##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552467_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2995635
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66270390084239	31.0	31.0	34.0	30.0	34.0
2	31.827998070525947	31.0	31.0	34.0	30.0	34.0
3	31.85412308241825	33.0	31.0	34.0	30.0	34.0
4	35.49098504991429	37.0	35.0	37.0	33.0	37.0
5	35.47711486880077	37.0	35.0	37.0	33.0	37.0
6	35.49544453847014	37.0	35.0	37.0	33.0	37.0
7	35.91416844842579	37.0	35.0	37.0	35.0	37.0
8	35.95583807773644	37.0	35.0	37.0	35.0	37.0
9	37.663522091309524	39.0	37.0	39.0	35.0	39.0
10	37.00102883028139	39.0	37.0	39.0	33.0	39.0
11	36.81963790648727	39.0	37.0	39.0	32.0	39.0
12	36.712580805071376	39.0	35.0	39.0	32.0	39.0
13	36.70933040907854	39.0	35.0	39.0	32.0	39.0
14	37.79566302303184	40.0	37.0	41.0	33.0	41.0
15	37.79969722613069	40.0	37.0	41.0	33.0	41.0
16	37.80470951901684	40.0	37.0	41.0	33.0	41.0
17	37.77191213215228	40.0	36.0	41.0	33.0	41.0
18	37.73916682105797	39.0	36.0	41.0	33.0	41.0
19	37.713157644372565	39.0	36.0	41.0	33.0	41.0
20	37.621916555254565	39.0	36.0	41.0	32.0	41.0
21	37.56589604541274	39.0	36.0	41.0	32.0	41.0
22	37.552691499465055	39.0	36.0	41.0	32.0	41.0
23	37.49844256726871	39.0	36.0	41.0	32.0	41.0
24	37.49870327993898	39.0	36.0	41.0	32.0	41.0
25	37.44201012473148	39.0	36.0	41.0	32.0	41.0
26	37.37457901246314	39.0	36.0	41.0	32.0	41.0
27	37.33152002830786	39.0	36.0	41.0	32.0	41.0
28	37.262724931441916	39.0	36.0	41.0	32.0	41.0
29	37.19115713362943	39.0	35.0	41.0	31.0	41.0
30	37.10196369050301	39.0	35.0	41.0	31.0	41.0
31	36.993977570698696	39.0	35.0	41.0	31.0	41.0
32	36.8771909795419	39.0	35.0	41.0	31.0	41.0
33	36.79708041867584	39.0	35.0	41.0	31.0	41.0
34	36.676536026585346	39.0	35.0	41.0	30.0	41.0
35	36.58975976712784	39.0	35.0	40.0	30.0	41.0
36	36.539970657306384	39.0	35.0	40.0	30.0	41.0
37	36.42562361569417	39.0	35.0	40.0	30.0	41.0
38	36.34621140425986	39.0	35.0	40.0	30.0	41.0
39	36.28832684889848	39.0	35.0	40.0	30.0	41.0
40	36.213567741063244	39.0	35.0	40.0	29.0	41.0
41	36.16413782052887	38.0	35.0	40.0	29.0	41.0
42	36.11906223555273	38.0	35.0	40.0	29.0	41.0
43	36.03971011154563	38.0	35.0	40.0	29.0	41.0
44	35.920424217236075	38.0	35.0	40.0	29.0	41.0
45	35.81269580573067	38.0	34.0	40.0	28.0	41.0
46	35.69944268911266	38.0	34.0	40.0	28.0	41.0
47	35.58471442615672	38.0	34.0	40.0	28.0	41.0
48	35.49409724482455	38.0	34.0	40.0	28.0	41.0
49	35.40379185047578	38.0	34.0	40.0	28.0	41.0
50	35.22858625967449	37.0	34.0	40.0	27.0	41.0
51	33.96307861271483	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	37.0
9	88.0
10	174.0
11	131.0
12	78.0
13	42.0
14	57.0
15	91.0
16	175.0
17	400.0
18	779.0
19	1428.0
20	2544.0
21	4031.0
22	6385.0
23	9445.0
24	14176.0
25	20024.0
26	26627.0
27	33074.0
28	40057.0
29	49169.0
30	62400.0
31	78692.0
32	99644.0
33	131962.0
34	197409.0
35	233997.0
36	271753.0
37	381959.0
38	604278.0
39	724406.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.661706115731725	20.91977160101281	26.149247154609956	10.269275128645512
2	32.29599066641964	24.139356096453675	25.50921590914781	18.055437327978876
