##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552504_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	741732
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57654516725718	34.0	31.0	34.0	31.0	34.0
2	32.71942022185911	34.0	31.0	34.0	31.0	34.0
3	32.76600173647625	34.0	31.0	34.0	31.0	34.0
4	36.1595131395167	37.0	37.0	37.0	35.0	37.0
5	36.19707926852286	37.0	37.0	37.0	35.0	37.0
6	36.24673736605674	37.0	37.0	37.0	35.0	37.0
7	36.304471156698106	37.0	37.0	37.0	35.0	37.0
8	36.28987019570411	37.0	37.0	37.0	35.0	37.0
9	37.799695847017524	39.0	39.0	39.0	35.0	39.0
10	37.848605695857806	39.0	38.0	39.0	35.0	39.0
11	37.83023652747893	39.0	38.0	39.0	35.0	39.0
12	37.69385842865078	39.0	37.0	39.0	35.0	39.0
13	37.66071303381815	39.0	37.0	39.0	35.0	39.0
14	38.97764556470531	40.0	38.0	41.0	35.0	41.0
15	39.041136151601926	40.0	38.0	41.0	35.0	41.0
16	39.081033311222924	40.0	38.0	41.0	35.0	41.0
17	39.06921367825576	40.0	38.0	41.0	35.0	41.0
18	39.06186870729589	40.0	38.0	41.0	35.0	41.0
19	39.0455096989209	40.0	38.0	41.0	35.0	41.0
20	38.99648794982554	40.0	38.0	41.0	35.0	41.0
21	38.93992708956874	40.0	38.0	41.0	35.0	41.0
22	38.93542816003624	40.0	38.0	41.0	35.0	41.0
23	38.90058943122314	40.0	38.0	41.0	35.0	41.0
24	38.88038536830014	40.0	38.0	41.0	35.0	41.0
25	38.82477094152605	40.0	38.0	41.0	35.0	41.0
26	38.814276585073856	40.0	38.0	41.0	35.0	41.0
27	38.785520915910325	40.0	38.0	41.0	35.0	41.0
28	38.7459877691673	40.0	38.0	41.0	35.0	41.0
29	38.71849670770575	40.0	38.0	41.0	35.0	41.0
30	38.625351205017445	40.0	38.0	41.0	35.0	41.0
31	38.58099690993512	40.0	38.0	41.0	35.0	41.0
32	38.467588023706675	40.0	38.0	41.0	35.0	41.0
33	38.38935221886072	40.0	37.0	41.0	35.0	41.0
34	38.34955078114467	40.0	37.0	41.0	35.0	41.0
35	38.243060835989276	40.0	37.0	41.0	34.0	41.0
36	38.210074528266276	40.0	37.0	41.0	34.0	41.0
37	38.192453608581	40.0	37.0	41.0	34.0	41.0
38	38.108106431972736	40.0	37.0	41.0	34.0	41.0
39	38.082523067630895	40.0	37.0	41.0	34.0	41.0
40	37.99204968910604	40.0	36.0	41.0	34.0	41.0
41	37.91349301364914	40.0	36.0	41.0	34.0	41.0
42	37.88204634558034	40.0	36.0	41.0	34.0	41.0
43	37.85832888428705	40.0	36.0	41.0	34.0	41.0
44	37.81521088479397	40.0	36.0	41.0	34.0	41.0
45	37.75554917409523	40.0	35.0	41.0	34.0	41.0
46	37.647253994704286	40.0	35.0	41.0	34.0	41.0
47	37.573604752120715	40.0	35.0	41.0	34.0	41.0
48	37.50963016291598	39.0	35.0	41.0	34.0	41.0
49	37.46800596441842	39.0	35.0	41.0	34.0	41.0
50	37.39893115033462	39.0	35.0	41.0	34.0	41.0
51	36.78073616885883	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	3.0
13	6.0
14	8.0
15	23.0
16	25.0
17	55.0
18	72.0
19	127.0
20	231.0
21	378.0
22	641.0
23	1050.0
24	1580.0
25	2225.0
26	2857.0
27	3391.0
28	3871.0
29	4599.0
30	5579.0
31	7504.0
32	10106.0
33	14510.0
34	32161.0
35	57953.0
36	41446.0
37	65186.0
38	127659.0
39	357352.0
40	1127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02061661085136	19.98484627870983	25.81997271251611	10.1745643979227
2	31.346901576310582	24.838351318265897	25.66789622127669	18.146850884146833
