##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552495_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259784
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.417835586487236	33.0	31.0	34.0	30.0	34.0
2	32.56986573461029	34.0	31.0	34.0	31.0	34.0
3	32.60286622732732	34.0	31.0	34.0	31.0	34.0
4	36.05569627074801	37.0	35.0	37.0	35.0	37.0
5	36.1043790225726	37.0	35.0	37.0	35.0	37.0
6	36.139550549687435	37.0	36.0	37.0	35.0	37.0
7	36.20998598835956	37.0	37.0	37.0	35.0	37.0
8	36.19987759061374	37.0	37.0	37.0	35.0	37.0
9	37.65172219998152	39.0	38.0	39.0	35.0	39.0
10	37.77453576817664	39.0	37.0	39.0	35.0	39.0
11	37.75109321590244	39.0	37.0	39.0	35.0	39.0
12	37.656129707757216	39.0	37.0	39.0	35.0	39.0
13	37.605048809780435	39.0	37.0	39.0	35.0	39.0
14	38.88196732670218	40.0	38.0	41.0	35.0	41.0
15	38.973924491115696	40.0	38.0	41.0	35.0	41.0
16	39.01804576109383	40.0	38.0	41.0	35.0	41.0
17	39.02752286514951	40.0	38.0	41.0	35.0	41.0
18	39.00245588642873	40.0	38.0	41.0	35.0	41.0
19	38.957799556554676	40.0	38.0	41.0	35.0	41.0
20	38.89333446247652	40.0	38.0	41.0	35.0	41.0
21	38.81945000461922	40.0	38.0	41.0	35.0	41.0
22	38.83361561912974	40.0	38.0	41.0	35.0	41.0
23	38.799410279308965	40.0	38.0	41.0	35.0	41.0
24	38.77687232470052	40.0	38.0	41.0	35.0	41.0
25	38.699985372463274	40.0	38.0	41.0	35.0	41.0
26	38.699115418963444	40.0	38.0	41.0	35.0	41.0
27	38.67145012779848	40.0	38.0	41.0	35.0	41.0
28	38.624830628522155	40.0	38.0	41.0	35.0	41.0
29	38.598285498722014	40.0	38.0	41.0	35.0	41.0
30	38.49719767191205	40.0	38.0	41.0	35.0	41.0
31	38.385466387460355	40.0	38.0	41.0	35.0	41.0
32	38.26825747544114	40.0	37.0	41.0	34.0	41.0
33	38.15017860992209	40.0	37.0	41.0	34.0	41.0
34	38.0659201490469	40.0	37.0	41.0	34.0	41.0
35	37.97993332922736	40.0	37.0	41.0	34.0	41.0
36	37.95684876666769	40.0	37.0	41.0	34.0	41.0
37	37.91663843808703	40.0	37.0	41.0	34.0	41.0
38	37.82909263080097	40.0	37.0	41.0	34.0	41.0
39	37.80315569858036	40.0	37.0	41.0	34.0	41.0
40	37.68165475933853	40.0	36.0	41.0	33.0	41.0
41	37.600302559049055	40.0	36.0	41.0	33.0	41.0
42	37.60032565515967	40.0	36.0	41.0	33.0	41.0
43	37.58818479967973	40.0	36.0	41.0	33.0	41.0
44	37.52411233948203	40.0	36.0	41.0	33.0	41.0
45	37.471314630616206	40.0	36.0	41.0	33.0	41.0
46	37.35019092784775	40.0	35.0	41.0	33.0	41.0
47	37.277372740430515	40.0	35.0	41.0	33.0	41.0
48	37.25950020016629	39.0	35.0	41.0	33.0	41.0
49	37.2734541003295	39.0	35.0	41.0	33.0	41.0
50	37.1843916484464	39.0	35.0	41.0	33.0	41.0
51	36.61469528531396	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	1.0
14	3.0
15	2.0
16	12.0
17	17.0
18	35.0
19	75.0
20	128.0
21	187.0
22	322.0
23	519.0
24	887.0
25	1232.0
26	1418.0
27	1597.0
28	1810.0
29	1942.0
30	2363.0
31	2860.0
32	3887.0
33	5519.0
34	10831.0
35	18161.0
36	14963.0
37	23958.0
38	46163.0
39	120571.0
40	317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.576016998737416	21.93976534351615	25.68826409632618	11.795953561420257
2	32.50585101468913	26.175976965479013	23.55572321621039	17.76244880362147
3	31.020386166969484	24.63970067440643	27.15217257413852	17.18774058448557
4	28.948280109629536	25.491947156098917	26.360745850398793	19.199026883872754
5	24.69012718258245	29.009484802759218	25.31256736365596	20.98782065100237
