##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552502_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	583692
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58142993222453	34.0	31.0	34.0	31.0	34.0
2	32.73050512941757	34.0	31.0	34.0	31.0	34.0
3	32.773087518759894	34.0	31.0	34.0	31.0	34.0
4	36.16913029474449	37.0	37.0	37.0	35.0	37.0
5	36.199322587940216	37.0	37.0	37.0	35.0	37.0
6	36.23293449284897	37.0	37.0	37.0	35.0	37.0
7	36.28585795248179	37.0	37.0	37.0	35.0	37.0
8	36.28288035470762	37.0	37.0	37.0	35.0	37.0
9	37.776572233301124	39.0	38.0	39.0	35.0	39.0
10	37.83930566120488	39.0	38.0	39.0	35.0	39.0
11	37.79972485488922	39.0	37.0	39.0	35.0	39.0
12	37.686480198460835	39.0	37.0	39.0	35.0	39.0
13	37.66045962596712	39.0	37.0	39.0	35.0	39.0
14	38.97714548083578	40.0	38.0	41.0	35.0	41.0
15	39.03387916915085	40.0	38.0	41.0	35.0	41.0
16	39.06358319113505	40.0	38.0	41.0	35.0	41.0
17	39.054400265893655	40.0	38.0	41.0	35.0	41.0
18	39.04180458186852	40.0	38.0	41.0	35.0	41.0
19	39.04041857692071	40.0	38.0	41.0	35.0	41.0
20	39.00641948150737	40.0	38.0	41.0	35.0	41.0
21	38.93000075382222	40.0	38.0	41.0	35.0	41.0
22	38.91914914030002	40.0	38.0	41.0	35.0	41.0
23	38.90042522426211	40.0	38.0	41.0	35.0	41.0
24	38.87878024711663	40.0	38.0	41.0	35.0	41.0
25	38.82501045071716	40.0	38.0	41.0	35.0	41.0
26	38.79509570115746	40.0	38.0	41.0	35.0	41.0
27	38.76433975452807	40.0	38.0	41.0	35.0	41.0
28	38.68876222391261	40.0	38.0	41.0	35.0	41.0
29	38.66334470919595	40.0	38.0	41.0	35.0	41.0
30	38.59061799716289	40.0	38.0	41.0	35.0	41.0
31	38.490549810516505	40.0	38.0	41.0	35.0	41.0
32	38.43775826977242	40.0	38.0	41.0	35.0	41.0
33	38.36700177490869	40.0	37.0	41.0	35.0	41.0
34	38.26041131281566	40.0	37.0	41.0	34.0	41.0
35	38.216233904182346	40.0	37.0	41.0	34.0	41.0
36	38.17206848817527	40.0	37.0	41.0	34.0	41.0
37	38.10427074553018	40.0	37.0	41.0	34.0	41.0
38	38.05232382832042	40.0	37.0	41.0	34.0	41.0
39	38.037540346621164	40.0	37.0	41.0	34.0	41.0
40	37.97620320305915	40.0	36.0	41.0	34.0	41.0
41	37.934643613412554	40.0	36.0	41.0	34.0	41.0
42	37.9060360601139	40.0	36.0	41.0	34.0	41.0
43	37.84577996614653	40.0	36.0	41.0	34.0	41.0
44	37.75997786503841	40.0	35.0	41.0	34.0	41.0
45	37.70857404247445	40.0	35.0	41.0	34.0	41.0
46	37.61894972005784	40.0	35.0	41.0	34.0	41.0
47	37.554561309731845	40.0	35.0	41.0	33.0	41.0
48	37.495543882732676	39.0	35.0	41.0	33.0	41.0
49	37.44009511865847	39.0	35.0	41.0	33.0	41.0
50	37.35999294148284	39.0	35.0	41.0	33.0	41.0
51	36.747680283437155	39.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	6.0
14	13.0
15	12.0
16	18.0
17	47.0
18	63.0
19	111.0
20	163.0
21	278.0
22	447.0
23	796.0
24	1232.0
25	1753.0
26	2167.0
27	2673.0
28	3154.0
29	3801.0
30	4619.0
31	6063.0
32	7883.0
33	11535.0
34	26017.0
35	45610.0
36	32435.0
37	52267.0
38	102706.0
39	276950.0
40	866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.589057242518315	21.21307127731749	26.110859837037342	11.087011643126853
2	30.809228154574676	25.104507171590495	26.27824263481425	17.808022039020578
3	31.452375567936514	24.296204162469248	27.22309025993161	17.028330009662632
4	29.316831479615963	25.47233815094262	26.019373231087627	19.19145713835379
5	25.662678261823018	28.99440115677446	24.943977303098208	20.39894327830431
6	27.061189805582398	33.03077650541724	23.81752705193835	16.090506637062013
7	78.50099024828164	6.763841203922618	8.982305736587104	5.752862811208651
8	78.55204457145206	5.891463305990145	8.862550797338322	6.693941325219466
9	72.61689384127246	7.76334094008484	11.503327097167684	8.116438121475024
10	40.028131274713374	24.159488223241024	19.07855512838963	16.733825373655968
11	30.270416589571212	25.881458029234594	23.547178991659983	20.300946389534204
12	27.699882814909234	22.79712588145803	29.2974719543869	20.205519349245833
13	24.529032434914306	24.51104349554217	30.261850427965438	20.698073641578095
14	19.7484289659615	27.73928715829581	28.618689308745022	23.893594566997663
