##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552507_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	910987
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55905956945598	34.0	31.0	34.0	31.0	34.0
2	32.70447328007974	34.0	31.0	34.0	31.0	34.0
3	32.74668024900465	34.0	31.0	34.0	31.0	34.0
4	36.15236331583217	37.0	37.0	37.0	35.0	37.0
5	36.18621121925999	37.0	35.0	37.0	35.0	37.0
6	36.22331383433573	37.0	37.0	37.0	35.0	37.0
7	36.29375940600689	37.0	37.0	37.0	35.0	37.0
8	36.30274745962346	37.0	37.0	37.0	35.0	37.0
9	37.819659336521816	39.0	39.0	39.0	35.0	39.0
10	37.83247620438053	39.0	38.0	39.0	35.0	39.0
11	37.773394131859185	39.0	37.0	39.0	35.0	39.0
12	37.63641522875738	39.0	37.0	39.0	35.0	39.0
13	37.61468604930696	39.0	37.0	39.0	35.0	39.0
14	38.908346661368384	40.0	38.0	41.0	35.0	41.0
15	38.9668315793749	40.0	38.0	41.0	35.0	41.0
16	39.0162142818723	40.0	38.0	41.0	35.0	41.0
17	39.00657638363665	40.0	38.0	41.0	35.0	41.0
18	39.00053019417401	40.0	38.0	41.0	35.0	41.0
19	38.981539802434064	40.0	38.0	41.0	35.0	41.0
20	38.93753807683315	40.0	38.0	41.0	35.0	41.0
21	38.884404497539485	40.0	38.0	41.0	35.0	41.0
22	38.87716070591567	40.0	38.0	41.0	35.0	41.0
23	38.83943459127298	40.0	38.0	41.0	35.0	41.0
24	38.81965384796929	40.0	38.0	41.0	35.0	41.0
25	38.75020170430533	40.0	38.0	41.0	35.0	41.0
26	38.74302377531183	40.0	38.0	41.0	35.0	41.0
27	38.7198994058093	40.0	38.0	41.0	35.0	41.0
28	38.669334469097805	40.0	38.0	41.0	35.0	41.0
29	38.647115710762066	40.0	38.0	41.0	35.0	41.0
30	38.54358294904318	40.0	38.0	41.0	35.0	41.0
31	38.47521973420038	40.0	38.0	41.0	35.0	41.0
32	38.41087084667509	40.0	37.0	41.0	35.0	41.0
33	38.301795744615454	40.0	37.0	41.0	34.0	41.0
34	38.21214792307684	40.0	37.0	41.0	34.0	41.0
35	38.16437995273259	40.0	37.0	41.0	34.0	41.0
36	38.12894695533526	40.0	37.0	41.0	34.0	41.0
37	38.10860967280543	40.0	37.0	41.0	34.0	41.0
38	38.030533915412626	40.0	37.0	41.0	34.0	41.0
39	37.95873047584653	40.0	36.0	41.0	34.0	41.0
40	37.897577023601876	40.0	36.0	41.0	34.0	41.0
41	37.888269536228286	40.0	36.0	41.0	34.0	41.0
42	37.86683125006174	40.0	36.0	41.0	34.0	41.0
43	37.80566352758053	40.0	36.0	41.0	34.0	41.0
44	37.708776305260116	40.0	35.0	41.0	34.0	41.0
45	37.629792741279516	40.0	35.0	41.0	34.0	41.0
46	37.521862551276804	40.0	35.0	41.0	33.0	41.0
47	37.46929978144584	39.0	35.0	41.0	33.0	41.0
48	37.42180843414889	39.0	35.0	41.0	33.0	41.0
49	37.35063727583379	39.0	35.0	41.0	33.0	41.0
50	37.262796285786735	39.0	35.0	41.0	33.0	41.0
51	36.63012315214158	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	2.0
12	4.0
13	5.0
14	12.0
15	25.0
16	24.0
17	51.0
18	98.0
19	147.0
20	290.0
21	512.0
22	749.0
23	1335.0
24	2239.0
25	3072.0
26	3496.0
27	4239.0
28	4839.0
29	6027.0
30	7466.0
31	9752.0
32	13189.0
33	19035.0
34	42436.0
35	68066.0
36	52534.0
37	83184.0
38	163430.0
39	423411.0
40	1315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69524482786253	20.28272631771913	25.774352433130222	10.24767642128812
2	32.34217392783871	23.81340238664218	25.82660345317771	18.017820232341407
