SRR3552495_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552495_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259784
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	2027	0.7802636036091523	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1597	0.614741477535183	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1096	0.42188895390016323	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1056	0.4064915468235149	No Hit
TGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT	1013	0.38993933421611804	Illumina Single End Adapter 1 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	682	0.2625257906568534	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	624	0.24019955039571333	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	610	0.23481045791888647	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC	575	0.22133772672681917	No Hit
GGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	475	0.1828442090351985	No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	411	0.1582083577125612	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	405	0.15589874665106396	No Hit
AAGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	382	0.1470452375819912	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	363	0.13973146922058324	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	363	0.13973146922058324	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	353	0.13588211745142117	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	339	0.13049302497459428	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	318	0.12240938625935392	No Hit
GGCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	310	0.11932990484402425	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC	267	0.10277769223662735	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	267	0.10277769223662735	No Hit
GACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	264	0.10162288670587873	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTCGT	50	2.1827873E-11	45.0	14
CCGATTT	20	7.0231955E-4	45.0	11
CACGGGC	40	6.7793735E-9	45.0	4
ACGCATT	25	3.8824433E-5	45.0	17
GGCACAT	25	3.8824433E-5	45.0	8
CGCATGG	25	3.8824433E-5	45.0	2
CGCATAG	20	7.0231955E-4	45.0	2
ATTACGG	35	1.2070814E-7	45.0	2
AAGACGC	25	3.8824433E-5	45.0	38
ATGTAGG	35	1.2070814E-7	45.0	2
ACGATAG	20	7.0231955E-4	45.0	1
TACGCAT	25	3.8824433E-5	45.0	16
GTTGTAG	25	3.8824433E-5	45.0	1
GTATGAG	20	7.0231955E-4	45.0	1
CTACGAA	25	3.8824433E-5	45.0	11
GTCACAG	20	7.0231955E-4	45.0	1
GCCCTAC	20	7.0231955E-4	45.0	26
CGAATAT	20	7.0231955E-4	45.0	14
GGGCATA	25	3.8824433E-5	45.0	7
CAAGACG	25	3.8824433E-5	45.0	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content