##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552492_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434030
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49553947883787	34.0	31.0	34.0	31.0	34.0
2	32.64789761076423	34.0	31.0	34.0	31.0	34.0
3	32.68711840195378	34.0	31.0	34.0	31.0	34.0
4	36.10925972859019	37.0	35.0	37.0	35.0	37.0
5	36.15700988410939	37.0	35.0	37.0	35.0	37.0
6	36.1923438471995	37.0	36.0	37.0	35.0	37.0
7	36.27071170195609	37.0	37.0	37.0	35.0	37.0
8	36.25808123862406	37.0	37.0	37.0	35.0	37.0
9	37.748418312098245	39.0	38.0	39.0	35.0	39.0
10	37.82523788678202	39.0	38.0	39.0	35.0	39.0
11	37.79243600672765	39.0	37.0	39.0	35.0	39.0
12	37.69757850839804	39.0	37.0	39.0	35.0	39.0
13	37.66024007557081	39.0	37.0	39.0	35.0	39.0
14	38.94931456350943	40.0	38.0	41.0	35.0	41.0
15	38.99420316567979	40.0	38.0	41.0	35.0	41.0
16	39.04453609197521	40.0	38.0	41.0	35.0	41.0
17	39.052611570628756	40.0	38.0	41.0	35.0	41.0
18	39.058583508052436	40.0	38.0	41.0	35.0	41.0
19	39.04922470796949	40.0	38.0	41.0	35.0	41.0
20	39.0081077344884	40.0	38.0	41.0	35.0	41.0
21	38.94835840840495	40.0	38.0	41.0	35.0	41.0
22	38.96398405640163	40.0	38.0	41.0	35.0	41.0
23	38.938220860309194	40.0	38.0	41.0	35.0	41.0
24	38.916874409603025	40.0	38.0	41.0	35.0	41.0
25	38.87521139091768	40.0	38.0	41.0	35.0	41.0
26	38.83992350759164	40.0	38.0	41.0	35.0	41.0
27	38.819968665760435	40.0	38.0	41.0	35.0	41.0
28	38.77954519272861	40.0	38.0	41.0	35.0	41.0
29	38.75420593046564	40.0	38.0	41.0	35.0	41.0
30	38.67986544708891	40.0	38.0	41.0	35.0	41.0
31	38.603656429279084	40.0	38.0	41.0	35.0	41.0
32	38.54217220007833	40.0	38.0	41.0	35.0	41.0
33	38.46707370458263	40.0	38.0	41.0	35.0	41.0
34	38.372278413934524	40.0	38.0	41.0	35.0	41.0
35	38.33535930696035	40.0	38.0	41.0	35.0	41.0
36	38.293585696841234	40.0	37.0	41.0	35.0	41.0
37	38.23002327028085	40.0	37.0	41.0	34.0	41.0
38	38.20451120890261	40.0	37.0	41.0	34.0	41.0
39	38.15930696034836	40.0	37.0	41.0	34.0	41.0
40	38.09885261387462	40.0	37.0	41.0	34.0	41.0
41	38.05454461673156	40.0	37.0	41.0	34.0	41.0
42	38.01440453424878	40.0	37.0	41.0	34.0	41.0
43	37.98066953897196	40.0	36.0	41.0	34.0	41.0
44	37.91599659009746	40.0	36.0	41.0	34.0	41.0
45	37.89971660945096	40.0	36.0	41.0	34.0	41.0
46	37.805607907287516	40.0	36.0	41.0	34.0	41.0
47	37.73102550514941	40.0	36.0	41.0	34.0	41.0
48	37.69988249660162	40.0	35.0	41.0	34.0	41.0
49	37.698536967490725	40.0	35.0	41.0	34.0	41.0
50	37.6303734764878	40.0	35.0	41.0	34.0	41.0
51	37.066101421560724	39.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	1.0
13	1.0
14	4.0
15	4.0
16	19.0
17	19.0
18	40.0
19	71.0
20	116.0
21	183.0
22	365.0
23	598.0
24	869.0
25	1344.0
26	1655.0
27	1930.0
28	2207.0
29	2552.0
30	3272.0
31	4168.0
32	5917.0
33	8700.0
34	18297.0
35	31217.0
36	24129.0
37	38875.0
38	74018.0
39	212912.0
40	545.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.54374121604498	21.2953021680529	25.628182383706193	12.532774232195932
2	30.70525078911596	25.196875791995943	25.20793493537313	18.889938483514964
3	31.510264267446953	23.48869893786144	27.286823491463725	17.714213303227886
4	29.8336520517015	24.895974932608343	26.065479344745757	19.204893670944404
5	25.110937032002393	27.60039628597102	25.87171393682464	21.416952745201947
