FastQCFastQC Report
Sat 18 Jun 2016
SRR3552492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434030
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC41840.9639886643780383Illumina PCR Primer Index 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC32710.753634541391148RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT32300.7441881897564684TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC18990.43752735985991753Illumina PCR Primer Index 2 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT18520.42669861530308967Illumina PCR Primer Index 2 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC12770.2942192935972168TruSeq Adapter, Index 10 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA8150.18777503859180242No Hit
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG7910.18224546690320945Illumina PCR Primer Index 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC6980.16081837660991175No Hit
ACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG6870.1582839895859733TruSeq Adapter, Index 10 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC6530.15045042969379996No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG6530.15045042969379996No Hit
GGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG6380.14699444738842937RNA PCR Primer, Index 10 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG5720.13178812524479874No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5570.12833214293942816No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC5000.11519941017901988No Hit
GGCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT4970.11450821371794577TruSeq Adapter, Index 10 (95% over 21bp)
AAGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC4890.11266502315508144No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4840.11151302905329123No Hit
AGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG4790.11036103495150106TruSeq Adapter, Index 10 (95% over 22bp)
GACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT4480.10321867152040182RNA PCR Primer, Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG207.02843E-445.01
GATACGC207.02843E-445.010
TTGGCCG253.88678E-545.01
TCGCAAG253.88678E-545.01
TTAATCG253.88678E-545.020
GGCGTCA207.02843E-445.036
GCGTTAG406.7975634E-945.01
TCGGCGT900.045.04
ACGATTA253.88678E-545.014
AATGCGG351.2095916E-745.02
TCTACGG406.7975634E-945.02
CAAGGCG207.02843E-445.01
ACGTGAT253.88678E-545.028
GTCAATA253.88678E-545.09
GAACGGT207.02843E-445.012
TCTCAAC253.88678E-545.035
ATAACGG207.02843E-445.02
ATACGCA207.02843E-445.011
GCGTCAT253.88678E-545.037
AATACGG351.2095916E-745.02
Produced by FastQC (version 0.11.5)