##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552512_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 628700 Sequences flagged as poor quality 0 Sequence length 51 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.504343884205504 34.0 31.0 34.0 31.0 34.0 2 32.65147606171465 34.0 31.0 34.0 31.0 34.0 3 32.693733100047716 34.0 31.0 34.0 31.0 34.0 4 36.1000970256084 37.0 35.0 37.0 35.0 37.0 5 36.14667567997455 37.0 35.0 37.0 35.0 37.0 6 36.17681883251153 37.0 36.0 37.0 35.0 37.0 7 36.262775568633685 37.0 37.0 37.0 35.0 37.0 8 36.27235247335772 37.0 37.0 37.0 35.0 37.0 9 37.814269126769524 39.0 38.0 39.0 35.0 39.0 10 37.806208048353746 39.0 38.0 39.0 35.0 39.0 11 37.7372816923811 39.0 37.0 39.0 35.0 39.0 12 37.49695403212979 39.0 37.0 39.0 35.0 39.0 13 37.39143152536981 39.0 37.0 39.0 35.0 39.0 14 38.45160648958168 40.0 38.0 41.0 34.0 41.0 15 38.572484491808495 40.0 38.0 41.0 34.0 41.0 16 38.690513758549386 40.0 38.0 41.0 35.0 41.0 17 38.72397804994433 40.0 38.0 41.0 35.0 41.0 18 38.73620645777 40.0 38.0 41.0 35.0 41.0 19 38.711210434229365 40.0 38.0 41.0 35.0 41.0 20 38.65675043741053 40.0 38.0 41.0 35.0 41.0 21 38.59976936535708 40.0 38.0 41.0 35.0 41.0 22 38.62553682201368 40.0 38.0 41.0 35.0 41.0 23 38.60547478924765 40.0 37.0 41.0 35.0 41.0 24 38.57967711149992 40.0 37.0 41.0 35.0 41.0 25 38.524619055193256 40.0 37.0 41.0 35.0 41.0 26 38.48398600286305 40.0 37.0 41.0 35.0 41.0 27 38.45458406235088 40.0 37.0 41.0 35.0 41.0 28 38.383637665023066 40.0 37.0 41.0 35.0 41.0 29 38.35621918243996 40.0 37.0 41.0 35.0 41.0 30 38.26557817719103 40.0 36.0 41.0 34.0 41.0 31 38.16465086686814 40.0 36.0 41.0 34.0 41.0 32 38.083971687609356 40.0 36.0 41.0 34.0 41.0 33 38.01919198345793 40.0 36.0 41.0 34.0 41.0 34 37.87067440750756 40.0 36.0 41.0 34.0 41.0 35 37.82369969778909 40.0 35.0 41.0 34.0 41.0 36 37.79389215842214 40.0 35.0 41.0 34.0 41.0 37 37.69133290917767 40.0 35.0 41.0 34.0 41.0 38 37.62236519802767 40.0 35.0 41.0 33.0 41.0 39 37.61427389852076 40.0 35.0 41.0 33.0 41.0 40 37.531248608239224 40.0 35.0 41.0 33.0 41.0 41 37.467074916494354 40.0 35.0 41.0 33.0 41.0 42 37.42208843645618 39.0 35.0 41.0 33.0 41.0 43 37.35085096230316 39.0 35.0 41.0 33.0 41.0 44 37.2649514871958 39.0 35.0 41.0 33.0 41.0 45 37.22422618100843 39.0 35.0 41.0 33.0 41.0 46 37.15127723874662 39.0 35.0 41.0 33.0 41.0 47 37.06690790520121 39.0 35.0 41.0 33.0 41.0 48 36.97733895339589 39.0 35.0 41.0 33.0 41.0 49 36.92573087323048 39.0 35.0 41.0 33.0 41.0 50 36.787542548115155 39.0 35.0 41.0 33.0 41.0 51 36.09771751232702 37.0 35.0 40.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 0.0 12 5.0 13 3.0 14 5.0 15 16.0 16 25.0 17 56.0 18 81.0 19 151.0 20 261.0 21 384.0 22 652.0 23 1026.0 24 1565.0 25 2167.0 26 2729.0 27 3316.0 28 3991.0 29 4742.0 30 5912.0 31 7922.0 32 10632.0 33 16583.0 34 40758.0 35 53160.0 36 37353.0 37 59136.0 38 114510.0 39 260859.0 40 697.