SRR3552470_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495803
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	4425	0.8924915742744599	Illumina Paired End PCR Primer 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	3175	0.640375310355121	TruSeq Adapter, Index 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	2869	0.5786572489476667	Illumina Paired End PCR Primer 2 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	1908	0.38483026524647895	Illumina Paired End PCR Primer 2 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	1403	0.28297529462306603	TruSeq Adapter, Index 1 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	852	0.17184244548742142	Illumina Paired End PCR Primer 2 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	800	0.1613544089083769	TruSeq Adapter, Index 1 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	698	0.14078172177255888	TruSeq Adapter, Index 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	672	0.13553770348303662	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCC	662	0.1335207733716819	No Hit
TAGGAAGGGCCAAGTGATCCAGTTCTAAAACTTGTGTTCTCTGAGAGGAAA	617	0.12444458787058571	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	572	0.11536840236948949	TruSeq Adapter, Index 1 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	537	0.108309146979748	Illumina Paired End PCR Primer 2 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGC	35	1.2100645E-7	45.000004	24
TTGGACG	35	1.2100645E-7	45.000004	1
AATGACG	35	1.2100645E-7	45.000004	10
AGCACGG	35	1.2100645E-7	45.000004	2
CTTCGAA	20	7.0294016E-4	45.0	26
CGGAAAT	20	7.0294016E-4	45.0	23
GGTACTA	20	7.0294016E-4	45.0	8
CGAACCA	20	7.0294016E-4	45.0	15
GCGACTC	20	7.0294016E-4	45.0	13
CGTATGG	50	2.1827873E-11	45.0	2
GACGTGT	25	3.8875853E-5	45.0	9
TAGCGAG	45	3.8380676E-10	45.0	1
ATAGCGA	20	7.0294016E-4	45.0	10
CGCGCCA	20	7.0294016E-4	45.0	13
CGCGAGG	55	1.8189894E-12	45.0	2
TCGGCGA	20	7.0294016E-4	45.0	14
TATTGCG	40	6.8012014E-9	45.0	1
GTTACCG	20	7.0294016E-4	45.0	38
AGGTTAC	40	6.8012014E-9	45.0	6
TCTAACG	20	7.0294016E-4	45.0	1