##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552482_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	475956
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77509475665818	34.0	31.0	34.0	31.0	34.0
2	32.943223743371234	34.0	33.0	34.0	31.0	34.0
3	32.94178663573944	34.0	33.0	34.0	31.0	34.0
4	36.31846851389624	37.0	37.0	37.0	35.0	37.0
5	36.306024086260074	37.0	37.0	37.0	35.0	37.0
6	36.33087512291052	37.0	37.0	37.0	35.0	37.0
7	36.26204523107178	37.0	37.0	37.0	35.0	37.0
8	36.30006345124339	37.0	37.0	37.0	35.0	37.0
9	38.063638235467145	39.0	38.0	39.0	37.0	39.0
10	37.98019985040634	39.0	38.0	39.0	35.0	39.0
11	38.00860373647984	39.0	38.0	39.0	35.0	39.0
12	38.02544142735883	39.0	38.0	39.0	35.0	39.0
13	37.95530469203035	39.0	38.0	39.0	35.0	39.0
14	39.37309121011186	41.0	39.0	41.0	36.0	41.0
15	39.40660691324408	41.0	39.0	41.0	36.0	41.0
16	39.38713032297103	41.0	39.0	41.0	36.0	41.0
17	39.34574624545126	41.0	39.0	41.0	36.0	41.0
18	39.30380749481044	41.0	39.0	41.0	36.0	41.0
19	39.30403440654178	41.0	39.0	41.0	35.0	41.0
20	39.2290884031297	41.0	39.0	41.0	35.0	41.0
21	39.16836220154804	41.0	39.0	41.0	35.0	41.0
22	39.16364537898461	40.0	39.0	41.0	35.0	41.0
23	39.1761969593828	41.0	39.0	41.0	35.0	41.0
24	39.1547411945642	41.0	39.0	41.0	35.0	41.0
25	39.08965324525796	41.0	39.0	41.0	35.0	41.0
26	39.03675339737287	40.0	39.0	41.0	35.0	41.0
27	38.97879425829279	40.0	39.0	41.0	35.0	41.0
28	38.93282362235165	40.0	39.0	41.0	35.0	41.0
29	38.90633587978721	40.0	38.0	41.0	35.0	41.0
30	38.754655892561495	40.0	38.0	41.0	35.0	41.0
31	38.708985704560924	40.0	38.0	41.0	35.0	41.0
32	38.59515165267378	40.0	38.0	41.0	35.0	41.0
33	38.503542344250306	40.0	38.0	41.0	35.0	41.0
34	38.4097458588609	40.0	38.0	41.0	35.0	41.0
35	38.293243493096	40.0	38.0	41.0	35.0	41.0
36	38.247943087176125	40.0	38.0	41.0	35.0	41.0
37	38.17668439939826	40.0	38.0	41.0	35.0	41.0
38	38.072897494726405	40.0	38.0	41.0	34.0	41.0
39	38.016881812604524	40.0	38.0	41.0	34.0	41.0
40	37.96535183924564	40.0	37.0	41.0	34.0	41.0
41	37.91326088966207	40.0	37.0	41.0	34.0	41.0
42	37.84946717763827	40.0	37.0	41.0	34.0	41.0
43	37.85575767507921	40.0	37.0	41.0	34.0	41.0
44	37.74221356595988	40.0	37.0	41.0	34.0	41.0
45	37.73680760406424	40.0	37.0	41.0	34.0	41.0
46	37.62854339476758	40.0	37.0	41.0	34.0	41.0
47	37.54549160006387	40.0	36.0	41.0	34.0	41.0
48	37.51852692265672	40.0	36.0	41.0	34.0	41.0
49	37.46775962483927	40.0	36.0	41.0	33.0	41.0
50	37.402955315197204	40.0	36.0	41.0	33.0	41.0
51	36.807490608375566	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	5.0
12	8.0
13	5.0
14	20.0
15	15.0
16	25.0
17	46.0
18	84.0
19	97.0
20	221.0
21	419.0
22	690.0
23	1068.0
24	1814.0
25	2236.0
26	2621.0
27	2817.0
28	3003.0
29	3143.0
30	3478.0
31	4017.0
32	5093.0
33	7024.0
34	15729.0
35	22767.0
36	23042.0
37	38883.0
38	86472.0
39	250380.0
40	730.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.95525636823572	23.27315970383817	22.811772516787265	12.959811411138844
2	33.82707645244518	25.29435494037264	22.627721890258766	18.250846716923412
