Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552507_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 910987 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC | 4371 | 0.4798092618226166 | Illumina PCR Primer Index 2 (95% over 23bp) |
CTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCT | 2544 | 0.2792575525227034 | Illumina PCR Primer Index 2 (95% over 24bp) |
CCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC | 1877 | 0.2060402618259097 | Illumina PCR Primer Index 2 (95% over 23bp) |
TCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC | 1716 | 0.18836712269220088 | Illumina PCR Primer Index 2 (95% over 23bp) |
TGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCTT | 1462 | 0.16048527586013853 | Illumina PCR Primer Index 2 (96% over 25bp) |
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG | 1175 | 0.12898098436091843 | No Hit |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCC | 1041 | 0.11427166359124774 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCGA | 20 | 7.032516E-4 | 45.0 | 45 |
TATTGCG | 40 | 6.8121153E-9 | 45.0 | 1 |
AATCTCG | 20 | 7.032516E-4 | 45.0 | 1 |
TTACGAG | 25 | 3.8901664E-5 | 45.0 | 1 |
CGTTTCA | 20 | 7.032516E-4 | 45.0 | 38 |
GTACGAG | 35 | 1.2115561E-7 | 45.0 | 1 |
CGAATAT | 65 | 0.0 | 41.538464 | 14 |
TCGATTG | 60 | 3.6379788E-12 | 41.249996 | 1 |
ACGTTAG | 55 | 6.002665E-11 | 40.909092 | 1 |
CGACCAA | 320 | 0.0 | 40.078125 | 29 |
ACGACCA | 320 | 0.0 | 40.078125 | 28 |
GTCGATG | 45 | 1.927583E-8 | 40.0 | 1 |
CGACAGG | 135 | 0.0 | 40.0 | 2 |
GATTGCG | 45 | 1.927583E-8 | 40.0 | 1 |
GCGAGAC | 340 | 0.0 | 39.705883 | 21 |
GACACGA | 340 | 0.0 | 39.705883 | 25 |
CACGACC | 335 | 0.0 | 39.626865 | 27 |
TACGCAT | 40 | 3.4571713E-7 | 39.375 | 16 |
ATTAGCG | 35 | 6.2468625E-6 | 38.571426 | 1 |
ACGATAG | 35 | 6.2468625E-6 | 38.571426 | 1 |