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.164466359153806 20.945283919198346 26.277874980117705 10.612374741530141 2 32.053602672180695 24.162557658660727 25.42691267695244 18.35692699220614 3 32.20470812788293 23.366788611420393 26.477016064895814 17.95148719580086 4 29.477334181644665 24.923492921902337 25.929059965007156 19.67011293144584 5 26.305392078892954 28.02131382217274 24.621600127246698 21.05169397168761 6 27.167806585016702 32.63098457133768 23.647526642277718 16.553682201367902 7 79.29059965007158 6.113090504215047 9.022745347542548 5.573564498170828 8 79.29425799268331 4.96087163989184 8.980753936694768 6.764116430730079 9 73.80276761571497 6.810084300938445 11.331159535549547 8.05598854779704 10 43.2934627008112 21.43247972005726 18.702083664704947 16.571973914426593 11 32.98695721329728 24.14760617146493 22.499761412438364 20.365675202799427 12 28.429298552568795 21.496103069826628 29.721329728010183 20.3532686495944 13 26.16891999363766 22.98822968029267 31.122315889931606 19.720534436138063 14 21.4110068395101 25.021631938921583 31.630189279465565 21.93717194210275 15 20.282487672975982 23.338794337521872 35.320979799586446 21.057738189915696 16 24.47940194051217 24.15746779067918 29.02274534754255 22.340384921266104 17 24.70685541593765 23.819309686655004 26.763003022109118 24.710831875298233 18 25.12581517416892 22.60617146492763 30.142357245108954 22.125656115794495 19 24.602513122315887 25.236042627644345 28.179258787975186 21.982185462064578 20 24.037696834738348 27.63464291394942 27.700334022586286 20.62732622872594 21 23.153173214569748 26.365198027676158 30.3933513599491 20.088277397804994 22 21.451248608239222 24.341816446635917 29.307459837760458 24.899475107364403 23 21.374900588515985 25.565929696198502 29.45013519961826 23.609034515667247 24 23.070621918243994 25.34420232225227 27.713694926037856 23.87148083346588 25 22.78558931127724 26.230475584539526 25.893112772387468 25.09082233179577 26 21.99904564975346 25.326705901065694 27.003181167488467 25.671067281692384 27 23.580404008271035 24.960712581517416 28.05551137267377 23.403372037537775 28 19.616351200890726 26.363766502306348 27.307937012883727 26.711945283919196 29 24.19389215842214 26.191347224431365 25.82249085414347 23.79226976300302 30 25.988706855415938 25.10402417687291 25.654684269126772 23.252584698584382 31 24.03721965961508 27.738189915699063 25.29887068554159 22.925719739144267 32 26.234133927151266 28.656274852871004 22.77811356767934 22.331477652298393 33 26.32654684269127 25.48560521711468 23.113885796087164 25.073962144106886 34 22.944170510577383 25.226340066804514 28.599331954827424 23.23015746779068 35 24.262446317798634 27.29250835056466 25.021950055670434 23.42309527596628 36 27.699538730714174 27.564020995705423 23.867186257356447 20.869254016223955 37 23.589470335613168 29.880388102433592 26.07555272785112 20.45458883410212 38 22.8267854302529 30.339112454270715 23.579131541275647 23.25497057420073 39 23.756163512008907 24.48003817400986 26.17369174487037 25.590106569110866 40 24.411165897884523 23.44313663114363 28.783044377286465 23.36265309368538 41 23.34070303801495 24.96771114999205 26.008111977095595 25.68347383489741 42 25.35613170033402 24.019246063305232 27.292826467313503 23.33179576904724 43 24.94639732781931 23.185621122952124 