##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	370285
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37671253223868	33.0	31.0	34.0	30.0	34.0
2	32.51446048314136	34.0	31.0	34.0	31.0	34.0
3	32.5667553371052	34.0	31.0	34.0	31.0	34.0
4	36.03152436636645	37.0	35.0	37.0	35.0	37.0
5	36.074115883711194	37.0	35.0	37.0	35.0	37.0
6	36.11508702755985	37.0	35.0	37.0	35.0	37.0
7	36.19660801814818	37.0	36.0	37.0	35.0	37.0
8	36.19046410197551	37.0	37.0	37.0	35.0	37.0
9	37.64511929999865	39.0	38.0	39.0	35.0	39.0
10	37.730688523704714	39.0	37.0	39.0	35.0	39.0
11	37.70252103109767	39.0	37.0	39.0	35.0	39.0
12	37.59966242218832	39.0	37.0	39.0	35.0	39.0
13	37.54819935995247	39.0	37.0	39.0	35.0	39.0
14	38.79121757565119	40.0	38.0	41.0	35.0	41.0
15	38.85868452678342	40.0	38.0	41.0	35.0	41.0
16	38.911624829523205	40.0	38.0	41.0	35.0	41.0
17	38.91679652159823	40.0	38.0	41.0	35.0	41.0
18	38.91589991493039	40.0	38.0	41.0	35.0	41.0
19	38.8877108173434	40.0	38.0	41.0	35.0	41.0
20	38.8412871166804	40.0	38.0	41.0	35.0	41.0
21	38.772183588317105	40.0	38.0	41.0	35.0	41.0
22	38.77561337888383	40.0	38.0	41.0	35.0	41.0
23	38.756560487192296	40.0	38.0	41.0	35.0	41.0
24	38.72888990912406	40.0	38.0	41.0	35.0	41.0
25	38.671334242542905	40.0	38.0	41.0	35.0	41.0
26	38.655889382502664	40.0	38.0	41.0	35.0	41.0
27	38.636488110509475	40.0	38.0	41.0	35.0	41.0
28	38.58790661247418	40.0	38.0	41.0	35.0	41.0
29	38.56129737904587	40.0	38.0	41.0	35.0	41.0
30	38.479095831589184	40.0	38.0	41.0	35.0	41.0
31	38.39994598755013	40.0	38.0	41.0	35.0	41.0
32	38.28534237141661	40.0	37.0	41.0	34.0	41.0
33	38.189805150087096	40.0	37.0	41.0	34.0	41.0
34	38.12269738174649	40.0	37.0	41.0	34.0	41.0
35	38.08343573193621	40.0	37.0	41.0	34.0	41.0
36	38.034017040927935	40.0	37.0	41.0	34.0	41.0
37	37.974365691291844	40.0	37.0	41.0	34.0	41.0
38	37.92690225096885	40.0	37.0	41.0	34.0	41.0
39	37.878264039861186	40.0	37.0	41.0	34.0	41.0
40	37.81246877405242	40.0	37.0	41.0	34.0	41.0
41	37.7541569331731	40.0	36.0	41.0	34.0	41.0
42	37.719786110698514	40.0	36.0	41.0	34.0	41.0
43	37.6635456472717	40.0	36.0	41.0	33.0	41.0
44	37.589286630568346	40.0	36.0	41.0	33.0	41.0
45	37.558248376250724	40.0	36.0	41.0	33.0	41.0
46	37.458638616201036	40.0	35.0	41.0	33.0	41.0
47	37.38591085245149	40.0	35.0	41.0	33.0	41.0
48	37.3648919075847	40.0	35.0	41.0	33.0	41.0
49	37.335417313690805	39.0	35.0	41.0	33.0	41.0
50	37.25849818383137	39.0	35.0	41.0	33.0	41.0
51	36.63121649540219	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	1.0
15	3.0
16	15.0
17	19.0
18	40.0
19	67.0
20	100.0
21	218.0
22	387.0
23	661.0
24	1130.0
25	1595.0
26	1926.0
27	2100.0
28	2339.0
29	2701.0
30	3417.0
31	4297.0
32	5794.0
33	8177.0
34	16363.0
35	26860.0
36	22002.0
37	33608.0
38	65966.0
39	170081.0
40	416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31736365232186	21.513158783099502	25.938128738674266	11.231348825904371
2	31.355037336105973	25.36397639655941	25.322116747910396	17.95886951942423
3	31.382583685539515	24.293179577892705	27.210662057604278	17.1135746789635
4	29.682001701392167	25.474971981041627	25.73693236290965	19.106093954656547
5	24.894878269441108	28.286049934509904	25.314555004928636	21.504516791120352
