FastQCFastQC Report
Sat 18 Jun 2016
SRR3552511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527153
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC38680.733752819390196No Hit
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT28910.548417632072662No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC17120.32476339886143113No Hit
TCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC16460.31224331455953014No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG12740.24167556667608836No Hit
TGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCTT11020.20904746819234646Illumina Single End Adapter 2 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA11010.20885776994534794No Hit
ACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC9830.186473376799525No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCG9350.17736786094359702No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8790.16674475911168105No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGC8650.16408898365370206No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCC7240.13734153082691364No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTC7000.13278877289894964No Hit
GCCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTG5880.1115425692351177No Hit
ACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTG5390.10224735513219123No Hit
AGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTG5330.10110916565020023No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAACG253.887923E-545.01
ATTCGGT207.0298085E-445.013
TCGATCA502.1827873E-1145.017
TGTTGCG253.887923E-545.01
TACGCGG406.8030204E-945.02
CTACGCG253.887923E-545.01
TGTTACG207.0298085E-445.01
ACAGACG253.887923E-545.01
CTCGATC551.8189894E-1245.016
TAGGCAT253.887923E-545.040
TATCGCG207.0298085E-445.01
ATACCCG207.0298085E-445.024
TGGTCGA207.0298085E-445.045
TGCGTTT253.887923E-545.043
ACGAACG253.887923E-545.01
ATAACGG302.1632568E-644.9999962
TACGAAA800.042.187520
CATACGA800.042.187518
CCTCGAT603.6379788E-1241.24999615
ACTATGC556.002665E-1140.9090928