##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552498_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	266024
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.356486632785014	33.0	31.0	34.0	30.0	34.0
2	32.511465130965625	34.0	31.0	34.0	31.0	34.0
3	32.545165097885906	34.0	31.0	34.0	30.0	34.0
4	36.0206560310348	37.0	35.0	37.0	35.0	37.0
5	36.06671202598262	37.0	35.0	37.0	35.0	37.0
6	36.10858418789282	37.0	35.0	37.0	35.0	37.0
7	36.21442050341322	37.0	36.0	37.0	35.0	37.0
8	36.22185216371455	37.0	37.0	37.0	35.0	37.0
9	37.722472408504494	39.0	38.0	39.0	35.0	39.0
10	37.74259089405467	39.0	37.0	39.0	35.0	39.0
11	37.701718641927044	39.0	37.0	39.0	35.0	39.0
12	37.62236114034824	39.0	37.0	39.0	35.0	39.0
13	37.58378567347307	39.0	37.0	39.0	35.0	39.0
14	38.81134408925511	40.0	38.0	41.0	35.0	41.0
15	38.89072414518991	40.0	38.0	41.0	35.0	41.0
16	38.93884386371155	40.0	38.0	41.0	35.0	41.0
17	38.94331338525847	40.0	38.0	41.0	35.0	41.0
18	38.93809956996361	40.0	38.0	41.0	35.0	41.0
19	38.909786335067515	40.0	38.0	41.0	35.0	41.0
20	38.851656241542116	40.0	38.0	41.0	35.0	41.0
21	38.784515682795536	40.0	38.0	41.0	35.0	41.0
22	38.78799281267855	40.0	38.0	41.0	35.0	41.0
23	38.76754352990707	40.0	38.0	41.0	35.0	41.0
24	38.76121703304965	40.0	38.0	41.0	35.0	41.0
25	38.69968874988723	40.0	38.0	41.0	35.0	41.0
26	38.70731212221454	40.0	38.0	41.0	35.0	41.0
27	38.6871410098337	40.0	38.0	41.0	35.0	41.0
28	38.659628454575525	40.0	38.0	41.0	35.0	41.0
29	38.63754022193486	40.0	38.0	41.0	35.0	41.0
30	38.53299702282501	40.0	38.0	41.0	35.0	41.0
31	38.41899227137401	40.0	38.0	41.0	35.0	41.0
32	38.33391348149039	40.0	38.0	41.0	34.0	41.0
33	38.12160556942231	40.0	37.0	41.0	34.0	41.0
34	38.05437855231107	40.0	37.0	41.0	34.0	41.0
35	37.988384506661056	40.0	37.0	41.0	34.0	41.0
36	37.86340330195772	40.0	37.0	41.0	34.0	41.0
37	37.848171593540435	40.0	37.0	41.0	34.0	41.0
38	37.78466228610952	40.0	37.0	41.0	34.0	41.0
39	37.7117402941088	40.0	37.0	41.0	33.0	41.0
40	37.69689952786215	40.0	37.0	41.0	33.0	41.0
41	37.60969686945539	40.0	36.0	41.0	33.0	41.0
42	37.5360606561814	40.0	36.0	41.0	33.0	41.0
43	37.52670059844225	40.0	36.0	41.0	33.0	41.0
44	37.517690133221066	40.0	36.0	41.0	33.0	41.0
45	37.54774757164767	40.0	36.0	41.0	33.0	41.0
46	37.45796243947915	40.0	36.0	41.0	33.0	41.0
47	37.379165037740954	40.0	35.0	41.0	33.0	41.0
48	37.34975039846029	40.0	35.0	41.0	33.0	41.0
49	37.327169729047	39.0	35.0	41.0	33.0	41.0
50	37.28521110877214	39.0	35.0	41.0	33.0	41.0
51	36.72576910353953	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	3.0
14	1.0
15	2.0
16	4.0
17	7.0
18	22.0
19	52.0
20	89.0
21	156.0
22	288.0
23	439.0
24	804.0
25	1156.0
26	1363.0
27	1613.0
28	1862.0
29	2100.0
30	2475.0
31	3062.0
32	4145.0
33	5856.0
34	12043.0
35	17826.0
36	15659.0
37	24322.0
38	49899.0
39	120532.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3661925239828	20.99885724596277	25.5522058160166	12.082744414037832
2	32.36061407993264	24.090683547349112	25.08570655279223	18.46299581992602
3	31.091931555047665	22.873500135326136	29.03008751090127	17.004480798724927
4	28.52900490181337	24.831970047815236	26.79307130183743	19.84595374853397