27.654207094003496 24.213774455225067 44 23.579608716398916 23.72228407825672 26.25878797518689 26.439319230157466 45 23.40957531414029 22.717035151900745 26.287736599331957 27.58565293462701 46 23.7894067122634 24.014156195323682 27.234611102274535 24.96182599013838 47 20.621759185621123 25.332909177668206 30.670271989820264 23.37505964689041 48 21.1929378081756 25.90440591697153 28.16255765866073 24.740098616192142 49 24.687450294257992 22.378240814378877 29.236360744393192 23.69794814696994 50 21.57451884841737 22.595832670590106 28.52696039446477 27.302688086527755 51 21.23620168601877 22.55718148560522 26.265945602035945 29.940671226340065 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 182.0 1 396.0 2 610.0 3 943.0 4 1276.0 5 942.0 6 608.0 7 611.0 8 614.0 9 713.0 10 812.0 11 870.5 12 929.0 13 911.5 14 894.0 15 928.5 16 963.0 17 1140.5 18 1318.0 19 1310.0 20 1302.0 21 1528.0 22 1754.0 23 1763.5 24 1773.0 25 2414.0 26 3711.5 27 4368.0 28 5142.0 29 5916.0 30 7091.5 31 8267.0 32 9238.0 33 10209.0 34 11151.5 35 12094.0 36 13011.5 37 13929.0 38 14947.0 39 15965.0 40 18141.5 41 20318.0 42 23279.5 43 26241.0 44 30599.5 45 34958.0 46 44267.5 47 53577.0 48 63455.5 49 73334.0 50 74074.0 51 74814.0 52 65016.0 53 55218.0 54 49732.5 55 44247.0 56 39433.0 57 34619.0 58 31759.5 59 28900.0 60 26973.5 61 25047.0 62 22523.0 63 19999.0 64 18193.5 65 16388.0 66 13598.0 67 10808.0 68 9157.5 69 7507.0 70 6605.5 71 5704.0 72 4674.0 73 3644.0 74 3255.5 75 2303.0 76 1739.0 77 1361.5 78 984.0 79 772.5 80 561.0 81 399.0 82 237.0 83 166.0 84 95.0 85 61.0 86 27.0 87 27.0 88 27.0 89 14.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 628700.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.40890507009034 #Duplication Level Percentage of deduplicated Percentage of total 1 66.90874783992665 21.684392571027953 2 12.780808438379628 8.2842401479691 3 4.856413501541513 4.721731324576918 4 2.5744194304756203 3.337364597315217 5 1.7122971806784375 2.7746838390195405 6 1.2310895856430832 2.3938959308330126 7 1.0121890386611376 2.296275692686837 8 0.8439205398458832 2.1880432530051697 9 0.7176034963480554 2.0931069231998145 >10 7.2059822609394715 41.91592388574131 >50 0.09549234398443579 2.1240872562992537 >100 0.05414514349493561 3.3530747307004263 >500 0.0034456000405868117 0.733181001108481 >1k 0.0034456000405868117 2.0999988465169808 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC 3547 0.5641800540798473 No Hit CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT 2384 0.3791951646254175 No Hit CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC 1791 0.2848735485923334 No Hit TCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC 1696 0.2697630030221091 No Hit CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG 1500 0.2385875616351201 No Hit TGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCTT 1191 0.18943852393828536 Illumina Single End Adapter 1 (95% over 21bp) ACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC 1055 0.16780658501670112 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC 837 0.133131859392397 No Hit GCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG 696 0.11070462859869572 No Hit GGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG 687 0.109273103228885 No Hit AGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG 650 0.10338794337521871 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.590583744234134E-4 0.0 0.0 0.3881024335931287 0.0 2 1.590583744234134E-4 0.0 0.0 1.7022427230793702 0.0 3 1.590583744234134E-4 0.0 0.0 2.405121679656434 0.0 4 1.590583744234134E-4 0.0 0.0 3.2740575791315414 0.0 5 1.590583744234134E-4 0.0 0.0 5.3628121520598055 0.0 6 1.590583744234134E-4 0.0 0.0 6.466836328932718 0.0 7 1.590583744234134E-4 0.0 0.0 7.638937490058852 0.0 8 1.590583744234134E-4 0.0 0.0 9.39096548433275 0.0 9 1.590583744234134E-4 0.0 0.0 10.128519166534119 0.0 10 1.590583744234134E-4 0.0 0.0 11.908382376332113 0.0 11 1.590583744234134E-4 0.0 0.0 14.50835056465723 0.0 12 1.590583744234134E-4 0.0 0.0 16.372514712899633 0.0 13 1.590583744234134E-4 0.0 0.0 17.094321616033085 0.0 14 1.590583744234134E-4 0.0 0.0 17.401622395419118 0.0 15 1.590583744234134E-4 0.0 0.0 17.933513599491015 0.0 16 3.181167488468268E-4 0.0 0.0 19.05535231429935 0.0 17 3.181167488468268E-4 0.0 0.0 20.2805789724829 0.0 18 3.181167488468268E-4 0.0 0.0 21.81406076029903 0.0 19 3.181167488468268E-4 0.0 0.0 22.683314776522984 0.0 20 3.181167488468268E-4 0.0 0.0 23.55400031811675 0.0 21 9.543502465404804E-4 0.0 0.0 24.585334817878163 0.0 22 9.543502465404804E-4 0.0 0.0 25.595355495466837 0.0 23 0.0012724669953873072 0.0 0.0 26.512167965643393 0.0 24 0.001590583744234134 0.0 0.0 27.290599650071577 0.0 25 0.0017496421186575474 0.0 0.0 27.989502147288054 0.0 26 0.0017496421186575474 0.0 0.0 28.622713535867664 0.0 27 0.0019087004930809608 0.0 0.0 29.34961030698266 0.0 28 0.0019087004930809608 0.0 0.0 29.97852711945284 0.0 29 0.0019087004930809608 0.0 0.0 30.693971687609352 0.0 30 0.002067758867504374 0.0 0.0 31.434865595673614 0.0 31 0.002067758867504374 0.0 0.0 32.072371560362654 0.0 32 0.002067758867504374 0.0 0.0 32.67090822331796 0.0 33 0.002067758867504374 0.0 0.0 33.28916812470177 0.0 34 0.002067758867504374 0.0 0.0 33.927469381262924 0.0 35 0.002067758867504374 0.0 0.0 34.588675043741056 0.0 36 0.002067758867504374 0.0 0.0 35.20439001113409 0.0 37 0.002067758867504374 0.0 0.0 35.8274216637506 0.0 38 0.002067758867504374 0.0 0.0 36.541752823286146 0.0 39 0.002067758867504374 0.0 0.0 37.69588038810243 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCATT 25 3.8887814E-5 45.000004 17 TACGCAT 25 3.8887814E-5 45.000004 16 CTACGCA 25 3.8887814E-5 45.000004 15 GTCGACA 20 7.030847E-4 45.0 35 ACGTCGA 20 7.030847E-4 45.0 33 CGTCGAC 20 7.030847E-4 45.0 34 ATAACGG 20 7.030847E-4 45.0 2 GTAGTGC 20 7.030847E-4 45.0 33 CCCTCGT 55 6.002665E-11 40.909092 14 GCGTAAG 55 6.002665E-11 40.909092 1 TGCGTAG 50 1.0804797E-9 40.500004 1 CGTAAGG 95 0.0 40.263153 2 CGATTGT 45 1.925946E-8 40.0 10 ACGATGG 90 0.0 40.0 2 AAGGGTC 135 0.0 40.0 5 AACGAAG 40 3.4548975E-7 39.375 1 TATAGCG 40 3.4548975E-7 39.375 1 CCACCCG 155 0.0 39.193546 45 CGCATGG 115 0.0 39.130436 2 TCGATAG 35 6.2437757E-6 38.571426 1 >>END_MODULE