##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552494_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	879697
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60596432635328	34.0	31.0	34.0	31.0	34.0
2	32.75486673252268	34.0	31.0	34.0	31.0	34.0
3	32.80545005837237	34.0	31.0	34.0	31.0	34.0
4	36.19894236310912	37.0	37.0	37.0	35.0	37.0
5	36.22972455288582	37.0	37.0	37.0	35.0	37.0
6	36.26216299475842	37.0	37.0	37.0	35.0	37.0
7	36.3445015727006	37.0	37.0	37.0	35.0	37.0
8	36.348562061709885	37.0	37.0	37.0	35.0	37.0
9	37.85760551644486	39.0	39.0	39.0	35.0	39.0
10	37.85248216147151	39.0	38.0	39.0	35.0	39.0
11	37.82082012329245	39.0	38.0	39.0	35.0	39.0
12	37.77035047294694	39.0	37.0	39.0	35.0	39.0
13	37.77221134095035	39.0	38.0	39.0	35.0	39.0
14	39.121669165633165	40.0	39.0	41.0	35.0	41.0
15	39.182266166646016	40.0	39.0	41.0	35.0	41.0
16	39.221541053339955	41.0	39.0	41.0	35.0	41.0
17	39.19993702377069	40.0	39.0	41.0	35.0	41.0
18	39.19428280419281	40.0	39.0	41.0	35.0	41.0
19	39.1871155636543	40.0	39.0	41.0	35.0	41.0
20	39.16398259855382	40.0	39.0	41.0	35.0	41.0
21	39.100884736449025	40.0	39.0	41.0	35.0	41.0
22	39.10057099205749	40.0	39.0	41.0	35.0	41.0
23	39.07209414150554	40.0	39.0	41.0	35.0	41.0
24	39.05317853761011	40.0	39.0	41.0	35.0	41.0
25	39.00215074053907	40.0	38.0	41.0	35.0	41.0
26	39.0167660001114	40.0	38.0	41.0	35.0	41.0
27	38.99718312100644	40.0	38.0	41.0	35.0	41.0
28	38.95223014287874	40.0	38.0	41.0	35.0	41.0
29	38.94275074258523	40.0	38.0	41.0	35.0	41.0
30	38.87895945990495	40.0	38.0	41.0	35.0	41.0
31	38.845615024264035	40.0	38.0	41.0	35.0	41.0
32	38.79755529460712	40.0	38.0	41.0	35.0	41.0
33	38.73256018833757	40.0	38.0	41.0	35.0	41.0
34	38.6802774137004	40.0	38.0	41.0	35.0	41.0
35	38.64700914064729	40.0	38.0	41.0	35.0	41.0
36	38.62694200389452	40.0	38.0	41.0	35.0	41.0
37	38.603392986448746	40.0	38.0	41.0	35.0	41.0
38	38.546138045258765	40.0	38.0	41.0	35.0	41.0
39	38.50053939026733	40.0	38.0	41.0	35.0	41.0
40	38.43069829725462	40.0	38.0	41.0	35.0	41.0
41	38.38966712402111	40.0	38.0	41.0	35.0	41.0
42	38.361746146684595	40.0	38.0	41.0	35.0	41.0
43	38.34076392212319	40.0	37.0	41.0	35.0	41.0
44	38.283526032258834	40.0	37.0	41.0	35.0	41.0
45	38.23293020210368	40.0	37.0	41.0	35.0	41.0
46	38.1523160815599	40.0	37.0	41.0	34.0	41.0
47	38.102995690561634	40.0	37.0	41.0	34.0	41.0
48	38.05941818603451	40.0	37.0	41.0	34.0	41.0
49	38.034016257870604	40.0	36.0	41.0	34.0	41.0
50	37.96482197847668	40.0	36.0	41.0	34.0	41.0
51	37.42511455648933	39.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	5.0
14	5.0
15	10.0
16	19.0
17	29.0
18	66.0
19	114.0
20	186.0
21	332.0
22	525.0
23	884.0
24	1425.0
25	2026.0
26	2648.0
27	3306.0
28	3959.0
29	4970.0
30	6119.0
31	8007.0
32	10896.0
33	15856.0
34	32815.0
35	53372.0
36	44047.0
37	70558.0
38	144364.0
39	471726.0
40	1422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.814056430793784	21.33541435289651	25.56903115504543	11.281498061264276
2	30.831524945521018	24.357250280494306	26.246537159953938	18.56468761403074
3	32.264632026709194	24.335879285708604	26.193564375006396	17.205924312575807
4	29.653619371215317	25.547773835763905	25.45853856498317	19.34006822803761
5	26.147525795813785	28.817195011464175	24.41386068157559	20.62141851114645
6	27.578245691414203	33.05319899920086	23.17536606354233	16.1931892458426
7	81.16521938803929	5.257605743795875	8.312634918614023	5.2645399495508105
8	81.09928759561531	4.473244764958844	8.173155074986047	6.254312564439801
9	74.32013522837977	7.374243631614068	10.879655153990523	7.4259659860156395
10	36.5866883711096	30.326351004948293	18.20490464330332	14.882055980638789
11	26.48752922881401	26.019754529116273	27.970312505328536	19.522403736741172
12	24.663719439761643	23.61153897307823	32.00636128121388	19.71838030594625
13	23.16024722148649	25.155479670841206	32.669089470579074	19.015183637093227
14	19.937091975987187	27.264956001896106	30.446164986353256	22.35178703576345