5	24.42185667458575	27.88545394400505	26.533320301927642	21.15936907948155
6	25.051499112861997	31.459191651881035	26.31491895468078	17.174390280576187
7	73.96024418849427	6.207334676570535	12.999954891288004	6.83246624364719
8	71.63376236730521	5.3220762036507985	13.841608275945028	9.202553153098968
9	65.0102998225724	7.664721979971732	17.028538778456078	10.296439418999789
10	36.55760382521878	21.701049529365772	24.650783387964996	17.090563257450455
11	25.342074399302316	25.39094217063122	27.784335247947556	21.482648182118908
12	22.93702823805371	21.672480678435026	34.91000811956816	20.480482963943103
13	22.225814212251528	24.14218266021111	35.12615403121523	18.505849096322137
14	17.809295401918625	27.255811505728804	32.226039755811506	22.70885333654106
15	16.47746068023938	25.471386040357263	37.051168315640695	20.999984963762667
16	17.494286229813852	26.782921841638345	32.01590833909723	23.706883589450577
17	17.079286079451478	25.975851802844858	30.5709259314949	26.373936186208763
18	18.405106306197936	24.99135416353412	34.62807866959372	21.975460860674225
19	18.780636333563887	26.89757315129462	31.26484828436532	23.05694223077617
20	19.513652903497427	28.55607012901092	31.326120951493095	20.604156015998555
21	19.06557303100472	28.289552822301744	32.062896580759634	20.5819775659339
22	17.592021772471654	26.548356539259615	28.83048146031937	27.029140227949355
23	17.185291552641868	26.277328361350854	30.834811896670978	25.7025681893363
24	20.87255285237422	24.50342826211169	29.00227047183713	25.621748413676958
25	16.840961717739752	27.256563317595408	28.33691696989745	27.56555799476739
26	16.497759600637536	26.267178901151777	30.488602532102366	26.74645896610832
27	19.409151054040237	25.11014043845668	30.76714882867711	24.71355967882597
28	15.213664932487294	24.428998887318436	29.97661865094879	30.380717529245484
29	17.968303611704208	22.92725468378793	28.191441373710642	30.913000330797217
30	20.37071843141972	25.53228280155174	28.030929540191863	26.066069226836674
31	20.47522028087691	26.331458815745947	24.32412113192795	28.869199771449196
32	20.448906865545965	24.65942922443088	24.690253510961416	30.20141039906174
33	19.33284214958049	25.453342555558894	27.694869635822332	27.51894565903828
34	20.681216732324902	21.552942591645866	28.05799476738941	29.707845908639825
35	20.716551890055033	21.60632123417436	27.48511412504135	30.19201275072926
36	20.67670586112531	23.938441644362914	30.337112440982768	25.047740053529004
37	21.23981294920759	22.441584217965296	30.485595284635973	25.83300754819114
38	20.415451237482333	25.707830872402486	26.012690584308185	27.864027305806992
39	23.005443117914172	22.81297928006496	28.67673593359998	25.504841668420898
40	26.494601990797822	21.47061919225333	28.756052085526118	23.27872673142273
41	23.143400595434997	21.929600336811717	27.988828075662347	26.93817099209094
42	21.62624424863922	19.465912849968422	32.416999969927524	26.49084293146483
43	21.789763329624396	19.411782395573333	32.03169638829579	26.766757886506483
44	19.352765164045348	21.25560099840616	28.54479295101194	30.846840886536555
45	21.701049529365772	19.45613929570264	27.3595615433194	31.483249631612185
