##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552464_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1657342
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71641459638385	31.0	31.0	34.0	30.0	34.0
2	31.87006725226296	33.0	31.0	34.0	30.0	34.0
3	31.942559833757908	33.0	31.0	34.0	30.0	34.0
4	35.54923003218406	37.0	35.0	37.0	33.0	37.0
5	35.52190676396302	37.0	35.0	37.0	33.0	37.0
6	35.53039445087375	37.0	35.0	37.0	33.0	37.0
7	35.89808621274305	37.0	35.0	37.0	35.0	37.0
8	35.90003873672423	37.0	35.0	37.0	35.0	37.0
9	37.6125687999218	39.0	37.0	39.0	35.0	39.0
10	37.01503250385255	39.0	37.0	39.0	33.0	39.0
11	36.83353164283533	39.0	37.0	39.0	33.0	39.0
12	36.64182830097831	39.0	35.0	39.0	32.0	39.0
13	36.58112930222006	39.0	35.0	39.0	32.0	39.0
14	37.678266163531724	40.0	36.0	41.0	33.0	41.0
15	37.69517395926731	40.0	36.0	41.0	33.0	41.0
16	37.7099880410923	40.0	36.0	41.0	33.0	41.0
17	37.66631570309568	39.0	36.0	41.0	33.0	41.0
18	37.64193690861633	39.0	36.0	41.0	33.0	41.0
19	37.64689183041279	39.0	36.0	41.0	33.0	41.0
20	37.54001226059558	39.0	36.0	41.0	32.0	41.0
21	37.478238046220994	39.0	36.0	41.0	32.0	41.0
22	37.452864285102294	39.0	36.0	41.0	32.0	41.0
23	37.402684539461376	39.0	36.0	41.0	32.0	41.0
24	37.38366070491184	39.0	35.0	41.0	32.0	41.0
25	37.32509886311938	39.0	35.0	41.0	32.0	41.0
26	37.257424237121846	39.0	35.0	41.0	32.0	41.0
27	37.18067363284102	39.0	35.0	41.0	32.0	41.0
28	37.10005961352575	39.0	35.0	41.0	31.0	41.0
29	37.0517093032096	39.0	35.0	41.0	31.0	41.0
30	36.9614774741725	39.0	35.0	41.0	31.0	41.0
31	36.85217655740336	39.0	35.0	41.0	31.0	41.0
32	36.74580261647868	39.0	35.0	40.0	31.0	41.0
33	36.663196853757405	39.0	35.0	40.0	30.0	41.0
34	36.558432116002614	39.0	35.0	40.0	30.0	41.0
35	36.48784439180326	39.0	35.0	40.0	30.0	41.0
36	36.40248783896142	39.0	35.0	40.0	30.0	41.0
37	36.321938984228964	38.0	35.0	40.0	30.0	41.0
38	36.24345488137029	38.0	35.0	40.0	30.0	41.0
39	36.214446384632744	38.0	35.0	40.0	30.0	41.0
40	36.0800727912525	38.0	35.0	40.0	30.0	41.0
41	35.97791222330696	38.0	35.0	40.0	29.0	41.0
42	35.92280651790638	38.0	35.0	40.0	29.0	41.0
43	35.86335167997915	38.0	35.0	40.0	29.0	41.0
44	35.77463432411657	38.0	34.0	40.0	29.0	41.0
45	35.682475312880506	38.0	34.0	40.0	28.0	41.0
46	35.52837857243707	38.0	34.0	40.0	28.0	41.0
47	35.41012898967141	38.0	34.0	40.0	28.0	41.0
48	35.32173081958944	37.0	34.0	40.0	28.0	41.0
49	35.259908938529286	37.0	34.0	40.0	27.0	41.0
50	35.16053536325031	37.0	34.0	40.0	27.0	41.0
51	33.96165486664792	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	27.0
9	64.0
10	114.0
11	85.0
12	63.0
13	34.0
14	43.0
15	64.0
16	161.0
17	273.0
18	550.0
19	957.0
20	1594.0
21	2582.0
22	3881.0
23	5679.0
24	8131.0
25	10814.0
26	14032.0
27	17748.0
28	22236.0
29	28027.0
30	35463.0
31	44719.0
32	57001.0
33	76236.0
34	119896.0
35	139631.0
36	149867.0
37	207747.0
38	319983.0
39	389572.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.73596879823235	20.436699244935568	24.983437335202993	9.84389462162909