15	19.307102331825618	26.7827445131676	33.54427717725535	20.36587597775143
16	21.947102240885215	26.908924322806605	30.311459513900807	20.832513922407376
17	21.66370920896627	26.65679205453696	28.579044830208584	23.100453906288188
18	22.14671642622403	25.596199600544278	30.987374061750806	21.269709911480884
19	21.867074685943	27.279392790926877	28.723867422532983	22.129665100597137
20	23.498204495411485	28.138779602522234	28.529141283873876	19.8338746181924
21	23.365772533042627	27.88971657286543	29.06819052469202	19.676320369399917
22	21.894470482450206	25.943364590307798	27.69908275235678	24.463082174885216
23	20.2541329571432	27.260408981728933	29.277012425869366	23.208445635258503
24	21.997233138228275	25.078635030016017	29.7030682155333	23.221063616222402
25	19.806478821685193	28.011235686833082	27.635651821024737	24.546633670456988
26	19.970285223207537	27.435241907156673	28.73250676085061	23.861966108785186
27	21.615738146202613	26.31496981346986	29.786165009088357	22.283127031239168
28	18.162844706756985	26.69487334843702	30.596216651869906	24.54606529293609
29	19.706558053511607	25.065789698043755	29.81378815660392	25.413864091840715
30	21.345531472768464	26.329747629013173	29.528121614601393	22.796599283616974
31	19.910150881496698	26.676912618776694	28.49071896346128	24.92221753626533
32	19.291756138761414	26.886984950500004	29.175841227149803	24.64541768358878
33	19.487619032462312	26.67066046604683	30.070808471553274	23.77091202993758
34	18.168983183982668	24.68497675904317	31.085134995344987	26.060905061629175
35	18.01824946544094	24.08965814365628	30.666468113452698	27.225624277450077
36	18.649603215652665	26.637353543322302	31.32362620311312	23.389417037911915
37	18.74838722878446	25.074997413882276	33.207911360388856	22.968703996944402
38	18.40099488801258	26.827873688326775	30.595648274349006	24.17548314931164
39	20.377925581194432	25.138542020718496	30.39455630745586	24.088976090631206
40	20.719861497765706	23.887429421721343	29.9373534296468	25.455355650866153
41	18.23934832106964	24.56504910213403	29.466054789319507	27.729547787476825
42	19.58310645597291	23.296203124484908	32.11389830816747	25.006792111374708
43	20.921067140163032	23.14456000190975	32.35307156896068	23.581301288966543
44	19.785789879924565	24.433299192790244	30.27997139924315	25.500939528042043
45	20.118745431665673	22.933691941657184	29.48935826767626	27.45820435900088
46	20.663251096684426	24.174232718765666	29.595076486562988	25.56743969798692
47	17.46101214395411	24.054305061856525	33.118221387591404	25.36646140659795
48	18.576509866465386	22.810581370631024	32.09173158485251	26.52117717805108
49	20.175924210267855	22.083853872412888	33.39661269732647	24.343609219992793
50	19.47863866763215	21.793526634739006	31.971235550422477	26.756599147206366
51	18.69848368244975	21.367470845075065	29.60371582488061	30.330329647594567
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	101.0
1	323.5
2	546.0
3	957.0
4	1368.0
5	1043.5
6	719.0
7	712.0
8	705.0
9	792.5
10	880.0
11	910.0
12	940.0
13	1004.5
14	1069.0
15	1113.5
16	1158.0
17	1345.0
18	1532.0
19	1617.0
20	1702.0
21	2115.0
22	2528.0
23	2848.5
24	3169.0
25	4363.0
26	6941.0
27	8325.0
28	9649.5
29	10974.0
30	13052.0
31	15130.0
32	17182.0
33	19234.0
34	21341.0
35	23448.0
36	26306.0
37	29164.0
38	32884.5
39	36605.0
40	40241.5
41	43878.0
42	51373.5
43	58869.0
44	71135.5
45	83402.0
46	97315.0
47	111228.0
48	107873.0
49	104518.0
50	89318.0
51	74118.0
52	64176.5
53	54235.0
54	47934.0
55	41633.0
56	37708.5
57	33784.0
58	30994.0
59	28204.0
60	26415.0
61	24626.0
62	21637.0
63	18648.0
64	15893.0
65	13138.0
66	10840.5
67	8543.0
68	7082.0
69	5621.0
70	4532.0
71	3443.0
72	3025.5
73	2608.0
74	2210.0
75	1550.0
76	1288.0
77	937.0
78	586.0
79	480.0
80	374.0
81	267.5
82	161.0
83	139.5
84	118.0
85	62.0
86	6.0
