##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552483_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	438370
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3814403357894	33.0	31.0	34.0	30.0	34.0
2	32.55942924926432	34.0	31.0	34.0	31.0	34.0
3	32.56621119145927	34.0	31.0	34.0	31.0	34.0
4	36.08013778315122	37.0	35.0	37.0	35.0	37.0
5	36.05405479389557	37.0	35.0	37.0	35.0	37.0
6	36.075185345712526	37.0	35.0	37.0	35.0	37.0
7	36.06870451901362	37.0	36.0	37.0	35.0	37.0
8	36.077379838948836	37.0	35.0	37.0	35.0	37.0
9	37.83414239113078	39.0	38.0	39.0	35.0	39.0
10	37.75832287793416	39.0	37.0	39.0	35.0	39.0
11	37.797164495745605	39.0	37.0	39.0	35.0	39.0
12	37.81393799758195	39.0	38.0	39.0	35.0	39.0
13	37.72063553619089	39.0	37.0	39.0	35.0	39.0
14	38.98054611401328	40.0	38.0	41.0	35.0	41.0
15	38.98562857859799	40.0	38.0	41.0	35.0	41.0
16	38.992875881105	40.0	38.0	41.0	35.0	41.0
17	38.9349841458129	40.0	38.0	41.0	35.0	41.0
18	38.88779797887629	40.0	38.0	41.0	35.0	41.0
19	38.89765038665967	40.0	38.0	41.0	35.0	41.0
20	38.76407144649497	40.0	38.0	41.0	35.0	41.0
21	38.76510253895111	40.0	38.0	41.0	35.0	41.0
22	38.76752971234345	40.0	38.0	41.0	35.0	41.0
23	38.82219586194311	40.0	38.0	41.0	35.0	41.0
24	38.76749549467345	40.0	38.0	41.0	35.0	41.0
25	38.71758560120446	40.0	38.0	41.0	35.0	41.0
26	38.67473367246846	40.0	38.0	41.0	35.0	41.0
27	38.587179779638205	40.0	38.0	41.0	35.0	41.0
28	38.578175970070944	40.0	38.0	41.0	35.0	41.0
29	38.55876770764423	40.0	38.0	41.0	35.0	41.0
30	38.31065538243949	40.0	38.0	41.0	34.0	41.0
31	38.30115427606816	40.0	38.0	41.0	34.0	41.0
32	38.17448502406643	40.0	38.0	41.0	34.0	41.0
33	38.10962657116135	40.0	38.0	41.0	34.0	41.0
34	38.04797089216871	40.0	38.0	41.0	34.0	41.0
35	37.99846248602778	40.0	38.0	41.0	34.0	41.0
36	37.90897415425326	40.0	38.0	41.0	34.0	41.0
37	37.82673768734174	40.0	38.0	41.0	34.0	41.0
38	37.78282729201359	40.0	38.0	41.0	34.0	41.0
39	37.78491456988389	40.0	38.0	41.0	33.0	41.0
40	37.74603417204644	40.0	38.0	41.0	33.0	41.0
41	37.640988206309736	40.0	38.0	41.0	33.0	41.0
42	37.59333667906107	40.0	37.0	41.0	33.0	41.0
43	37.56024819216643	40.0	37.0	41.0	33.0	41.0
44	37.42961881515615	40.0	37.0	41.0	33.0	41.0
45	37.50878709765723	40.0	37.0	41.0	33.0	41.0
46	37.40480187969067	40.0	37.0	41.0	33.0	41.0
47	37.31840454410658	40.0	37.0	41.0	33.0	41.0
48	37.396090060907454	40.0	37.0	41.0	33.0	41.0
49	37.39244473846294	40.0	37.0	41.0	33.0	41.0
50	37.32599174213564	40.0	37.0	41.0	33.0	41.0
51	36.71863494308461	39.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	3.0
12	3.0
13	5.0
14	8.0
15	20.0
16	22.0
17	40.0
18	97.0
19	170.0
20	305.0
21	567.0
22	918.0
23	1736.0
24	2547.0
25	3318.0
26	2938.0
27	2872.0
28	2797.0
29	2925.0
30	3415.0
31	4375.0
32	5935.0
33	8083.0
34	15059.0
35	20847.0
36	22849.0
37	39607.0
38	95119.0
39	201573.0
40	211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.759039167826266	24.8607340830805	23.852681524739374	12.527545224353856
2	31.128270638957957	25.950224696033033	23.07708100463079	19.84442366037822
3	28.95978283185437	28.616237425006275	25.473458493966284	16.95052124917307
4	30.078700641011018	26.622715970527178	23.630038551908207	19.668544836553597
5	25.765449278007164	28.211100212149553	23.327554349065856	22.695896160777426
6	33.123617035837306	26.76757077354746	21.641307571229785	18.46750461938545
7	68.38378538677374	5.731231608002372	15.596185870383467	10.288797134840431
8	57.20327577160846	9.045098889066313	20.12478043661747	13.626844902707758
9	28.256267536555878	26.875470492962567	23.292880443461	21.575381527020554
10	23.13958528183954	26.082761137851584	32.620161051166825	18.15749252914205
11	19.095056687273306	22.763190911786847	33.2235326322513	24.91821976868855
12	21.704952437438692	21.903414923466478	37.22380637361133	19.167826265483495
13	16.943677715172115	31.210621164769485	33.268015603257524	18.577685516800877