2	30.79479069498027	24.139314637534078	25.835524592992876	19.23037007449277
3	32.886935828573705	24.66847518496484	25.573659510227824	16.87092947623363
4	29.198560104070253	26.455372518164626	24.958698928766665	19.38736844899846
5	25.786289130426916	30.404828936936372	23.2955539653252	20.513327967311515
6	26.495798694536195	35.73619687427218	22.38324980601469	15.38475462517694
7	83.19966548847492	5.94928506005399	6.469213958253637	4.381835493217453
8	83.8365889478454	5.000416329279051	6.354270874689714	4.80872384818583
9	77.72004812525115	7.737087456903886	8.562686518533894	5.980177899311066
10	40.70360854911056	27.53221724906507	15.838372526611888	15.925801675212478
11	31.651704958904077	26.514201655421754	21.811309916722077	20.022783468952092
12	28.31111502634942	23.57021061434514	27.75643168398556	20.36224267531988
13	25.078650031194528	24.749870575898033	28.989430063318256	21.182049329589187
14	21.23587044798237	27.173329343008263	28.408318862371196	23.18248134663817
15	20.678652927398208	26.034457583286972	31.729842120696876	21.557047368617944
16	23.20625435184772	26.10330275827198	29.11233770700314	21.57810518287716
17	23.079666115985717	25.031707396542174	27.354704098490235	24.53392238898188
18	23.882216223326267	25.113102787475366	28.605441725365072	22.399239263833294
19	25.152442887466798	26.604104644665977	26.08224494401276	22.16120752385446
20	26.22717580318365	26.499660299443324	26.114404872379993	21.15875902499303
21	25.946907759533033	27.118301473081598	26.23489901299792	20.699891754387448
22	23.779340655097137	25.95994067609461	25.32500835675437	24.935710312053878
23	23.43439072925202	26.649840527784853	26.728701740497733	23.187067002465394
24	23.861279084220396	25.43138350443059	26.972043187223882	23.735294224125138
25	23.284451851217188	26.771782770242957	25.261050525479956	24.6827148530599
26	21.975005762238574	27.843921170162826	25.89387102963661	24.28720203796199
27	23.181274595104693	27.352954308766687	26.342722262514318	23.123048833614305
28	19.822885077431213	28.69733585463954	26.754888248774243	24.724890819155007
29	21.988581716990215	27.244588021060228	25.35982313849525	25.4070071234543
30	23.35124554859528	26.547930360782505	25.440192790624987	24.660631299997224
31	23.904963489732356	28.79695319372827	22.903118366637663	24.39496494990171
32	23.238836643251666	28.350817151800893	23.539016087204693	24.871330117742747
33	22.69211786100877	28.11435418881559	23.809026742820734	25.38450120735491
34	22.421081466589275	26.916834304567196	25.541197893977223	25.12088633486631
35	21.560365935335014	26.959734321582392	24.873562608079684	26.606337135002917
36	21.445362514194414	30.685277993317012	24.260834516955462	23.60852497553311
37	22.555392912265543	29.70328393294806	24.814612795669213	22.926710359117187
38	21.25590252343813	29.447392270273724	24.181128578169144	25.115576628119
39	23.317034142621136	25.200471598499284	25.50409028432273	25.97840397455685
