##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552493_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	330877
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52728959704059	34.0	31.0	34.0	31.0	34.0
2	32.68413640114	34.0	31.0	34.0	31.0	34.0
3	32.720367387276845	34.0	31.0	34.0	31.0	34.0
4	36.12984281167927	37.0	37.0	37.0	35.0	37.0
5	36.16228386983683	37.0	35.0	37.0	35.0	37.0
6	36.207575624779	37.0	36.0	37.0	35.0	37.0
7	36.265690876065726	37.0	37.0	37.0	35.0	37.0
8	36.25811404237829	37.0	37.0	37.0	35.0	37.0
9	37.744657380234955	39.0	38.0	39.0	35.0	39.0
10	37.837277900851376	39.0	38.0	39.0	35.0	39.0
11	37.809802434137154	39.0	37.0	39.0	35.0	39.0
12	37.67639334254119	39.0	37.0	39.0	35.0	39.0
13	37.639651592585764	39.0	37.0	39.0	35.0	39.0
14	38.94819525080317	40.0	38.0	41.0	35.0	41.0
15	39.00174082816273	40.0	38.0	41.0	35.0	41.0
16	39.04604430045001	40.0	38.0	41.0	35.0	41.0
17	39.04879758943656	40.0	38.0	41.0	35.0	41.0
18	39.0566222493555	40.0	38.0	41.0	35.0	41.0
19	39.0306186286747	40.0	38.0	41.0	35.0	41.0
20	38.99549077149515	40.0	38.0	41.0	35.0	41.0
21	38.909102173919614	40.0	38.0	41.0	35.0	41.0
22	38.91505000347561	40.0	38.0	41.0	35.0	41.0
23	38.89508790275541	40.0	38.0	41.0	35.0	41.0
24	38.8704171036367	40.0	38.0	41.0	35.0	41.0
25	38.82140493295091	40.0	38.0	41.0	35.0	41.0
26	38.78536132762326	40.0	38.0	41.0	35.0	41.0
27	38.75893761125736	40.0	38.0	41.0	35.0	41.0
28	38.69982198823128	40.0	38.0	41.0	35.0	41.0
29	38.657059874213076	40.0	38.0	41.0	35.0	41.0
30	38.56894555983039	40.0	38.0	41.0	35.0	41.0
31	38.49702155181533	40.0	38.0	41.0	35.0	41.0
32	38.40470325831049	40.0	37.0	41.0	35.0	41.0
33	38.292008812942576	40.0	37.0	41.0	35.0	41.0
34	38.21240219175101	40.0	37.0	41.0	34.0	41.0
35	38.15330168008051	40.0	37.0	41.0	34.0	41.0
36	38.10502392127588	40.0	37.0	41.0	34.0	41.0
37	38.05477564170371	40.0	37.0	41.0	34.0	41.0
38	37.98990561447305	40.0	37.0	41.0	34.0	41.0
39	37.92316782369279	40.0	36.0	41.0	34.0	41.0
40	37.83181665694503	40.0	36.0	41.0	34.0	41.0
41	37.80544129691698	40.0	36.0	41.0	34.0	41.0
42	37.788558890463825	40.0	36.0	41.0	34.0	41.0
43	37.747534582337245	40.0	36.0	41.0	34.0	41.0
44	37.659961254484294	40.0	35.0	41.0	34.0	41.0
45	37.59248905182288	40.0	35.0	41.0	33.0	41.0
46	37.46583473617084	40.0	35.0	41.0	33.0	41.0
47	37.40090728578898	39.0	35.0	41.0	33.0	41.0
48	37.36021844975625	39.0	35.0	41.0	33.0	41.0
49	37.32271206520852	39.0	35.0	41.0	33.0	41.0
50	37.236906765958345	39.0	35.0	41.0	33.0	41.0
51	36.65198548100956	39.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	4.0
15	6.0
16	12.0
17	21.0
18	30.0
19	58.0
20	109.0
21	160.0
22	232.0
23	458.0
24	827.0
25	1203.0
26	1407.0
27	1616.0
28	1894.0
29	2270.0
30	2715.0
31	3344.0
32	4595.0
33	6756.0
34	14699.0
35	25670.0
36	19285.0
37	30684.0
38	58301.0
39	154042.0
40	475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.27938780876278	21.07973657884954	26.53674930563321	11.104126306754473
2	31.790060959208404	24.90472290307274	25.062787682431843	18.242428455287012