3	33.141508878972004	26.050727378161003	23.451957743993145	17.355805998873848
4	30.551983796821553	28.47637176545731	21.015808183949776	19.955836253771356
5	29.51008076376808	31.925430081772266	18.578187899721822	19.986301254737832
6	39.21433914059283	28.338964105925758	16.125230063283162	16.321466690198253
7	78.66756590945381	5.255527821899504	9.721696963584868	6.355209305061813
8	70.4067182680752	8.705846758944103	12.466278395481934	8.42115657749876
9	35.789862928506	27.332358453302408	18.450234895662625	18.427543722528974
10	26.82180705779526	26.583339636436982	27.563262150282796	19.03159115548496
11	24.69030750741665	24.661523334089704	30.198589785610437	20.449579372883207
12	24.121557454890787	23.382623603862545	31.89139332207179	20.604425619174883
13	21.63183151383741	27.65549756700199	30.850961013202898	19.861709905957696
14	19.702031280202373	27.286766003580166	32.79420786795418	20.216994848263283
15	22.564060543411575	26.66107791476523	31.1726294027179	19.602232139105297
16	21.721965896007195	26.757935607493128	28.06204775231324	23.458050744186437
17	21.757473379892257	27.42963635294019	28.06561951104724	22.747270756120315
18	22.02094311238854	27.843540159174378	30.075679264469823	20.05983746396726
19	25.104841624015666	25.467480187244202	27.6744068779467	21.753271310793433
20	23.298792325340997	27.706552706552706	29.096176957533892	19.898478010572408
21	22.33945995007942	27.147047206044256	28.96654312583516	21.546949718041162
22	20.8981502491827	27.36177293699418	28.073183235425127	23.666893578398003
23	20.9017220079167	25.59018060492987	30.134088024943484	23.374009362209954
24	22.193438048895274	26.62094815487146	28.290640311289277	22.894973484943986
25	20.342846817773072	29.17643647732143	26.823277781979847	23.65743892292565
26	19.867592802696045	28.375942313995413	29.218457168309676	22.538007714998866
27	21.476144853725977	26.498247737185793	30.953491499214213	21.07211590987402
28	19.873685802889344	26.708771399036884	29.363008345309233	24.054534452764543
29	20.53656220322887	25.2222894553278	29.585507904091973	24.65564043735135
30	21.913790350368522	26.38374135424283	28.443385523031537	23.25908277235711
31	23.178613149114625	26.802477539940668	28.143988099740312	21.8749212112044
32	20.899410869912344	28.932296262679742	27.41829076637336	22.75000210103455
33	20.575641445847936	26.544680600727798	28.63584028775769	24.24383766566657
34	21.339367504559245	25.46075687668608	29.180008236055432	24.019867382699243
35	20.51198009900075	28.173402583432082	28.151972031028077	23.162645286539092
36	22.51132457622133	28.535831043205672	27.86938288413215	21.083461496440847
37	21.497995613039862	27.13906327475649	29.406709863937003	21.956231248266647
38	20.93134659506341	28.297153518392452	26.379329181689066	24.392170704855072
39	23.017884006084596	25.88894771785627	27.86707174612779	23.22609652993134
40	20.808856280832682	25.594382674028694	29.534662867996204	24.062098177142424
41	21.546949718041162	27.180243551924967	27.568094529746446	23.70471220028742
