FastQCFastQC Report
Sat 18 Jun 2016
SRR3552507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910987
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC43710.4798092618226166Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCT25440.2792575525227034Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC18770.2060402618259097Illumina PCR Primer Index 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC17160.18836712269220088Illumina PCR Primer Index 2 (95% over 23bp)
TGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCTT14620.16048527586013853Illumina PCR Primer Index 2 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG11750.12898098436091843No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCC10410.11427166359124774No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA207.032516E-445.045
TATTGCG406.8121153E-945.01
AATCTCG207.032516E-445.01
TTACGAG253.8901664E-545.01
CGTTTCA207.032516E-445.038
GTACGAG351.2115561E-745.01
CGAATAT650.041.53846414
TCGATTG603.6379788E-1241.2499961
ACGTTAG556.002665E-1140.9090921
CGACCAA3200.040.07812529
ACGACCA3200.040.07812528
GTCGATG451.927583E-840.01
CGACAGG1350.040.02
GATTGCG451.927583E-840.01
GCGAGAC3400.039.70588321
GACACGA3400.039.70588325
CACGACC3350.039.62686527
TACGCAT403.4571713E-739.37516
ATTAGCG356.2468625E-638.5714261
ACGATAG356.2468625E-638.5714261