##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552466_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1273635
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.632562704385478	31.0	31.0	34.0	30.0	34.0
2	31.793366231298606	31.0	31.0	34.0	30.0	34.0
3	31.849174999116702	31.0	31.0	34.0	30.0	34.0
4	35.48424548634421	37.0	35.0	37.0	33.0	37.0
5	35.47515811044766	37.0	35.0	37.0	33.0	37.0
6	35.48724634608816	37.0	35.0	37.0	33.0	37.0
7	35.877103722809125	37.0	35.0	37.0	35.0	37.0
8	35.900233583404976	37.0	35.0	37.0	35.0	37.0
9	37.63381424034358	39.0	37.0	39.0	35.0	39.0
10	37.02309688411515	39.0	37.0	39.0	33.0	39.0
11	36.85186022683108	39.0	37.0	39.0	33.0	39.0
12	36.70663180581564	39.0	35.0	39.0	32.0	39.0
13	36.65121954092028	39.0	35.0	39.0	32.0	39.0
14	37.71695265912133	39.0	36.0	41.0	33.0	41.0
15	37.739300506031945	39.0	36.0	41.0	33.0	41.0
16	37.75765662846891	39.0	36.0	41.0	33.0	41.0
17	37.735293078472246	39.0	36.0	41.0	33.0	41.0
18	37.70801838831376	39.0	36.0	41.0	33.0	41.0
19	37.69266862170088	39.0	36.0	41.0	33.0	41.0
20	37.59377922246169	39.0	36.0	41.0	32.0	41.0
21	37.51685529998783	39.0	36.0	41.0	32.0	41.0
22	37.51310854365654	39.0	36.0	41.0	32.0	41.0
23	37.44642774421243	39.0	36.0	41.0	32.0	41.0
24	37.43852673646688	39.0	36.0	41.0	32.0	41.0
25	37.36997491432004	39.0	36.0	41.0	32.0	41.0
26	37.32781762435863	39.0	36.0	41.0	32.0	41.0
27	37.26964868270737	39.0	35.0	41.0	32.0	41.0
28	37.187510550510936	39.0	35.0	41.0	32.0	41.0
29	37.12218335708425	39.0	35.0	41.0	31.0	41.0
30	37.035179623675546	39.0	35.0	41.0	31.0	41.0
31	36.93960043497548	39.0	35.0	40.0	31.0	41.0
32	36.82856548383171	39.0	35.0	40.0	31.0	41.0
33	36.75260101991544	39.0	35.0	40.0	31.0	41.0
34	36.65211069105356	39.0	35.0	40.0	30.0	41.0
35	36.61565911740805	39.0	35.0	40.0	30.0	41.0
36	36.52170676842267	39.0	35.0	40.0	30.0	41.0
37	36.39508336375806	39.0	35.0	40.0	30.0	41.0
38	36.30985878999871	38.0	35.0	40.0	30.0	41.0
39	36.274230843216465	38.0	35.0	40.0	30.0	41.0
40	36.18897957421082	38.0	35.0	40.0	30.0	41.0
41	36.15174755718868	38.0	35.0	40.0	30.0	41.0
42	36.089621437853076	38.0	35.0	40.0	30.0	41.0
43	35.986374432235294	38.0	35.0	40.0	30.0	41.0
44	35.854488138281376	38.0	35.0	40.0	29.0	41.0
45	35.78543774315247	38.0	35.0	40.0	29.0	41.0
46	35.64757485464831	38.0	34.0	40.0	29.0	41.0
47	35.5333529621909	38.0	34.0	40.0	28.0	41.0
48	35.47178351725573	38.0	34.0	40.0	28.0	41.0
49	35.42724092852348	38.0	34.0	40.0	28.0	41.0
50	35.30786371291618	37.0	34.0	40.0	28.0	41.0
51	34.03866570877842	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	57.0
10	84.0
11	72.0
12	34.0
13	30.0
14	39.0
15	38.0
16	98.0
17	182.0
18	413.0
19	733.0
20	1170.0
21	1894.0
22	2881.0
23	4359.0
24	6172.0
25	8298.0
26	10244.0
27	12637.0
28	15874.0
29	20140.0
30	25769.0
31	33255.0
32	42866.0
33	57479.0
34	90001.0
35	106393.0
36	118610.0
37	164228.0
38	251603.0
39	297928.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.149595449245666	20.88345562111594	25.802918418542202	11.164030511096193