6	26.527659378383984	32.40628527981937	23.861714628021105	17.204340713775544
7	75.08167638181692	6.476050042623783	11.669700251134714	6.772573324424578
8	73.35414602677234	6.102343156003041	11.416031149920512	9.127479667304103
9	67.31124576642168	8.133769555099878	14.561896643089186	9.993088035389258
10	38.47107342810405	21.95562518719904	22.377255028454254	17.196046356242654
11	27.538649402115063	24.834688846393107	25.44063774393475	22.18602400755708
12	24.311453125359996	21.35382346842384	32.681381471326866	21.653341934889294
13	22.92306983388245	24.16883625555837	32.8986475589245	20.00944635163468
14	18.781420639126328	26.910582217819044	29.978112112065986	24.329885030988642
15	17.513305531875677	25.713890744879386	35.337649471234705	21.43515425201023
16	18.351496440338224	25.993594912794048	31.44943897887243	24.2054696679953
17	18.688569914522038	25.640623920005527	28.33836370757782	27.33244245789462
18	18.72589452342004	25.349399811072963	32.73276040826671	23.19194525724028
19	20.495357463769786	26.156026081146464	29.35764808884179	23.990968366241965
20	21.16374444162846	27.71974287491648	29.541275948667145	21.575236734787918
21	21.06259935949128	27.508697555468515	29.76729719143838	21.661405893601827
22	18.970117272999563	26.66658986705988	26.96034836301638	27.402944496924174
23	19.203741676842615	25.749602562034884	28.527521139091768	26.519134622030737
24	21.890652719858075	24.31352671474322	28.277768817823652	25.518051747575054
25	19.322397069327003	26.797686795843607	26.567287975485566	27.312628159343827
26	18.7261249222404	26.77856369375389	30.070502039029563	24.424809344976154
27	23.92092712485312	25.373822086030916	27.793240098610696	22.912010690505262
28	17.79508328917356	27.350643964702904	29.45464599221252	25.39962675391102
29	20.46747920650646	24.313065917102506	31.52224500610557	23.697209870285462
30	23.39216183213142	25.72518028707693	28.17570214040504	22.706955740386608
31	20.3409902541299	30.84325968251043	24.925235582793817	23.89051448056586
32	20.28200815611824	26.737783102550516	28.01373176969334	24.966476971637906
33	21.620164504757735	25.126373752966387	27.406630878049903	25.84683086422597
34	18.840633135958342	26.455774946432275	30.152063221436304	24.551528696173076
35	19.894707739096376	22.600281086560837	30.31219040158514	27.192820772757642
36	22.059765454000875	26.13137340736815	28.080317028776815	23.72854410985416
37	20.290302513651127	27.875722876298877	29.952077045365527	21.88189756468447
38	18.69870746261779	26.746538257724122	28.30587747390733	26.248876805750754
39	20.744648987397184	23.556896988687416	30.90155058406101	24.796903439854386
40	20.945556758749394	23.500910075340414	31.127802225652605	24.425730940257587
41	19.483445844757274	24.236803907563996	26.295878165103794	29.983872082574937
42	20.652719858074324	22.032808792018983	29.204432873303688	28.110038476603
43	23.282491993640992	21.41603114992051	29.569845402391543	25.731631454046955
44	20.715388337211714	22.794276893302307	29.699329539432757	26.791005230053223
45	21.453355758818514	20.569545883925073	30.20436375365758	27.77273460359883
46	22.71386770499735	22.464345782549593	30.473008778195055	24.348777734258
47	18.002672626316155	23.33709651406585	33.513582010460105	25.14664884915789
