##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552465_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1634672
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63686598901798	31.0	31.0	34.0	30.0	34.0
2	31.802595872444137	31.0	31.0	34.0	30.0	34.0
3	31.86089319447571	33.0	31.0	34.0	30.0	34.0
4	35.48188627443304	37.0	35.0	37.0	33.0	37.0
5	35.46019017882487	37.0	35.0	37.0	33.0	37.0
6	35.46697257920855	37.0	35.0	37.0	33.0	37.0
7	35.874913132420446	37.0	35.0	37.0	35.0	37.0
8	35.881201855785136	37.0	35.0	37.0	35.0	37.0
9	37.586690785674435	39.0	37.0	39.0	35.0	39.0
10	36.97086449146985	39.0	37.0	39.0	33.0	39.0
11	36.8103038407705	39.0	37.0	39.0	32.0	39.0
12	36.591603697867214	39.0	35.0	39.0	32.0	39.0
13	36.54844580441826	39.0	35.0	39.0	32.0	39.0
14	37.60525536621414	39.0	36.0	41.0	33.0	41.0
15	37.61069743654996	39.0	36.0	41.0	33.0	41.0
16	37.63593980933178	39.0	36.0	41.0	33.0	41.0
17	37.60779226658315	39.0	36.0	41.0	33.0	41.0
18	37.57813799954976	39.0	36.0	41.0	32.0	41.0
19	37.57157766206309	39.0	36.0	41.0	32.0	41.0
20	37.475403016629635	39.0	36.0	41.0	32.0	41.0
21	37.43236624839723	39.0	36.0	41.0	32.0	41.0
22	37.400639394324976	39.0	36.0	41.0	32.0	41.0
23	37.35321214286414	39.0	35.0	41.0	32.0	41.0
24	37.33873033856333	39.0	35.0	41.0	32.0	41.0
25	37.26527768261767	39.0	35.0	41.0	32.0	41.0
26	37.200420634842956	39.0	35.0	41.0	32.0	41.0
27	37.127505701449586	39.0	35.0	41.0	31.0	41.0
28	37.053482900545184	39.0	35.0	41.0	31.0	41.0
29	37.02366040404436	39.0	35.0	41.0	31.0	41.0
30	36.94346082883906	39.0	35.0	41.0	31.0	41.0
31	36.844367555081384	39.0	35.0	41.0	31.0	41.0
32	36.75615108107314	39.0	35.0	40.0	31.0	41.0
33	36.69092637544413	39.0	35.0	40.0	30.0	41.0
34	36.566259775661415	39.0	35.0	40.0	30.0	41.0
35	36.51727318997328	39.0	35.0	40.0	30.0	41.0
36	36.46599623655388	39.0	35.0	40.0	30.0	41.0
37	36.3756025673652	39.0	35.0	40.0	30.0	41.0
38	36.30785870192919	38.0	35.0	40.0	30.0	41.0
39	36.29145908169957	38.0	35.0	40.0	30.0	41.0
40	36.17943477345914	38.0	35.0	40.0	30.0	41.0
41	36.084230353245175	38.0	35.0	40.0	30.0	41.0
42	36.030527225033524	38.0	35.0	40.0	29.0	41.0
43	35.96792567560954	38.0	35.0	40.0	29.0	41.0
44	35.875554851370794	38.0	35.0	40.0	29.0	41.0
45	35.795102014349055	38.0	34.0	40.0	29.0	41.0
46	35.65185676392573	38.0	34.0	40.0	28.0	41.0
47	35.54123824228959	38.0	34.0	40.0	28.0	41.0
48	35.480382608865874	38.0	34.0	40.0	28.0	41.0
49	35.42776349016806	38.0	34.0	40.0	28.0	41.0
50	35.311355427877885	37.0	34.0	40.0	28.0	41.0
51	34.0578146563959	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	23.0
9	87.0
10	106.0
11	108.0
12	57.0
13	58.0
14	46.0
15	63.0
16	125.0
17	249.0
18	538.0
19	937.0
20	1574.0
21	2565.0
22	3742.0
23	5484.0
24	7922.0
25	10536.0
26	13637.0
27	17465.0
28	21928.0
29	27646.0
30	35179.0
31	44408.0
32	57052.0
33	75701.0
34	118035.0
35	136894.0
36	147022.0
37	203739.0
38	313930.0
39	387764.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00575283604295	20.703480575919816	25.9359064081357	10.354860179901534
