FastQCFastQC Report
Sat 18 Jun 2016
SRR3552464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657342
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC55790.3366233402641096No Hit
CTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGCT32920.19863130241072752No Hit
CCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC28620.17268614444091807No Hit
TCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC22590.13630258570651077No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAA207.0341915E-445.011
ATTCACG253.8915554E-545.01
TTGTTCG207.0341915E-445.015
GTATACG603.6379788E-1241.2500041
TCGAACG603.6379788E-1241.2500041
ACGGGTA2100.039.6428575
TATTACG403.4594632E-739.3751
TATAGCG800.039.3751
TCGACGT403.4594632E-739.37526
ATTACGG1800.038.752
TTAACGG1000.038.252
TTGGACG950.037.8947371
AGTACGG950.037.8947372
CGTACTG301.1401393E-437.50000417
TATCGGC301.1401393E-437.50000428
CGAATAT1500.037.514
AACACGT5000.036.89999841
AGGGATC7450.036.5436256
CGTTAGG2550.036.176472
CTCCGAT250.00210737736.019