2	29.731202424556486	25.09769282408225	25.679649193057664	19.491455558303596
3	30.657841532307135	24.232609813643627	27.30193501277839	17.80761364127085
4	28.95672622062051	26.751698877621926	25.501026589250454	18.790548312507116
5	25.022003949326145	29.864757171403113	24.25278827921657	20.860450600054175
6	25.018392239534876	34.29169267490293	23.795278867179373	16.894636218382818
7	79.57319012118856	6.604717992203418	8.505890620154126	5.31620126645389
8	79.67761564341433	5.197878513074782	8.466004781589701	6.658501061921195
9	73.8928342892587	7.460928759024367	11.539962391108913	7.106274560608024
10	37.86602912137308	27.392306272990304	18.442567925661592	16.29909667997503
11	28.44409897655137	25.815637918241883	24.63311702332301	21.107146081883744
12	24.620161977332593	22.889053771292403	31.514444876279313	20.97633937509569
13	23.09994621693028	25.02726448315255	31.619812583668004	20.25297671624916
14	19.24876436341652	28.67108708538946	29.280209793229616	22.79993875796441
15	18.54479501584049	26.082433350214153	33.65838721454734	21.714384419398023
16	20.030306956074543	26.848272856823186	29.779881991308343	23.34153819579393
17	19.912926387858374	26.431041860501637	27.46273461392	26.19329713771999
18	21.221857125471583	24.96720017901518	30.71343045692055	23.097512238592692
19	21.45968036368347	26.660699493968053	28.053720257373584	23.825899884974895
20	23.51419362690253	26.98944360040357	28.166311384344812	21.330051388349094
21	22.37155857054808	28.512014823713226	28.23454129322765	20.88188531251104
22	21.02250644807971	25.985623824722154	26.470770668205567	26.52109905899257
23	20.45334809423422	27.478751761689967	27.200336046041446	24.867564098034368
24	21.608702650288347	24.751125714981136	28.389687783391633	25.250483851338885
25	19.895260416053265	27.506624739426915	26.364774837374917	26.233340007144907
26	19.285117007619924	26.94626011376886	29.23490638997829	24.53371648863293
27	22.18155122935535	25.976359003953252	29.357154914869643	22.484934851821752
28	19.510927385004337	25.901769345220572	30.215485598307207	24.371817671467884
29	20.615560973120242	23.820325289427505	30.862923836106894	24.701189901345362
30	21.951422503307462	23.73356573900686	29.22257946742984	25.092432290255847
31	22.69755463692502	25.96835043006827	25.445045087485816	25.88904984552089
32	20.71394080721714	25.078456543672246	28.44621889316798	25.761383755942635
33	20.737181374569637	23.343501081550052	28.030558205451328	27.888759338428986
34	20.45287700165275	22.403435835227516	29.884935636976056	27.258751526143676
35	18.834516953444275	21.548873892441712	31.10726385502911	28.509345299084902
36	21.21447667502856	23.408276311502117	30.753394810915214	24.623852202554104
37	21.610744051474718	22.85623432145002	31.20046167072984	24.33255995634542
38	19.880578030597462	25.09117604337192	28.51845308899331	26.5097928370373
39	21.39639692690606	21.279173389550383	29.549674749830213	27.774754933713346
40	21.246589485998733	21.124340961107382	29.567105175344583	28.061964377549298