3	31.97004975572792	24.74470354365602	26.410794372478623	16.87445232813744
4	28.436942417884687	26.814648647114886	26.19811826207131	18.550290672929112
5	25.148791491620308	30.54774697184403	24.78823354647679	19.515227990058868
6	26.245387038140493	35.65885029384421	23.36623119972894	14.729531468286355
7	83.49949176051155	5.650387981179283	6.922639106566721	3.927481151742452
8	84.98865849811476	4.3000565823272865	6.5938607340346875	4.117424185523269
9	78.34782942514693	7.446167507056101	8.91186676614474	5.294136301652237
10	38.63621569383453	31.019666948743758	15.995406650009098	14.348710707412618
11	27.721634978894294	26.375509699946754	26.479627858534165	19.423227462624787
12	25.284789368531214	24.064447103869462	30.447467732217042	20.20329579538228
13	24.257995383282676	24.253755881474213	31.71087265304351	19.7773760821996
14	21.484827090082735	27.290140487743	29.723180561049663	21.501851861124603
15	20.804937851240222	26.633351526471017	31.670981277759143	20.890729344529625
16	23.955588714913535	26.620933458181657	29.205794430896955	20.217683396007857
17	23.9954467082939	25.78712025997827	28.338365655361887	21.879067376365946
18	24.32098703613758	25.383165839629996	29.48703697212778	20.808810152104645
19	23.373408309089726	27.25682534754735	28.117243923241652	21.252522420121274
20	24.671229972943966	27.159884298320726	28.0000066763808	20.16887905235451
21	24.03704056068246	27.88924551889666	28.5428631992883	19.53085072113258
22	23.014953423898437	25.232947271613533	28.022572843487275	23.729526461000756
23	21.88858121900699	27.28596774974254	28.328351084160786	22.497099947089684
24	21.9562129565184	26.394070038572792	29.053572948640273	22.59614405626854
25	21.82719189754426	27.7171951856618	27.094589294089566	23.36102362270437
26	21.69973978805829	27.89909318057774	27.936380767349828	22.46478626401414
27	22.47710418659149	26.829804031532547	28.05184877329848	22.64124300857748
28	20.49331777736607	27.60610020913763	28.472494145648586	23.42808786784772
29	22.990651397783775	26.343362926391233	27.337609555236202	23.32837612058879
30	24.08978396900824	25.993286899104863	26.983127116621354	22.933802015265545
31	24.16916613672894	27.84354569231565	25.49142335431386	22.495864816641546
32	23.67117489280236	27.8859407104003	26.069664695465235	22.373219701332108
33	23.386627543075175	27.537066431658065	25.250339243599434	23.825966781667326
34	22.9826731227269	27.412852366860445	26.7846383154156	22.819836194997052
35	23.018859106666866	26.987266472717803	26.398776887037307	23.59509753357802
36	23.7490882567469	28.889300599038272	25.322577683863358	22.03903346035148
37	22.56216127799281	29.250426036549847	26.356615542280686	21.830797143176657
38	22.677796193461486	29.420339927928467	26.201589980087693	21.700273898522347
39	23.507069452720376	27.87509159159911	26.17067833698031	22.44716061870021
40	23.581410952936523	26.68365805580453	28.190750875857706	21.544180115401243
41	21.142996393085273	27.411116507852256	27.446134125152096	23.999752973910375
42	22.653427403538814	27.200510075493177	27.25402126761104	22.892041253356968
43	23.03805370146897	25.965813592109853	27.855863614893	23.14026909152817