14	13.428838652280037	28.124187330337385	37.11248488719575	21.334489130186828
15	13.387093094874192	30.780847229509316	34.81511052307412	21.01694915254237
16	13.29151173666081	27.919109428108673	28.76519834842713	30.024180486803388
17	14.345644090608388	27.684148094075784	28.476629331386725	29.4935784839291
18	15.736706435203137	28.596619294203528	35.62720989118781	20.039464379405526
19	18.076054474530647	25.703857471998543	28.579282341401104	27.64080571206971
20	14.903620229486506	32.15913497730228	32.40253666993635	20.534708123274857
21	15.830462851016264	28.04822410292675	31.581768825421445	24.539544220635538
22	13.395761571275406	30.01391518580195	28.558067385998132	28.032255856924515
23	13.591030408102744	25.680589456395282	30.47448502406643	30.253895111435547
24	20.902434016926343	25.82019755001483	28.364395373771018	24.912973059287815
25	13.34443506626822	30.899924721125988	26.02276615644319	29.7328740561626
26	14.26329356479686	29.92814289298994	25.181011474325345	30.62755206788786
27	18.93035563565025	28.72048725962087	27.780185687889226	24.568971416839656
28	12.767525149987454	27.61845016766658	23.320254579464837	36.293770102881126
29	14.753290599265462	22.674681205374455	25.25264046353537	37.31938773182472
30	16.785592079749982	31.260122727376416	25.377192782352807	26.57709241052079
31	14.029472819764127	25.892054657024886	25.283664484339713	34.794808038871274
32	13.035335447224947	27.10951935579533	26.509569541711343	33.34557565526838
33	13.829641626936152	20.748682619704816	31.56329128361886	33.85838446974017
34	13.142094577639893	18.671441932614	32.99815224581974	35.188311243926364
35	12.769121974587677	19.67607272395465	34.50418596163058	33.05061933982709
36	18.478226155986953	22.765700207587198	27.997125715719594	30.75894792070625
37	13.48449939548783	22.632479412368546	35.97303647603623	27.9099847161074
38	15.030453726304263	20.40057485685608	28.720259141820836	35.84871227501882
39	22.021351826082988	17.12776877979789	33.951228414353174	26.899650979765948
40	17.15149303100121	16.823687752355315	37.045874489586424	28.978944727057055
41	17.400825786436116	22.45249446814335	28.43830554098136	31.70837420443917
42	16.277117503478795	20.170403996623858	31.360950794990533	32.19152770490681
43	21.829504756256128	17.774482742888427	28.410703287177498	31.985309213677944
44	15.853502748819489	18.11437826493601	31.2192896411707	34.8128293450738
45	19.270707393297897	14.837237949677212	32.69703674977759	33.195017907247305
46	20.543376599676073	16.100554326254077	36.71829732874056	26.637771745329285
47	13.810479731733466	17.127540661997855	41.66662864703333	27.395350959235348
48	15.474599082966446	14.57878048224103	33.25181923945525	36.69480119533727
49	18.5685608047996	13.385952505874032	41.23845153637338	26.807035152952984
50	18.806031434632846	12.917398544608435	34.471565116226024	33.8050049045327
51	17.44622122864247	13.164906357643085	32.51522686315213	36.87364555056231
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	930.0
1	837.5
2	745.0
3	875.5
4	1006.0
5	888.5
6	771.0
7	877.0
8	983.0
9	1138.0
10	1293.0
11	1523.5
12	1754.0
13	1817.0
14	1880.0
15	2063.0
16	2246.0
17	2175.5
18	2105.0
19	1970.5
20	1836.0
21	1766.5
22	1697.0
23	1671.5
24	1646.0
25	1824.5
26	2124.0
27	2245.0
28	2412.0
29	2579.0
30	3032.0
31	3485.0
32	3923.5
33	4362.0
34	5032.5
35	5703.0
36	6337.0
37	6971.0
38	8071.5
39	9172.0
40	10751.0
41	12330.0
42	16358.5
43	20387.0
44	27434.5
45	34482.0
46	50238.5
47	65995.0
48	75240.0
49	84485.0
50	78502.5
51	72520.0
52	54737.0
53	36954.0
54	28977.0
55	21000.0
56	16170.5
57	11341.0
58	9321.5
59	7302.0
60	6329.5
61	5357.0
62	4717.0
63	4077.0
64	3243.5
65	2410.0
66	1993.0
67	1576.0
68	1204.5
69	833.0
70	697.0
71	561.0
72	596.5
73	632.0
74	409.5
75	211.0
76	235.0
77	199.0
78	163.0
79	113.5
80	64.0
81	56.5
82	49.0
83	31.0
84	13.0
85	8.0
