##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552503_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	758759
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64406484799521	34.0	31.0	34.0	31.0	34.0
2	32.79177841712586	34.0	31.0	34.0	31.0	34.0
3	32.82613056319595	34.0	31.0	34.0	31.0	34.0
4	36.21161660026438	37.0	37.0	37.0	35.0	37.0
5	36.242095316167585	37.0	37.0	37.0	35.0	37.0
6	36.276137745977316	37.0	37.0	37.0	35.0	37.0
7	36.358345667069514	37.0	37.0	37.0	35.0	37.0
8	36.36579599055827	37.0	37.0	37.0	35.0	37.0
9	37.937445223055015	39.0	39.0	39.0	37.0	39.0
10	37.90944819105935	39.0	38.0	39.0	35.0	39.0
11	37.84789636762134	39.0	38.0	39.0	35.0	39.0
12	37.63418555826026	39.0	37.0	39.0	35.0	39.0
13	37.5535697105405	39.0	37.0	39.0	35.0	39.0
14	38.67959127997164	40.0	38.0	41.0	35.0	41.0
15	38.79616057272467	40.0	38.0	41.0	35.0	41.0
16	38.91500858638909	40.0	38.0	41.0	35.0	41.0
17	38.939957219617824	40.0	38.0	41.0	35.0	41.0
18	38.94509060189072	40.0	38.0	41.0	35.0	41.0
19	38.91165310724486	40.0	38.0	41.0	35.0	41.0
20	38.85246435297638	40.0	38.0	41.0	35.0	41.0
21	38.79314248661301	40.0	38.0	41.0	35.0	41.0
22	38.81892142300783	40.0	38.0	41.0	35.0	41.0
23	38.788772192488	40.0	38.0	41.0	35.0	41.0
24	38.76586373275309	40.0	38.0	41.0	35.0	41.0
25	38.70918961093048	40.0	38.0	41.0	35.0	41.0
26	38.68204133328237	40.0	38.0	41.0	35.0	41.0
27	38.65790850586286	40.0	38.0	41.0	35.0	41.0
28	38.61519665664592	40.0	37.0	41.0	35.0	41.0
29	38.57858555878744	40.0	37.0	41.0	35.0	41.0
30	38.48187500906085	40.0	37.0	41.0	35.0	41.0
31	38.39770335508376	40.0	37.0	41.0	34.0	41.0
32	38.3192054394083	40.0	37.0	41.0	34.0	41.0
33	38.213843921455954	40.0	37.0	41.0	34.0	41.0
34	38.11655743127923	40.0	36.0	41.0	34.0	41.0
35	38.05470775305466	40.0	36.0	41.0	34.0	41.0
36	37.98188621156388	40.0	36.0	41.0	34.0	41.0
37	37.94638350253506	40.0	36.0	41.0	34.0	41.0
38	37.86039177130024	40.0	35.0	41.0	34.0	41.0
39	37.800366124158	40.0	35.0	41.0	34.0	41.0
40	37.73573295341472	40.0	35.0	41.0	34.0	41.0
41	37.67462791215656	40.0	35.0	41.0	34.0	41.0
42	37.66361255681976	40.0	35.0	41.0	34.0	41.0
43	37.61646978816726	40.0	35.0	41.0	34.0	41.0
44	37.545662061339634	40.0	35.0	41.0	34.0	41.0
45	37.45546214278842	39.0	35.0	41.0	34.0	41.0
46	37.36211761573833	39.0	35.0	41.0	33.0	41.0
47	37.29438069268371	39.0	35.0	41.0	33.0	41.0
48	37.22322898311585	39.0	35.0	41.0	33.0	41.0
49	37.17661339107674	39.0	35.0	41.0	33.0	41.0
50	37.09739060755787	39.0	35.0	41.0	33.0	41.0
51	36.47220790791279	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	7.0
12	4.0
13	1.0
14	8.0
15	22.0
16	23.0
17	45.0
18	88.0
19	138.0
20	216.0
21	389.0
22	584.0
23	1053.0
24	1628.0
25	2342.0
26	2990.0
27	3744.0
28	4316.0
29	5157.0
30	6429.0
31	8148.0
32	11071.0
33	17569.0
34	46057.0
35	58478.0
36	42455.0
37	67002.0
38	130769.0
39	346943.0
40	1082.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.773528880711794	19.99831303483715	26.023282755130417	11.20487532932064
2	32.07961948392045	23.768811967963476	25.882790187661698	18.268778360454373
3	31.84304897866121	23.74627516774101	27.23486640685646	17.175809446741326
4	29.12400380094338	25.046819872976794	26.583407906858437	19.24576841922139
5	26.257480965629405	28.413633314398908	25.698673755434857	19.63021196453683
6	27.73871545510498	32.08396869098093	23.966634992138477	16.210680861775607
7	80.67436432384987	5.685599775422763	8.439702197931096	5.200333702796277
8	81.80515815957372	4.9313418358134795	7.838061887898529	5.4254381167142665
9	76.17438475194363	6.466480134008296	10.410420172940288	6.948714941107783
10	42.951055605271236	22.634064307639186	18.040642681009388	16.37423740608019
11	32.911504179851576	24.26778463253813	23.135672855280795	19.6850383323295
12	28.344046001431284	22.750833927505308	29.12110432956973	19.784015741493675
13	26.619519504875726	23.259691153581045	30.875548098935234	19.245241242607996