44	23.314422484715262	26.10047619286061	26.209801928472594	24.375299393951533
45	23.722583024967996	25.481709220248796	26.174817693076758	24.62089006170645
46	23.584682379528882	26.64556930333635	26.384856633067784	23.384891684066982
47	21.516272843654182	27.08193755247218	28.500468181203654	22.901321422669984
48	21.413356433610904	26.756163551300478	28.48868436909036	23.34179564599826
49	23.339358767005994	25.48982102292168	28.15756926327807	23.013250946794255
50	21.66231867367019	25.642376324218404	28.20764211928356	24.48766288282785
51	21.626299599250242	24.56851385432471	26.91870004189429	26.886486504530758
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	377.0
1	877.0
2	1377.0
3	5933.0
4	10489.0
5	7637.5
6	4786.0
7	4665.5
8	4545.0
9	4819.0
10	5093.0
11	5281.0
12	5469.0
13	5735.5
14	6002.0
15	6300.5
16	6599.0
17	6970.0
18	7341.0
19	7157.0
20	6973.0
21	8461.5
22	9950.0
23	10382.5
24	10815.0
25	15211.5
26	22265.5
27	24923.0
28	29513.0
29	34103.0
30	43846.5
31	53590.0
32	59957.0
33	66324.0
34	74405.0
35	82486.0
36	87668.0
37	92850.0
38	102612.5
39	112375.0
40	122385.0
41	132395.0
42	143415.0
43	154435.0
44	176353.5
45	198272.0
46	227468.0
47	256664.0
48	287438.0
49	318212.0
50	311510.5
51	304809.0
52	272286.5
53	239764.0
54	213498.0
55	187232.0
56	166076.0
57	144920.0
58	135764.5
59	126609.0
60	115428.0
61	104247.0
62	94092.0
63	83937.0
64	72763.5
65	61590.0
66	51022.0
67	40454.0
68	33492.5
69	26531.0
70	22496.0
71	18461.0
72	15462.5
73	12464.0
74	10294.5
75	6645.5
76	5166.0
77	4044.5
78	2923.0
79	2131.0
80	1339.0
81	981.0
82	623.0
83	412.0
84	201.0
85	151.5
86	102.0
87	71.0
88	40.0
89	31.0
90	22.0
91	20.5
92	19.0
93	10.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2995635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.03538836215441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.59045854967695	17.597438379169393
2	12.992504836646981	6.765298184385474
3	5.304176488853901	4.142888844861596
4	2.82213497031308	2.9390151985007256
5	1.6822084443358802	2.1898475077190125
6	1.086719662722104	1.6975901075855657
7	0.7253851176636805	1.3219978275350697
8	0.583533679200778	1.2154020768311264
9	0.44301976632772844	1.0380772501607975
>10	5.5957954844260405	36.347519036345936
>50	1.0770657448959449	18.121008839190004
>100	0.08850312235901585	3.9117035677739658
>500	0.005920153008903452	1.067039295256387
>1k	0.002187882633725101	1.0355759604080046
>5k	3.860969353632531E-4	0.6095979242769308
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	6744	0.22512756060067396	Illumina PCR Primer Index 9 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT	5894	0.19675294219756412	RNA PCR Primer, Index 17 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5555	0.18543647674032385	No Hit
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	4395	0.1467134680960798	RNA PCR Primer, Index 17 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	3170	0.10582063569159793	Illumina PCR Primer Index 9 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.338190400365866E-5	0.0	0.0	0.2173495769678215	0.0