46	24.267735241933057	21.34055573933179	29.340585811806452	25.0511232069287
47	19.453883860102845	20.486873364809192	34.42546537154542	25.633777403542535
48	19.987294379454486	19.206161850058642	32.01929149249692	28.787252277989957
49	21.589781373109194	17.481881334014975	34.21984482603073	26.708492466845097
50	20.620319971130424	17.226265299371484	32.678630499503804	29.474784229994288
51	20.15457251977265	17.251826902835834	28.539906173879046	34.053694403512466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	106.0
1	161.5
2	217.0
3	798.5
4	1380.0
5	926.0
6	472.0
7	532.0
8	592.0
9	601.5
10	611.0
11	691.0
12	771.0
13	733.0
14	695.0
15	717.5
16	740.0
17	764.5
18	789.0
19	818.5
20	848.0
21	836.0
22	824.0
23	896.0
24	968.0
25	1191.0
26	1775.0
27	2136.0
28	2382.5
29	2629.0
30	3128.0
31	3627.0
32	4006.5
33	4386.0
34	4909.5
35	5433.0
36	5864.0
37	6295.0
38	6534.5
39	6774.0
40	7593.0
41	8412.0
42	9456.0
43	10500.0
44	12123.5
45	13747.0
46	15698.0
47	17649.0
48	23437.5
49	29226.0
50	35281.5
51	41337.0
52	39787.5
53	38238.0
54	30435.5
55	22633.0
56	17970.0
57	13307.0
58	10917.5
59	8528.0
60	7245.5
61	5963.0
62	5145.0
63	4327.0
64	3851.5
65	3376.0
66	2829.0
67	2282.0
68	1897.5
69	1513.0
70	1316.5
71	1120.0
72	965.0
73	810.0
74	684.5
75	456.0
76	353.0
77	302.5
78	252.0
79	184.5
80	117.0
81	83.5
82	50.0
83	33.0
84	16.0
85	9.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	266024.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.498658681293314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.87091524163245	26.271850547139803
2	14.922553421543983	12.08686795344951
3	6.22014350745708	7.557224065715008
4	3.561199652119001	5.7689523682843165
5	2.320251548950637	4.698353776784699
6	1.70588493660749	4.145163107833585
7	1.3384069430192533	3.7942580173406952
8	1.0435063734356331	3.3808486759619125
9	0.7972723543508963	2.9059614859399274
>10	3.0778861185379904	18.167574034927974
>50	0.07744358309759142	2.263005263163335
>100	0.05560052119827076	4.1126541989400485
>500	0.003971465799876483	1.1596494632130523
>1k	0.0049643322498456035	3.687637041306138
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	3022	1.1359877304303372	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	1973	0.7416624063994226	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1585	0.5958109042793132	No Hit
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1348	0.5067211980873906	No Hit
TGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT	1243	0.46725107509096925	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCC	810	0.3044838059723935	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	795	0.2988452169729047	No Hit
ACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	750	0.28192944997443836	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	529	0.19885423871530386	No Hit
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	464	0.17442035305085254	No Hit
GGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	444	0.16690223438486754	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	333	0.12517667578865063	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTC	321	0.12066580458905965	No Hit
GGCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	311	0.11690674525606712	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG	299	0.1123958740564761	No Hit
TGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	286	0.10750909692358583	No Hit
GACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	286	0.10750909692358583	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.759059332992512E-4	0.0	0.0	0.7582022674645896	0.0
2	3.759059332992512E-4	0.0	0.0	3.3391724054972483	0.0
3	3.759059332992512E-4	0.0	0.0	4.504856704658226	0.0
4	3.759059332992512E-4	0.0	0.0	5.96863440892551	0.0
5	3.759059332992512E-4	0.0	0.0	9.640107659459296	0.0
6	3.759059332992512E-4	0.0	0.0	11.4967070640243	0.0
7	3.759059332992512E-4	0.0	0.0	13.522840044507262	0.0
8	3.759059332992512E-4	0.0	0.0	16.54136468890025	0.0
9	3.759059332992512E-4	0.0	0.0	17.840119688449164	0.0
10	3.759059332992512E-4	0.0	0.0	20.713920548521937	0.0
11	3.759059332992512E-4	0.0	0.0	24.626349502300545	0.0
12	3.759059332992512E-4	0.0	0.0	27.345653023787328	0.0
13	3.759059332992512E-4	0.0	0.0	28.437283854088353	0.0
14	3.759059332992512E-4	0.0	0.0	28.89588909271344	0.0
15	3.759059332992512E-4	0.0	0.0	29.727393017171384	0.0
16	3.759059332992512E-4	0.0	0.0	31.467461582413616	0.0
17	3.759059332992512E-4	0.0	0.0	33.41390250503714	0.0
18	3.759059332992512E-4	0.0	0.0	35.735121643160014	0.0
19	3.759059332992512E-4	0.0	0.0	36.99403061377921	0.0
20	3.759059332992512E-4	0.0	0.0	38.16986437313926	0.0
21	3.759059332992512E-4	0.0	0.0	39.54455237121463	0.0
22	3.759059332992512E-4	0.0	0.0	40.90721137942441	0.0
23	7.518118665985024E-4	0.0	0.0	42.18153249330887	0.0
24	0.0011277177998977536	0.0	0.0	43.19121583015066	0.0
25	0.0015036237331970048	0.0	0.0	44.10241181246805	0.0
26	0.0015036237331970048	0.0	0.0	44.89219017832978	0.0
27	0.001879529666496256	0.0	0.0	45.66204552972664	0.0
28	0.001879529666496256	0.0	0.0	46.43904309385619	0.0
29	0.001879529666496256	0.0	0.0	47.20777072745316	0.0
30	0.001879529666496256	0.0	0.0	48.020479355246145	0.0
31	0.001879529666496256	0.0	0.0	48.78018524644393	0.0
32	0.001879529666496256	0.0	0.0	49.53913932577512	0.0
33	0.001879529666496256	0.0	0.0	50.243962950711214	0.0
34	0.001879529666496256	0.0	0.0	50.89691155685201	0.0
35	0.001879529666496256	0.0	0.0	51.599479746188315	0.0
36	0.001879529666496256	0.0	0.0	52.24791748112952	0.0
37	0.001879529666496256	0.0	0.0	52.877184013472466	0.0
38	0.001879529666496256	0.0	0.0	53.5135927585481	0.0
39	0.001879529666496256	0.0	0.0	54.190223438486754	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	30	2.1591914E-6	45.000004	5
GCGTCCC	30	2.1591914E-6	45.000004	7
AAGTACG	20	7.023503E-4	45.0	1
TCCGCGG	20	7.023503E-4	45.0	2
GGTAACT	25	3.8826973E-5	45.0	8
ACGGGAC	45	3.8198777E-10	45.0	5
TTGACCC	25	3.8826973E-5	45.0	8
TGTTACG	25	3.8826973E-5	45.0	1
GCGATGT	25	3.8826973E-5	45.0	9
AGGGCGC	20	7.023503E-4	45.0	6
CGTTTGG	45	3.8198777E-10	45.0	2
GGAACAC	70	0.0	41.785717	8
TACGGGA	50	1.0750227E-9	40.5	4
CGGGATA	50	1.0750227E-9	40.5	6
AGTACGG	50	1.0750227E-9	40.5	2
CGTTTTT	545	0.0	40.458717	1
GAGCGAC	45	1.91867E-8	40.0	18
TCGAAGG	45	1.91867E-8	40.0	2
CAGGGGT	130	0.0	39.807693	5
GACGGGA	85	0.0	39.705883	4
>>END_MODULE