SRR3552487_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552487_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	420843
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	4207	0.9996602058249751	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	3557	0.8452083080863886	Illumina PCR Primer Index 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	3027	0.7192706068533872	TruSeq Adapter, Index 13 (95% over 22bp)
TGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCTT	2087	0.49590940089296964	TruSeq Adapter, Index 13 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	2036	0.4837908673780959	TruSeq Adapter, Index 13 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1646	0.39111972873494394	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC	1379	0.32767564151001677	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	898	0.2133812371834627	No Hit
GCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	845	0.20078746706016257	TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	804	0.19104511658742096	No Hit
AGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	659	0.1565904624765055	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	635	0.15088762317538845	No Hit
ACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	632	0.1501747682627488	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	600	0.1425709825279261	No Hit
CGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	553	0.1314029222299052	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	465	0.11049251145914271	No Hit
GGCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	463	0.11001727485071632	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	456	0.10835394672122382	No Hit
GGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	453	0.10764109180858421	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	452	0.10740347350437099	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTC	442	0.10502729046223888	No Hit
GGAGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	424	0.10075016098640112	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGCG	70	0.0	45.000004	1
CGGGTAC	20	7.0281845E-4	45.0	5
CAACGGA	20	7.0281845E-4	45.0	3
CGTGCTC	25	3.886577E-5	45.0	10
GAATGCG	25	3.886577E-5	45.0	1
GGCCGTG	25	3.886577E-5	45.0	7
CACGAGG	25	3.886577E-5	45.0	2
ACGGCAT	25	3.886577E-5	45.0	5
GTATGCG	25	3.886577E-5	45.0	1
ACGGAAC	20	7.0281845E-4	45.0	5
CGGCTTG	20	7.0281845E-4	45.0	6
TATAGCG	40	6.7975634E-9	45.0	1
ATGCGAG	20	7.0281845E-4	45.0	1
TTACGCG	20	7.0281845E-4	45.0	1
GCAATTT	20	7.0281845E-4	45.0	8
ACCCGAT	20	7.0281845E-4	45.0	38
TTGCACG	20	7.0281845E-4	45.0	1
GCCGTGC	25	3.886577E-5	45.0	8
CACGGAC	30	2.1622109E-6	44.999996	4
TAGCGAG	30	2.1622109E-6	44.999996	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content