3	32.97727481084812	24.500763078847886	25.705915344086545	16.81604676621745
4	29.016275420232645	25.70969029245064	25.812153176619045	19.461881110697664
5	25.47645241138309	29.8204742413702	24.41879277151316	20.284280575733554
6	27.596625196162496	34.10126568625865	22.561383356791943	15.740725760786914
7	79.6210760759951	6.696623578327482	8.348702765958596	5.333597579718821
8	79.7092480842137	6.06607238193849	8.171145373261501	6.053534160586303
9	73.64614173313272	7.697389353567057	10.811452114780002	7.84501679852022
10	39.72539947042868	25.53846942022186	18.180286141086	16.555844968263468
11	30.841867413027884	26.141247782217835	22.88036649355832	20.136518311195957
12	26.68942421251881	24.052892419364408	28.764027977760158	20.493655390356626
13	24.75260606256707	25.443691252366087	30.040364983578975	19.763337701487867
14	20.16038137764044	28.09855850900325	28.992816812541456	22.74824330081485
15	19.837488472925532	25.4198281859216	33.28061887582038	21.462064465332492
16	22.061472337717667	26.244519583892835	29.715180145928716	21.97882793246078
17	21.803697292283466	25.474969395954332	27.662282333780936	25.05905097798127
18	23.00547906791132	24.899694229182508	29.728122826034202	22.36670387687197
19	23.903916778566924	26.308828525666954	27.57316658847131	22.21408810729482
20	24.458564548920634	27.416506231361193	27.86235459707819	20.262574622639985
21	23.255839036201756	28.767937745708693	27.608624139176953	20.367599078912598
22	22.08209973413578	26.136259457593848	26.715714031483067	25.0659267767873
23	21.20792954867796	28.15653093030906	27.538113496518957	23.097426024494023
24	21.69422378972459	25.30927612668727	29.053485625535906	23.943014458052232
25	21.222085605043333	27.45910922004174	26.921448717326474	24.397356457588455
26	20.403056629618245	27.65999040084559	27.63019527268609	24.306757696850077
27	22.200875788020472	27.12799232067647	27.24407198287252	23.42705990843054
28	19.579443788322468	26.77395609195774	28.675721149957127	24.970878969762662
29	21.44534683686291	25.875248742133277	26.397674631807718	26.281729789196095
30	22.51931964644912	27.97587268717003	26.06035603155857	23.44445163482228
31	24.864371498061296	27.996500083588145	23.275522695528846	23.86360572282172
32	22.245096611714203	27.738320579400643	24.74734809877422	25.269234710110926
33	21.90144149099675	29.356155592586003	23.55055464777035	25.1918482686469
34	22.846122319112563	25.478744344318432	26.692390243376312	24.98274309319269
35	20.6291490727109	25.86271052078109	27.084715234073762	26.42342517243425
36	22.096525429670017	31.005133929775177	24.686948924948634	22.211391715606176
37	23.20703434663733	28.186460878052987	26.48301542875324	22.12348934655644
38	21.32279583461412	29.233334951168345	25.27449267390378	24.169376540313753
39	23.202315661182208	26.98818441162037	25.536851585208677	24.272648341988752
40	23.943823375558825	24.899424590013645	28.28312652009081	22.873625514336716
41	20.698311519524573	27.109791676778134	26.690502769194264	25.50139403450303
