FastQCFastQC Report
Sat 18 Jun 2016
SRR3552504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741732
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC37480.5053038024515593TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT24000.32356700263707106TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC16510.22258713389741847TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC14690.19804996953077392TruSeq Adapter, Index 15 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG12740.1717601505665119No Hit
TGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCTT12340.1663673671892274TruSeq Adapter, Index 15 (96% over 25bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA9530.12848306396380363No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC8280.11163061590978952No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCC8220.11082169840319685No Hit
ACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC8110.10933868297444359TruSeq Adapter, Index 14 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7770.10475481710375176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG302.1644555E-645.0000041
GCGTTAG551.8189894E-1245.01
AAACGTT207.031666E-445.037
CTAACGG207.031666E-445.02
GGGCGTA253.889463E-545.07
GGTTACG253.889463E-545.01
TACCGCG207.031666E-445.01
CGTGTAC1250.044.99999618
CGCACGG750.042.02
TACGAAT501.0804797E-940.512
TAAGTAG403.456007E-739.3751
GCGTACG403.456007E-739.3751
TCGGCGT1450.038.7931064
TCGTGTA1450.038.79310617
CCCTCGT1650.038.18181614
TTGGCAG1300.038.076921
TTTACGG601.546141E-1037.5000042
GCGAATT301.1395312E-437.5000047
TAAACGC601.546141E-1037.50000420
TAACCGC301.1395312E-437.50000421
Produced by FastQC (version 0.11.5)