48	19.068267170472087	21.377093749280004	30.28592493606433	29.268714144183583
49	21.106375135359308	19.468239522613644	33.29170794645531	26.133677395571738
50	19.811303366126765	19.16503467502246	32.57655000806396	28.447111950786812
51	19.259728590189617	19.162730686818882	28.492500518397346	33.08504020459415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	147.0
1	250.0
2	353.0
3	681.5
4	1010.0
5	729.5
6	449.0
7	511.5
8	574.0
9	616.0
10	658.0
11	706.5
12	755.0
13	714.0
14	673.0
15	692.5
16	712.0
17	728.0
18	744.0
19	805.0
20	866.0
21	983.5
22	1101.0
23	1189.5
24	1278.0
25	1830.0
26	3123.0
27	3864.0
28	4138.5
29	4413.0
30	5328.0
31	6243.0
32	6958.0
33	7673.0
34	8079.0
35	8485.0
36	9382.0
37	10279.0
38	11208.5
39	12138.0
40	14888.0
41	17638.0
42	21597.5
43	25557.0
44	34998.0
45	44439.0
46	53263.0
47	62087.0
48	60185.5
49	58284.0
50	47262.5
51	36241.0
52	30271.0
53	24301.0
54	21181.5
55	18062.0
56	17044.0
57	16026.0
58	15037.5
59	14049.0
60	13792.5
61	13536.0
62	12340.5
63	11145.0
64	10050.5
65	8956.0
66	7372.0
67	5788.0
68	4942.5
69	4097.0
70	3768.5
71	3440.0
72	2897.0
73	2354.0
74	1949.5
75	1209.0
76	873.0
77	648.5
78	424.0
79	309.0
80	194.0
81	154.0
82	114.0
83	77.0
84	40.0
85	33.5
86	27.0
87	21.5
88	16.0
89	8.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434030.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.80004509868736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.18978737739084	23.382076309044166
2	13.382660123898631	9.314343517041548
3	5.164924843519011	5.392188524573771
4	2.816284218197633	3.920272712159964
5	1.7736765762329996	3.086201242169689
6	1.2930989210121866	2.699994046097284
7	1.0387363287898426	2.5303649761272027
8	0.8598196853368066	2.3937411061168037
9	0.7567792779259899	2.3702357701418855
>10	5.559727093298614	33.32755558171096
>50	0.0922027099243532	2.1989698218809908
>100	0.06235291174740432	4.3570811870673625
>500	0.005969959635389775	1.3998061095828602
>1k	0.00397997309025985	3.6271690962855345
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	4184	0.9639886643780383	Illumina PCR Primer Index 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	3271	0.753634541391148	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	3230	0.7441881897564684	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1899	0.43752735985991753	Illumina PCR Primer Index 2 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT	1852	0.42669861530308967	Illumina PCR Primer Index 2 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1277	0.2942192935972168	TruSeq Adapter, Index 10 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	815	0.18777503859180242	No Hit
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	791	0.18224546690320945	Illumina PCR Primer Index 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC	698	0.16081837660991175	No Hit
ACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	687	0.1582839895859733	TruSeq Adapter, Index 10 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	653	0.15045042969379996	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	653	0.15045042969379996	No Hit
GGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	638	0.14699444738842937	RNA PCR Primer, Index 10 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	572	0.13178812524479874	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	557	0.12833214293942816	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	500	0.11519941017901988	No Hit
GGCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	497	0.11450821371794577	TruSeq Adapter, Index 10 (95% over 21bp)
AAGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	489	0.11266502315508144	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	484	0.11151302905329123	No Hit
AGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	479	0.11036103495150106	TruSeq Adapter, Index 10 (95% over 22bp)
GACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	448	0.10321867152040182	RNA PCR Primer, Index 10 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7603161071815312	0.0
2	0.0	0.0	0.0	3.250927355251941	0.0
3	0.0	0.0	0.0	4.329885030988641	0.0
4	0.0	0.0	0.0	5.600073727622514	0.0
5	0.0	0.0	0.0	8.944773402760179	0.0
6	0.0	0.0	0.0	10.615164850355967	0.0
7	0.0	0.0	0.0	12.412275649148677	0.0
8	0.0	0.0	0.0	15.076838006589407	0.0
9	0.0	0.0	0.0	16.161094855194342	0.0
10	0.0	0.0	0.0	18.670138008893396	0.0
11	0.0	0.0	0.0	22.011381701725686	0.0
12	0.0	0.0	0.0	24.440246065940144	0.0
13	0.0	0.0	0.0	25.383038038845243	0.0
14	0.0	0.0	0.0	25.750063359675597	0.0
15	0.0	0.0	0.0	26.435730249061123	0.0
16	0.0	0.0	0.0	27.782411354053867	0.0
17	0.0	0.0	0.0	29.4426652535539	0.0
18	0.0	0.0	0.0	31.307743704352234	0.0
19	2.3039882035803976E-4	0.0	0.0	32.411123655046886	0.0
20	2.3039882035803976E-4	0.0	0.0	33.38386747459853	0.0
21	2.3039882035803976E-4	0.0	0.0	34.53678317167016	0.0
22	2.3039882035803976E-4	0.0	0.0	35.66619818906527	0.0
23	2.3039882035803976E-4	0.0	0.0	36.75345022233486	0.0
24	2.3039882035803976E-4	0.0	0.0	37.60661705412068	0.0
25	4.607976407160795E-4	0.0	0.0	38.34112849342211	0.0
26	6.911964610741192E-4	0.0	0.0	39.0341681450591	0.0
27	9.21595281432159E-4	0.0	0.0	39.69979033707347	0.0
28	9.21595281432159E-4	0.0	0.0	40.39213879224938	0.0
29	9.21595281432159E-4	0.0	0.0	41.06213856185056	0.0
30	9.21595281432159E-4	0.0	0.0	41.75725180287077	0.0
31	9.21595281432159E-4	0.0	0.0	42.438771513489854	0.0
32	0.0011519941017901988	0.0	0.0	43.09010897864203	0.0
33	0.0011519941017901988	0.0	0.0	43.67969955993825	0.0
34	0.0011519941017901988	0.0	0.0	44.28657005276133	0.0
35	0.0013823929221482385	0.0	0.0	44.89850931963228	0.0
36	0.0016127917425062784	0.0	0.0	45.51713015229362	0.0
37	0.0016127917425062784	0.0	0.0	46.08068566688939	0.0
38	0.0016127917425062784	0.0	0.0	46.68893855263461	0.0
39	0.001843190562864318	0.0	0.0	47.2732299610626	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	20	7.02843E-4	45.0	1
GATACGC	20	7.02843E-4	45.0	10
TTGGCCG	25	3.88678E-5	45.0	1
TCGCAAG	25	3.88678E-5	45.0	1
TTAATCG	25	3.88678E-5	45.0	20
GGCGTCA	20	7.02843E-4	45.0	36
GCGTTAG	40	6.7975634E-9	45.0	1
TCGGCGT	90	0.0	45.0	4
ACGATTA	25	3.88678E-5	45.0	14
AATGCGG	35	1.2095916E-7	45.0	2
TCTACGG	40	6.7975634E-9	45.0	2
CAAGGCG	20	7.02843E-4	45.0	1
ACGTGAT	25	3.88678E-5	45.0	28
GTCAATA	25	3.88678E-5	45.0	9
GAACGGT	20	7.02843E-4	45.0	12
TCTCAAC	25	3.88678E-5	45.0	35
ATAACGG	20	7.02843E-4	45.0	2
ATACGCA	20	7.02843E-4	45.0	11
GCGTCAT	25	3.88678E-5	45.0	37
AATACGG	35	1.2095916E-7	45.0	2
>>END_MODULE