6	24.801166668917183	33.64705564632648	24.591868425672118	16.95990925908422
7	74.39809876176459	7.470732003726859	11.601334107511782	6.529835126996773
8	72.37614270089256	6.410197550535399	12.174406200629246	9.039253547942801
9	65.97998838732327	8.90125173852573	15.108362477551077	10.010397396599917
10	37.479509026830684	23.966674318430396	22.410845699933834	16.142970954805083
11	27.50151910015258	25.577595635794047	25.199778548955532	21.72110671509783
12	24.615633903614782	21.670064949970968	33.24088202330637	20.473419123107877
13	23.08465101205828	24.7277097370944	33.24979407753487	18.937845173312446
14	17.943475971211363	28.2247458039078	30.27316796521598	23.558610259664853
15	16.510255613919007	26.146616795171283	36.30959936265309	21.03352822825661
16	18.038267820732678	26.818531671550293	31.59080167978719	23.55239882792984
17	17.57970212133897	26.229525905721268	29.400326775321712	26.79044519761805
18	18.15142390320969	25.831184087932268	33.34917698529511	22.668215023562933
19	20.017283983958304	27.394304387161238	29.183466789094886	23.40494483978557
20	20.935225569493767	28.50290992073673	29.57775767314366	20.984106836625845
21	20.40374306277597	28.6498237843823	29.957465195727618	20.988967957114117
22	18.65238937575111	27.313555774606048	26.67890948863713	27.35514536100571
23	18.316972062060305	27.290870545660773	28.704916483249388	25.68724090902953
24	20.84448465371268	24.844106566563593	28.831845740443175	25.479563039280556
25	17.461954980623034	27.976288534507205	26.70024440633566	27.8615120785341
26	17.16542663083841	27.01756754932012	29.080303009843767	26.736702809997702
27	20.26547119110955	26.590599133100177	27.209041684108186	25.934887991682082
28	15.892623249659046	25.957033096128658	30.20403202938277	27.946311624829523
29	18.695599335646868	25.487934969010357	26.79638656710372	29.020079128239058
30	20.247377020403203	24.948080532562756	25.118219749652294	29.686322697381744
31	20.58576501883684	27.491256734677343	24.56999338347489	27.352984863010928
32	18.995098370174325	28.402176701729747	23.014704889477024	29.5880200386189
33	19.8584873813414	26.57223490014448	23.172151180847187	30.397126537666928
34	19.75397329084354	23.9042899388309	27.323007953333246	29.01872881699232
35	18.079587344882995	24.61995489960436	23.506488245540595	33.79396950997205
36	19.168748396505393	26.644881645219222	24.65155218277813	29.534817775497253
37	19.88846429101908	26.189826755067042	28.748666567643845	25.173042386270033
38	17.92106080451544	28.716529159971373	25.12227068339252	28.240139352120664
39	22.11242691440377	25.001282795684403	26.27138555437028	26.614904735541543
40	23.366596000378088	21.75486449626639	27.078871679922223	27.7996678234333
41	19.344018796332556	24.353403459497414	24.111157621831833	32.1914201223382
42	19.41018404742293	23.67554721363274	27.797777387687862	29.116491351256464
43	21.20420756984485	23.38604048233118	28.347354065112008	27.062397882711963
44	19.22411115762183	23.366866062627437	27.898510606694842	29.510512173055893
45	20.7532036134329	21.5404350702837	26.603562121068908	31.102799195214498
46	22.18372334823177	21.87720269522125	27.820462616633137	28.11861133991385