6	25.851091676161737	33.07440027099437	25.210174606596247	15.864333446247652
7	73.28395898130755	7.9219659409355465	12.086194684815077	6.707880392941829
8	71.94784898223139	7.1617189665260375	12.478828565269609	8.411603485972963
9	65.55869491577619	9.104486804422136	15.017860992208911	10.31895728759277
10	37.244018107350726	23.20581714039356	22.466356665537525	17.083808086718197
11	26.56437655898747	25.304868660117634	26.264512056169742	21.866242724725158
12	23.12536568841807	22.26696024389493	33.651803036368676	20.955871031318328
13	22.563745265297324	24.585424814461245	33.90047116065655	18.950358759584887
14	17.443337541957934	28.532935053736953	31.65899362547347	22.364733778831646
15	16.266205770948172	25.311027622948295	36.817509931327564	21.60525667477597
16	16.949850645151358	27.32924275551997	31.764080928771595	23.956825670557077
17	17.6943152773073	26.373833646413942	29.01679857112062	26.915052505158133
18	18.269793366797032	26.283373879838635	32.451190219567025	22.99564253379731
19	20.503957133618698	26.815354294336835	29.73662735195393	22.944061220090536
20	19.726003141071043	29.03604532996643	30.28977920118252	20.94817232778
21	19.491577618329075	28.811628121824285	30.821759615680723	20.875034644165922
22	17.720490869337606	27.347719644011946	27.91626890031719	27.015520586333263
23	18.369106642441412	27.12638191728513	29.115341976411173	25.389169463862288
24	20.312644350691343	25.69134357774151	28.63725248668124	25.358759584885902
25	17.947602623718165	28.839728389739168	27.23878298894466	25.973885997598007
26	17.587303298124596	27.412003818556958	28.592215070982046	26.4084778123364
27	21.94592430634681	26.267206602408155	27.815415883965137	23.971453207279893
28	17.36673544175161	25.707895790348907	29.140747698087644	27.784621069811845
29	19.50505034952114	24.672805099621222	27.36773627321159	28.454408277646042
30	22.41285067594617	27.90664552089428	24.867197363963907	24.81330643919564
31	20.271841221938224	29.455624672805097	22.781618575431896	27.49091552982478
32	19.546238413451174	29.028731561605024	25.29909463246389	26.125935392479903
33	20.540525975425737	28.85782034305423	25.05966495242201	25.54198872909802
34	17.829812459581806	27.650663628245002	26.669463862285593	27.8500600498876
35	19.778354325131648	26.86039171003603	26.226403473655036	27.134850491177286
36	20.953176485079915	28.062929202722266	27.25572013672897	23.72817417546885
37	20.05897206910356	27.51901579773966	29.542620022788164	22.879392110368613
38	17.206602408154467	30.023404058756505	28.34239214116343	24.4276013919256
39	20.12210143811782	27.386598096880487	30.049579650786807	22.441720814214886
40	20.23873679672343	29.025652080189694	28.559110645767255	22.176500477319617
41	18.756736365596034	29.171927447417858	26.08551719890371	25.985818988082404
42	19.911541896344655	24.411819049672033	30.994210574939178	24.68242847904413
43	22.945600960798203	21.24996150648231	31.359898993009576	24.44453853970991
44	19.50197086810581	23.533012040772334	28.618775598189266	28.346241492932588
45	20.85424814461245	23.02181812582761	28.341622270809598	27.782311458750346
46	22.20806516182675	25.791811658916636	28.32545499337911	23.674668185877497
47	17.733578665352752	25.72598774366397	32.852677609090634	23.68775598189265
