FastQCFastQC Report
Sat 18 Jun 2016
SRR3552479_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552479_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553314
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC46330.8373184123300693TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT33100.5982136725259075TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC32770.5922496087212686TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC18930.3421203873388347TruSeq Adapter, Index 20 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTT17940.32422819592491786TruSeq Adapter, Index 20 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC14500.26205734899171174TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCC14430.2607922445483035No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC9480.17133128747871912No Hit
GCCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG8410.15199326241519281No Hit
AGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG8110.1465713862291574No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8090.14620992781675504No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC7780.14060732242451845No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG7450.1346432586198795No Hit
ACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG7390.1335588833826724No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG7050.12741409037183227No Hit
GGAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC6490.11729325482456615No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC5920.10699169007109886No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACT253.888173E-545.0000046
GACGTAG302.1634532E-645.0000041
TAACGGT253.888173E-545.0000043
TCGGATG302.1634532E-645.0000045
GCGATTT302.1634532E-645.0000048
GCGCGAC207.0301123E-445.08
TACGGCA207.0301123E-445.04
CTACGAA453.8380676E-1045.011
GTATACG207.0301123E-445.01
TATAGCG207.0301123E-445.01
GCGGAAC351.2103919E-745.05
TAGTACG207.0301123E-445.01
ACGAAAC207.0301123E-445.020
TTAGCGG750.042.02
AAGCGGT501.0786607E-940.5000043
ACGGACA501.0786607E-940.5000045
TTAGTCG501.0786607E-940.50000412
GGCGATT1900.040.2631537
ACCGCTA451.9252184E-840.026
ATAGACG451.9252184E-840.01
Produced by FastQC (version 0.11.5)