2	3.338190400365866E-5	0.0	0.0	0.8214285118180286	0.0
3	3.338190400365866E-5	0.0	0.0	1.1760110961448909	0.0
4	3.338190400365866E-5	0.0	0.0	1.5737564823484838	0.0
5	3.338190400365866E-5	0.0	0.0	2.7046018623764243	0.0
6	3.338190400365866E-5	0.0	0.0	3.500226162399625	0.0
7	3.338190400365866E-5	0.0	0.0	4.299589235671235	0.0
8	3.338190400365866E-5	0.0	0.0	5.637335656713852	0.0
9	3.338190400365866E-5	0.0	0.0	6.168208076084036	0.0
10	3.338190400365866E-5	0.0	0.0	7.371659097319934	0.0
11	3.338190400365866E-5	0.0	0.0	9.094766218180787	0.0
12	3.338190400365866E-5	0.0	0.0	10.350493301086415	0.0
13	3.338190400365866E-5	0.0	0.0	10.887174171753234	0.0
14	3.338190400365866E-5	0.0	0.0	11.137004341316615	0.0
15	3.338190400365866E-5	0.0	0.0	11.543762841601197	0.0
16	6.676380800731732E-5	3.338190400365866E-5	0.0	12.378877934060725	0.0
17	6.676380800731732E-5	3.338190400365866E-5	0.0	13.425934735039483	0.0
18	6.676380800731732E-5	3.338190400365866E-5	0.0	14.533179108936837	0.0
19	6.676380800731732E-5	3.338190400365866E-5	0.0	15.241810167126502	0.0
20	6.676380800731732E-5	3.338190400365866E-5	0.0	15.926272726817519	0.0
21	1.669095200182933E-4	3.338190400365866E-5	0.0	16.785088971119645	0.0
22	2.0029142402195195E-4	3.338190400365866E-5	0.0	17.70015372366794	0.0
23	2.0029142402195195E-4	3.338190400365866E-5	0.0	18.613549381016046	0.0
24	3.672009440402452E-4	3.338190400365866E-5	0.0	19.329324166662495	0.0
25	4.673466560512212E-4	3.338190400365866E-5	0.0	19.992455689695174	0.0
26	4.673466560512212E-4	3.338190400365866E-5	0.0	20.613559395587245	0.0
27	4.673466560512212E-4	3.338190400365866E-5	0.0	21.213832793381037	0.0
28	4.673466560512212E-4	3.338190400365866E-5	0.0	21.838808800137535	0.0
29	4.673466560512212E-4	3.338190400365866E-5	0.0	22.48241190932807	0.0
30	4.673466560512212E-4	3.338190400365866E-5	0.0	23.249027334772094	0.0
31	5.007285600548798E-4	3.338190400365866E-5	0.0	23.929417302174663	0.0
32	5.007285600548798E-4	3.338190400365866E-5	0.0	24.57135131616502	0.0
33	8.67929504095125E-4	3.338190400365866E-5	0.0	25.224034303244554	0.0
34	8.67929504095125E-4	3.338190400365866E-5	0.0	25.862797036354564	0.0
35	8.67929504095125E-4	3.338190400365866E-5	0.0	26.546925777005544	0.0
36	9.013114080987837E-4	3.338190400365866E-5	0.0	27.199341708853048	0.0
37	9.013114080987837E-4	3.338190400365866E-5	0.0	27.84514802370783	0.0
38	9.013114080987837E-4	3.338190400365866E-5	0.0	28.503772989700014	0.0
39	9.346933121024424E-4	3.338190400365866E-5	0.0	29.200954054816425	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAA	20	7.0351025E-4	45.000004	25
GCGTAAG	320	0.0	41.48438	1
CGAAAGG	750	0.0	39.0	2
CGTTTTT	4715	0.0	38.319195	1
CGACCAA	525	0.0	37.714283	29
CGAATAT	305	0.0	37.62295	14
ACGATAG	120	0.0	37.500004	1
TCGAAAG	315	0.0	37.142857	1
AACACGT	520	0.0	36.778847	41
TCTAGCG	130	0.0	36.346157	1
GACCGAT	1305	0.0	36.03448	9
ACTATCG	25	0.002107648	36.0	17
CGTAAGG	575	0.0	36.0	2
CGCGTAG	25	0.002107648	36.0	1
ATTAGCG	120	0.0	35.625004	1
ACACGAC	570	0.0	35.526318	26
TTACGAG	95	0.0	35.526314	1
ACGACCA	560	0.0	35.357143	28
TACGGGA	1350	0.0	35.333332	4
TATAGCG	185	0.0	35.27027	1
>>END_MODULE