SRR3552502_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552502_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	583692
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	3014	0.5163682215963213	RNA PCR Primer, Index 23 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	2620	0.44886686814278765	RNA PCR Primer, Index 30 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	2217	0.37982360560021383	RNA PCR Primer, Index 30 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1667	0.2855958279366515	RNA PCR Primer, Index 23 (96% over 25bp)
TGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCTT	1394	0.23882458556910152	RNA PCR Primer, Index 23 (96% over 27bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1061	0.18177394927461746	No Hit
ACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1040	0.17817616140019052	RNA PCR Primer, Index 23 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	906	0.15521884829670443	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCC	904	0.1548762018324733	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	669	0.11461524228531486	No Hit
GCCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTG	590	0.10108070694818501	RNA PCR Primer, Index 23 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCCG	30	2.1636588E-6	45.000004	12
AACCGTA	20	7.030431E-4	45.0	1
GGTACGT	20	7.030431E-4	45.0	39
TAACGCG	20	7.030431E-4	45.0	1
TATTGCG	20	7.030431E-4	45.0	1
GTATGCG	25	3.8884384E-5	45.0	1
GTCGTTT	20	7.030431E-4	45.0	9
CAATGCG	35	1.2105556E-7	45.0	1
CGGTATG	20	7.030431E-4	45.0	2
GTACGTG	20	7.030431E-4	45.0	40
TGCGTCG	25	3.8884384E-5	45.0	1
CTACGAA	140	0.0	40.17857	11
GTCGATG	45	1.9255822E-8	40.0	1
CAACCGG	45	1.9255822E-8	40.0	2
GTGGCGT	130	0.0	39.807693	34
AAGGGCG	210	0.0	39.642857	5
TGGCGTT	125	0.0	39.600002	35
AACGGGA	155	0.0	39.193546	4
TCGATGG	75	0.0	39.0	2
TTGTGCG	70	0.0	38.571426	1