87	3.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	879697.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.690975258038648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.13964694050674	20.605812430845425
2	12.621049841718484	7.747046568453092
3	4.9391000919487515	4.547573961569266
4	2.5749971500611704	3.161166952881896
5	1.589108521550659	2.4385645158624825
6	1.151545066453578	2.120522468582594
7	0.8614987919909238	1.850816667687654
8	0.7735381009841733	1.8992510974764372
9	0.6723277943337953	1.8570956131071188
>10	7.511629943678683	45.55259656910526
>50	0.10751954092780461	2.193335074977987
>100	0.052085578322698604	3.194259824784282
>500	0.0037203984516209574	0.7890021586866217
>1k	0.0018601992258104787	1.4100416291781608
>5k	3.7203984516209575E-4	0.6329144668017286
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	5543	0.6301033196657485	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	3428	0.38967962832657155	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	3152	0.3583051891730903	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	2348	0.26691008381294923	No Hit
TGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT	1985	0.22564587579587062	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1436	0.16323802400144596	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG	897	0.10196692724881408	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3961591320647905	0.0
2	0.0	0.0	0.0	1.8404064126625417	0.0
3	0.0	0.0	0.0	2.5030209265235643	0.0
4	0.0	0.0	0.0	3.3352392926200727	0.0
5	0.0	0.0	0.0	5.498938839168487	0.0
6	0.0	0.0	0.0	6.647061431379213	0.0
7	0.0	0.0	0.0	7.800981474303084	0.0
8	0.0	0.0	0.0	9.654574245450423	0.0
9	0.0	0.0	0.0	10.38721286988588	0.0
10	0.0	0.0	0.0	12.018456354858548	0.0
11	0.0	0.0	0.0	14.277302298405019	0.0
12	0.0	0.0	0.0	15.903771412202156	0.0
13	0.0	0.0	0.0	16.533420029851186	0.0
14	1.1367550417927991E-4	0.0	0.0	16.786348026650085	0.0
15	1.1367550417927991E-4	0.0	0.0	17.282768953401	0.0
16	1.1367550417927991E-4	0.0	0.0	18.325173326724997	0.0
17	1.1367550417927991E-4	0.0	0.0	19.615503974664005	0.0
18	1.1367550417927991E-4	0.0	0.0	20.96767409687654	0.0
19	1.1367550417927991E-4	0.0	0.0	21.81319249696202	0.0
20	2.2735100835855981E-4	0.0	0.0	22.62926894146507	0.0
21	3.410265125378397E-4	0.0	0.0	23.613698807657638	0.0
22	3.410265125378397E-4	0.0	0.0	24.64416725304281	0.0
23	4.5470201671711963E-4	0.0	0.0	25.615524436254756	0.0
24	4.5470201671711963E-4	0.0	0.0	26.36157677018337	0.0
25	5.683775208963996E-4	0.0	0.0	27.065341816557293	0.0
26	5.683775208963996E-4	0.0	0.0	27.699082752356777	0.0
27	5.683775208963996E-4	0.0	0.0	28.29997146744845	0.0
28	5.683775208963996E-4	0.0	0.0	28.91290978598313	0.0
29	5.683775208963996E-4	0.0	0.0	29.544036185186492	0.0
30	5.683775208963996E-4	0.0	0.0	30.267239742775068	0.0
31	5.683775208963996E-4	0.0	0.0	30.91905508373906	0.0
32	6.820530250756794E-4	0.0	0.0	31.56791486159439	0.0
33	6.820530250756794E-4	0.0	0.0	32.186536955338035	0.0
34	6.820530250756794E-4	0.0	0.0	32.7973154392933	0.0
35	6.820530250756794E-4	0.0	0.0	33.42116660622919	0.0
36	6.820530250756794E-4	0.0	0.0	34.00545869771069	0.0
37	6.820530250756794E-4	0.0	0.0	34.61259956553222	0.0
38	6.820530250756794E-4	0.0	0.0	35.19279933886327	0.0
39	6.820530250756794E-4	0.0	0.0	35.80300944529764	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAACG	35	1.2115015E-7	45.000004	1
GGATCGA	25	3.890056E-5	45.0	8
CGTCTAG	20	7.032383E-4	45.0	29
TAACGCG	25	3.890056E-5	45.0	1
TAACGAC	20	7.032383E-4	45.0	11
TTATCCG	20	7.032383E-4	45.0	1
TCGGCGT	50	2.1827873E-11	45.0	4
ATCGTAA	20	7.032383E-4	45.0	20
TACGCTA	20	7.032383E-4	45.0	34
CGCTACA	40	6.8121153E-9	45.0	28
TTGCGAG	45	3.8380676E-10	45.0	1
CGACTCG	20	7.032383E-4	45.0	2
TCGACAC	25	3.890056E-5	45.0	34
ATCATCG	20	7.032383E-4	45.0	17
GCCGTAC	20	7.032383E-4	45.0	27
TGCGTGA	20	7.032383E-4	45.0	38
ACGCGTA	20	7.032383E-4	45.0	23
ATCACGG	70	0.0	41.785717	2
GCAACGA	60	3.6379788E-12	41.249996	11
CGCGCAA	60	3.6379788E-12	41.249996	18
>>END_MODULE