FastQCFastQC Report
Sat 18 Jun 2016
SRR3552463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697689
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC51000.3004083786841995TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT36610.21564609301232437TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC33140.19520654254106612TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC22040.12982354247450506TruSeq Adapter, Index 15 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC20160.11874966498575416No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAC207.0342375E-445.00000410
GCTCGAT207.0342375E-445.0000048
CGCTCGA253.8915954E-545.024
ACGAGTA253.8915954E-545.023
TAAGACG800.042.1875041
CGATAAC800.042.18750410
CTTACCG603.6379788E-1241.2499961
CGTCAAT501.0822987E-940.511
CGTAAGG3450.040.434782
TTAGCCG451.9292202E-840.01
ATCGTCC356.250053E-638.57142619
TTACCGG1300.038.076922
TAGCGCG1850.037.70271
CGCATAA301.14015136E-437.49999634
CCCTCGT3850.037.402614
CGTGTAC3550.037.39436318
GCGTAAG2300.037.1739161
CACAACG3150.037.14285712
TACGGGT1700.037.0588234
ACGGGAT3650.036.98635
Produced by FastQC (version 0.11.5)