##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552479_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553314
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65628919564659	34.0	31.0	34.0	31.0	34.0
2	32.81703698081017	34.0	31.0	34.0	31.0	34.0
3	32.822796820611806	34.0	33.0	34.0	31.0	34.0
4	36.24982198173189	37.0	37.0	37.0	35.0	37.0
5	36.22633983597017	37.0	37.0	37.0	35.0	37.0
6	36.25206121659672	37.0	37.0	37.0	35.0	37.0
7	36.24554592871317	37.0	37.0	37.0	35.0	37.0
8	36.25414321705216	37.0	37.0	37.0	35.0	37.0
9	37.97437079126861	39.0	38.0	39.0	35.0	39.0
10	37.90438159887514	39.0	38.0	39.0	35.0	39.0
11	37.9292463230643	39.0	38.0	39.0	35.0	39.0
12	37.94937232746686	39.0	38.0	39.0	35.0	39.0
13	37.89843922257525	39.0	38.0	39.0	35.0	39.0
14	39.257407186516154	40.0	39.0	41.0	36.0	41.0
15	39.287278109717086	40.0	39.0	41.0	36.0	41.0
16	39.23137314436288	40.0	39.0	41.0	35.0	41.0
17	39.179162283983416	40.0	39.0	41.0	35.0	41.0
18	39.15921700878705	40.0	39.0	41.0	35.0	41.0
19	39.158750727435056	40.0	39.0	41.0	35.0	41.0
20	39.074330307926424	40.0	39.0	41.0	35.0	41.0
21	39.02456832829099	40.0	39.0	41.0	35.0	41.0
22	39.030825173409674	40.0	39.0	41.0	35.0	41.0
23	39.02635935472444	40.0	39.0	41.0	35.0	41.0
24	38.998601155944	40.0	38.0	41.0	35.0	41.0
25	38.92238403510484	40.0	38.0	41.0	35.0	41.0
26	38.88284409937215	40.0	38.0	41.0	35.0	41.0
27	38.80891139569937	40.0	38.0	41.0	35.0	41.0
28	38.737349859211946	40.0	38.0	41.0	35.0	41.0
29	38.681336456334016	40.0	38.0	41.0	35.0	41.0
30	38.47649616673354	40.0	38.0	41.0	35.0	41.0
31	38.48235721489064	40.0	38.0	41.0	35.0	41.0
32	38.334612896113235	40.0	38.0	41.0	35.0	41.0
33	38.22390541356264	40.0	38.0	41.0	35.0	41.0
34	38.16628171345746	40.0	38.0	41.0	34.0	41.0
35	38.08909226948894	40.0	38.0	41.0	34.0	41.0
36	38.03260897067488	40.0	38.0	41.0	34.0	41.0
37	37.97062427482406	40.0	38.0	41.0	34.0	41.0
38	37.895742742818726	40.0	37.0	41.0	34.0	41.0
39	37.83345080731736	40.0	37.0	41.0	34.0	41.0
40	37.8304850410436	40.0	37.0	41.0	34.0	41.0
41	37.79811282562885	40.0	37.0	41.0	34.0	41.0
42	37.73483953053781	40.0	37.0	41.0	34.0	41.0
43	37.67032101121605	40.0	37.0	41.0	33.0	41.0
44	37.54839747412861	40.0	37.0	41.0	33.0	41.0
45	37.537636857191394	40.0	37.0	41.0	33.0	41.0
46	37.46898867550794	40.0	36.0	41.0	33.0	41.0
47	37.3789783016515	40.0	36.0	41.0	33.0	41.0
48	37.38020183837749	40.0	36.0	41.0	33.0	41.0
49	37.334197218938975	40.0	36.0	41.0	33.0	41.0
50	37.28256649931142	40.0	36.0	41.0	33.0	41.0
51	36.663623186834236	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	5.0
12	7.0
13	10.0
14	7.0
15	18.0
16	45.0
17	80.0
18	143.0
19	256.0
20	313.0
21	520.0
22	831.0
23	1344.0
24	2106.0
25	2907.0
26	3259.0
27	3441.0
28	3682.0
29	3831.0
30	4218.0
31	5319.0
32	6837.0
33	9311.0
34	19150.0
35	27494.0
36	29047.0
37	49241.0
38	111220.0
39	268082.0
40	583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73977163057504	22.810013843857195	22.591331504353768	12.858883021213993
2	32.574270667288374	24.86363981392121	22.70808257155973	19.854006947230687
3	33.99281420676144	25.72101916813961	23.339911876439057	16.946254748659893
4	31.934489277336198	27.168479380604865	21.38821717867251	19.508814163386432