15	19.56048738033072	26.115314241072348	33.30335176771311	21.020846610883822
16	21.30969758023067	25.967976261452957	30.581882225557315	22.14044393275906
17	21.416431953838668	25.22957313103486	28.001240380200514	25.352754534925953
18	21.710765266613212	25.387704474277527	30.270245266339096	22.63128499277016
19	23.496124668489546	26.755377836256105	27.428335492006056	22.32016200324829
20	25.101252030180298	27.046969977316802	27.302070269936884	20.54970772256601
21	23.664021435962802	27.53250001713232	27.078322128793953	21.72515641811092
22	21.425854731605025	27.4112031687945	25.395756666186962	25.767185433413513
23	21.665021963638356	27.31800333052363	27.281340158850902	23.735634546987107
24	22.7681722552305	25.089430727164324	27.869321491471528	24.273075526133645
25	21.87780541792589	27.0270964823914	25.845137504026095	25.249960595656614
26	20.838730015144975	27.454376623287622	26.79118439176826	24.91570896979914
27	23.954585637630803	26.26864853381578	26.723854361546845	23.052911467006574
28	20.320648561227497	26.457618058839248	27.996100683237053	25.225632696696206
29	23.7531095166629	24.925817040493957	25.650856958807044	25.6702164840361
30	25.899446968606732	25.28336862591915	25.344873666248642	23.47231073922548
31	23.735120577290765	27.967832349937982	24.522008182397567	23.77503889037369
32	25.612480554813153	26.404165210419194	24.936439080885126	23.046915153882527
33	26.265736038869814	26.956511310759783	23.995189243642194	22.782563406728208
34	23.11099004269375	26.1742494329201	26.930470179478217	23.78429034490793
35	24.033051677939735	24.272047586740953	26.82921814929792	24.865682586021396
36	24.647759434770393	28.197062834508614	25.762902352610624	21.392275378110373
37	23.912782768994607	27.882513380344427	28.430576399882128	19.774127450778835
38	24.41287528353995	26.742871240311672	27.09117137120262	21.753082104945758
39	25.799565524283352	25.713561261761342	26.316790362040255	22.17008285191505
40	24.528175818753724	24.61075361663343	29.00570849009409	21.85536207451875
41	20.516299692303473	26.88781069468144	27.565394077698514	25.03049553531657
42	21.959355276412904	25.751252372826766	28.42218156150847	23.867210789251867
43	24.496652344044463	24.540168445001818	27.530101491882704	23.433077719071015
44	23.284026507130474	23.99073483960719	27.08037800757934	25.644860645683
45	24.480376636993483	23.036635759955594	26.272246321690208	26.21074128136072
46	23.842882890291456	25.23968120172968	27.119268381269574	23.798167526709292
47	21.03797893409538	25.400896363150427	30.546589639741505	23.014535063012683
48	21.825894478594876	23.41628804232369	29.292674903887665	25.465142575193767
49	22.805520719831694	23.397956456487325	30.27864010471276	23.517882718968224
50	21.784948226119255	22.62049162914688	29.50511571171097	26.089444433022894
51	21.490443590112594	22.38663541730913	27.475106734373604	28.64781425820467
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	163.0
1	320.0
2	477.0
3	815.0
4	1153.0
5	890.5
6	628.0
7	636.0
8	644.0
9	742.0
10	840.0
11	835.0
12	830.0
13	930.0
14	1030.0
15	983.5
16	937.0
17	988.5
18	1040.0
19	1094.5
20	1149.0
21	1283.0
22	1417.0
23	1511.0
24	1605.0
25	2708.5
26	4581.5
27	5351.0
28	6366.5
29	7382.0
30	8484.0
31	9586.0
32	10290.5
33	10995.0
34	12365.5
35	13736.0
36	14373.0
37	15010.0
38	16399.5
39	17789.0
40	20062.5
41	22336.0
42	25310.5
43	28285.0
44	32087.0
45	35889.0
46	43043.5
47	50198.0
48	58429.5
49	66661.0
50	65468.0
51	64275.0
52	54883.0
53	45491.0
54	40109.5
55	34728.0
56	31284.5
57	27841.0
58	26700.5
59	25560.0
60	25156.5
61	24753.0
62	21975.0
63	19197.0
64	17355.5
65	15514.0
66	12349.0
67	9184.0
68	7724.0
69	6264.0
70	5241.0
71	4218.0
72	3435.0
73	2652.0
74	2441.0
75	1824.5
76	1419.0
77	1084.5
78	750.0
79	583.5
80	417.0
81	290.5
82	164.0
83	120.0
84	76.0
85	42.0
86	8.0
87	6.5
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	583692.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.021419887417366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.38824272043324	21.215003927931736
