FastQCFastQC Report
Sat 18 Jun 2016
SRR3552496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370285
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC43031.162077858946487No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT23920.6459889004415518No Hit
TGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT16520.4461428359236804Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC16210.43777090619387765No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC15000.4050933740227122No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC10420.28140486382111074No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC7490.20227662476200764No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG6800.18364232955696289No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.17581052432585711No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG5580.15069473513644896No Hit
GGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG5550.1498845483884035No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5430.14664380139622182No Hit
GACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT4370.11801720296528351No Hit
AAGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC4210.11369620697570791No Hit
TGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG3950.10667458849264756No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG3940.10640452624329907No Hit
GGCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT3730.10073321900698111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAATTC351.2089367E-745.00000435
TACCCTA207.0270855E-445.015
TCGCAAA207.0270855E-445.035
CCCTATT207.0270855E-445.017
GAATAAG253.8856662E-545.01
TCTAGAG207.0270855E-445.01
CACTAGG207.0270855E-445.02
TACGACA253.8856662E-545.032
ACGGATG207.0270855E-445.01
CATACGA406.7939254E-945.018
CCAAGCG207.0270855E-445.020
TAGGACG207.0270855E-445.01
GCTAATG207.0270855E-445.01
CGCCGGT453.8380676E-1045.028
CGCAAAC207.0270855E-445.036
TGAGCGT207.0270855E-445.016
TCGCGAG207.0270855E-445.01
ACGACAA253.8856662E-545.033
GTTGCGC207.0270855E-445.028
TAGCGAG302.1615015E-644.9999961