FastQCFastQC Report
Sat 18 Jun 2016
SRR3552465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634672
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC66920.40937876222263553No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC47130.28831472001722674No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT46800.28629596640794No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC31700.1939226951951217No Hit
ACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC21340.1305460667338769No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA17160.10497518768291131No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC207.034162E-445.032
ACGCTAG351.2123564E-745.01
TAGTCCG502.1827873E-1145.01
GCGTACG700.045.01
AACGCGA302.1660671E-644.99999634
AATACGG1100.042.9545442
TACGAAT2650.041.60377512
TCTACGG850.039.7058832
GAGTACG1250.039.61
CGTAAGG2050.039.5121962
TAGATCG403.4594086E-739.3751
GCTACGA2800.039.37510
CCGCATT403.4594086E-739.37513
AACACGT1950.039.2307741
TTTGACG1550.039.1935461
ATCGTTG1800.038.7523
CGAAAGG3150.038.571432
AATCGTT1650.038.1818222
TCATGCG659.094947E-1238.0769271
CGAATAT2900.038.0172414
Produced by FastQC (version 0.11.5)