FastQCFastQC Report
Sat 18 Jun 2016
SRR3552503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758759
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT26250.3459596525378941Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC22520.29680043333917616No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC21200.2794036050972707No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC18710.24658686091367615No Hit
ACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC11950.15749401325058418No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG11400.15024533481645688No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10830.14273306807563402No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG10010.13192594750111697No Hit
TCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC8990.1184829438596445No Hit
TGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCTT8960.11808756139960119Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCG253.8895487E-545.01
CGGATCA207.0317695E-445.039
AATTACG207.0317695E-445.01
GCGTTAG650.041.538461
CGAATAT556.002665E-1140.9090914
TTAATCG403.4561526E-739.37520
ATATGCG356.2454837E-638.5714261
TATCGCG356.2454837E-638.5714261
CGTAAGG1350.038.3333362
CACAACG3900.038.07692312
ACAACGG659.094947E-1238.076922
ACAACGA3850.037.98701513
CGTTAGG950.037.8947372
GACAACG301.1395561E-437.5000041
TACGTTG301.1395561E-437.5000041
GCGTAAG850.037.0588231
CGTTTTT10250.036.658541
AACGAGC3950.036.45569615
CGTTCAG1550.036.2903238
TGTAGCG751.8189894E-1236.0000041
Produced by FastQC (version 0.11.5)