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	438370.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.364267793398845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.08566230017574	19.7627847959326
2	13.88045712407131	8.429398344201841
3	6.329669270349626	5.765873183056306
4	3.4857980085485796	4.233748168210619
5	2.2779865916096913	3.458469744870428
6	1.473991438399622	2.685400245644597
7	1.0442591489998498	2.2195715112156758
8	0.8224378992491739	1.9978179692993838
9	0.6587501188749197	1.8002218516606252
>10	4.615391493784246	26.35807792573866
>50	0.18605520339271045	3.833183828605914
>100	0.11553427952611858	7.448363442320531
>500	0.013504006697858017	2.829954400830289
>1k	0.008252448537579898	4.815000094208308
>5k	0.002250667782976336	4.362134494204235
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	7297	1.6645755868330405	TruSeq Adapter, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	6198	1.4138741245979425	TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	5654	1.2897780413805688	TruSeq Adapter, Index 27 (100% over 25bp)
TGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT	3688	0.8412984465177819	TruSeq Adapter, Index 27 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	3538	0.8070807765129914	TruSeq Adapter, Index 27 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2871	0.6549262038916897	TruSeq Adapter, Index 27 (100% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	1699	0.3875721422542601	TruSeq Adapter, Index 27 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	1604	0.3659009512512261	No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	1544	0.3522138832493099	TruSeq Adapter, Index 27 (100% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	1428	0.3257522184456053	TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC	1305	0.2976937290416771	No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	1299	0.2963250222414855	TruSeq Adapter, Index 27 (100% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	1154	0.2632479412368547	TruSeq Adapter, Index 27 (100% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	1007	0.22971462463216005	No Hit
AGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	935	0.21329014302986063	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	892	0.20348107762848733	TruSeq Adapter, Index 27 (100% over 21bp)
AAAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	891	0.20325295982845543	TruSeq Adapter, Index 27 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	841	0.1918470698268586	No Hit
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	817	0.18637224262609212	TruSeq Adapter, Index 27 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	737	0.16812281862353717	TruSeq Adapter, Index 27 (95% over 24bp)
AGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	725	0.16538540502315396	TruSeq Adapter, Index 27 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	714	0.16287610922280266	TruSeq Adapter, Index 27 (100% over 23bp)
AGGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	648	0.14782033442069487	TruSeq Adapter, Index 27 (100% over 21bp)
GACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	644	0.1469078632205671	TruSeq Adapter, Index 27 (95% over 23bp)
GGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	643	0.14667974542053516	TruSeq Adapter, Index 27 (100% over 22bp)
ATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	601	0.13709879781919385	TruSeq Adapter, Index 27 (95% over 22bp)
GGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	586	0.1336770308187148	TruSeq Adapter, Index 27 (100% over 21bp)
GGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	571	0.13025526381823574	TruSeq Adapter, Index 27 (100% over 21bp)
ATAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	570	0.1300271460182038	TruSeq Adapter, Index 27 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	552	0.12592102561762894	TruSeq Adapter, Index 27 (100% over 22bp)
AGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	532	0.12135866961699021	TruSeq Adapter, Index 27 (95% over 22bp)
GGTATGGGAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC	524	0.11953372721673472	No Hit
AAGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	484	0.11040901521545726	TruSeq Adapter, Index 27 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	478	0.10904030841526564	TruSeq Adapter, Index 27 (100% over 23bp)
GAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	460	0.10493418801469079	TruSeq Adapter, Index 27 (95% over 23bp)
GAAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	455	0.1037935990145311	TruSeq Adapter, Index 27 (100% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	452	0.1031092456144353	TruSeq Adapter, Index 27 (100% over 23bp)
AGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	439	0.10014371421402013	TruSeq Adapter, Index 27 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.56235600063873E-4	0.0	0.0	1.317836530784497	0.0
2	4.56235600063873E-4	0.0	0.0	5.9771425964368	0.0
3	4.56235600063873E-4	0.0	0.0	8.211328329949586	0.0
4	4.56235600063873E-4	0.0	0.0	10.498893628669846	0.0
5	4.56235600063873E-4	0.0	0.0	17.02329082738326	0.0
6	4.56235600063873E-4	0.0	0.0	19.583685014941715	0.0
7	4.56235600063873E-4	0.0	0.0	23.245888176654425	0.0
8	4.56235600063873E-4	0.0	0.0	26.71578803294021	0.0
9	4.56235600063873E-4	0.0	0.0	28.710678194219494	0.0
10	4.56235600063873E-4	0.0	0.0	31.616898966626366	0.0
11	4.56235600063873E-4	0.0	0.0	36.56158040011862	0.0
12	4.56235600063873E-4	0.0	0.0	39.59828455414376	0.0
13	4.56235600063873E-4	0.0	0.0	40.67659739489472	0.0
14	4.56235600063873E-4	0.0	0.0	41.39471222939526	0.0
15	4.56235600063873E-4	0.0	0.0	42.9456851518124	0.0
16	4.56235600063873E-4	0.0	0.0	45.47893332116705	0.0
17	4.56235600063873E-4	0.0	0.0	50.10174053881424	0.0
18	4.56235600063873E-4	0.0	0.0	51.48709993840819	0.0
19	6.843534000958095E-4	0.0	0.0	53.15235987864133	0.0
20	6.843534000958095E-4	0.0	0.0	54.37415881561238	0.0
21	9.12471200127746E-4	0.0	0.0	55.73100349020234	0.0
22	9.12471200127746E-4	0.0	0.0	57.02625635878368	0.0
23	9.12471200127746E-4	0.0	0.0	57.90610671350686	0.0
24	0.0011405890001596824	0.0	0.0	58.69881606861784	0.0
25	0.0011405890001596824	0.0	0.0	59.417159020918405	0.0
26	0.0011405890001596824	0.0	0.0	60.21922120583069	0.0
27	0.0011405890001596824	0.0	0.0	60.86935693592171	0.0
28	0.0011405890001596824	0.0	0.0	61.6171270844264	0.0
29	0.0011405890001596824	0.0	0.0	62.37607500513265	0.0
30	0.0011405890001596824	0.0	0.0	63.042863334626	0.0
31	0.0011405890001596824	0.0	0.0	63.65011291831102	0.0
32	0.0011405890001596824	0.0	0.0	64.21767000479048	0.0
33	0.0015968246002235555	0.0	0.0	64.8249195884755	0.0
34	0.001824942400255492	0.0	0.0	65.41346351255788	0.0
35	0.001824942400255492	0.0	0.0	65.92695668042977	0.0
36	0.001824942400255492	0.0	0.0	66.49268882450897	0.0
37	0.001824942400255492	0.0	0.0	66.98108903437735	0.0
38	0.001824942400255492	0.0	0.0	67.50667244565093	0.0
39	0.002281178000319365	0.0	0.0	67.97157652211602	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTATG	30	2.1624164E-6	45.000004	6
CAACGGA	30	2.1624164E-6	45.000004	3
ACGCATT	65	0.0	45.000004	17
TAGCGGA	70	0.0	45.000004	3
ATAGCAG	30	2.1624164E-6	45.000004	1
TAACGGA	30	2.1624164E-6	45.000004	3
TGGCATA	35	1.209628E-7	45.000004	6
CATAGGA	35	1.209628E-7	45.000004	3
CTACGCA	65	0.0	45.000004	15
ATCCCCC	30	2.1624164E-6	45.000004	10
ACTACGG	30	2.1624164E-6	45.000004	2
TTGCGAG	30	2.1624164E-6	45.000004	1
TCGAGCG	30	2.1624164E-6	45.000004	1
AGTAGAC	30	2.1624164E-6	45.000004	35
AACGTTC	20	7.0285064E-4	45.0	13
CGGGTAC	20	7.0285064E-4	45.0	6
CGGAAAT	20	7.0285064E-4	45.0	6
TCACCGC	55	1.8189894E-12	45.0	24
CAACGTT	20	7.0285064E-4	45.0	12
CACGGCT	25	3.8868442E-5	45.0	4
>>END_MODULE