14	21.929756352148704	24.39747007943234	32.052733476637506	21.620040091781448
15	20.62433526323905	24.00564606152942	33.79360244820819	21.576416227023337
16	25.136439897253275	24.640498498205623	29.651312208487806	20.571749396053292
17	25.2178886840222	23.914971684026153	27.676903997184876	23.190235634766772
18	26.789402168541	22.477888235922077	29.89539498048788	20.837314615049046
19	25.125896364985458	24.9372989315448	28.360388476446406	21.576416227023337
20	24.73288619970241	27.774431670662224	28.05950242435345	19.433179705281916
21	23.393066836768988	27.502672126459128	29.684787923438137	19.419473113333748
22	22.18952262839716	24.776378270307173	29.374280898150793	23.659818203144873
23	21.332465249176614	26.133330873175804	29.88933244943388	22.6448714282137
24	22.2401315832827	25.581904135568738	28.913792126353695	23.264172154794867
25	22.708528004280673	26.329045190897244	26.802845172182472	24.15958163263961
26	21.44198619060861	26.046214937812927	28.24203732674011	24.269761544838346
27	22.854951308650044	24.858486027842833	29.05678878273602	23.229773880771102
28	19.429489468988177	26.611216473214817	28.571390915956187	25.387903141840823
29	22.864045105231042	25.927204817339895	27.913869884904162	23.2948801925249
30	25.62592338278689	24.656313796607353	28.407438989191565	21.31032383141419
31	24.70573660411277	26.515665712037684	26.240215931540845	22.538381752308702
32	25.876727656607695	25.539861800650797	26.48153102632061	22.10187951642089
33	25.529186474229633	25.225005568302976	25.74664682725345	23.499161130213942
34	21.83789582726531	25.34151159986241	29.535728736001815	23.284863836870468
35	23.67510632493321	25.31699788733972	28.611060956113864	22.396834831613198
36	24.773215210626827	28.552148969567416	26.347759960672622	20.326875859133136
37	23.961758608464613	28.501671808835216	25.58164054726204	21.95492903543813
38	24.677005478682954	27.822536536634164	25.889116307022388	21.611341677660494
39	23.86449452329396	24.17381540120117	27.419773604003378	24.54191647150149
40	25.355218191810575	22.40171121528707	28.7891148572867	23.453955735615654
41	23.116826291352062	24.617302727216416	26.054649763627186	26.211221217804336
42	23.377515126673952	25.38078625756004	28.23861067875307	23.00308793701294
43	23.187467957546467	24.585935718719647	28.980216379640964	23.246379944092922
44	23.39412118999577	23.657182320077915	27.68705214699266	25.26164434293366
45	23.964394491531564	21.603697616766325	27.776408582962443	26.655499308739667
46	23.348125030477398	23.54832034941266	28.21726002591073	24.886294594199214
47	21.215563835157145	25.62144238157307	30.29209538206466	22.870898401205125
48	22.237627494369093	25.863943623732965	28.687765153362264	23.210663728535675
49	24.479709631121345	23.336527144982792	29.220477121193948	22.963286102701915
50	21.91644514266058	23.182723368025947	28.201049345048958	26.699782144264518
51	21.822212323016927	22.527706425887533	26.848841331700847	28.801239919394696
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	195.0
1	413.5
2	632.0
3	1415.5
4	2199.0
5	1566.5
6	934.0
7	969.0
8	1004.0
9	1099.0
10	1194.0
11	1220.0
12	1246.0
13	1256.0
14	1266.0
15	1341.5
16	1417.0
17	1538.5
18	1660.0
19	1671.5
20	1683.0
21	2036.0
22	2389.0
23	2719.0
24	3049.0
25	4084.5
26	5726.0
27	6332.0
28	7449.5
29	8567.0
30	9846.5
31	11126.0
32	12220.0
33	13314.0
34	14791.5
35	16269.0
36	17931.0
37	19593.0
38	21353.5
39	23114.0
40	24919.5
41	26725.0
42	30622.0
43	34519.0
44	37907.0
45	41295.0
46	50348.0
47	59401.0
48	69765.5
49	80130.0
50	82645.5
51	85161.0
52	76760.0
53	68359.0
54	60534.0
55	52709.0
56	47362.0
57	42015.0
58	39166.0
59	36317.0
60	32529.0
61	28741.0
62	26324.0
63	23907.0
64	21633.5
65	19360.0
66	16360.0
67	13360.0
68	10968.0
69	8576.0
70	7230.5
71	5885.0
72	4982.5
73	4080.0
74	3484.5
75	2314.5
76	1740.0
77	1260.0
78	780.0
79	544.0
80	308.0
81	220.0
82	132.0
83	95.0
84	58.0
85	32.5
