FastQCFastQC Report
Sat 18 Jun 2016
SRR3552498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266024
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC30221.1359877304303372No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT19730.7416624063994226Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC15850.5958109042793132No Hit
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC13480.5067211980873906No Hit
TGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT12430.46725107509096925Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCC8100.3044838059723935No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.2988452169729047No Hit
ACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC7500.28192944997443836No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG5290.19885423871530386No Hit
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG4640.17442035305085254No Hit
GGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG4440.16690223438486754No Hit
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG3330.12517667578865063No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTC3210.12066580458905965No Hit
GGCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT3110.11690674525606712No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG2990.1123958740564761No Hit
TGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG2860.10750909692358583No Hit
GACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT2860.10750909692358583No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA302.1591914E-645.0000045
GCGTCCC302.1591914E-645.0000047
AAGTACG207.023503E-445.01
TCCGCGG207.023503E-445.02
GGTAACT253.8826973E-545.08
ACGGGAC453.8198777E-1045.05
TTGACCC253.8826973E-545.08
TGTTACG253.8826973E-545.01
GCGATGT253.8826973E-545.09
AGGGCGC207.023503E-445.06
CGTTTGG453.8198777E-1045.02
GGAACAC700.041.7857178
TACGGGA501.0750227E-940.54
CGGGATA501.0750227E-940.56
AGTACGG501.0750227E-940.52
CGTTTTT5450.040.4587171
GAGCGAC451.91867E-840.018
TCGAAGG451.91867E-840.02
CAGGGGT1300.039.8076935
GACGGGA850.039.7058834