5	28.849803185894444	30.456485829022938	19.613456373776916	21.080254611305698
6	37.660351988201995	28.477501021120016	17.603024683994985	16.259122306683004
7	77.10504342922825	4.883122422349697	11.127497225806685	6.884336922615368
8	67.6841720975793	8.077872600367964	14.693284464155976	9.544670837896746
9	33.684128722569824	26.287605229580315	20.548549286661824	19.479716761188044
10	26.90606057320075	25.824034815674285	28.798114632920907	18.471789978204058
11	24.01547764921907	24.580798606216362	29.859537260940446	21.544186483624124
12	22.936705017404222	22.86911229428498	33.1589296493492	21.035253038961603
13	21.07031450496463	27.24257835514735	32.75734935317017	18.92975778671785
14	19.43236570916333	26.891421507498453	32.90102907210011	20.775183711238103
15	20.85434310355422	26.81388867803815	32.0882898318134	20.24347838659423
16	20.54981439110523	26.1793484350658	28.941071435026046	24.329765738802923
17	20.526681052711478	27.048836646099684	28.58449271119111	23.839989589997725
18	21.093809301770786	27.583975825661376	31.31404591244754	20.008168960120294
19	22.9021857390198	25.266304485337436	28.910347469971843	22.921162305670922
20	20.66403524942438	28.7513057685148	30.42124363381371	20.163415348247106
21	20.79144933979621	26.750994914280135	31.100785449130154	21.356770296793503
22	19.54658656748248	26.95214652078205	28.588468753727543	24.91279815800793
23	19.019218743787434	25.89524212291755	30.099907105188013	24.985632028107005
24	22.18776318690653	25.61872643742974	28.94866206168649	23.244848313977233
25	18.05990811727157	27.985736851046607	28.795403694827893	25.158951336853942
26	20.170463787288952	26.65810010229273	29.112944910123367	24.058491200294952
27	21.52033022840557	26.24585678294784	30.455762912198136	21.778050076448455
28	18.5545639546442	26.831419411039665	27.617772187221	26.99624444709514
29	21.53261981442725	25.999161416483226	27.973989452643526	24.494229316445995
30	21.48816042970176	28.78437921324962	27.6794008465356	22.048059510513017
31	21.01157751294925	26.986665799166477	26.61544800962925	25.38630867825502
32	19.913286126864673	28.804982342756553	26.13904582208294	25.14268570829583
33	18.810837969037472	25.994281727915798	28.021701963080638	27.173178339966096
34	18.605529590792933	25.32323418529081	29.99616854082853	26.07506768308772
35	20.205705982498184	26.84949233165978	26.520745905579833	26.4240557802622
36	21.68696255652306	25.98578745522434	27.25306064910702	25.074189339145587
37	18.147019594660534	26.278388040064048	30.314794131361218	25.2597982339142
38	19.325735477504637	27.505900808582467	24.838156995846845	28.33020671806605
39	21.769194345344598	26.18802343696346	28.131404591244753	23.911377626447187
40	20.152933054287438	23.420517102404784	31.048373979331807	25.378175863975972
41	20.706325883675454	23.851014071575996	26.882204317982193	28.56045572676636
42	19.062232294863314	22.048782427337823	29.714411708360895	29.174573569437968
43	21.840220923381658	23.71890102184293	28.111343649356424	26.329534405418986
44	18.996808322218488	25.41667118489682	28.772631814846545	26.81388867803815
45	20.658974831650745	23.590583285440093	28.305085358404085	27.445356524505073
46	21.376289051063228	22.700672674105483	30.84686091441749	25.076177360413798