2	30.70842346354498	24.547615668464378	25.75171043487623	18.99225043311441
3	32.24035158123464	24.119150508481212	26.104625270390635	17.53587263989351
4	29.968764375972672	25.737579159611222	25.06380484892382	19.229851615492283
5	25.717819843980934	29.62374103184003	24.061830140847828	20.596608983331212
6	25.817166991298564	35.01014270752787	23.658935859915626	15.513754441257941
7	81.75780829426331	5.881179833018489	7.692980610177455	4.668031262540742
8	81.71988019614945	5.277511329489952	7.4876794855481705	5.514928988812434
9	75.45054910098172	7.640248318928812	10.226761087239518	6.682441492849941
10	39.61161627531395	26.47038671978232	17.211709749723493	16.706287255180243
11	30.87848816153944	25.76498526921609	22.748233284720115	20.608293284524358
12	27.517324576428788	23.14727358148913	29.23681325672673	20.09858858535535
13	25.31853484980473	24.097372439241635	30.22997885814402	20.354113852809615
14	21.267569273835974	27.221485411140584	28.628006107647288	22.882939207376157
15	20.699932463515616	25.846041285346537	32.27436452083354	21.179661730304307
16	22.7627927804477	25.265680209852498	30.14513003220218	21.826396977497627
17	23.036180958626563	24.562297512895555	27.82796793485176	24.573553593626123
18	23.569988352403417	25.616331594350427	28.63100365088532	22.18267640236084
19	24.076083764816428	26.9894510947762	26.587352080417354	22.34711305999002
20	25.491964137148003	26.50366556715965	27.38910313506318	20.615267160629166
21	24.426612800610766	27.060474517212015	27.42917233548993	21.083740346687286
22	22.72621051807335	25.843655485626478	26.339412432585863	25.090721563714315
23	22.872539567570744	26.476137108851194	26.87786907709926	23.773454246478803
24	23.323272191607856	24.808952499339316	27.64780946881087	24.219965840241954
25	22.136245069347247	26.44328648193644	26.312801589554358	25.10766685916196
26	22.387426957823955	26.463351669325714	27.436757955112707	23.712463417737624
27	22.68375551792653	26.05697045034111	26.865756555443536	24.39351747628882
28	19.530340031517028	27.176216390811124	28.42258263431441	24.870860943357446
29	23.414605498840135	24.888846202785636	26.381378037918314	25.315170260455922
30	23.520253604392806	24.60316197989566	26.324975285561873	25.551609130149654
31	23.016238119940883	26.470019673671537	25.707359029823717	24.806383176563862
32	23.85463261131285	26.82935781612458	24.750163947262816	24.565845625299755
33	22.041914218876936	25.259379251617446	26.073365176622588	26.625341352883027
34	21.794402791508023	24.29777961572719	29.02857576321121	24.879241829553575
35	21.952905537012928	24.916313486742293	26.107989859739444	27.022791116505328
36	21.764488533479497	25.987354037996614	27.549257588066595	24.69889984045729
37	21.590508676970057	26.09599968678732	28.933877866630127	23.379613769612497
38	20.54669071226521	27.54136609668484	26.295305724940537	25.61663746610941
39	22.09764405336361	23.19401078626171	28.590322706940597	26.11802245343408
40	22.60159836346374	23.383161882016697	29.71085330879834	24.30438644572122