3	32.930327216524496	23.84754118335388	25.610683796805006	17.611447803316622
4	29.20349028032233	25.691475289987675	25.620892504503356	19.484141925186638
5	25.875012486456995	29.57056467326098	24.379272152072424	20.1751506882096
6	27.534750770318343	33.29641367000846	23.78310557669868	15.38572998297451
7	80.3613004356813	6.4757235833222655	8.042485787393234	5.120490193603202
8	81.00027772075782	5.165935408518453	7.973330025565678	5.860456845158054
9	74.76670907488253	7.2880293571697505	10.765027382388553	7.18023418555918
10	40.87259203479303	24.201443050230136	18.24241180170518	16.68355311327165
11	30.651589978781256	26.527930694949543	23.22590772425951	19.59457160200969
12	27.079200910660635	23.46641609594868	29.228518079840875	20.22586491354981
13	24.62285411317615	25.076757407076062	30.286381693701447	20.01400678604634
14	21.217756126047902	26.7832581584589	29.679677097477793	22.319308618015405
15	20.426855707051804	26.24768520297216	32.64316614836436	20.682292941611678
16	22.64302344599868	25.814638408671037	30.003172383360027	21.53916576197026
17	22.86629776275622	25.34734304660769	28.090631370151275	23.695727820484816
18	23.34292366411376	24.218018478858642	30.516022731389143	21.923035125638457
19	23.6273404560109	26.96481947601887	27.75692737657069	21.650912691399547
20	24.029870898267482	28.267801845690443	27.5498991752901	20.152428080751974
21	23.3712445951479	27.94452610190925	28.19151096557909	20.492718337363762
22	22.101632624834384	26.211241214199543	27.113339707372337	24.57378645359374
23	21.495367112812808	26.857902472812455	27.969005046175194	23.677725368199546
24	22.0780318489726	25.69389025309911	28.66945411954287	23.55862377838542
25	21.339382450023987	27.126402462384203	26.76909769294183	24.76511739464998
26	21.202388178975113	27.285790027739143	27.901495850105434	23.610325943180307
27	22.12226958233213	26.50850121900752	27.595893245457948	23.773335953202405
28	19.911919709062808	27.001922091094606	27.995350098299976	25.09080810154261
29	23.004279973259774	25.616721204583598	27.183373637604046	24.19562518455258
30	23.723938980468436	25.852619192150932	26.796211142420255	23.62723068496038
31	22.664209258749025	28.43234865041982	25.1799421945648	23.723499896266357
32	24.532622309648765	26.36590862438213	26.016507370577184	23.08496169539192
33	22.78956779844279	25.416828121586803	26.054488154057083	25.739115925913325
34	22.147846237103273	25.4250609503758	29.1234671844933	23.30362562802762
35	23.072886879834726	23.761480679746253	28.402381153627875	24.763251286791142
36	21.756292899898682	26.890614245867393	27.283155522526663	24.06993733170726
37	21.611834197414453	27.162846451156824	28.516872359320168	22.70844699210856
38	21.89789755506939	26.889516535362194	27.94562381241445	23.266962097153964
39	22.335335191391316	24.12570102537138	30.004160322814705	23.534803460422594
40	23.063995424742615	22.116012632452495	32.47466758581626	22.34532435698863
41	20.122570355010556	23.647428558256046	29.602068964760196	26.627932121973203