40	24.46018987028628	24.77485033264106	26.789944380821822	23.975015416250837
41	20.552245704266227	27.465785577147024	26.025949984975945	25.956018733610808
42	21.987133615150043	27.150401063872152	26.378381770328634	24.48408355064917
43	22.31235315342277	25.42148814185606	27.109130161427153	25.157028543294018
44	21.9276407645495	25.75364650144629	25.239932373644063	27.078780360360145
45	23.012269042840884	24.069805749205656	24.97203353321161	27.945891674741848
46	22.49487432286155	26.613698319357137	24.852323781090444	26.03910357669087
47	21.121832428068558	26.358168682142853	27.88404565865102	24.63595323113757
48	20.997174994660124	25.70827264378746	27.636721931864393	25.657830429688016
49	22.16911174639875	24.875553748109922	28.010754569666368	24.944579935824954
50	20.995063179476535	24.31712947599228	27.622964964382728	27.064842380148455
51	20.797155927985894	23.572865467718792	25.937495097571894	29.692483506723416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	288.0
1	675.0
2	1062.0
3	1904.5
4	2747.0
5	2151.0
6	1555.0
7	1603.0
8	1651.0
9	1775.0
10	1899.0
11	1982.0
12	2065.0
13	2390.5
14	2716.0
15	2866.0
16	3016.0
17	3285.5
18	3555.0
19	3518.5
20	3482.0
21	3949.0
22	4416.0
23	5281.0
24	6146.0
25	7575.0
26	11392.0
27	13780.0
28	16696.5
29	19613.0
30	22071.5
31	24530.0
32	27564.5
33	30599.0
34	35039.0
35	39479.0
36	42806.5
37	46134.0
38	49254.5
39	52375.0
40	59109.5
41	65844.0
42	72352.5
43	78861.0
44	89269.5
45	99678.0
46	112816.5
47	125955.0
48	144537.0
49	163119.0
50	160946.0
51	158773.0
52	144385.5
53	129998.0
54	117497.5
55	104997.0
56	96166.5
57	87336.0
58	82928.5
59	78521.0
60	77762.5
61	77004.0
62	70799.0
63	64594.0
64	55565.5
65	46537.0
66	39458.5
67	32380.0
68	27876.0
69	23372.0
70	19793.0
71	16214.0
72	14228.0
73	12242.0
74	10812.0
75	7578.5
76	5775.0
77	4488.0
78	3201.0
79	2610.5
80	2020.0
81	1432.5
82	845.0
83	572.5
84	300.0
85	218.5
86	137.0
87	106.0
88	75.0
89	51.5
90	28.0
91	26.0
92	24.0
93	16.5
94	9.0
95	8.0
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1657342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.630787118457064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.72388770026541	16.239995601977213
2	12.260269763029884	5.794396495700304
3	4.977162562748698	3.528428069228061
4	2.5919904097566415	2.45003094344166
5	1.6154774922862813	1.908750235743799
6	1.002127093666406	1.4208631209641351
7	0.7081067005237766	1.1713183088061276
8	0.5222240294868484	0.9872451895157246
9	0.39458673891481566	0.8391955704355987
>10	5.4921589493926275	34.409395823532805
>50	1.5929719465751417	24.33638306146282
>100	0.11033286120504618	4.52573029134676
>500	0.005887832335686175	0.9947484113810425
>1k	0.00255992710247225	1.0560279139794977
>5k	2.55992710247225E-4	0.3374909624844829
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	5579	0.3366233402641096	No Hit
CTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGCT	3292	0.19863130241072752	No Hit
CCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	2862	0.17268614444091807	No Hit
TCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	2259	0.13630258570651077	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2067515334795111E-4	0.0	0.0	0.2236713967304274	0.0
2	1.2067515334795111E-4	0.0	0.0	1.0431160255396894	0.0
3	1.2067515334795111E-4	0.0	0.0	1.44653306318189	0.0
4	1.2067515334795111E-4	0.0	0.0	1.9066674228976277	0.0
5	1.2067515334795111E-4	0.0	0.0	3.132606305759463	0.0
6	1.2067515334795111E-4	0.0	0.0	3.840366080145196	0.0
7	1.2067515334795111E-4	0.0	0.0	4.58107017139492	0.0
8	1.2067515334795111E-4	0.0	0.0	5.8322301613064775	0.0
9	1.2067515334795111E-4	0.0	0.0	6.287597852464971	0.0
10	1.2067515334795111E-4	0.0	0.0	7.4859624627868	0.0
11	1.2067515334795111E-4	0.0	0.0	9.187120099532866	0.0
12	1.2067515334795111E-4	0.0	0.0	10.412696956934658	0.0
13	1.8101273002192668E-4	0.0	0.0	10.90873217477141	0.0
14	1.8101273002192668E-4	0.0	0.0	11.106398075955354	0.0
15	1.8101273002192668E-4	0.0	0.0	11.492317216362103	0.0
16	1.8101273002192668E-4	0.0	0.0	12.331914595780473	0.0
17	1.8101273002192668E-4	0.0	0.0	13.289532275173139	0.0
18	2.4135030669590222E-4	0.0	0.0	14.397511195637351	0.0
19	2.4135030669590222E-4	0.0	0.0	15.009515235841485	0.0
20	2.4135030669590222E-4	0.0	0.0	15.656213382633156	0.0
21	3.6202546004385336E-4	0.0	0.0	16.410493428634524	0.0
22	4.223630367178289E-4	0.0	0.0	17.201096695793627	0.0
23	9.654012267836089E-4	0.0	0.0	17.99707000727671	0.0
24	0.0016894521468713156	0.0	0.0	18.62844240959319	0.0
25	0.0019911400302411934	0.0	0.0	19.18252237619031	0.0
26	0.0019911400302411934	0.0	0.0	19.684832702001156	0.0
27	0.0019911400302411934	0.0	0.0	20.190280581799048	0.0
28	0.0019911400302411934	0.0	0.0	20.732172357908023	0.0
29	0.0019911400302411934	0.0	0.0	21.277382700734066	0.0
30	0.0019911400302411934	0.0	0.0	21.947431489698566	0.0
31	0.0019911400302411934	0.0	0.0	22.49734816350518	0.0
32	0.0019911400302411934	0.0	0.0	23.0555914228928	0.0
33	0.0021118151835891447	0.0	0.0	23.61624818534738	0.0
34	0.0021118151835891447	0.0	0.0	24.135211682320246	0.0
35	0.0021118151835891447	0.0	0.0	24.721089551824548	0.0
36	0.0021118151835891447	0.0	0.0	25.268532384987527	0.0
37	0.0021118151835891447	0.0	0.0	25.78779756984376	0.0
38	0.0021118151835891447	0.0	0.0	26.344110026777816	0.0
39	0.0022324903369370956	0.0	0.0	26.893061299357647	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAA	20	7.0341915E-4	45.0	11
ATTCACG	25	3.8915554E-5	45.0	1
TTGTTCG	20	7.0341915E-4	45.0	15
GTATACG	60	3.6379788E-12	41.250004	1
TCGAACG	60	3.6379788E-12	41.250004	1
ACGGGTA	210	0.0	39.642857	5
TATTACG	40	3.4594632E-7	39.375	1
TATAGCG	80	0.0	39.375	1
TCGACGT	40	3.4594632E-7	39.375	26
ATTACGG	180	0.0	38.75	2
TTAACGG	100	0.0	38.25	2
TTGGACG	95	0.0	37.894737	1
AGTACGG	95	0.0	37.894737	2
CGTACTG	30	1.1401393E-4	37.500004	17
TATCGGC	30	1.1401393E-4	37.500004	28
CGAATAT	150	0.0	37.5	14
AACACGT	500	0.0	36.899998	41
AGGGATC	745	0.0	36.543625	6
CGTTAGG	255	0.0	36.17647	2
CTCCGAT	25	0.002107377	36.0	19
>>END_MODULE