FastQCFastQC Report
Sat 18 Jun 2016
SRR3552483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438370
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC72971.6645755868330405TruSeq Adapter, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC61981.4138741245979425TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT56541.2897780413805688TruSeq Adapter, Index 27 (100% over 25bp)
TGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT36880.8412984465177819TruSeq Adapter, Index 27 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC35380.8070807765129914TruSeq Adapter, Index 27 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC28710.6549262038916897TruSeq Adapter, Index 27 (100% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC16990.3875721422542601TruSeq Adapter, Index 27 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG16040.3659009512512261No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG15440.3522138832493099TruSeq Adapter, Index 27 (100% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG14280.3257522184456053TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC13050.2976937290416771No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG12990.2963250222414855TruSeq Adapter, Index 27 (100% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG11540.2632479412368547TruSeq Adapter, Index 27 (100% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC10070.22971462463216005No Hit
AGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC9350.21329014302986063TruSeq Adapter, Index 27 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC8920.20348107762848733TruSeq Adapter, Index 27 (100% over 21bp)
AAAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC8910.20325295982845543TruSeq Adapter, Index 27 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8410.1918470698268586No Hit
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG8170.18637224262609212TruSeq Adapter, Index 27 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG7370.16812281862353717TruSeq Adapter, Index 27 (95% over 24bp)
AGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT7250.16538540502315396TruSeq Adapter, Index 27 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG7140.16287610922280266TruSeq Adapter, Index 27 (100% over 23bp)
AGGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC6480.14782033442069487TruSeq Adapter, Index 27 (100% over 21bp)
GACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT6440.1469078632205671TruSeq Adapter, Index 27 (95% over 23bp)
GGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT6430.14667974542053516TruSeq Adapter, Index 27 (100% over 22bp)
ATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC6010.13709879781919385TruSeq Adapter, Index 27 (95% over 22bp)
GGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC5860.1336770308187148TruSeq Adapter, Index 27 (100% over 21bp)
GGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC5710.13025526381823574TruSeq Adapter, Index 27 (100% over 21bp)
ATAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC5700.1300271460182038TruSeq Adapter, Index 27 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT5520.12592102561762894TruSeq Adapter, Index 27 (100% over 22bp)
AGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC5320.12135866961699021TruSeq Adapter, Index 27 (95% over 22bp)
GGTATGGGAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC5240.11953372721673472No Hit
AAGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC4840.11040901521545726TruSeq Adapter, Index 27 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG4780.10904030841526564TruSeq Adapter, Index 27 (100% over 23bp)
GAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT4600.10493418801469079TruSeq Adapter, Index 27 (95% over 23bp)
GAAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC4550.1037935990145311TruSeq Adapter, Index 27 (100% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG4520.1031092456144353TruSeq Adapter, Index 27 (100% over 23bp)
AGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT4390.10014371421402013TruSeq Adapter, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATG302.1624164E-645.0000046
CAACGGA302.1624164E-645.0000043
ACGCATT650.045.00000417
TAGCGGA700.045.0000043
ATAGCAG302.1624164E-645.0000041
TAACGGA302.1624164E-645.0000043
TGGCATA351.209628E-745.0000046
CATAGGA351.209628E-745.0000043
CTACGCA650.045.00000415
ATCCCCC302.1624164E-645.00000410
ACTACGG302.1624164E-645.0000042
TTGCGAG302.1624164E-645.0000041
TCGAGCG302.1624164E-645.0000041
AGTAGAC302.1624164E-645.00000435
AACGTTC207.0285064E-445.013
CGGGTAC207.0285064E-445.06
CGGAAAT207.0285064E-445.06
TCACCGC551.8189894E-1245.024
CAACGTT207.0285064E-445.012
CACGGCT253.8868442E-545.04
Produced by FastQC (version 0.11.5)