47	18.42256640155556	22.87886357805474	31.418231902453513	27.280338117936186
48	18.22623114627922	21.467248200710266	29.57046599241125	30.736054660599265
49	20.595487259813385	20.544715556935873	31.901643328787287	26.95815385446345
50	19.681596608018147	19.374265768259583	30.96587763479482	29.978259988927448
51	18.742050042534807	18.920561189354146	27.301402973385365	35.03598579472568
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	145.0
1	256.5
2	368.0
3	831.0
4	1294.0
5	922.5
6	551.0
7	602.0
8	653.0
9	725.5
10	798.0
11	822.0
12	846.0
13	851.0
14	856.0
15	868.0
16	880.0
17	937.5
18	995.0
19	1161.0
20	1327.0
21	1235.0
22	1143.0
23	1487.0
24	1831.0
25	2209.0
26	2829.5
27	3072.0
28	3645.5
29	4219.0
30	4955.5
31	5692.0
32	5808.0
33	5924.0
34	6834.0
35	7744.0
36	7730.0
37	7716.0
38	8753.0
39	9790.0
40	11115.0
41	12440.0
42	13710.0
43	14980.0
44	16595.5
45	18211.0
46	21195.0
47	24179.0
48	31287.5
49	38396.0
50	45553.5
51	52711.0
52	51081.0
53	49451.0
54	40121.0
55	30791.0
56	25332.5
57	19874.0
58	16811.0
59	13748.0
60	12080.0
61	10412.0
62	9547.5
63	8683.0
64	7512.5
65	6342.0
66	4958.5
67	3575.0
68	3127.5
69	2680.0
70	2288.5
71	1897.0
72	1623.0
73	1349.0
74	1112.0
75	743.5
76	612.0
77	442.5
78	273.0
79	242.0
80	211.0
81	160.5
82	110.0
83	68.0
84	26.0
85	24.0
86	22.0
87	14.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	370285.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.37976139828474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.84833143113408	25.361544401675353
2	13.280351086015823	9.928327097614464
3	5.3994102951957315	6.054860055775759
4	2.793188921601624	4.176349417192039
5	1.7916844371250356	3.348636838037696
6	1.351540590210388	3.0312158869296746
7	1.0774857584586413	2.819331239286371
8	0.8240676445958346	2.4642761544831044
9	0.7326496804712556	2.4647643220090583
>10	4.7405937449808695	29.201869443354333
>50	0.0883106398455244	2.2668880946858097
>100	0.06369947792136187	4.486154763653703
>500	0.004343146221911036	1.0108729913757681
>1k	0.004343146221911036	3.3849092939268894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	4303	1.162077858946487	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	2392	0.6459889004415518	No Hit
TGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT	1652	0.4461428359236804	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1621	0.43777090619387765	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1500	0.4050933740227122	No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1042	0.28140486382111074	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC	749	0.20227662476200764	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	680	0.18364232955696289	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	651	0.17581052432585711	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	558	0.15069473513644896	No Hit
GGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	555	0.1498845483884035	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	543	0.14664380139622182	No Hit
GACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT	437	0.11801720296528351	No Hit
AAGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	421	0.11369620697570791	No Hit
TGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	395	0.10667458849264756	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	394	0.10640452624329907	No Hit
GGCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT	373	0.10073321900698111	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.700622493484748E-4	0.0	0.0	0.6624626976518088	0.0
2	2.700622493484748E-4	0.0	0.0	3.0076832709939643	0.0
3	2.700622493484748E-4	0.0	0.0	4.070648284429561	0.0
4	2.700622493484748E-4	0.0	0.0	5.452556814345707	0.0
5	2.700622493484748E-4	0.0	0.0	8.844808728411898	0.0
6	2.700622493484748E-4	0.0	0.0	10.564295070013639	0.0
7	2.700622493484748E-4	0.0	0.0	12.388565564362585	0.0
8	2.700622493484748E-4	0.0	0.0	15.322791903533764	0.0
9	2.700622493484748E-4	0.0	0.0	16.526999473378613	0.0
10	2.700622493484748E-4	0.0	0.0	19.045059886303793	0.0
11	2.700622493484748E-4	0.0	0.0	22.500506366717527	0.0
12	2.700622493484748E-4	0.0	0.0	25.114168815912066	0.0
13	2.700622493484748E-4	0.0	0.0	26.1617402811348	0.0
14	5.401244986969496E-4	0.0	0.0	26.601671685323467	0.0
15	5.401244986969496E-4	0.0	0.0	27.330569696315	0.0
16	5.401244986969496E-4	0.0	0.0	28.863172961367596	0.0
17	5.401244986969496E-4	0.0	0.0	30.624248889369	0.0
18	5.401244986969496E-4	0.0	0.0	32.78879781789703	0.0
19	5.401244986969496E-4	0.0	0.0	33.92792038564889	0.0
20	5.401244986969496E-4	0.0	0.0	35.04327747545809	0.0
21	5.401244986969496E-4	0.0	0.0	36.3074388646583	0.0
22	0.0010802489973938993	0.0	0.0	37.55539651889761	0.0
23	0.0010802489973938993	0.0	0.0	38.72881699231673	0.0
24	0.0010802489973938993	0.0	0.0	39.659181441322225	0.0
25	0.001620373496090849	0.0	0.0	40.478280243596146	0.0
26	0.001620373496090849	0.0	0.0	41.19043439512808	0.0
27	0.0018904357454393238	0.0	0.0	41.870451138987534	0.0
28	0.0018904357454393238	0.0	0.0	42.56343087081572	0.0
29	0.0018904357454393238	0.0	0.0	43.27261433760482	0.0
30	0.0018904357454393238	0.0	0.0	44.06875784868412	0.0
31	0.0018904357454393238	0.0	0.0	44.82736270710399	0.0
32	0.0018904357454393238	0.0	0.0	45.57597526229796	0.0
33	0.0018904357454393238	0.0	0.0	46.22547497198104	0.0
34	0.0024305602441362733	0.0	0.0	46.88253642464588	0.0
35	0.002700622493484748	0.0	0.0	47.51583239936805	0.0
36	0.002700622493484748	0.0	0.0	48.19773957897295	0.0
37	0.002700622493484748	0.0	0.0	48.85048003564822	0.0
38	0.002700622493484748	0.0	0.0	49.539138771486826	0.0
39	0.002700622493484748	0.0	0.0	50.2175351418502	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAATTC	35	1.2089367E-7	45.000004	35
TACCCTA	20	7.0270855E-4	45.0	15
TCGCAAA	20	7.0270855E-4	45.0	35
CCCTATT	20	7.0270855E-4	45.0	17
GAATAAG	25	3.8856662E-5	45.0	1
TCTAGAG	20	7.0270855E-4	45.0	1
CACTAGG	20	7.0270855E-4	45.0	2
TACGACA	25	3.8856662E-5	45.0	32
ACGGATG	20	7.0270855E-4	45.0	1
CATACGA	40	6.7939254E-9	45.0	18
CCAAGCG	20	7.0270855E-4	45.0	20
TAGGACG	20	7.0270855E-4	45.0	1
GCTAATG	20	7.0270855E-4	45.0	1
CGCCGGT	45	3.8380676E-10	45.0	28
CGCAAAC	20	7.0270855E-4	45.0	36
TGAGCGT	20	7.0270855E-4	45.0	16
TCGCGAG	20	7.0270855E-4	45.0	1
ACGACAA	25	3.8856662E-5	45.0	33
GTTGCGC	20	7.0270855E-4	45.0	28
TAGCGAG	30	2.1615015E-6	44.999996	1
>>END_MODULE