FastQCFastQC Report
Sat 18 Jun 2016
SRR3552467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995635
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC67440.22512756060067396Illumina PCR Primer Index 9 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT58940.19675294219756412RNA PCR Primer, Index 17 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55550.18543647674032385No Hit
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC43950.1467134680960798RNA PCR Primer, Index 17 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC31700.10582063569159793Illumina PCR Primer Index 9 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA207.0351025E-445.00000425
GCGTAAG3200.041.484381
CGAAAGG7500.039.02
CGTTTTT47150.038.3191951
CGACCAA5250.037.71428329
CGAATAT3050.037.6229514
ACGATAG1200.037.5000041
TCGAAAG3150.037.1428571
AACACGT5200.036.77884741
TCTAGCG1300.036.3461571
GACCGAT13050.036.034489
ACTATCG250.00210764836.017
CGTAAGG5750.036.02
CGCGTAG250.00210764836.01
ATTAGCG1200.035.6250041
ACACGAC5700.035.52631826
TTACGAG950.035.5263141
ACGACCA5600.035.35714328
TACGGGA13500.035.3333324
TATAGCG1850.035.270271
Produced by FastQC (version 0.11.5)