47	17.60862728938722	22.417650737194432	33.100373386540014	26.873348586878336
48	18.66065199868429	21.900403749046653	29.830259129535854	29.608685122733203
49	19.989553851881574	20.994588967566337	32.91946345113263	26.09639372941946
50	19.67400065785431	20.45131697372559	30.998131260007877	28.876551108412222
51	19.23428649916684	19.57767199094908	29.730858066125204	31.45718344375888
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	382.0
1	742.0
2	1102.0
3	1594.5
4	2087.0
5	1683.5
6	1280.0
7	1364.0
8	1448.0
9	1616.5
10	1785.0
11	1854.0
12	1923.0
13	2005.0
14	2087.0
15	2047.0
16	2007.0
17	2095.0
18	2183.0
19	2071.0
20	1959.0
21	2128.0
22	2297.0
23	2436.5
24	2576.0
25	2917.5
26	4401.0
27	5543.0
28	6102.5
29	6662.0
30	7430.5
31	8199.0
32	9007.5
33	9816.0
34	10735.0
35	11654.0
36	12695.5
37	13737.0
38	15110.0
39	16483.0
40	18591.5
41	20700.0
42	23854.0
43	27008.0
44	31153.5
45	35299.0
46	45260.0
47	55221.0
48	64724.0
49	74227.0
50	73451.0
51	72675.0
52	59690.0
53	46705.0
54	39448.5
55	32192.0
56	27313.0
57	22434.0
58	20073.5
59	17713.0
60	16189.0
61	14665.0
62	12829.0
63	10993.0
64	9664.5
65	8336.0
66	6802.0
67	5268.0
68	4425.0
69	3582.0
70	3057.0
71	2532.0
72	2177.5
73	1823.0
74	1649.5
75	1192.0
76	908.0
77	724.0
78	540.0
79	441.0
80	342.0
81	231.5
82	121.0
83	80.5
84	40.0
85	35.5
86	31.0
87	19.0
88	7.0
89	6.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	553314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.396955200319056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.68744202935339	23.28241911107862
2	13.347913299558368	9.18255151565304
3	5.262105241949406	5.430011948000934
4	2.638511488636368	3.6302704588060917
5	1.7000171158768205	2.9237706287295295
6	1.1726875355365758	2.4202128374294496
7	0.9009508941226161	2.1692977279976113
8	0.760810605923653	2.093565466230679
9	0.6656000200750665	2.060515266466817
>10	5.681263689898537	35.2740517945162
>50	0.11267259054991403	2.5285534634766984
>100	0.05949533986976184	4.112113947977463
>500	0.006844596622184991	1.6690408766211706
>1k	0.003685552027330379	3.223624957015715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	4633	0.8373184123300693	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	3310	0.5982136725259075	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	3277	0.5922496087212686	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	1893	0.3421203873388347	TruSeq Adapter, Index 20 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTT	1794	0.32422819592491786	TruSeq Adapter, Index 20 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	1450	0.26205734899171174	TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCC	1443	0.2607922445483035	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	948	0.17133128747871912	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	841	0.15199326241519281	No Hit
AGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	811	0.1465713862291574	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	809	0.14620992781675504	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC	778	0.14060732242451845	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG	745	0.1346432586198795	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	739	0.1335588833826724	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	705	0.12741409037183227	No Hit
GGAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	649	0.11729325482456615	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	592	0.10699169007109886	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8072920620118053E-4	0.0	0.0	0.6114069045785937	0.0
2	1.8072920620118053E-4	0.0	0.0	2.6903349635107734	0.0
3	1.8072920620118053E-4	0.0	0.0	3.6756705957196094	0.0
4	1.8072920620118053E-4	0.0	0.0	4.764925521494124	0.0
5	1.8072920620118053E-4	0.0	0.0	7.85828661483353	0.0
6	1.8072920620118053E-4	0.0	0.0	9.028146766573771	0.0
7	1.8072920620118053E-4	0.0	0.0	10.827486743512726	0.0
8	1.8072920620118053E-4	0.0	0.0	12.869546044379863	0.0
9	1.8072920620118053E-4	0.0	0.0	14.087299435763418	0.0
10	1.8072920620118053E-4	0.0	0.0	15.871638888587674	0.0
11	1.8072920620118053E-4	0.0	0.0	18.589444691441027	0.0
12	1.8072920620118053E-4	0.0	0.0	20.437220095641894	0.0
13	1.8072920620118053E-4	0.0	0.0	21.141341083001695	0.0
14	1.8072920620118053E-4	0.0	0.0	21.6012969127837	0.0
15	1.8072920620118053E-4	0.0	0.0	22.656032560173788	0.0
16	1.8072920620118053E-4	0.0	0.0	24.445432430771678	0.0
17	1.8072920620118053E-4	0.0	0.0	26.78840585996378	0.0
18	1.8072920620118053E-4	0.0	0.0	27.960434762178437	0.0
19	1.8072920620118053E-4	0.0	0.0	29.17204336055115	0.0
20	1.8072920620118053E-4	0.0	0.0	30.22334515302342	0.0
21	9.036460310059026E-4	0.0	0.0	31.42067614410624	0.0
22	9.036460310059026E-4	0.0	0.0	32.49547273338466	0.0
23	9.036460310059026E-4	0.0	0.0	33.429662000238565	0.0
24	0.0010843752372070832	0.0	0.0	34.19739966818118	0.0
25	0.0010843752372070832	0.0	0.0	34.92826857805875	0.0
26	0.0010843752372070832	0.0	0.0	35.70811510281685	0.0
27	0.0010843752372070832	0.0	0.0	36.40446473431	0.0
28	0.0010843752372070832	0.0	0.0	37.131719060063546	0.0
29	0.0010843752372070832	0.0	0.0	37.88409474547906	0.0
30	0.0010843752372070832	0.0	0.0	38.5949027134683	0.0
31	0.0012651044434082636	0.0	0.0	39.30480703542654	0.0
32	0.0012651044434082636	0.0	0.0	39.987963434867	0.0
33	0.0016265628558106246	0.0	0.0	40.687024004453164	0.0
34	0.0016265628558106246	0.0	0.0	41.37180696674944	0.0
35	0.0016265628558106246	0.0	0.0	42.013576377969834	0.0
36	0.0016265628558106246	0.0	0.0	42.66998485489252	0.0
37	0.0016265628558106246	0.0	0.0	43.36217771464304	0.0
38	0.0019880212682129856	0.0	0.0	44.01948983759674	0.0
39	0.0019880212682129856	0.0	0.0	44.74114155795805	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACT	25	3.888173E-5	45.000004	6
GACGTAG	30	2.1634532E-6	45.000004	1
TAACGGT	25	3.888173E-5	45.000004	3
TCGGATG	30	2.1634532E-6	45.000004	5
GCGATTT	30	2.1634532E-6	45.000004	8
GCGCGAC	20	7.0301123E-4	45.0	8
TACGGCA	20	7.0301123E-4	45.0	4
CTACGAA	45	3.8380676E-10	45.0	11
GTATACG	20	7.0301123E-4	45.0	1
TATAGCG	20	7.0301123E-4	45.0	1
GCGGAAC	35	1.2103919E-7	45.0	5
TAGTACG	20	7.0301123E-4	45.0	1
ACGAAAC	20	7.0301123E-4	45.0	20
TTAGCGG	75	0.0	42.0	2
AAGCGGT	50	1.0786607E-9	40.500004	3
ACGGACA	50	1.0786607E-9	40.500004	5
TTAGTCG	50	1.0786607E-9	40.500004	12
GGCGATT	190	0.0	40.263153	7
ACCGCTA	45	1.9252184E-8	40.0	26
ATAGACG	45	1.9252184E-8	40.0	1
>>END_MODULE