86	7.0
87	4.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	758759.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.729393822457524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.89072428847354	24.256944249947534
2	11.805723585666927	8.436226947027379
3	4.479893892633357	4.801916795181582
4	2.5326672691712893	3.619626651258762
5	1.8608436430003483	3.3243407681388004
6	1.4911780681354125	3.1967333073492936
7	1.2837302340991794	3.2106832167147616
8	1.1239883568016413	3.2127538121618193
9	0.9878447487125966	3.1765584655979056
>10	6.427752960154431	35.96534486642654
>50	0.07094367026131387	1.7348681251170346
>100	0.03953630801030596	2.754718241882064
>500	0.002216989234222565	0.5551418674932198
>1k	0.0029559856456300862	1.7541426857033087
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT	2625	0.3459596525378941	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	2252	0.29680043333917616	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	2120	0.2794036050972707	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC	1871	0.24658686091367615	No Hit
ACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	1195	0.15749401325058418	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1140	0.15024533481645688	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1083	0.14273306807563402	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1001	0.13192594750111697	No Hit
TCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	899	0.1184829438596445	No Hit
TGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCTT	896	0.11808756139960119	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3542626841988036	0.0
2	0.0	0.0	0.0	1.2279261267411654	0.0
3	0.0	0.0	0.0	1.6383331202661187	0.0
4	0.0	0.0	0.0	2.1576020844563293	0.0
5	0.0	0.0	0.0	3.494390181862752	0.0
6	0.0	0.0	0.0	4.328910760860826	0.0
7	0.0	0.0	0.0	5.297070611353539	0.0
8	0.0	0.0	0.0	6.791616310317242	0.0
9	0.0	0.0	0.0	7.479186408332554	0.0
10	0.0	0.0	0.0	9.169973601631085	0.0
11	0.0	0.0	0.0	11.72071764552381	0.0
12	0.0	0.0	0.0	13.460136881407667	0.0
13	0.0	0.0	0.0	14.143753154822546	0.0
14	0.0	0.0	0.0	14.436863351867984	0.0
15	0.0	0.0	0.0	14.948092872703981	0.0
16	0.0	0.0	0.0	15.912694281056304	0.0
17	0.0	0.0	0.0	17.051000383520986	0.0
18	0.0	0.0	0.0	18.267724007227592	0.0
19	0.0	0.0	0.0	19.05651201501399	0.0
20	0.0	0.0	0.0	19.861510703662166	0.0
21	0.0	0.0	0.0	20.837973585815785	0.0
22	0.0	0.0	0.0	21.77476642781173	0.0
23	0.0	0.0	0.0	22.667935405049562	0.0
24	0.0	0.0	0.0	23.377251538367254	0.0
25	0.0	0.0	0.0	24.05401451580805	0.0
26	0.0	0.0	0.0	24.68201365651017	0.0
27	0.0	0.0	0.0	25.405563558389424	0.0
28	0.0	0.0	0.0	26.049905174106666	0.0
29	0.0	0.0	0.0	26.694378583977258	0.0
30	0.0	0.0	0.0	27.435457108251764	0.0
31	0.0	0.0	0.0	28.052253745919323	0.0
32	0.0	0.0	0.0	28.66048376361928	0.0
33	0.0	0.0	0.0	29.28136602004062	0.0
34	1.3179415334776918E-4	0.0	0.0	29.918195369017038	0.0
35	1.3179415334776918E-4	0.0	0.0	30.579406636362798	0.0
36	2.6358830669553836E-4	0.0	0.0	31.173402885501194	0.0
37	2.6358830669553836E-4	0.0	0.0	31.82393882642578	0.0
38	2.6358830669553836E-4	0.0	0.0	32.524424751469176	0.0
39	2.6358830669553836E-4	0.0	0.0	33.580754890551546	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTCG	25	3.8895487E-5	45.0	1
CGGATCA	20	7.0317695E-4	45.0	39
AATTACG	20	7.0317695E-4	45.0	1
GCGTTAG	65	0.0	41.53846	1
CGAATAT	55	6.002665E-11	40.90909	14
TTAATCG	40	3.4561526E-7	39.375	20
ATATGCG	35	6.2454837E-6	38.571426	1
TATCGCG	35	6.2454837E-6	38.571426	1
CGTAAGG	135	0.0	38.333336	2
CACAACG	390	0.0	38.076923	12
ACAACGG	65	9.094947E-12	38.07692	2
ACAACGA	385	0.0	37.987015	13
CGTTAGG	95	0.0	37.894737	2
GACAACG	30	1.1395561E-4	37.500004	1
TACGTTG	30	1.1395561E-4	37.500004	1
GCGTAAG	85	0.0	37.058823	1
CGTTTTT	1025	0.0	36.65854	1
AACGAGC	395	0.0	36.455696	15
CGTTCAG	155	0.0	36.29032	38
TGTAGCG	75	1.8189894E-12	36.000004	1
>>END_MODULE