3	31.835092798834612	23.870199500116357	26.859225633694695	17.435482067354336
4	30.081268870305276	25.469887601737202	26.041701296856534	18.407142231100984
5	25.951637617604124	28.986602272143426	24.253725704718068	20.808034405534386
6	26.68574727164475	33.487670644982884	23.28901676453788	16.537565318834492
7	77.64033160358683	7.178196127261792	9.493255802004974	5.688216467146401
8	77.88241551996663	6.2146960955279456	9.033266138172191	6.869622246333229
9	71.32590056123574	8.200025991531597	12.14439202483098	8.329681422401677
10	40.797637792895856	23.477002027943918	19.19081713144159	16.53454304771864
11	29.94254662608764	25.674797583392017	23.14969006609707	21.232965724423273
12	26.573016559023444	22.405909144485715	30.07431764673882	20.94675664975202
13	25.412162223424417	24.869362331017268	30.288294441741193	19.43018100381713
14	19.843023238242612	27.22975607249824	29.573225095730436	23.353995593528712
15	18.9608222995252	25.55300005742315	33.91229973676019	21.57387790629146
16	20.346231379032087	25.486207865762804	30.751910830912998	23.415649924292108
17	20.73459321741916	24.846997524759956	28.532052696319177	25.886356561501707
18	20.531798825545444	24.80196568513375	30.871290539989182	23.794944949331626
19	22.551280385158233	26.78034435757094	28.139157451258324	22.529217806012504
20	23.904955617948666	27.30349948772505	28.170891297974777	20.620653596351517
21	22.285924981186362	28.09503229296687	28.77413661269898	20.844906113147786
22	20.86575978384717	26.85257663723982	26.514686726487486	25.766976852425522
23	20.889333498550823	26.41162728143691	27.898282443324863	24.80075677668741
24	21.63523000994327	25.329654221961633	28.47281618244846	24.56229958564663
25	20.577132892283235	27.75200452131759	26.06406610311385	25.606796483285333
26	19.887752850757227	28.88021832886541	27.595753104628002	23.63627571574936
27	23.160872469225723	27.002179057474528	27.13636789501839	22.700580578281354
28	21.01929115653249	27.60905109753776	27.52261414362437	23.84904360230539
29	23.30412812011714	27.207391266240926	25.864596209467567	23.623884404174362
30	25.079712400680616	28.121930505897964	24.46468022860459	22.333676864816834
31	25.263164257412875	29.819540191672434	21.899376505468798	23.017919045445893
32	26.333954913759495	29.455356522212185	21.961635290455366	22.249053273572958
33	25.49436799777561	28.205949642918664	22.16473190944067	24.134950449865055
34	23.91160461440354	28.452264738860666	24.396376901386315	23.239753745349482
35	25.17733175772266	27.408976749668305	23.394796253592727	24.01889523901631
36	26.46149475484848	29.558718194374343	22.216412745521747	21.76337430525543
37	27.710901634141994	28.042142548439454	24.165777615246753	20.081178202171802
38	27.228547164051896	28.28996877993937	22.34939267461927	22.13209138138946
39	25.116886335405603	27.3721050420549	24.200231505967473	23.31077711657202
40	24.86180665322763	27.370593906496975	26.047443611976657	21.720155828298733
41	23.104658226470864	27.426505922140255	23.907977889064515	25.56085796232437
42	24.34499829241682	24.626371733302708	25.856133850343177	25.172496123937293
