SRR3552493_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552493_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	330877
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1976	0.5972007724924973	Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	1565	0.4729854296309505	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1333	0.4028687397431674	Illumina PCR Primer Index 8 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1013	0.3061560640358804	Illumina PCR Primer Index 8 (95% over 23bp)
TGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT	817	0.24691955016516712	Illumina PCR Primer Index 8 (96% over 25bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	652	0.19705207675359726	No Hit
ACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	577	0.17438504338470187	Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC	493	0.14899796601153903	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	443	0.13388661043227543	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	384	0.11605521084874439	No Hit
GCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG	383	0.11575298373715914	Illumina PCR Primer Index 8 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	360	0.10880176017069787	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAC	80	0.0	45.0	5
AGGTACA	20	7.0259965E-4	45.0	6
ACGCATT	20	7.0259965E-4	45.0	17
CAATTGG	20	7.0259965E-4	45.0	2
GCGTTAG	35	1.2084274E-7	45.0	1
GACTCAT	20	7.0259965E-4	45.0	9
GACTACC	20	7.0259965E-4	45.0	38
TCGCAGG	55	1.8189894E-12	44.999996	2
GGTGTAC	25	3.8847647E-5	44.999996	8
CGATGCA	25	3.8847647E-5	44.999996	10
ATAGTAG	25	3.8847647E-5	44.999996	1
TATCGGG	25	3.8847647E-5	44.999996	1
GCGTAAG	30	2.1607993E-6	44.999996	1
ACATACG	80	0.0	42.1875	17
TATGGGC	135	0.0	41.666668	4
ATAGGGC	215	0.0	40.813957	4
CGAAAGG	50	1.0768417E-9	40.499996	2
TACGAAT	50	1.0768417E-9	40.499996	12
CGAATAT	50	1.0768417E-9	40.499996	14
GCTACGA	50	1.0768417E-9	40.499996	10