41	18.439505823882037	22.331986793704633	28.769152857765373	30.459354524647956
42	21.226096958704808	20.900886046630315	31.935758674973595	25.937258319691274
43	22.685698806958037	20.242377133166094	32.65119127536539	24.420732784510477
44	21.680701299822946	21.011906864996643	29.956620224789678	27.350771610390733
45	21.81771072560035	19.710199546965967	29.023778398049675	29.44831132938401
46	22.105155715727033	20.945168749288452	29.389503272130558	27.560172262853953
47	18.63972017100661	21.402207068744186	32.98307599901071	26.9749967612385
48	19.066530049818038	20.69596077369105	32.02228267910351	28.2152264973874
49	21.495404884444916	19.395980795125762	33.598872518421686	25.50974180200764
50	20.23491816729283	18.799499071555037	32.36500253212263	28.60058022902951
51	19.183518040882987	19.080898373552863	28.37665422197097	33.358929363593184
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	333.0
1	626.5
2	920.0
3	1727.5
4	2535.0
5	2015.5
6	1496.0
7	1558.5
8	1621.0
9	1795.0
10	1969.0
11	2162.0
12	2355.0
13	2470.0
14	2585.0
15	2881.5
16	3178.0
17	3021.5
18	2865.0
19	2955.5
20	3046.0
21	3283.0
22	3520.0
23	4078.5
24	4637.0
25	6213.0
26	8653.0
27	9517.0
28	12078.0
29	14639.0
30	16158.5
31	17678.0
32	19285.5
33	20893.0
34	23626.0
35	26359.0
36	28572.0
37	30785.0
38	33731.0
39	36677.0
40	41075.0
41	45473.0
42	54106.0
43	62739.0
44	73584.5
45	84430.0
46	102429.5
47	120429.0
48	141213.5
49	161998.0
50	159452.5
51	156907.0
52	130424.5
53	103942.0
54	88167.5
55	72393.0
56	63816.0
57	55239.0
58	51081.5
59	46924.0
60	44101.5
61	41279.0
62	38222.0
63	35165.0
64	31685.0
65	28205.0
66	24132.0
67	20059.0
68	17039.5
69	14020.0
70	12295.5
71	10571.0
72	8706.0
73	6841.0
74	5934.0
75	4106.0
76	3185.0
77	2434.0
78	1683.0
79	1225.5
80	768.0
81	604.5
82	441.0
83	341.0
84	241.0
85	204.0
86	167.0
87	116.0
88	65.0
89	48.0
90	31.0
91	18.0
92	5.0
93	4.0
94	3.0
95	5.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1273635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.314263354005796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.4011096848146	16.55580914205616
2	13.16951952702651	6.667533711057418
3	5.922583990644868	4.4977755262620835
4	3.3936397691864	3.4362996338324785
5	2.0466839142153344	2.590514780342719
6	1.3473466082476013	2.046425212218375
7	0.9547895428884401	1.6918855755430147
8	0.682791521026941	1.3827491503326537
9	0.546525302405859	1.2451396891256612
>10	5.348583503930151	33.192267320599754
>50	1.0591887052805653	17.172360826577883
>100	0.11606581937829506	5.437015956356974
>500	0.007448073969608686	1.2798108184913124
>1k	0.002793027738603257	1.1854610061404398
>5k	9.310092462010858E-4	1.6189516510631001
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	7525	0.5908286125930899	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	7519	0.5903575200116202	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT	5564	0.4368598538827843	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	3360	0.26381184562296106	No Hit
ACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	2339	0.18364759134288866	No Hit
GCCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	1619	0.12711648156653987	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1430	0.1122770652502483	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	1403	0.11015714863363522	No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	1360	0.10678098513310329	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4749398375515748	0.0
2	0.0	0.0	0.0	2.245619820435211	0.0
3	0.0	0.0	0.0	3.0329725549313578	0.0
4	0.0	0.0	0.0	3.904729376940803	0.0
5	0.0	0.0	0.0	6.166758922297205	0.0
6	0.0	0.0	0.0	7.382648875070173	0.0
7	0.0	0.0	0.0	8.781322749453336	0.0
8	0.0	0.0	0.0	10.99545788236033	0.0
9	0.0	0.0	0.0	11.759098956922509	0.0
10	0.0	0.0	0.0	13.641584912474924	0.0
11	0.0	0.0	0.0	16.434221735426554	0.0
12	0.0	0.0	0.0	18.49768575769353	0.0
13	0.0	0.0	0.0	19.31440326310128	0.0
14	0.0	0.0	0.0	19.621555626219443	0.0
15	7.851543024492888E-5	0.0	0.0	20.203983087776326	0.0
16	7.851543024492888E-5	0.0	0.0	21.467217844986987	0.0
17	7.851543024492888E-5	0.0	0.0	23.040902613386095	0.0
18	7.851543024492888E-5	0.0	0.0	24.84322431465844	0.0
19	7.851543024492888E-5	0.0	0.0	25.789570795400564	0.0
20	7.851543024492888E-5	0.0	0.0	26.662348318003197	0.0
21	6.28123441959431E-4	0.0	0.0	27.73306324025329	0.0
22	0.001256246883918862	0.0	0.0	28.815476961609882	0.0
23	0.0015703086048985777	0.0	0.0	29.83766934796861	0.0
24	0.002198432046858009	0.0	0.0	30.713587487781037	0.0
25	0.0031406172097971554	0.0	0.0	31.49308867925269	0.0
26	0.003297648070287013	0.0	0.0	32.214566967773344	0.0
27	0.003297648070287013	0.0	0.0	32.88650201980944	0.0
28	0.003297648070287013	0.0	0.0	33.575631951069184	0.0
29	0.003297648070287013	0.0	0.0	34.26805953039921	0.0
30	0.003376163500531942	0.0	0.0	35.05910249011687	0.0
31	0.003376163500531942	0.0	0.0	35.753885532354246	0.0
32	0.003376163500531942	0.0	0.0	36.45675566390685	0.0
33	0.003376163500531942	0.0	0.0	37.11400833048715	0.0
34	0.0035331943610217996	0.0	0.0	37.73922670152752	0.0
35	0.0035331943610217996	0.0	0.0	38.43746442269567	0.0
36	0.0036117097912667286	0.0	0.0	39.08592336108854	0.0
37	0.0037687406517565867	0.0	0.0	39.71404680304797	0.0
38	0.003925771512246444	0.0	0.0	40.310842588339675	0.0
39	0.003925771512246444	0.0	0.0	40.93221370329804	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCC	25	3.891043E-5	45.000004	11
GTTAGTC	20	7.0335733E-4	45.000004	30
GCCGATA	50	2.1827873E-11	45.000004	9
TCGCTAG	40	6.8157533E-9	45.000004	1
AACGCAT	20	7.0335733E-4	45.000004	13
CGAATTA	25	3.891043E-5	45.000004	14
CATCGTA	25	3.891043E-5	45.000004	11
TTAGTCG	20	7.0335733E-4	45.000004	1
GACGAAC	25	3.891043E-5	45.000004	9
TACGTTG	25	3.891043E-5	45.000004	1
ATACACG	20	7.0335733E-4	45.000004	1
TAACTAC	20	7.0335733E-4	45.000004	33
ACGCGTA	40	6.8157533E-9	45.000004	23
TCCCGTA	60	3.6379788E-12	41.250004	25
GCTACGA	175	0.0	41.142857	10
TACGAAT	170	0.0	41.02941	12
TAGTTAG	110	0.0	40.909092	1
ATATGCG	165	0.0	40.90909	1
CATACGC	50	1.0822987E-9	40.500004	20
TGATTCG	45	1.9286745E-8	40.0	15
>>END_MODULE