FastQCFastQC Report
Sat 18 Jun 2016
SRR3552506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065906
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC36080.33849138667011913No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT30520.28632918850255085No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG21060.1975783980951416No Hit
TCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC16760.15723712972813736No Hit
CCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC16500.15479789024548132No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA15380.1442903970894244No Hit
TGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCTT13670.1282477066458018Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCC13330.12505793193771308No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11600.10882760768773231No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG207.033055E-445.00000412
GAACCGT207.033055E-445.00000411
TAAACGG700.045.02
CCGGTAC253.8906146E-545.011
GCGTAAT253.8906146E-545.09
TATAGCG600.044.9999961
CGAATAT1400.043.39285714
TTAATCG750.042.020
TACGAAT1500.042.012
ACGTAGG1300.041.5384642
AACGGGC1500.040.54
TCGTAAG451.9281288E-840.01
GTATACG451.9281288E-840.01
CGTTAAC403.4578989E-739.37500411
GCTACGA1600.039.37500410
CGTTGAT700.038.57142625
GTTCGTA356.247863E-638.57142630
AGTTCGT356.247863E-638.57142629
GGCGATA3250.038.0769238
TGCGCTA301.13986636E-437.49999630