41	19.817492438850117	25.765168792271474	28.497521215265202	25.9198175536132
42	21.70013311538951	24.462583319467146	28.984285532510494	24.852998032632847
43	22.09226071040551	22.916156880401694	28.856186439848486	26.13539596934431
44	21.4008070120489	23.317460628187185	27.61642702633923	27.66530533342469
45	22.124683116857693	21.83379907406501	27.201114351992327	28.840403457084967
46	21.99499349104897	24.053204557244513	27.726907905683834	26.22489404602269
47	19.599711746454336	24.131446553192323	31.368127673319172	24.90071402703417
48	19.586253389059088	23.180307731459276	30.33140593342273	26.902032946058902
49	21.180334648174068	22.130617028981963	31.645125138254038	25.043923184589932
50	19.739801012068476	22.095441776698934	30.21156537825325	27.95319183297934
51	19.65134289937064	21.85429248191686	28.194341127761408	30.30002349095109
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	441.0
1	856.0
2	1271.0
3	2017.0
4	2763.0
5	2155.5
6	1548.0
7	1643.0
8	1738.0
9	1804.5
10	1871.0
11	2134.5
12	2398.0
13	2604.5
14	2811.0
15	3040.5
16	3270.0
17	3097.0
18	2924.0
19	3247.0
20	3570.0
21	3908.5
22	4247.0
23	5172.5
24	6098.0
25	7356.5
26	10849.0
27	13083.0
28	15873.0
29	18663.0
30	20890.0
31	23117.0
32	25502.5
33	27888.0
34	32740.0
35	37592.0
36	40484.5
37	43377.0
38	46411.0
39	49445.0
40	56334.5
41	63224.0
42	70276.0
43	77328.0
44	89127.0
45	100926.0
46	116248.5
47	131571.0
48	155025.0
49	178479.0
50	176151.0
51	173823.0
52	152825.5
53	131828.0
54	118925.0
55	106022.0
56	95991.5
57	85961.0
58	80691.0
59	75421.0
60	71865.5
61	68310.0
62	62355.5
63	56401.0
64	49634.5
65	42868.0
66	35299.5
67	27731.0
68	23788.5
69	19846.0
70	16735.5
71	13625.0
72	11419.5
73	9214.0
74	7837.5
75	5297.5
76	4134.0
77	3249.0
78	2364.0
79	1861.0
80	1358.0
81	969.5
82	581.0
83	407.5
84	234.0
85	177.0
86	120.0
87	94.5
88	69.0
89	44.0
90	19.0
91	14.5
92	10.0
93	10.5
94	11.0
95	7.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1634672.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.06926171593264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.99403383306836	15.884276720182356
2	13.195847683550749	6.352286229179328
3	5.765010630347614	4.162786489709116
4	3.172279643382001	3.054177158907474
5	1.8453450842279098	2.2208046894245657
6	1.2359220052168578	1.7848638122426888
7	0.836591867353327	1.4095304023323623
8	0.6151873424173201	1.1845684119177236
9	0.475865377646187	1.0308355484505478
>10	5.3091939259377945	32.985149477328534
>50	1.432361329398447	22.450358207689707
>100	0.11318418209116445	4.633757043848367
>500	0.005863144259231798	0.8915200759080543
>1k	0.0030590317874252862	1.544483368944084
>5k	2.5491931561877384E-4	0.41060236393507915
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	6692	0.40937876222263553	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	4713	0.28831472001722674	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT	4680	0.28629596640794	No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	3170	0.1939226951951217	No Hit
ACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	2134	0.1305460667338769	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1716	0.10497518768291131	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.117435179656837E-5	0.0	0.0	0.31627139878825844	0.0
2	6.117435179656837E-5	0.0	0.0	1.441084205271761	0.0
3	6.117435179656837E-5	0.0	0.0	1.9755033425665822	0.0
4	6.117435179656837E-5	0.0	0.0	2.607923791439506	0.0
5	6.117435179656837E-5	0.0	0.0	4.224088991553045	0.0
6	6.117435179656837E-5	0.0	0.0	5.094416494562823	0.0
7	6.117435179656837E-5	0.0	0.0	6.055282038231523	0.0
8	6.117435179656837E-5	0.0	0.0	7.617063239597913	0.0
9	6.117435179656837E-5	0.0	0.0	8.16530778039876	0.0
10	6.117435179656837E-5	0.0	0.0	9.546624643965272	0.0
11	6.117435179656837E-5	0.0	0.0	11.554795090391222	0.0
12	6.117435179656837E-5	0.0	0.0	13.08133986512279	0.0
13	6.117435179656837E-5	0.0	0.0	13.6872718196678	0.0
14	6.117435179656837E-5	0.0	0.0	13.935700862313663	0.0
15	6.117435179656837E-5	0.0	0.0	14.39193917801247	0.0
16	6.117435179656837E-5	0.0	0.0	15.398501962473206	0.0
17	6.117435179656837E-5	0.0	0.0	16.589016022786222	0.0
18	6.117435179656837E-5	0.0	0.0	17.911483159924437	0.0
19	6.117435179656837E-5	0.0	0.0	18.66784284553721	0.0
20	6.117435179656837E-5	0.0	0.0	19.422489649299678	0.0
21	1.8352305538970508E-4	0.0	0.0	20.33906496226766	0.0
22	1.8352305538970508E-4	0.0	0.0	21.340794972936468	0.0
23	2.4469740718627346E-4	0.0	0.0	22.2565138449793	0.0
24	4.2822046257597854E-4	0.0	0.0	22.98956610255758	0.0
25	0.0011011383323382305	0.0	0.0	23.657161803713528	0.0
26	0.0011011383323382305	0.0	0.0	24.263093758258538	0.0
27	0.001162312684134799	0.0	0.0	24.844494797733123	0.0
28	0.001162312684134799	0.0	0.0	25.442290563489188	0.0
29	0.001162312684134799	0.0	0.0	26.064678418667476	0.0
30	0.0012234870359313674	0.0	0.0	26.7559485939687	0.0
31	0.0012234870359313674	0.0	0.0	27.40464142041951	0.0
32	0.0012846613877279356	0.0	0.0	28.077681638885355	0.0
33	0.001345835739524504	0.0	0.0	28.70863390331516	0.0
34	0.0014070100913210724	0.0	0.0	29.32257969794552	0.0
35	0.0014681844431176407	0.0	0.0	29.954938972466646	0.0
36	0.0014681844431176407	0.0	0.0	30.59249806689048	0.0
37	0.0014681844431176407	0.0	0.0	31.205036851429522	0.0
38	0.0014681844431176407	0.0	0.0	31.84632758131295	0.0
39	0.0014681844431176407	0.0	0.0	32.47079536445232	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	20	7.034162E-4	45.0	32
ACGCTAG	35	1.2123564E-7	45.0	1
TAGTCCG	50	2.1827873E-11	45.0	1
GCGTACG	70	0.0	45.0	1
AACGCGA	30	2.1660671E-6	44.999996	34
AATACGG	110	0.0	42.954544	2
TACGAAT	265	0.0	41.603775	12
TCTACGG	85	0.0	39.705883	2
GAGTACG	125	0.0	39.6	1
CGTAAGG	205	0.0	39.512196	2
TAGATCG	40	3.4594086E-7	39.375	1
GCTACGA	280	0.0	39.375	10
CCGCATT	40	3.4594086E-7	39.375	13
AACACGT	195	0.0	39.23077	41
TTTGACG	155	0.0	39.193546	1
ATCGTTG	180	0.0	38.75	23
CGAAAGG	315	0.0	38.57143	2
AATCGTT	165	0.0	38.18182	22
TCATGCG	65	9.094947E-12	38.076927	1
CGAATAT	290	0.0	38.01724	14
>>END_MODULE