42	19.839438939733924	26.735874744724303	30.394196102160702	23.030490213381068
43	21.540646614392926	24.393641429039658	29.867466740623083	24.19824521594433
44	20.11068250006303	24.836749615510676	28.98524233332493	26.067325551101362
45	21.739194379312373	24.292161460303053	27.8895528158065	26.07909134457807
46	22.45732798830144	25.11156493457378	29.52814966089302	22.902957416231754
47	20.093664120212793	25.092655623629074	32.03678491289111	22.776895343267025
48	19.742791350460966	24.55100891679063	29.991007572128513	25.71519216061989
49	21.144601601828743	23.51015640101186	31.461311549807125	23.883930447352274
50	20.583205170225817	24.054534452764543	29.43045155434536	25.93180882266428
51	20.200396675322928	23.112850767718022	29.647698526754574	27.039054030204472
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	319.0
1	671.0
2	1023.0
3	1492.5
4	1962.0
5	1501.0
6	1040.0
7	1111.5
8	1183.0
9	1267.5
10	1352.0
11	1448.0
12	1544.0
13	1598.5
14	1653.0
15	1634.0
16	1615.0
17	1593.5
18	1572.0
19	1711.0
20	1850.0
21	1935.5
22	2021.0
23	2334.0
24	2647.0
25	3222.5
26	4831.0
27	5864.0
28	6477.5
29	7091.0
30	8326.5
31	9562.0
32	10215.5
33	10869.0
34	11922.0
35	12975.0
36	14162.0
37	15349.0
38	16333.0
39	17317.0
40	19996.0
41	22675.0
42	25998.5
43	29322.0
44	34815.5
45	40309.0
46	45432.0
47	50555.0
48	49337.5
49	48120.0
50	41333.0
51	34546.0
52	31152.0
53	27758.0
54	25849.0
55	23940.0
56	22038.5
57	20137.0
58	18995.0
59	17853.0
60	17057.0
61	16261.0
62	14641.5
63	13022.0
64	11160.0
65	9298.0
66	7613.5
67	5929.0
68	5345.5
69	4762.0
70	4041.0
71	3320.0
72	2770.0
73	2220.0
74	1862.5
75	1217.0
76	929.0
77	717.0
78	505.0
79	348.0
80	191.0
81	151.5
82	112.0
83	79.5
84	47.0
85	38.5
86	30.0
87	16.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	475956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.874381870929195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.44707345525067	21.75009847546809
2	10.971057376484051	6.774492299388865
3	4.008021118670989	3.712355236937908
4	2.054522688166085	2.5372847214771075
5	1.3604753798641136	2.1001918201961054
6	1.049322386208717	1.943830803451355
7	0.8478790805184048	1.8324419758608288
8	0.7396358091559481	1.8268638733835563
9	0.6887631805838377	1.9138623710383056
>10	7.62056786654414	46.597436959947444
>50	0.14655947624647228	2.7938831959860777
>100	0.059987134463672376	3.779897717507341
>500	0.003408359912708658	0.8336400255593864
>1k	0.002726687930166926	1.6037205237976582
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2599	0.5460588793922128	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	1889	0.39688542638395147	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1867	0.3922631503752448	No Hit
TGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT	1265	0.26578087050063454	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	999	0.20989335148627183	No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	869	0.18257990234391414	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCC	822	0.17270503996167713	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	762	0.16009883266520436	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	509	0.10694265856507745	No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG	499	0.10484162401566532	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1010345494121304E-4	0.0	0.0	0.4038188403970115	0.0