48	18.232839589813075	23.957595540910912	30.889508206817972	26.92005666245804
49	20.401949311735905	20.79804760878268	33.61253964832322	25.187463431158193
50	19.08470113632864	21.00129338219444	32.56975025405722	27.3442552274197
51	18.9507436947618	20.538601299541156	28.475579712376437	32.0350752933206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	198.0
1	235.5
2	273.0
3	722.5
4	1172.0
5	836.5
6	501.0
7	544.0
8	587.0
9	622.0
10	657.0
11	715.0
12	773.0
13	733.5
14	694.0
15	697.5
16	701.0
17	743.5
18	786.0
19	787.5
20	789.0
21	846.0
22	903.0
23	1022.0
24	1141.0
25	1661.5
26	2264.0
27	2346.0
28	2866.0
29	3386.0
30	3700.5
31	4015.0
32	4387.0
33	4759.0
34	5083.0
35	5407.0
36	5976.0
37	6545.0
38	6985.0
39	7425.0
40	9087.0
41	10749.0
42	13235.5
43	15722.0
44	21450.0
45	27178.0
46	31780.0
47	36382.0
48	35229.0
49	34076.0
50	28265.5
51	22455.0
52	18681.0
53	14907.0
54	13043.5
55	11180.0
56	10168.5
57	9157.0
58	8333.5
59	7510.0
60	6924.0
61	6338.0
62	5739.0
63	5140.0
64	4731.0
65	4322.0
66	3651.0
67	2980.0
68	2601.0
69	2222.0
70	1885.5
71	1549.0
72	1356.0
73	1163.0
74	929.5
75	543.0
76	390.0
77	303.5
78	217.0
79	173.0
80	129.0
81	93.0
82	57.0
83	40.0
84	23.0
85	12.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	259784.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.58858395327849
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.87899999991429	26.579430741145615
2	12.878999999983973	9.939647454673104
3	5.154999999993585	5.967724508367091
4	2.6649999999966836	4.113543049414368
5	1.8070000001214015	3.486478560412948
6	1.2659999999992264	2.931188837089243
7	1.0319999999987217	2.7876393047813854
8	0.8509999999989409	2.62711079553593
9	0.7299999999990915	2.5352699657272417
>10	4.574999999994307	28.494968048583974
>50	0.09099999999988675	2.3955792918165475
>100	0.06099999999992408	4.560398851592776
>500	0.003999999999995021	0.961241626274971
>1k	0.004999999999993778	2.619778964584816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	2027	0.7802636036091523	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1597	0.614741477535183	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1096	0.42188895390016323	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1056	0.4064915468235149	No Hit
TGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT	1013	0.38993933421611804	Illumina Single End Adapter 1 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	682	0.2625257906568534	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	624	0.24019955039571333	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	610	0.23481045791888647	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC	575	0.22133772672681917	No Hit
GGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	475	0.1828442090351985	No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	411	0.1582083577125612	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	405	0.15589874665106396	No Hit
AAGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	382	0.1470452375819912	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	363	0.13973146922058324	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	363	0.13973146922058324	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	353	0.13588211745142117	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	339	0.13049302497459428	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	318	0.12240938625935392	No Hit
GGCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	310	0.11932990484402425	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC	267	0.10277769223662735	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	267	0.10277769223662735	No Hit
GACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	264	0.10162288670587873	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6297539494349151	0.0
2	0.0	0.0	0.0	2.5259446309241524	0.0
3	0.0	0.0	0.0	3.5748929880208173	0.0
4	0.0	0.0	0.0	4.950651310319342	0.0
5	0.0	0.0	0.0	8.32961229328981	0.0
6	0.0	0.0	0.0	10.028331229021033	0.0
7	0.0	0.0	0.0	11.81173590367382	0.0
8	3.849351769162073E-4	0.0	0.0	14.517830197394758	0.0
9	3.849351769162073E-4	0.0	0.0	15.719597819727158	0.0
10	3.849351769162073E-4	0.0	0.0	18.34601053182644	0.0
11	3.849351769162073E-4	0.0	0.0	21.86162350260216	0.0
12	3.849351769162073E-4	0.0	0.0	24.578495981276753	0.0
13	3.849351769162073E-4	0.0	0.0	25.608582514704523	0.0
14	7.698703538324146E-4	0.0	0.0	26.01314938564346	0.0
15	7.698703538324146E-4	0.0	0.0	26.74683583284575	0.0
16	7.698703538324146E-4	0.0	0.0	28.246928217288207	0.0
17	7.698703538324146E-4	0.0	0.0	29.968358328457487	0.0
18	7.698703538324146E-4	0.0	0.0	32.08742647738121	0.0
19	7.698703538324146E-4	0.0	0.0	33.18526160194623	0.0
20	7.698703538324146E-4	0.0	0.0	34.26885412496536	0.0
21	7.698703538324146E-4	0.0	0.0	35.39517445262218	0.0
22	7.698703538324146E-4	0.0	0.0	36.593092723185414	0.0
23	7.698703538324146E-4	0.0	0.0	37.62433406214394	0.0
24	0.0015397407076648292	0.0	0.0	38.4761956086595	0.0
25	0.0015397407076648292	0.0	0.0	39.22258491670003	0.0
26	0.0015397407076648292	0.0	0.0	39.93663966987959	0.0
27	0.0015397407076648292	0.0	0.0	40.621439349613524	0.0
28	0.0015397407076648292	0.0	0.0	41.285837464970896	0.0
29	0.0015397407076648292	0.0	0.0	41.93637791395929	0.0
30	0.0015397407076648292	0.0	0.0	42.66467526868475	0.0
31	0.0015397407076648292	0.0	0.0	43.315985588026976	0.0
32	0.0015397407076648292	0.0	0.0	43.987312536568844	0.0
33	0.0015397407076648292	0.0	0.0	44.63823792073415	0.0
34	0.0015397407076648292	0.0	0.0	45.24605056508484	0.0
35	0.0015397407076648292	0.0	0.0	45.86849074615834	0.0
36	0.0015397407076648292	0.0	0.0	46.46937455732455	0.0
37	0.0015397407076648292	0.0	0.0	47.024451082437714	0.0
38	0.0019246758845810365	0.0	0.0	47.641502171034396	0.0
39	0.002309611061497244	0.0	0.0	48.21505558463954	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTCGT	50	2.1827873E-11	45.0	14
CCGATTT	20	7.0231955E-4	45.0	11
CACGGGC	40	6.7793735E-9	45.0	4
ACGCATT	25	3.8824433E-5	45.0	17
GGCACAT	25	3.8824433E-5	45.0	8
CGCATGG	25	3.8824433E-5	45.0	2
CGCATAG	20	7.0231955E-4	45.0	2
ATTACGG	35	1.2070814E-7	45.0	2
AAGACGC	25	3.8824433E-5	45.0	38
ATGTAGG	35	1.2070814E-7	45.0	2
ACGATAG	20	7.0231955E-4	45.0	1
TACGCAT	25	3.8824433E-5	45.0	16
GTTGTAG	25	3.8824433E-5	45.0	1
GTATGAG	20	7.0231955E-4	45.0	1
CTACGAA	25	3.8824433E-5	45.0	11
GTCACAG	20	7.0231955E-4	45.0	1
GCCCTAC	20	7.0231955E-4	45.0	26
CGAATAT	20	7.0231955E-4	45.0	14
GGGCATA	25	3.8824433E-5	45.0	7
CAAGACG	25	3.8824433E-5	45.0	37
>>END_MODULE