43	25.917788181106577	23.24882055869704	26.164405504160154	24.66898575603623
44	24.24224107447783	24.386403406704	25.763047899974918	25.608307618843256
45	24.709484188988657	24.153084076560173	25.119304152298287	26.018127582152882
46	24.903211767514815	26.57664328436247	25.591685127706064	22.92845982041665
47	22.11667779869861	25.974002423861435	28.96786419122514	22.94145558621482
48	23.38361385046407	23.64957370865911	27.651967347382865	25.31484509349396
49	23.88017299479867	22.813915745125833	29.08240826651596	24.22350299355954
50	22.4554743907857	22.847462954511798	28.2237810425022	26.473281612200307
51	21.893634190348678	22.286831662521116	25.64880605179568	30.17072809533452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	211.5
2	335.0
3	634.0
4	933.0
5	678.0
6	423.0
7	452.0
8	481.0
9	508.5
10	536.0
11	598.0
12	660.0
13	614.5
14	569.0
15	592.0
16	615.0
17	633.5
18	652.0
19	648.5
20	645.0
21	777.5
22	910.0
23	951.0
24	992.0
25	1683.0
26	2895.5
27	3417.0
28	3984.5
29	4552.0
30	5029.5
31	5507.0
32	5716.0
33	5925.0
34	6578.5
35	7232.0
36	7791.0
37	8350.0
38	8913.0
39	9476.0
40	10319.0
41	11162.0
42	12402.5
43	13643.0
44	15203.5
45	16764.0
46	18486.5
47	20209.0
48	24678.5
49	29148.0
50	34191.5
51	39235.0
52	39351.5
53	39468.0
54	33634.0
55	27800.0
56	23327.5
57	18855.0
58	16337.5
59	13820.0
60	13154.5
61	12489.0
62	11152.5
63	9816.0
64	8962.5
65	8109.0
66	6542.5
67	4976.0
68	4149.0
69	3322.0
70	3023.0
71	2724.0
72	2326.5
73	1929.0
74	1500.5
75	941.5
76	811.0
77	629.5
78	448.0
79	339.5
80	231.0
81	171.0
82	111.0
83	78.5
84	46.0
85	29.5
86	13.0
87	8.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	330877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.93142280987357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.48057989272512	25.290852502835577
2	12.643957369625863	9.339186712153392
3	4.841232754107934	5.363808412889064
4	2.629232377856099	3.8840517044805143
5	1.7216471991065696	3.1791440319819646
6	1.2679408509762282	2.809611579918839
7	1.026783246589566	2.654439634972576
8	0.8800587767880755	2.6001458226480456
9	0.7473037184168303	2.483909063301839
>10	5.64326895294603	35.19462923932051
>50	0.06964974425864076	1.8024006235440797
>100	0.04260925531116847	2.997141668163292
>500	0.002458226267952027	0.6191591127889837
>1k	0.003277635023936036	1.7815198910013426
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1976	0.5972007724924973	Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	1565	0.4729854296309505	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1333	0.4028687397431674	Illumina PCR Primer Index 8 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1013	0.3061560640358804	Illumina PCR Primer Index 8 (95% over 23bp)
TGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT	817	0.24691955016516712	Illumina PCR Primer Index 8 (96% over 25bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	652	0.19705207675359726	No Hit
ACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	577	0.17438504338470187	Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC	493	0.14899796601153903	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	443	0.13388661043227543	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	384	0.11605521084874439	No Hit
GCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG	383	0.11575298373715914	Illumina PCR Primer Index 8 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	360	0.10880176017069787	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.483563378536435	0.0
2	0.0	0.0	0.0	2.0004412515829144	0.0
3	0.0	0.0	0.0	2.681358933984532	0.0
4	0.0	0.0	0.0	3.5233636668610995	0.0
5	0.0	0.0	0.0	5.653158122202511	0.0
6	0.0	0.0	0.0	6.836679491170435	0.0
7	0.0	0.0	0.0	8.039543395279816	0.0
8	0.0	0.0	0.0	9.911840351550577	0.0
9	0.0	0.0	0.0	10.714555559921058	0.0
10	0.0	0.0	0.0	12.690516415465567	0.0
11	0.0	0.0	0.0	15.64780870232745	0.0
12	0.0	0.0	0.0	17.908467496985285	0.0
13	0.0	0.0	0.0	18.76225908721368	0.0
14	0.0	0.0	0.0	19.140345203806852	0.0
15	0.0	0.0	0.0	19.75930632833349	0.0
16	0.0	0.0	0.0	21.108750381561727	0.0
17	3.0222711158527186E-4	0.0	0.0	22.620792620822844	0.0
18	3.0222711158527186E-4	0.0	0.0	24.351042834648524	0.0
19	3.0222711158527186E-4	0.0	0.0	25.315449547717126	0.0
20	3.0222711158527186E-4	0.0	0.0	26.254771410524153	0.0
21	6.044542231705437E-4	0.0	0.0	27.305615077506143	0.0
22	6.044542231705437E-4	0.0	0.0	28.35283201914911	0.0
23	6.044542231705437E-4	0.0	0.0	29.40065341501525	0.0
24	0.0012089084463410874	0.0	0.0	30.234800242990598	0.0
25	0.0012089084463410874	0.0	0.0	30.97737225615561	0.0
26	0.0012089084463410874	0.0	0.0	31.624742729171263	0.0
27	0.0012089084463410874	0.0	0.0	32.27332211063326	0.0
28	0.0012089084463410874	0.0	0.0	32.897118868945256	0.0
29	0.0012089084463410874	0.0	0.0	33.54600047751884	0.0
30	0.0012089084463410874	0.0	0.0	34.25018964751252	0.0
31	0.0012089084463410874	0.0	0.0	34.929293967244625	0.0
32	0.0012089084463410874	0.0	0.0	35.584522345161496	0.0
33	0.0012089084463410874	0.0	0.0	36.19925229012594	0.0
34	0.0012089084463410874	0.0	0.0	36.79222188305624	0.0
35	0.0015111355579263594	0.0	0.0	37.4066496009091	0.0
36	0.0015111355579263594	0.0	0.0	38.00747709874062	0.0
37	0.0015111355579263594	0.0	0.0	38.61858031836604	0.0
38	0.0015111355579263594	0.0	0.0	39.244190439347555	0.0
39	0.0015111355579263594	0.0	0.0	39.85317806919188	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAC	80	0.0	45.0	5
AGGTACA	20	7.0259965E-4	45.0	6
ACGCATT	20	7.0259965E-4	45.0	17
CAATTGG	20	7.0259965E-4	45.0	2
GCGTTAG	35	1.2084274E-7	45.0	1
GACTCAT	20	7.0259965E-4	45.0	9
GACTACC	20	7.0259965E-4	45.0	38
TCGCAGG	55	1.8189894E-12	44.999996	2
GGTGTAC	25	3.8847647E-5	44.999996	8
CGATGCA	25	3.8847647E-5	44.999996	10
ATAGTAG	25	3.8847647E-5	44.999996	1
TATCGGG	25	3.8847647E-5	44.999996	1
GCGTAAG	30	2.1607993E-6	44.999996	1
ACATACG	80	0.0	42.1875	17
TATGGGC	135	0.0	41.666668	4
ATAGGGC	215	0.0	40.813957	4
CGAAAGG	50	1.0768417E-9	40.499996	2
TACGAAT	50	1.0768417E-9	40.499996	12
CGAATAT	50	1.0768417E-9	40.499996	14
GCTACGA	50	1.0768417E-9	40.499996	10
>>END_MODULE