42	21.65782250192792	26.09298237099114	27.843345035673263	24.40585009140768
43	22.394611530849417	25.43317532478038	27.374712160187237	24.797500984182967
44	22.716156239720007	24.782805649479865	25.96449930702733	26.536538803772793
45	23.03163406729115	23.887603608850636	25.98458742510772	27.09617489875049
46	23.036487572330707	25.84275722228514	26.31300793278435	24.807747272599805
47	20.807110924161286	26.024359202515196	29.403612086306108	23.764917787017414
48	20.5296522194	25.237552107769385	28.589975894258302	25.642819778572314
49	22.467953384780486	23.847427372689868	29.29616087751371	24.388458365015936
50	20.849848732426267	23.54206101395113	28.87808534618973	26.730004907432875
51	21.222085605043333	23.02731984058932	26.593297848818708	29.157296705548635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	193.0
1	405.5
2	618.0
3	1030.5
4	1443.0
5	1119.0
6	795.0
7	776.5
8	758.0
9	839.5
10	921.0
11	1010.5
12	1100.0
13	1179.5
14	1259.0
15	1228.0
16	1197.0
17	1197.5
18	1198.0
19	1274.0
20	1350.0
21	1685.5
22	2021.0
23	2081.5
24	2142.0
25	3725.5
26	6141.5
27	6974.0
28	8209.0
29	9444.0
30	10536.5
31	11629.0
32	12706.5
33	13784.0
34	15003.5
35	16223.0
36	17739.5
37	19256.0
38	21285.5
39	23315.0
40	26528.5
41	29742.0
42	32697.0
43	35652.0
44	40350.5
45	45049.0
46	55462.5
47	65876.0
48	75574.5
49	85273.0
50	84418.0
51	83563.0
52	73872.0
53	64181.0
54	56498.0
55	48815.0
56	43207.5
57	37600.0
58	33819.0
59	30038.0
60	28238.0
61	26438.0
62	23837.5
63	21237.0
64	18766.0
65	16295.0
66	13422.5
67	10550.0
68	8727.0
69	6904.0
70	5934.5
71	4965.0
72	4196.5
73	3428.0
74	2889.5
75	1924.0
76	1497.0
77	1118.5
78	740.0
79	525.0
80	310.0
81	244.0
82	178.0
83	125.5
84	73.0
85	49.5
86	26.0
87	23.5
88	21.0
89	11.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	741732.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.276882978854925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.42939179621631	19.4484552997433
2	13.123369843027533	7.684227263651016
3	5.180930366951198	4.550444762244784
4	2.7098845610929625	3.173478927252972
5	1.5854015788197962	2.320780824879951
6	1.0673664767487228	1.8749498061195018
7	0.8133167312779185	1.666796513645789
8	0.664618980837688	1.5566377702008654
9	0.6008410598717375	1.583167805888042
>10	7.578966169743411	46.70115720929482
>50	0.16854672542981927	3.1211530548979516
>100	0.06907652677046483	3.572945013518251
>500	0.005526122141637186	1.158129793823696
>1k	0.002763061070818593	1.5876759548390973
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	3748	0.5053038024515593	TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	2400	0.32356700263707106	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	1651	0.22258713389741847	TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	1469	0.19804996953077392	TruSeq Adapter, Index 15 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1274	0.1717601505665119	No Hit
TGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCTT	1234	0.1663673671892274	TruSeq Adapter, Index 15 (96% over 25bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	953	0.12848306396380363	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	828	0.11163061590978952	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCC	822	0.11082169840319685	No Hit
ACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	811	0.10933868297444359	TruSeq Adapter, Index 14 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	777	0.10475481710375176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3467559711593945	0.0
2	0.0	0.0	0.0	1.4547033160224987	0.0
3	0.0	0.0	0.0	1.9862969374383201	0.0
4	0.0	0.0	0.0	2.683449008536776	0.0
5	0.0	0.0	0.0	4.447563270830974	0.0
6	0.0	0.0	0.0	5.408557268663075	0.0
7	0.0	0.0	0.0	6.460824125155717	0.0
8	0.0	0.0	0.0	8.182065759600503	0.0
9	0.0	0.0	0.0	8.876656258594748	0.0
10	0.0	0.0	0.0	10.620412763639697	0.0
11	0.0	0.0	0.0	12.875270313266787	0.0
12	0.0	0.0	0.0	14.66270836366774	0.0
13	0.0	0.0	0.0	15.335592909568415	0.0
14	0.0	0.0	0.0	15.600917851730815	0.0
15	1.3481958443211295E-4	0.0	0.0	16.12698387018492	0.0
16	1.3481958443211295E-4	0.0	0.0	17.18491314922371	0.0
17	1.3481958443211295E-4	0.0	0.0	18.420669460128455	0.0
18	1.3481958443211295E-4	0.0	0.0	19.837218833756666	0.0
19	1.3481958443211295E-4	0.0	0.0	20.67121278305372	0.0
20	1.3481958443211295E-4	0.0	0.0	21.494286346011766	0.0
21	6.740979221605647E-4	0.0	0.0	22.49761369335555	0.0
22	9.437370910247906E-4	0.0	0.0	23.49285186563341	0.0
23	0.0017526545976174683	0.0	0.0	24.44103800294446	0.0
24	0.0018874741820495812	0.0	0.0	25.216655072182405	0.0
25	0.0018874741820495812	0.0	0.0	25.925536447126454	0.0
26	0.0018874741820495812	0.0	0.0	26.58493903458392	0.0
27	0.0018874741820495812	0.0	0.0	27.232342679026925	0.0
28	0.0018874741820495812	0.0	0.0	27.87192678757287	0.0
29	0.0018874741820495812	0.0	0.0	28.495197726402527	0.0
30	0.002022293766481694	0.0	0.0	29.237514358285743	0.0
31	0.002022293766481694	0.0	0.0	29.91633096590143	0.0
32	0.002157113350913807	0.0	0.0	30.56252123408455	0.0
33	0.002157113350913807	0.0	0.0	31.19064567795376	0.0
34	0.002157113350913807	0.0	0.0	31.821196874342753	0.0
35	0.00229193293534592	0.0	0.0	32.469948714630085	0.0
36	0.002426752519778033	0.0	0.0	33.09092772052439	0.0
37	0.002426752519778033	0.0	0.0	33.66782072230941	0.0
38	0.002561572104210146	0.0	0.0	34.252937718744775	0.0
39	0.002561572104210146	0.0	0.0	34.884028193471494	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCG	30	2.1644555E-6	45.000004	1
GCGTTAG	55	1.8189894E-12	45.0	1
AAACGTT	20	7.031666E-4	45.0	37
CTAACGG	20	7.031666E-4	45.0	2
GGGCGTA	25	3.889463E-5	45.0	7
GGTTACG	25	3.889463E-5	45.0	1
TACCGCG	20	7.031666E-4	45.0	1
CGTGTAC	125	0.0	44.999996	18
CGCACGG	75	0.0	42.0	2
TACGAAT	50	1.0804797E-9	40.5	12
TAAGTAG	40	3.456007E-7	39.375	1
GCGTACG	40	3.456007E-7	39.375	1
TCGGCGT	145	0.0	38.793106	4
TCGTGTA	145	0.0	38.793106	17
CCCTCGT	165	0.0	38.181816	14
TTGGCAG	130	0.0	38.07692	1
TTTACGG	60	1.546141E-10	37.500004	2
GCGAATT	30	1.1395312E-4	37.500004	7
TAAACGC	60	1.546141E-10	37.500004	20
TAACCGC	30	1.1395312E-4	37.500004	21
>>END_MODULE