621698564261	0.0
23	0.0011903321225010465	0.0	0.0	27.70557531727311	0.0
24	0.0011903321225010465	0.0	0.0	28.539204580398007	0.0
25	0.003174218993336124	0.0	0.0	29.2706636696749	0.0
26	0.003174218993336124	0.0	0.0	29.923362450179642	0.0
27	0.003174218993336124	0.0	0.0	30.62069868527817	0.0
28	0.004166162428753663	0.0	0.0	31.291252447620426	0.0
29	0.004166162428753663	0.0	0.0	31.962599764711015	0.0
30	0.004166162428753663	0.0	0.0	32.74782218818754	0.0
31	0.004166162428753663	0.0	0.0	33.44972136308899	0.0
32	0.004166162428753663	0.0	0.0	34.14666082081335	0.0
33	0.004166162428753663	0.0	0.0	34.80094671081476	0.0
34	0.004166162428753663	0.0	0.0	35.42646624118906	0.0
35	0.004562939802920678	0.0	0.0	36.09642483747007	0.0
36	0.004562939802920678	0.0	0.0	36.75606722202273	0.0
37	0.004761328490004186	0.0	0.0	37.413328942330395	0.0
38	0.004959717177087694	0.0	0.0	38.07356649294431	0.0
39	0.004959717177087694	0.0	0.0	38.76078490500158	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATA	35	1.2101191E-7	45.000004	9
ACTACGG	45	3.8380676E-10	45.000004	2
CCCGAAA	35	1.2101191E-7	45.000004	9
GTTGATC	40	6.8012014E-9	45.0	16
TTAGGCG	20	7.0295134E-4	45.0	1
CGTATGG	25	3.8876788E-5	45.0	2
CGACGTT	25	3.8876788E-5	45.0	27
ATTACGA	20	7.0295134E-4	45.0	8
AGGAACG	25	3.8876788E-5	45.0	1
GCCGATT	20	7.0295134E-4	45.0	9
TACGATA	20	7.0295134E-4	45.0	38
CGCTACA	50	2.1827873E-11	45.0	28
GTCGTTG	20	7.0295134E-4	45.0	1
ATAAGCG	20	7.0295134E-4	45.0	1
GTCCGAA	20	7.0295134E-4	45.0	39
GTATACG	20	7.0295134E-4	45.0	1
TTACGAA	20	7.0295134E-4	45.0	9
TATACGG	20	7.0295134E-4	45.0	2
CGCCGAC	20	7.0295134E-4	45.0	28
TCGACGT	25	3.8876788E-5	45.0	26
>>END_MODULE