2	11.693530933133948	7.2549986608650325
3	4.434714091227715	4.127133837138644
4	2.2446175165739928	2.7852488987317536
5	1.4531112432841589	2.253878701052249
6	1.080010848108176	2.010208200127768
7	0.9428477607160124	2.047393339255785
8	0.8333545348011367	2.0681472751319543
9	0.7085646730040189	1.9782614014783428
>10	8.065466405577059	46.09285795240209
>50	0.09797313646217932	2.110486779406482
>100	0.04981684904856279	3.019347451719301
>500	0.0038746438148882178	0.8025722241433128
>1k	0.0038746438148882178	2.2344613506155175
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	3014	0.5163682215963213	RNA PCR Primer, Index 23 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	2620	0.44886686814278765	RNA PCR Primer, Index 30 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	2217	0.37982360560021383	RNA PCR Primer, Index 30 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1667	0.2855958279366515	RNA PCR Primer, Index 23 (96% over 25bp)
TGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCTT	1394	0.23882458556910152	RNA PCR Primer, Index 23 (96% over 27bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1061	0.18177394927461746	No Hit
ACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1040	0.17817616140019052	RNA PCR Primer, Index 23 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	906	0.15521884829670443	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCC	904	0.1548762018324733	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	669	0.11461524228531486	No Hit
GCCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTG	590	0.10108070694818501	RNA PCR Primer, Index 23 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.45794699944491274	0.0
2	0.0	0.0	0.0	1.8520041391692879	0.0
3	0.0	0.0	0.0	2.5271889969367405	0.0
4	0.0	0.0	0.0	3.376095612069379	0.0
5	0.0	0.0	0.0	5.433173660081001	0.0
6	0.0	0.0	0.0	6.530327638549098	0.0
7	0.0	0.0	0.0	7.720167485591716	0.0
8	0.0	0.0	0.0	9.488737210720723	0.0
9	0.0	0.0	0.0	10.243587371421913	0.0
10	0.0	0.0	0.0	11.933519733009875	0.0
11	0.0	0.0	0.0	14.150099710121092	0.0
12	0.0	0.0	0.0	15.746489586973953	0.0
13	0.0	0.0	0.0	16.380899515497898	0.0
14	0.0	0.0	0.0	16.64953434345511	0.0
15	0.0	0.0	0.0	17.13078130246774	0.0
16	0.0	0.0	0.0	18.10629578613378	0.0
17	0.0	0.0	0.0	19.25553202716501	0.0
18	0.0	0.0	0.0	20.53583054076465	0.0
19	0.0	0.0	0.0	21.292907903483343	0.0
20	0.0	0.0	0.0	22.00938166019065	0.0
21	0.0	0.0	0.0	22.873536042981574	0.0
22	0.0	0.0	0.0	23.74334409243231	0.0
23	0.0	0.0	0.0	24.5740904449607	0.0
24	0.0	0.0	0.0	25.288508322882617	0.0
25	0.0	0.0	0.0	25.896534473660765	0.0
26	0.0	0.0	0.0	26.4526496851079	0.0
27	0.0	0.0	0.0	26.980839209720195	0.0
28	0.0	0.0	0.0	27.525818411079815	0.0
29	0.0	0.0	0.0	28.07490937001021	0.0
30	0.0	0.0	0.0	28.67796714705701	0.0
31	0.0	0.0	0.0	29.253784530197432	0.0
32	1.713232321155678E-4	0.0	0.0	29.828745297177278	0.0
33	1.713232321155678E-4	0.0	0.0	30.357448791485922	0.0
34	1.713232321155678E-4	0.0	0.0	30.880327295902635	0.0
35	1.713232321155678E-4	0.0	0.0	31.426848406351294	0.0
36	1.713232321155678E-4	0.0	0.0	31.956751163284746	0.0
37	1.713232321155678E-4	0.0	0.0	32.478259081844534	0.0
38	1.713232321155678E-4	0.0	0.0	32.983491293353346	0.0
39	1.713232321155678E-4	0.0	0.0	33.50054480787813	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCCG	30	2.1636588E-6	45.000004	12
AACCGTA	20	7.030431E-4	45.0	1
GGTACGT	20	7.030431E-4	45.0	39
TAACGCG	20	7.030431E-4	45.0	1
TATTGCG	20	7.030431E-4	45.0	1
GTATGCG	25	3.8884384E-5	45.0	1
GTCGTTT	20	7.030431E-4	45.0	9
CAATGCG	35	1.2105556E-7	45.0	1
CGGTATG	20	7.030431E-4	45.0	2
GTACGTG	20	7.030431E-4	45.0	40
TGCGTCG	25	3.8884384E-5	45.0	1
CTACGAA	140	0.0	40.17857	11
GTCGATG	45	1.9255822E-8	40.0	1
CAACCGG	45	1.9255822E-8	40.0	2
GTGGCGT	130	0.0	39.807693	34
AAGGGCG	210	0.0	39.642857	5
TGGCGTT	125	0.0	39.600002	35
AACGGGA	155	0.0	39.193546	4
TCGATGG	75	0.0	39.0	2
TTGTGCG	70	0.0	38.571426	1
>>END_MODULE