2	2.1010345494121304E-4	0.0	0.0	1.7539436418492467	0.0
3	2.1010345494121304E-4	0.0	0.0	2.3903470068661807	0.0
4	2.1010345494121304E-4	0.0	0.0	3.1263394095252504	0.0
5	2.1010345494121304E-4	0.0	0.0	5.108035196530771	0.0
6	2.1010345494121304E-4	0.0	0.0	5.920295153333502	0.0
7	2.1010345494121304E-4	0.0	0.0	7.137004260898066	0.0
8	2.1010345494121304E-4	0.0	0.0	8.477884510332888	0.0
9	2.1010345494121304E-4	0.0	0.0	9.264091638722908	0.0
10	2.1010345494121304E-4	0.0	0.0	10.38520367428922	0.0
11	2.1010345494121304E-4	0.0	0.0	12.184109455495886	0.0
12	2.1010345494121304E-4	0.0	0.0	13.37476573464774	0.0
13	2.1010345494121304E-4	0.0	0.0	13.84056509425241	0.0
14	2.1010345494121304E-4	0.0	0.0	14.136180655354696	0.0
15	2.1010345494121304E-4	0.0	0.0	14.809562228441285	0.0
16	2.1010345494121304E-4	0.0	0.0	15.967022161712427	0.0
17	2.1010345494121304E-4	0.0	0.0	17.53481414248376	0.0
18	2.1010345494121304E-4	0.0	0.0	18.290346166452363	0.0
19	2.1010345494121304E-4	0.0	0.0	19.040625604047435	0.0
20	2.1010345494121304E-4	0.0	0.0	19.682281555437896	0.0
21	2.1010345494121304E-4	0.0	0.0	20.445797510694266	0.0
22	2.1010345494121304E-4	0.0	0.0	21.172755464790864	0.0
23	4.202069098824261E-4	0.0	0.0	21.729319516930136	0.0
24	6.303103648236392E-4	0.0	0.0	22.217389842758575	0.0
25	8.404138197648522E-4	0.0	0.0	22.6962156165696	0.0
26	8.404138197648522E-4	0.0	0.0	23.173360562741095	0.0
27	8.404138197648522E-4	0.0	0.0	23.61268688702317	0.0
28	8.404138197648522E-4	0.0	0.0	24.100126902486785	0.0
29	8.404138197648522E-4	0.0	0.0	24.598492297607343	0.0
30	8.404138197648522E-4	0.0	0.0	25.113455865668254	0.0
31	8.404138197648522E-4	0.0	0.0	25.56980056980057	0.0
32	8.404138197648522E-4	0.0	0.0	26.020682584104414	0.0
33	0.0010505172747060653	0.0	0.0	26.462950356755666	0.0
34	0.0010505172747060653	0.0	0.0	26.96257637260587	0.0
35	0.0010505172747060653	0.0	0.0	27.444343594786073	0.0
36	0.0010505172747060653	0.0	0.0	27.896906436729445	0.0
37	0.0010505172747060653	0.0	0.0	28.36396641706376	0.0
38	0.0010505172747060653	0.0	0.0	28.839640639050668	0.0
39	0.0010505172747060653	0.0	0.0	29.31405424030793	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAA	20	7.029118E-4	45.0	15
CACGGAT	20	7.029118E-4	45.0	4
GCGGTCA	20	7.029118E-4	45.0	5
ACCTGCG	25	3.88735E-5	45.0	11
GTGGCGT	40	6.8012014E-9	45.0	34
CGTTCGC	25	3.88735E-5	45.0	45
TCTAGCG	40	6.8012014E-9	45.0	1
TATTGCG	20	7.029118E-4	45.0	1
GCCGATA	20	7.029118E-4	45.0	8
GTATGCG	45	3.8380676E-10	45.0	1
ATGCGGT	25	3.88735E-5	45.0	3
CGTCATG	20	7.029118E-4	45.0	36
TGTAACG	25	3.88735E-5	45.0	1
CAATAGC	25	3.88735E-5	45.0	34
CGAATAT	85	0.0	42.352943	14
TACGAAA	70	0.0	41.785713	19
ATACGAA	70	0.0	41.785713	18
GGCGATA	165	0.0	40.909092	7
ACGGAGT	50	1.0786607E-9	40.5	5
TTGCGAG	50	1.0786607E-9	40.5	1
>>END_MODULE