42	21.48636588667017	23.886180593136892	29.07835128272961	25.549102237463323
43	22.386927585135684	23.63743939265873	28.018950874161764	25.956682148043825
44	22.128087448009687	24.040738232268957	27.787224186514187	26.043950133207172
45	22.902192896276237	22.69143247927797	28.359460672874587	26.04691395157121
46	22.56256126596757	23.79276542914443	29.641037687694777	24.00363561719322
47	19.620916654134472	24.316702653276064	31.92603187531765	24.136348817271816
48	20.816542936397557	23.289135849358992	30.630074852879353	25.2642463613641
49	22.17078838666194	21.828961335342875	31.110103656803005	24.89014662119218
50	20.89140679285215	21.871332960843567	30.471675226979087	26.765585019325194
51	20.60797794040969	21.304255713857607	28.708532613527964	29.37923373220474
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	199.0
1	441.5
2	684.0
3	1296.0
4	1908.0
5	1404.5
6	901.0
7	992.0
8	1083.0
9	1178.0
10	1273.0
11	1334.5
12	1396.0
13	1485.5
14	1575.0
15	1611.0
16	1647.0
17	1706.0
18	1765.0
19	1759.0
20	1753.0
21	2049.0
22	2345.0
23	2832.5
24	3320.0
25	4594.5
26	6785.0
27	7701.0
28	9273.5
29	10846.0
30	12604.0
31	14362.0
32	15812.5
33	17263.0
34	19503.0
35	21743.0
36	24515.5
37	27288.0
38	28768.5
39	30249.0
40	32917.5
41	35586.0
42	40441.0
43	45296.0
44	50772.5
45	56249.0
46	67782.0
47	79315.0
48	90892.5
49	102470.0
50	102533.0
51	102596.0
52	89501.5
53	76407.0
54	66158.5
55	55910.0
56	49351.5
57	42793.0
58	39426.5
59	36060.0
60	34581.0
61	33102.0
62	29797.0
63	26492.0
64	23848.0
65	21204.0
66	17183.0
67	13162.0
68	11370.0
69	9578.0
70	7911.5
71	6245.0
72	5595.5
73	4946.0
74	4342.0
75	3008.5
76	2279.0
77	1756.0
78	1233.0
79	943.5
80	654.0
81	479.5
82	305.0
83	224.5
84	144.0
85	92.0
86	40.0
87	26.5
88	13.0
89	6.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	910987.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.180262998125162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.42477067957029	20.047170486995842
2	13.022543024640603	7.860475467761074
3	5.1116970064200515	4.628170800614587
4	2.5811217085049885	3.1159572797140282
5	1.571316542037675	2.371137324600081
6	1.1515244748547708	2.085198689993697
7	0.8699892361989752	1.837955276781617
8	0.7177816473958113	1.7330271114906501
9	0.6474539727375738	1.758629805876084
>10	7.729911319377036	47.02194492399312
>50	0.112403923868635	2.2422748518510893
>100	0.052552459341674934	2.841667620732572
>500	0.004379371611804789	0.893913736816322
>1k	0.00255463344021946	1.562476622779244
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC	4371	0.4798092618226166	Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCT	2544	0.2792575525227034	Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC	1877	0.2060402618259097	Illumina PCR Primer Index 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC	1716	0.18836712269220088	Illumina PCR Primer Index 2 (95% over 23bp)
TGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCTT	1462	0.16048527586013853	Illumina PCR Primer Index 2 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1175	0.12898098436091843	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCC	1041	0.11427166359124774	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1954210103986116E-4	0.0	0.0	0.28760015236221814	0.0
2	2.1954210103986116E-4	0.0	0.0	1.277515485950952	0.0
3	2.1954210103986116E-4	0.0	0.0	1.790036520828508	0.0
4	2.1954210103986116E-4	0.0	0.0	2.4566761106360464	0.0
5	2.1954210103986116E-4	0.0	0.0	4.098741255363688	0.0
6	2.1954210103986116E-4	0.0	0.0	5.061652910524519	0.0
7	2.1954210103986116E-4	0.0	0.0	6.094488724866546	0.0
8	2.1954210103986116E-4	0.0	0.0	7.77947435034748	0.0
9	2.1954210103986116E-4	0.0	0.0	8.500999465414983	0.0
10	2.1954210103986116E-4	0.0	0.0	10.261507573653631	0.0
11	2.1954210103986116E-4	0.0	0.0	12.626963941307615	0.0
12	2.1954210103986116E-4	0.0	0.0	14.290214898785603	0.0
13	2.1954210103986116E-4	0.0	0.0	14.946645780894787	0.0
14	2.1954210103986116E-4	0.0	0.0	15.211303783698341	0.0
15	2.1954210103986116E-4	0.0	0.0	15.70110221111827	0.0
16	2.1954210103986116E-4	0.0	0.0	16.774772856253712	0.0
17	2.1954210103986116E-4	0.0	0.0	18.004867248380055	0.0
18	2.1954210103986116E-4	0.0	0.0	19.40346020305449	0.0
19	2.1954210103986116E-4	0.0	0.0	20.25660080769539	0.0
20	2.1954210103986116E-4	0.0	0.0	21.089763081141662	0.0
21	2.1954210103986116E-4	0.0	0.0	22.084947425155352	0.0
22	2.1954210103986116E-4	0.0	0.0	23.063775882641576	0.0
23	2.1954210103986116E-4	0.0	0.0	24.01746676955873	0.0
24	7.68397353639514E-4	0.0	0.0	24.84107896160977	0.0
25	9.879394546793753E-4	0.0	0.0	25.5539321636862	0.0
26	0.0014270236567590975	0.0	0.0	26.212009611553185	0.0
27	0.001536794707279028	0.0	0.0	26.845608115154224	0.0
28	0.001536794707279028	0.0	0.0	27.484804942331778	0.0
29	0.001536794707279028	0.0	0.0	28.15890896357467	0.0
30	0.001536794707279028	0.0	0.0	28.866054071024067	0.0
31	0.001536794707279028	0.0	0.0	29.525668313598327	0.0
32	0.001536794707279028	0.0	0.0	30.20306546635682	0.0
33	0.001536794707279028	0.0	0.0	30.843469775090096	0.0
34	0.001536794707279028	0.0	0.0	31.50066905455292	0.0
35	0.0016465657577989588	0.0	0.0	32.14645214476167	0.0
36	0.0016465657577989588	0.0	0.0	32.77565980634191	0.0
37	0.0016465657577989588	0.0	0.0	33.411124417801794	0.0
38	0.0016465657577989588	0.0	0.0	34.04450337930179	0.0
39	0.0016465657577989588	0.0	0.0	34.7550513893173	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	20	7.032516E-4	45.0	45
TATTGCG	40	6.8121153E-9	45.0	1
AATCTCG	20	7.032516E-4	45.0	1
TTACGAG	25	3.8901664E-5	45.0	1
CGTTTCA	20	7.032516E-4	45.0	38
GTACGAG	35	1.2115561E-7	45.0	1
CGAATAT	65	0.0	41.538464	14
TCGATTG	60	3.6379788E-12	41.249996	1
ACGTTAG	55	6.002665E-11	40.909092	1
CGACCAA	320	0.0	40.078125	29
ACGACCA	320	0.0	40.078125	28
GTCGATG	45	1.927583E-8	40.0	1
CGACAGG	135	0.0	40.0	2
GATTGCG	45	1.927583E-8	40.0	1
GCGAGAC	340	0.0	39.705883	21
GACACGA	340	0.0	39.705883	25
CACGACC	335	0.0	39.626865	27
TACGCAT	40	3.4571713E-7	39.375	16
ATTAGCG	35	6.2468625E-6	38.571426	1
ACGATAG	35	6.2468625E-6	38.571426	1
>>END_MODULE